Achieve the bioproduction of microalgae and evaluate of bioactivity of compounds, with inmuno-stimulating activity, suitable for food fortification and the development of new food formats of specific functionality of strengthening the immune system

1. Enhancement of the immune system
from microalgae:
Bioproduction for novel green foods

2. 1. CONCEPT
GREEN FOODS GREEN SUPLEMENTS
• CONCEPT
• TECHNICAL
OBJECTIVES
• STRATEGIC
OBJECTIVES
• METODOLOGY AND MICROALGAE
ASSOCIATED WORK
Spirulina and Chlorella
PLAN
• CONSORTIUM
• IMPACT: NUMBERS
• RESULTS
• INMUGAL ‘S
PROJECT TEAM
NOVEL GREEN FOOD:
PROTOTYPES

3. 1. CONCEPT
Full Title: Strengthening of the immune system from
• CONCEPT
microalgae: Novel foods (green food)
• TECHNICAL
OBJECTIVES Acronym: INMUGAL
• STRATEGIC
OBJECTIVES Type of funding scheme:
• METODOLOGY AND
ASSOCIATED WORK The INMUGAL project is funded by the Spanish Ministry of
PLAN Industry Energy and Tourism (MINETUR), through the
• CONSORTIUM Profit program of technological centres for applied
• IMPACT: NUMBERS
research and Program projects for Experimental
• RESULTS
• INMUGAL ‘S
Development (Spanish PROFIT)
PROJECT TEAM
Budget:
It has a budget of 1.5 M€ and a duration of two years.
Consortium:
AINIA technology centre (leader)
+
AZTI - Tecnalia (partner)
LEIA - Tecnalia (partner)
INBIOTEC Centre (partner)

4. 2. TECHNICAL OBJECTIVES
• CONCEPT MAIN OBJECTIVE:
• TECHNICAL Achieve the bioproduction of microalgae and evaluate of
OBJECTIVES bioactivity of compounds, with inmuno-stimulating activity, suitable
• STRATEGIC for food fortification and the development of new food formats of
OBJECTIVES
specific functionality of strengthening the immune system.
• METODOLOGY AND
ASSOCIATED WORK
PLAN
TECHNICAL OBJECTIVES:
• CONSORTIUM
• IMPACT: NUMBERS
• RESULTS
1. Development of process technologies of bioproduction
• INMUGAL ‘S and cultivation of the microalgae.
PROJECT TEAM
2. Recovery and extraction techniques compounds/
extracts with potential functionalities.
3 Development and adjustment of technologies of suitable
microencapsulation of the compounds.
4. Identification of the bioactivity of fractions through the evaluation
of the inmunoestimulant properties.

5. 2. TECHNICAL OBJECTIVES
• CONCEPT
• TECHNICAL
OBJECTIVES 5. Microencapsulation of compounds obtained so as to ensure
• STRATEGIC their protection and controlled release.
OBJECTIVES
• METODOLOGY AND 6. Assessment of the bioavailability of the cells and the
ASSOCIATED WORK microcapsules through an automated Digestion Simulator.
Simulator
PLAN
• CONSORTIUM 7. Design formulated and food prototypes to evaluate the
• IMPACT: NUMBERS implementation in real product.
• RESULTS
• INMUGAL ‘S 8. Validation of the functionality in animal models (zebrafish
PROJECT TEAM embrios)
B C 9. Integration, scaling-up, yield calculations, balances of
A
the integrated process of bioproduction and recovery of
bioactive compounds.

6. 3. STRATEGIC OBJECTIVES
1. CONCEPT
2. TECHNICAL
OBJECTIVES
• STRATEGIC
DEVELOPMENT OF EXPERIMENTAL PLANS
OBJECTIVES
• METODOLOGY AND
ASSOCIATED WORK
PLAN INTEGRATION OF RESULTS
• CONSORTIUM OF THE CONSORTIUM
• IMPACT: NUMBERS
• RESULTS
• INMUGAL ‘S OBJECTIVE: DEVELOPMENT OF
PROJECT TEAM
PRODUCT
2012 GOAL:
DESIGN OF THE FIRST PROTOTYPES
-Food
-Feedingstuffs (farm/aquaculture)

7. 4. CONSORTIUM
1. CONCEPT
2. TECHNICAL 2. MICROALGAE STRAINS SELECTION. FOOD/LEGAL
APPLICATION CRITERIA
1. UPDATE THE STATE OF THE ART. MARKET APPROACH
OBJECTIVES
• STRATEGIC
8. COORDINATION AND TRANSFER OF RESULTS
OBJECTIVES
• CONSORTIUM
3. MICROALGAE INTEGRATED BIOPRODUCTION. MIXO-
• IMPACT: NUMBERS
HETEROTROPHIC CONDITIONS
• RESULTS
• INMUGAL ‘S
PROJECT TEAM
4. RECOVERY
5. BIOACTIVITY 6. MICROENCAPSULTION
EVALUATION
7. DEVELOPMENT OF NEW FOOD/FEED PROTOTYPES

8. 5. INPACT NUMBERS
1. CONCEPT
2. TECHNICAL
OBJECTIVES
• STRATEGIC
OBJECTIVES
BUDGET
• CONSORTIUM
Total: 1.577.411 €
• IMPACT: Funded: 1.183.058 €
NUMBERS (75%)
• RESULTS
• INMUGAL ‘S
WORKED HOURS
PROJECT TEAM
Total: 3.950 h
Experimental assays: 80%
Management: 20%
DIFFUSSION
Digital Press: > 500 IMPACTS
Scientific Papers: 10
TV-Radio: 15

9. 6. RESULTS
1. CONCEPT
Technical Specifications KNOW-HOW AND
2. TECHNICAL
and Prothocols of PROTOTYPES TRANSFERENCE
OBJECTIVES
Bioproduction, Extraction and
• STRATEGIC Microencapsulación of TO PRIVATE SECTOR
OBJECTIVES Compounds
• CONSORTIUM
• IMPACT: NUMBERS
Studies of Intestinal
• RESULTS
Absorption and
• INMUGAL ‘S
Bioavailability FOOD
PROJECT TEAM
PROTOTYPES
biscuits Low acid sauce

10. 7. INMUGAL’S PROJECT TEAM
1. CONCEPT
2. TECHNICAL
OBJECTIVES
• STRATEGIC
OBJECTIVES
• CONSORTIUM
• IMPACT: NUMBERS
• RESULTS
• INMUGAL ‘S
PROJECT TEAM
Consortium
Team

11. INMUGAL
Thank you for your attention
ainia, centro tecnológico
Parque tecnológico de Valencia
c/Benjamín Franklin 5-11
46980 Valencia (SPAIN)
http://www.ainia.es
Mercedes Villa-Carvajal. (INMUGAL COORDINATOR)
Villa-Carvajal
Biotechnology Researcher
Head of Bioproduction Pilot Plant
mvilla@ainia.es