how to make presentation for journal club

1. 1
Comparison of Electrospun and
Solvent Cast Polylactic Acid and
Polyvinyl Alcohol Inserts as
Potential Ocular Drug Delivery
Vehicles
Supervised By : Presented By :
Dr. Amit K. Goyal Jay k. Gupta
Professor M. Pharma (Pharmaceutics)
Department of pharmacy 2020MPP002
Department of Pharmacy
School of Chemical Sciences and Pharmacy
Central University of Rajasthan

2. 2
Impact factor – 7.328

3. Contents
1. Introduction
2. Objectives
3. Materials and Methods
4. Results and Discussion
5. Conclusion
6. References
3

4. Introduction
 Ocular inserts are sterile, thin,
multilayered, drug-impregnated, solid
or semisolid consistency devices
placed into the cul-de-sac or
conjuctival sac, whose size and shape
are especially designed for
ophthalmic application.
 Ocular inserts are better drug delivery
vehicles as compared to other forms.
 Ophthalmic inserts can be the choice
of dosage form, as they increase the
contact time and sustain the drug
release duration.
4

5. Objective
 To perform preformulation study of Dexamethsone and
polymers [poly-lactic acid (PLA) and poly vinyl
alcohol (PVA)].
 To fabricate Dexamethasone loaded inserts by
electrospining and solvent casting both techniques.
 In-vitro evaluation and characterization of ophthalmic
insert.
 To perform the comparative study of dexamethasone
insert prepared by electrospining and solvent casting
techniques.
5

6. 6
Materials Selection
S. No Materials
1 Dexamethasone
2 Polylactic acid
3 Polyvinyl Alcohol
4 N,N-Dimethylformamide
5 Chloroform

7. Method of Preparation
7
Fig. 1. Preparation of polymer solution

8. Method of Preparation
8
Fig. 2. Preparation of ophthalmic insert by solvent casting method

9. Method of Preparation
9
Fig. 3. Preparation of ophthalmic insert by electrospining method

10. 10
Fig. 4. (A) Electrospun nanofiber inserts, (B) solvent cast inserts.

11. Result and Discussion
 Dexamethasone-loaded inserts with drug concentrations of
1%, 5% and 10% were prepared using electrospinning and
solvent casting methods.
 The inserts obtained after electrospinning were
homogenous, smooth, uniform and white in color while
those obtained from solvent casting were rough, porous,
brittle and white in color with non homogeneous
appearance.
 ENIs were easily foldable and tend to stick to the surfaces
because of a static charge, while solvent casts were brittle
and uncharged.
 Inserts containing 50 μg of dexamethasone were cut and
used for the physicochemical characterization.
11
* ENI- electrospin nanofiber insert

12. In-vitro Drug Release Study
12
Fig. 5. In vitro release of dexamethasone from solvent cast inserts and electrospun
nanofiber inserts.

13. Scanning electron microscopy
13
Fig. 6. Scanning electron microscopy images of electrospun inserts of 1%
dexamethasone (A), 5% dexamethasone (B), 10% dexamethasone (C)

14. Scanning electron microscopy
14
Fig. 6. Scanning electron microscopy images of Solvent cast inserts of 1%
dexamethasone (D), 5% dexamethasone (E), 10% dexamethasone (F).

15. 15
Table. 1. Summary of film thickness, drug loading, drug content

16. X-ray diffraction
Fig.7. X-ray diffraction pattern of dexamethasone
16

17. 17
Fig. 8. X-ray diffraction pattern of electrospun nanofiber inserts (A),
solvent cast inserts (B)
X-ray diffraction

18. Intraocular pressure (IOP)
18
Fig. 9. Cell viability of electrospun blank (ENIB), electrospun formulation
(ENIF), solvent cast blank (SCIB) and solvent cast formulation (SCIF)
compared to negative control (Control) and positive control (DMSO).

19. Conclusion
 In the present study, researchers have prepared
dexamethasone-loaded PLA/ PVA inserts by
electrospinning and solvent casting methods.
 They characterized both inserts for suitability for ocular
application.
 Their results indicate that electrospun nanofibers inserts of
PLA/PVA perform better than inserts prepared by the
solvent casting technique and are capable of delivering
drugs in a sustained manner.
19

20. References
 Saettone, M. F.; Giannaccini, B.; Monti, D. Ophthalmic
emulsions and suspensions, J. Toxicol. Cutan. Ocul.
Toxicol. (2001), 20 (2–3), 183–201.
 Bucolo, ]. C.; Drago, F.; Salomone, S.; Ocular drug
delivery: a clue from nanotechnology, Front. Pharmacol.
(2012), 3, 188.
 Khairnar, A.; Jain, P.; Baviskar, D.; Jain, D.
Developmement of mucoadhesive buccal patch containing
aceclofenac: in vitro evaluations, Int. J. PharmTech Res.
(2009), 1 (4), 978–981.
 Smolin, G.; Okumoto, M.; Feiler, S.; Condon, D.
Idoxuridine-liposome therapy for herpes simplex keratitis,
Am J. Ophthalmol. (1981), 91 (2), 220–225. 20

21. 21