This document discusses induced breeding techniques for fish. It begins with an introduction to induced breeding, which is stimulating fish to breed artificially using pituitary hormones or synthetic hormones. It then covers the history of induced breeding, the role of the pituitary gland in releasing hormones that cause maturation and spawning, and techniques for extracting pituitary glands and injecting hormones. Factors that influence spawning and alternatives to pituitary extracts are also examined. The document concludes that hormone-induced spawning techniques can promote or inhibit the breeding process and provide a pure source of offspring.

1. JIWAJI UNIVERSITY
SOS IN ZOOLOGY
TOPIC:- INDUCED BREEDING
SUBMITTED TO:-
HEAD OF DEPARTMENT
SUBMITTED BY:-
HARDEEPAK SINGH
MSC 3 SEMESTER
rd

2. SYNOPSIS
• INTRODUCTION
• ITS HISTORY
• ITS IMPORTANCE
• FISH PITUITARY GLAND AND ITS ROLE
• TECHNIQUE AND ITS METHOD OF INJECTION
• FACTORS INFLUENCING THE SPAWNING
• SUBSTITUTES OF FISH PITUITARY GLANDS
• CONCLUSION
• REFERENCES

3. INTRODUCTION
Induced breeding is a technique by which the economically
important fish are breed through artificial stimulation.
Induced breeding is a technique whereby ripe fish breeders
are stimulated by pituitary hormone or any other synthetic
hormone to breed in captive condition.
The stimulation promotes timely release of sperms and eggs.
• It is also known as hypophysation

4. History of
Induced breeding
The technique of induced breeding was first
evolved in Argentina after producing pituitary
extract by Houssay 1930 where viviparous fish
was injected with the hormone to make
premature birth.
In India first attempt of induced breeding was
made by Hamid Khan in 1937 on Cirrhinus
mrigala.

5. Why Induced Breeding is Necessary ?
• It gives pure spawn of certain species of fishes under
cultivation.
• Spawn collected from natural water is not pure.
• It assures timely available of pure seed, It can fulfill
any quantity of demand in any time.
• The technique is very simple and does not need too
much technical assistance or knowledge.
• The cost of expenditure is very low than the natural
collections of spawns

6. Fish Pituitary Gland
Pituitary gland is an endocrine gland situated on the ventral side of the brain
• It is a small, soft, whitish body whose size and shape vary with species
• Pituitary is located in a concave cavity known as sella turcica and enclosed by a
thin membrane known as duramater
• It may be attached to the brain by a short stalk called the infundable stalk

7. Role of Pituitary Gland
• Pituitary glands secretes the
gonadotrophins hormones
Follicle stimulating hormone
(FSH) & Luteinizing hormone
(LH)
• FSH causes growth and
maturation of ovarian follicle
in females and
spermatogenensis in the
testis of males
• LH causes luteinization in
females and promote the
production of testosteron in
males

8. Induced Breeding
Technique
1. Removal of glands
i) through foramen magnum
ii) by dissecting head
2. Preservation of glands
3. Preparation of gland extract
4. Brooders selection
5. Injection to brooders
• I dose / Priliminary dosage
• II dose / Resolving dosage
6. Spawning

9. Methods For
Injecting Fish Brooder
1. Intra-muscular :- It is
administered into the muscle
on the caudal peduncle or
behind the dorsal fin, but above
the lateral line
2. Intra-peritoneal :- It is given
to the soft regions of the body,
generally at the base of the
pelvic fin or the pectoral fin

11. Factors Influencing
the Spawning of Fish
• Climate– 24°C to 31°C with cloudy days and rainy periods.
Light drizzling following heavy rains is ideal. In absence of
rain artificial showers are used.
• Water – Flowing water is preferred.
• Turbidity – 100ppm 1000ppm.
• Light – It is known to bring that light may help in early
maturation and spawning of fish

12. Substitutes of
fish pituitary gland
1.HCG (Organon) • In the case of silver carp, successful spawning
could be achieved by injecting HCG (Organon).
2. Synahorin • Synahorin along with carp pituitary gland was
successful in breeding rohu and silver carp.
3.Ovaprim • It is the new inducing hormone for fish and absolute
substitute of pituitary extract though it’s costly.

13. CONCLUSION
Hormone induced spawning techniques influence either promoting or inhibiting the
process. substances used for hormone induced spawning are pituitary extract,
Ovatide and Ovaprim etc to stimulate pituitary.
It gives pure spawn of certain species of fishes under cultivation, timely available of
pure seed, fulfil any quantity of demand in any time.

14. REFERENCES
https://www.researchgate.net/publication/261994048_Induced_Breeding
https://www.yourarticlelibrary.com/fish/applied-fisheries/induced-
breeding-of-fish-subject-matter-steps-and-advantages-fishes/88534
https://www.shcollege.ac.in/wp-
content/uploads/NAAC_Documents_IV_Cycle/Criterion-
II/2.3.2/ppt/Ms_LeenaRaphael_Inducedbreeding.pdf