Koi Magur breeding technique

1. WELCOME TO OUR PRESENTATION
Topic:
BREEDING TECHNIQUE FOR
KOI(Anabas testudineus) &
MAGUR(Clarias batrachus) OF
BANGLADESH.

2. Content
 Introduction
 BREEDING TECHNIQUE OF KOI (Anabas testudineus)
 MATERIAL AND METHOD
 RESULT AND DISCUSSION
 BREEDING TECHNIQUE OF MAGUR (Clarias batrachus)
 MATERIALS AND METHODS
 Mature brood fish selection
 Hormone extract preparation
 Hormone injection
 Egg collection
 Precautions of during seed production

3. Introduction
 Breeding : Breeding is sexual reproduction that produces
offspring, usually animals or plants. Breeding may refer
to: Breeding in the wild, the natural process of
reproduction in the animal kingdom.
 Fish breeding is the act of producing young ones from
parent brood fish. This ensures species continuity and
survival. In aquaculture, fish breeding is a very important
activity ensuring availability of fingerlings for the stocking
of ponds, pens, tanks and cages.

4. BREEDING TECHNIQUE OF KOI
(Anabas testudineus)
Introduction
 The climbing perch (Anabas testudineus), locally known as koi, is a preferable
small indigenous fish of Bangladesh.
 It can withstand wide range of environmental fluctuations such as low oxygen,
wide range of temperatures and other poor water conditions.
 The species is considered as a valuable item of diet for sick and
convalescents.
 Koi has a great demand in international and domestic markets but the growth
rate of our native koi is poor taking more time to attain marketable size.
 This fish attains 80-100 g in size within 3-4 months.

5. MATERIAL AND METHOD
Selection of broods :
 The female of A. testudineus is larger in size than the male (particularly when
in spawning condition) .
 The females are identified by the swollen abdomen .
 The males with their flat abdomen.
 A gentle hand pressing on the abdomen extrudes white milt from the male.
 Brood fish collection Brood fish were selected from two different ponds for
avoiding inbreeding.
 Weight of Thai koi female and male ranged from 65-100 g and 50-60 g
respectively.

6. Continued…
Breeding Set and Sex ratio :
 The sex ratio of female and male was kept at 1:2 and 1:1 in every breeding
set.
Hormone administration :
 After conditioning, the broods were taken out from the cistern, weighed and
amount of hormone required was calculated out.
 The alcohol preserved PGs were first dried with tissue paper; weighed and
homogenized with half the quantity of the distilled water.
 The supernatant solution was then drawn out in a syringe for injection.
 The injection was administered in the muscle of the base of pectoral fin.
 A single dose of PG hormone at 12 mg kg-1 for female .

7. Continued…
Fertilization, hatching and survival rates:
 After spawning, a sample of eggs taken in a Petri dish and the total number of
eggs and the number of fertilized eggs were carefully counted for
determining fertilization rate.
 Then the hatchlings came out through a range of time.
 Hatching rate calculated after a successive completion of hatching of each
breeding set.
 The larvae monitored for 3 days for the calculation of survival rate.
 After completion of the experiment, at 4th day, the number of total live
larvae in cistern counted separately for calculation of survival rate.

8. Continued…
Breeding of Thai koi :
 The PG extract was injected into the muscular basal part of the pectoral fin.
 Needle was inserted at an angle of 45° with the body.
 After injection, both the males and females were shifted to breeding tank
filled with 30 cm of water (10ft / 8ft) at 1‫1׃‬ ratio.
 Artificial aeration was provided continuously.
 The adults were removed from spawning tanks after fertilizing eggs and water
depth increased to 50 cm.
Data analysis :
 Data was collected carefully recorded and analyzed by using Microsoft Office
2007 version.

9. RESULT AND DISCUSSION:
 Criteria for identification of male and female broods of Thai koi:
Criteria Male Female
Size Small (average wt. 30-35 g) Large (average wt. 80-100 g)
Abdomen Normal Bulging and soft
Body shape Slender Robust
Pectoral fin Inner side rough Inner side smooth
Gentle pressure on
abdomen
Whitish milt come out through
the genital pore
Eggs come out through
the genital pore

10. BREEDING TECHNIQUE OF MAGUR
(Clarias batrachus)
Introduction
 Bangladesh is a land of high potential water resources. Fin
fish hatchery was first established in Jessore by Mohoshin
Master in 1967. Since then the number of fish hatchery has
increased uninterruptedly reaching over a thousand in 2010
to fulfill the ever increasing demand of the fin fish seeds for
aquaculture industry of Bangladesh.

11. Objectives:
 1. To know and practice the seed production technique of magur, shing and
pabda by using different types of hormones.
 2. To determine the effectives of different hormones for seed production of
magur
 3. To know the maintenance and hatchery operation technique.

12. MATERIALS AND METHODS
Seed production technique of magur (Clarias batrachus):
 Indigenous magur is a tasty and nutritious fish. In past magur was easily found
in natural waterbody. But day by day the abundance of this fish has been
decreased.
Brood fish rearing:
 Stocking density of magur was 50-100 broods per decimal.
 Magur become sexually mature at the age of 1yr when the weight is around
100g. 35% protein rich feed was used at the rate of 3% body weight.

13. Mature brood fish selection:
 Breeding season of magur is very short (June to July).
 During breeding season females were easily identified by their soft and
swollen abdomen due to presence of mature bulky eggs.
 On the other hand males are identified by their flat abdomen and long
protruded genital papillae.

14. Hormone extract preparation :
 Required amount of hormone was weighed by balance.
 Then ground by mortar and pestle manually with very small amount of water.
 Ground continuously until homogenous mixture was found.
 Maximum 1ml water was used per kg body weight of fish.
 Then the solution was taken into the syringe and kept into water to remain
cool.
 Hormone is prepared immediate before injection.

15. Hormone injection:
 PG (pituitary gland) extracts, HCG (Human Chorionic Gonadotropin) mixed
with PG and ovaprim mixed with PG solution were used as hormone to collect
the eggs. Only female was treated with hormone to collect eggs and for
collecting sperm untreated male was sacrificed.
Egg collection:
 Eggs were collected from the fish by stripping. Till now it was not possible to
induce the female to spawn naturally.
 For collection of milt, the testes were dissected out from the body cavity and
macerated in 0.9% salt solution.
 At first eggs were collected in a bowl from the female and at the same time
as soon as possible male was sacrificed to collect the sperm. To ensure the
maximum fertilization rate sperm suspension was mixed with eggs by gently
stirring with a feather in bowl (Fig. 3). The whole activities were finished
within 1- 1.5min.

16. Precautions of during seed production:
 1. Matured brood fish must be selected otherwise all activities will be
unsuccessful.
 2. During stripping gentle pressure should be put also.
 3. Egg density into the cistern should not be higher and they are carefully
kept into the cistern in such a way that the eggs are not attached together.
 4. Continuous water circulation should be ensured and 27-28oc water
temperature should be maintained.

17. Thank You