This document discusses developing a biosensor for detecting alcohol in foods and beverages. The objectives are to create a biosensor that uses enzymes to quickly and inexpensively test for alcohol contamination, which would help with halal certification of imported foods for Muslim communities. The proposed visual biosensor would detect alcohol by changing color. It aims to provide an easy-to-use and fast alternative to current expensive and complex analytical methods like gas chromatography. The experimental methodology would characterize the biosensor and test its ability to detect ethanol in real food and beverage samples while addressing interferences, reproducibility, and sensitivity.

1. Introduction
 Alcoholic drinks or beverages are prohibited in Islam and even a small amount of drink added into foods or drinks
will render the products Haram for Muslim users.
 Different methods are used for the quantification of the alcohol in food and beverages are high-performance liquid
chromatography-flame ionization, gas chromatography coupled to mass spectrometry, and Fourier transform near
infrared. However, these methods usually involve expensive equipment and complex sample preparation procedures.
 Biosensors are analytical devices to acquire specific quantitative data by the combination of biological element like
enzymes with a transducer.
 In this biosensor, for the stabilization of enzymes conducting polymer will be used. The visual biosensor will detect
the presence of ethanol by changing its color .
References
1.do Santos, R.B., et al., Flow thermal infrared enthalpimetry: Rapid and inexpensive determination of the alcohol content of distilled beverages. 2019. 200: p. 67-71.
2.Najiha, A.A., et al., A preliminary study on halal limits for ethanol content in food products. 2010. 6(1): p. 45-50

2. Problem Statement
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 The detection of ethanol in food and beverages by analytical methods such as
gas chromatography is very expensive and require tedious sample preparation
procedures.

3. Objectives
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1. The objective of the study is to develop a Biosensor for
alcohol detection to be used for food and beverages
contamination.
2. The Halal certification of imported food products must be
attained prior to consumption for muslim community through
quick test.
3. The use of enzymes as an alternate agent for the
determination of alcohol in beverages.

4. Novelty
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The visual biosensor will do the quick assessment of ethanol in drinks and foods.
It will be easy to use and will have a greater response time.

5. Overview of the Experimental Methodology
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Characterizations:
SEM
Image analysis software
GC analysis
Casting of enzyme onto polymer for immobilization
For coloured samples
Application in detecting ethanol in food and beverages
Preparation of conducting polymer
Real sample measurements
Interferences measurements
Reproducibility
Sensitivity