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AHANA
AHANA
AHANA
ā€¢ Deposition of organism in
INFECTION     tissues & their growth
              resulting in HOST REACTION.


INFECTIVE   ā€¢ Number of organism required
  DOSE
AHANA         to cause an INFECTION.
AHANA
PATHOGENS




         AIR      BLOOD
        BORNE     BORNE

AHANA
AHANA
AHANA
AHANA
AHANA
AHANA
AIR BORNE

         DIRECT

        INDIRECT

 CROSS CONTAMINATION
AHANA
AIR BORNE CONTAMINATION




                           Spatter
                           (>50mm)
              Mists
Aerosols      (>/= 50mm)
(50į¹·m-50mm)
    AHANA
DIRECT                            INDIRECT
CONTAMINATION                      CONTAMINATION
ļ‚§   Direct contact with body   ļ‚§   Handling sterile
    fluids.                        equipments & areas with
                                   contaminated hands




      AHANA
CROSS
CONTAMINATION




PATIENT         DOCTOR


 AHANA
Elimination /
   reduction of
spread of infection
 from their hosts

   Removal of
 microorganisms
 from objects or
    surfaces
   AHANA
AHANA
STERILIZATION
IMMUNIZATION
               & DISINFECTION


 PERSONAL          DENTAL
  BARRIER        EQUIPMENT
 TECHNIQUE        ASEPSIS


  SURGICAL      PROCEDURAL
   ASEPSIS
  AHANA          BARRIERS
Vaccination
  plays an
important role

 Hepatitis B
  vaccine
  AHANA
Hand         Head
        washing        cap


        Eyewear      Facemask



        Protective
AHANA     gown       Gloves
AHANA
Primary
  source of
  infection
transmission
AHANA
HAND WASHINGā€¦ā€¦ā€¦..




   AHANA
HAND WASHINGā€¦ā€¦ā€¦..




 Mild antiseptics : 3% P-
 chloro, meta- xylenol /
 chlorhexidine
 Control pathogens & suppress
 overgrowth of skin bacteria
    AHANA
Head Cap ā€¦ā€¦ā€¦ā€¦


  Hair is       Hair
restrained   should be
   away         tied
from face     properly



   AHANA
Protective Eyewearā€¦ā€¦


Protection against foreign
bodies, splatter & aerosols


Protection against injury &
microbes


Do not touch with contaminated
hands
AHANA
Face masksā€¦ā€¦


          Should be changed regularly & b/w
                       patients
          Do not touch the mask with gloved
                        hands

            When wet should be changed

           To remove grasp only by strings
  AHANA
GOWNS




AHANA
GOWNSā€¦ā€¦ā€¦ā€¦.
 Protective over garments.

 Cotton or synthetic.

 Worn over dentistsā€™ clothes.

 Protection against spatter from routine work.


 Only in clinic & should be laundered daily.
   AHANA
GLOVES
                Prevents
           contamination of
            hands & control
             cross infection

              Should be
             changed btw
               patients

           Do not wash with
           detergents & thr
AHANA
             should be no
               defects.
GLOVES




  AHANA
Lowest possibility of manufacturing defects
Well fitting
Good tactile sensitivity
Do not cause hypersensitivity.
Conducive to glove powder.
Non offensive taste & odour.
Reasonable cost.
     AHANA
LATEX

HEAVY                STERILE
UTILITY   GLOVES
                    SURGICAL


          VINYL
            AHANA
High
           quality

Maximum
protection


          Expensive
  AHANA
Commonly
                      used in
                     dentistry

             Adversely affected
               by alcohols &
                detergents

Becomes tacky / sticky when
 AHANA     wet
Over gloves

         When intraoral
        procedures are to
         be interrupted

          Slipped over
         gloved hands.
AHANA
Wear resistant


NITRITE TYPE can be
washed, sterilized,
disinfected


Double gloves ā€“ Herpes, HIV, HBV


Boxes of gloves: stored away from sunlight in
tightly closed, heavy plastic bags__ minimize
     AHANA

oxidation
Surgical staff preparation


Surgical site preparation


 AHANA
         Draping the patient
Personal barrier protection



        Surgeon is helped into
           sterile gown by
         properly gowned &
           gloved assistant


AHANA
          Last step- wear gloves
SURGICAL SITE
       PREPARATION
Reduces postoperative infections.

Lubricating oil is applied patientā€™s eyes &
taped shut.

Removal of hair in surgical site

Whole area is scrubbed with iodophore
solution
Skin preparation should take only about
5 min.
    AHANA
DRAPING THE PATIENT

Isolate surgical area


Secured with towel
      clips

Waterproof drapes
  will be more
   beneficial
    AHANA
RUBBER DAM      Prevents aerosol
                formation of saliva

   SALIVA
                Minimize contact
  EJECTOR       with aerosols &
                spatter
                Rinsing mouth
ANTIMICROBIAL
  SOLUTION      reduces amount of
                bacteria
    AHANA
SALIVA   RUBBER   ANTIMICROBIAL
EJECTOR    DAM       SOLUTION



  AHANA
Clean & disinfect
    2
Approaches
              Prevention of
              contamination
   AHANA
DISPOSABLE, SINGLE-USE
INFECTION CONTROL BARRIER




  AHANA
PLASTIC
 FILM                 Al FOIL
 BAGS




            TREATED
    AHANA    FABRIC
DELIVERY SYSTEM
Surfaces should be cleaned & disinfected b/w each patient




Clean the surface by vigorous wiping, reapply the agent to disinfect
& keep the surface moist for 10 min.



Simple & most cost effective method is by use of single use barrier
materials
        AHANA
DENTAL CHAIR
        Should be smooth with
        minimum accessories

        Upholstery should be
        removable for cleaning

        Plastic sheets should
        cover switches

        Head rests & arm slings
AHANA
        should also be disinfected
OPERATOR STOOL

Covering the
lever with
plastic

Sheet material
should be of
vinyl plastic
  AHANA
DENTAL
        CABINETRY




AHANA
Materials & instruments should be
stored out of treatment room

              Cabinets should be made
              of materials that can be
              cleaned & disinfected
              regularly


                     Avoid organic
                     materials in
   AHANA
                     construction
UTILITY ITEMS
Supply of water, compressed air, suction etc.


   Excellent circulation of air with an exhaust to
   outside

     Prevent clogging of water passages by using
     water sediment filter & deionizing the incoming
     water

     Suction pumps should have smooth flow of water
     & air

   Sediment trap should be disassembled & cleaned
   regularly

The air compressor tank must be drained daily to avoid
 AHANA

microbial contamination
DENTAL UNIT WATER LINES




 AHANA
Dental unit water linesā€¦ā€¦.
           Bacterial biofilm is formed

              ADA - <200 CFU/ml

 Dental waterlines should be flushed at
       the beginning of the day.


 Chemical germicides should be used to
          flush the waterline

 Antiretraction valves can also be used
   AHANA
PRESOAKING             CLEANING



  PACKAGING            RINSE & DRY



          SEALING      STERILIZATION


            CLINICAL
  AHANA
                            STORAGE
              USE
Prevents debris from drying

Place instruments in a perforated
basket & then place it in holding
solution (neutral pH
detergents, water, Enzyme soln)

Do not soak for too long
  AHANA
PRESOAKINGā€¦ā€¦ā€¦ā€¦ā€¦




 AHANA
Reduces the
         bio burden


 MANUAL
SCRUBBING
                 ULTRASONIC
                  CLEANING
MECHANICAL
INSTRUMENT
  WASHER
AHANA
Removes debris but direct
contamination risks are high.
  AHANA
Produces billions of tiny
bubbles which further
collapses & create high
turbulence that
dislodges debris


Safer & more effective: 4
ā€“ 16 min

Discard the solution daily
     AHANA
Used mainly
        in hospitals


         Reduces
          direct
AHANA
         handling
Always keep the instruments dry.


Use hot air oven sterilization for
corrosion prone instruments


Spray rust inhibitor( sodium
nitrite)
AHANA
Maintains sterility of instruments

Self sealing, paper plastic & peel
pouches

Instruments will be kept wrapped
until used

Reduce the risk of contamination
   AHANA
PACKAGINGā€¦ā€¦..




AHANA
AHANA
Sunlight


             Flaming
PHYSICAL
           Incineration


 AHANA
           Hot air oven
Alcohols

            Aldehydes

             Halogens
CHEMICAL
              Phenols

            Metallic salt
              gases
  AHANA
           Surface active
              agents
ā€¢ Process by which an article ,
                  surface or medium is freed of all
STERILIZATION     microorganisms either in
                  vegetative or spore state

                ā€¢ Removal or destruction of
DISINFECTION      any microorganism capable
                  of causing infection
                ā€¢ Measure used to prevent
ANTISEPSIS/       infection by inhibiting
  ASEPSIS
    AHANA         growth of microbes on living
                  surfaces
MOIST
             DRY HEAT
   HEAT

         METHODS

CHEMICAL
             ETHYLENE
 VAPOUR
               OXIDE
PRESSURE
 AHANA
AHANA
AUTOCLAVEā€¦ā€¦ā€¦
ļ‚§ Most efficient & reliable method.
ļ‚§ 3 main phases;

                   ā€¢ Vacuum created followed by
Pretreatment         induction of steam
   Phase           ā€¢ Saturated steam affects all
                     instruments

                   ā€¢ Temperature rises to peak
Sterilization
                     point
   Phase
                   ā€¢ Holding time

    Post
                   ā€¢ Steam removed by vacuum
 treatment
    AHANA
                   ā€¢ Ensure instruments are dried
   Phase
TEMPERATURE           TIME

    121ĖšC        15 minutes
    126ĖšC        10 minutes
    134ĖšC        3 minutes
         15 lbs PRESSURE
 AHANA
ADVANTAGES
Time efficient


Good penetration

Consistently good & reliable
results

Instruments can be wrapped
prior to sterilization
AHANA
DISADVANTAGES



         Blunting & corrosion
         of sharp instruments
          Damage to rubber
              goods
 AHANA
AHANA
Hot air ovenā€¦ā€¦.
Utilizes radiating heat: long periods of exposure

                Packaging materials
   Paper &        Wrapped               Nylon plastic
                              Al foil
 plastic bags     cassettes                tubing




      AHANA
TEMPERATURE     TIME
   141ĖšC          3hr
   149ĖšC         2.5hr
   160ĖšC          2hr
   170ĖšC          1 hr
   180ĖšC      30 minutes
  AHANA
ADVANTAGES
No corrosion & blunting


Low cost of equipment


Instruments are dry after cycle

Rapid cycles are possible at
higher temperature
AHANA
DISADVANTAGES
             Poor penetrating capacity

  High temperature may damage heat
           sensitive items

Inaccurate calibration & lack of attention
    to proper settings lead to errors

            Not suitable for hand pieces


               May discolour fabrics
    AHANA
AHANA
Chemical vapour sterilizationā€¦ā€¦




             HEATED IN
CHEMICAL                      HOT
              CLOSED        CHEMICAL
SOLUTION
             CHAMBER        VAPOURS


               Pressure
                          Kill microbes


    AHANA
ACTIVE INGREDIENTS 0.23%
                   Formaldehyde
OTHERS             72.38 % Ethanol +
                   Acetone + water +
                   Other Alcohols


   AHANA
M
O
D
E
S
            COAGULATION OF    CELL MEMBRANE
O              PROTEIN         DISRUPTION

F
A
C
T
I
O           REMOVAL OF FREE
                               SUBSTRATE
N             SULFHYDRYL
    AHANA
                              COMPETITION
                GROUP
TEMPERATURE   PRESSURE   TIME


  132ĖšC         20lb     30min
   AHANA
Eliminates corrosion of carbon
steel instruments, burs & pliers

  AHANA
DISADVANTAGES

         Items sensitive to elevated
          temperature are damaged

Sterilization of liner, textiles, fabric or
   paper towels not recommended

    Instruments should be dry when
 AHANA          loaded
AHANA
ETOXā€¦ā€¦ā€¦ā€¦..
    Best method for sterilizing complex
              instruments

 ETOX is highly penetrative, noncorrosive
            gas above 10.8ĖšC

  Cidal action against bacteria, spores &
                 viruses.

 Suited for electric equipments, flexible
    fiber endoscopes & photographic
 AHANA          equipment
ADVANTAGES
             Leaves no residue

                 Deodorizer

              Good penetration

               Suited for heat
              sensitive articles
AHANA
DISADVANTAGES
               High cost


             Toxic, irritant,
               mutagenic,
              carcinogenic

           Explosive & highly
             inflammable
AHANA
AHANA
AHANA
BOILING WATERā€¦ā€¦ā€¦ā€¦ā€¦..

100ĖšC at normal atmospheric
pressure


10 min exposure kills almost all
bacteria & some viruses


Not suggested for tissue
penetrating instruments.
AHANA
AHANA
Glass bead sterilizerā€¦..
 Rapid , chair side method of sterilization

 Usually uses table salt which consists of ~ 1 %
 sodium silico aluminate & sodium carbonate

 Salt can be replaced by glass beads if < 1 mm in
 diameter because , larger beads are not efficient
 in transferring heat to endodontic instruments


 5- 15 sec at 260Ā° C
   AHANA
AHANA
IRRADIATIONā€¦.
2 TYPES

              IONIZING
            NON IONIZING

    AHANA
X rays, Gamma rays, High
     speed electrons


   Effective- heat labile
items; needles, syringes,
  culture tray, cannulaā€¦


High energy gamma rays
     from Cobalt 60
    AHANA
UV LIGHT
        ā€¢ Absorbed by proteins & nucleic
          acids & kill microbes
        ā€¢ Purification of air



                  INFRA RED
        ā€¢ To sterilize large number of
          syringes sealed in metal container.
AHANA
        ā€¢ Has no penetrating ability
AUTOCLAVE TAPE    THERMOCOUPLE




                            CHEMICAL
AHANA
        MICROBIAL TESTS
                           INDICATORS
CRITICAL            SEMI- CRITICAL NON CRITICAL

Curettes, burs,     Amalgam           Light switches,
files, scalpels     condensers,       counter tops,
                    mouth mirror,     drawer pulls or
                    saliva ejectors   cabinets
ā€¢Touch sterile      ā€¢ Touch mucous    ā€¢Do not come in
areas of body /     membrane          contact with oral
penetrate tissues   without           mucosa
& enter vascular    penetrating       ā€¢Touched hands
system              tissues.          contaminated
ā€¢Sterilized &       ā€¢Should be        with saliva/ blood
stored in
       AHANA        sterilized /
packages            disinfected.
INSTRUMENT         STERILIZATION
  CATEGORY             METHOD
   CRITICAL     HEAT STERILIZED & Stored
                in packages


SEMI CRITICAL   HEAT STERILIZED / Treated
                with high level disinfectants
                after cleaning


NON CRITICAL    Treated with intermediate to
                low disinfectant after
                cleaning
AHANA
Constant contact with oral fluids result in
formation of biofilm within the handpiece.


This holds potential to infect a healthy patient.


Tween 80 & Ponceau 4R


3% hydrogen peroxide


But cannot replace autoclaving.
     AHANA
Handpiece sterilizationā€¦ā€¦
Autoclave sterilization

Proper cleaning & lubricating
procedures should be done

Cleaning with disinfectants

Chemical vapour pressure & ETOX
sterilization can be done
  AHANA
STERILIZATION
       OF BURS
             Critical item

Autoclaving can be done but may
        result in rusting.

          ETOX sterilization.
  AHANA
INFECTION CONTROL
  FOR IMPRESSIONS
Thoroughly wash under running
water


2% gluteraldehyde for 10 min


Alginate impressions ā€“ Spray an
iodophor


Store items in separate sealed bags.
 AHANA
DISINFECTION
  LEVEL         COMPOUND
    LOW        ETHANOL &        ā€¢ Antibacterial activity
               ISOPROPYL        ā€¢ Denature proteins
               ALCOHOLS         ā€¢ Maximum 10 min
                                  contact
INTERMEDIATE   PHENOLS          ā€¢ In high concentration
                                  protoplasmic poison
                                ā€¢ Precipitate proteins &
                                  destroy cell wall

   HIGH        FORMALDEHYDE &   ā€¢ Toxic & irritant
               GLUTERALDEHYDE   ā€¢ Active against most
                                  vegetative forms &
                                  spores
                                ā€¢ 2 % soln requires
                                  20min for disinfection &
     AHANA
                                  6 to 10 hrs for
                                  sterilization.
AHANA
1.   Employers must provide HB immunization to employees
     without charge within 10 days of employment.
2.   Universal precautions should be observed to prevent contact
     with blood & other potentially infectious materials.
3.   Employers must implement control systems to reduce
     production of contaminated spatter, aerosols etc.
4.   Safe handling of needles & other sharps.
5.   Disposal of single use needles, wires , sharps etc. in hard
     walled, leak proof containers from which needles cant be
     easily spilled.
6.   Proper hand washing
7.   Providing proper personal protective barriers to employees.
8.   Proper sterilization & disinfection system
9.   Regular laundry services should be arranged.
 AHANA
BIO
             HAZARDOUS
               WASTE
BIOMEDICAL
  WASTE
              MEDICAL
               SOLID
               WASTE
  AHANA
BIO HAZARDOUS
      WASTE

           Laboratory waste

          Fluid blood elements

                Sharps

Human body parts, tissues etc.
  AHANA
Empty specimen containers


Bandages & dressings


Surgical gloves

Decontaminated bio hazardous
wastes.
 AHANA
DISPOSAL BAGS




AHANA
DISPOSAL METHODS




 AHANA
AHANA
AHANA
AHANA
AHANA

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INFECTION CONTROL Ahana A.

  • 4. ā€¢ Deposition of organism in INFECTION tissues & their growth resulting in HOST REACTION. INFECTIVE ā€¢ Number of organism required DOSE AHANA to cause an INFECTION.
  • 6. PATHOGENS AIR BLOOD BORNE BORNE AHANA
  • 10. AHANA
  • 11. AHANA
  • 12. AIR BORNE DIRECT INDIRECT CROSS CONTAMINATION AHANA
  • 13. AIR BORNE CONTAMINATION Spatter (>50mm) Mists Aerosols (>/= 50mm) (50į¹·m-50mm) AHANA
  • 14. DIRECT INDIRECT CONTAMINATION CONTAMINATION ļ‚§ Direct contact with body ļ‚§ Handling sterile fluids. equipments & areas with contaminated hands AHANA
  • 16. Elimination / reduction of spread of infection from their hosts Removal of microorganisms from objects or surfaces AHANA
  • 17. AHANA
  • 18. STERILIZATION IMMUNIZATION & DISINFECTION PERSONAL DENTAL BARRIER EQUIPMENT TECHNIQUE ASEPSIS SURGICAL PROCEDURAL ASEPSIS AHANA BARRIERS
  • 19. Vaccination plays an important role Hepatitis B vaccine AHANA
  • 20. Hand Head washing cap Eyewear Facemask Protective AHANA gown Gloves
  • 21. AHANA
  • 22. Primary source of infection transmission AHANA
  • 24. HAND WASHINGā€¦ā€¦ā€¦.. Mild antiseptics : 3% P- chloro, meta- xylenol / chlorhexidine Control pathogens & suppress overgrowth of skin bacteria AHANA
  • 25. Head Cap ā€¦ā€¦ā€¦ā€¦ Hair is Hair restrained should be away tied from face properly AHANA
  • 26. Protective Eyewearā€¦ā€¦ Protection against foreign bodies, splatter & aerosols Protection against injury & microbes Do not touch with contaminated hands AHANA
  • 27. Face masksā€¦ā€¦ Should be changed regularly & b/w patients Do not touch the mask with gloved hands When wet should be changed To remove grasp only by strings AHANA
  • 29. GOWNSā€¦ā€¦ā€¦ā€¦. Protective over garments. Cotton or synthetic. Worn over dentistsā€™ clothes. Protection against spatter from routine work. Only in clinic & should be laundered daily. AHANA
  • 30. GLOVES Prevents contamination of hands & control cross infection Should be changed btw patients Do not wash with detergents & thr AHANA should be no defects.
  • 32. Lowest possibility of manufacturing defects Well fitting Good tactile sensitivity Do not cause hypersensitivity. Conducive to glove powder. Non offensive taste & odour. Reasonable cost. AHANA
  • 33. LATEX HEAVY STERILE UTILITY GLOVES SURGICAL VINYL AHANA
  • 34. High quality Maximum protection Expensive AHANA
  • 35. Commonly used in dentistry Adversely affected by alcohols & detergents Becomes tacky / sticky when AHANA wet
  • 36. Over gloves When intraoral procedures are to be interrupted Slipped over gloved hands. AHANA
  • 37. Wear resistant NITRITE TYPE can be washed, sterilized, disinfected Double gloves ā€“ Herpes, HIV, HBV Boxes of gloves: stored away from sunlight in tightly closed, heavy plastic bags__ minimize AHANA oxidation
  • 38. Surgical staff preparation Surgical site preparation AHANA Draping the patient
  • 39. Personal barrier protection Surgeon is helped into sterile gown by properly gowned & gloved assistant AHANA Last step- wear gloves
  • 40. SURGICAL SITE PREPARATION Reduces postoperative infections. Lubricating oil is applied patientā€™s eyes & taped shut. Removal of hair in surgical site Whole area is scrubbed with iodophore solution Skin preparation should take only about 5 min. AHANA
  • 41. DRAPING THE PATIENT Isolate surgical area Secured with towel clips Waterproof drapes will be more beneficial AHANA
  • 42. RUBBER DAM Prevents aerosol formation of saliva SALIVA Minimize contact EJECTOR with aerosols & spatter Rinsing mouth ANTIMICROBIAL SOLUTION reduces amount of bacteria AHANA
  • 43. SALIVA RUBBER ANTIMICROBIAL EJECTOR DAM SOLUTION AHANA
  • 44. Clean & disinfect 2 Approaches Prevention of contamination AHANA
  • 46. PLASTIC FILM Al FOIL BAGS TREATED AHANA FABRIC
  • 47. DELIVERY SYSTEM Surfaces should be cleaned & disinfected b/w each patient Clean the surface by vigorous wiping, reapply the agent to disinfect & keep the surface moist for 10 min. Simple & most cost effective method is by use of single use barrier materials AHANA
  • 48. DENTAL CHAIR Should be smooth with minimum accessories Upholstery should be removable for cleaning Plastic sheets should cover switches Head rests & arm slings AHANA should also be disinfected
  • 49. OPERATOR STOOL Covering the lever with plastic Sheet material should be of vinyl plastic AHANA
  • 50. DENTAL CABINETRY AHANA
  • 51. Materials & instruments should be stored out of treatment room Cabinets should be made of materials that can be cleaned & disinfected regularly Avoid organic materials in AHANA construction
  • 52. UTILITY ITEMS Supply of water, compressed air, suction etc. Excellent circulation of air with an exhaust to outside Prevent clogging of water passages by using water sediment filter & deionizing the incoming water Suction pumps should have smooth flow of water & air Sediment trap should be disassembled & cleaned regularly The air compressor tank must be drained daily to avoid AHANA microbial contamination
  • 53. DENTAL UNIT WATER LINES AHANA
  • 54. Dental unit water linesā€¦ā€¦. Bacterial biofilm is formed ADA - <200 CFU/ml Dental waterlines should be flushed at the beginning of the day. Chemical germicides should be used to flush the waterline Antiretraction valves can also be used AHANA
  • 55. PRESOAKING CLEANING PACKAGING RINSE & DRY SEALING STERILIZATION CLINICAL AHANA STORAGE USE
  • 56. Prevents debris from drying Place instruments in a perforated basket & then place it in holding solution (neutral pH detergents, water, Enzyme soln) Do not soak for too long AHANA
  • 58. Reduces the bio burden MANUAL SCRUBBING ULTRASONIC CLEANING MECHANICAL INSTRUMENT WASHER AHANA
  • 59. Removes debris but direct contamination risks are high. AHANA
  • 60. Produces billions of tiny bubbles which further collapses & create high turbulence that dislodges debris Safer & more effective: 4 ā€“ 16 min Discard the solution daily AHANA
  • 61. Used mainly in hospitals Reduces direct AHANA handling
  • 62. Always keep the instruments dry. Use hot air oven sterilization for corrosion prone instruments Spray rust inhibitor( sodium nitrite) AHANA
  • 63. Maintains sterility of instruments Self sealing, paper plastic & peel pouches Instruments will be kept wrapped until used Reduce the risk of contamination AHANA
  • 65. AHANA
  • 66. Sunlight Flaming PHYSICAL Incineration AHANA Hot air oven
  • 67. Alcohols Aldehydes Halogens CHEMICAL Phenols Metallic salt gases AHANA Surface active agents
  • 68. ā€¢ Process by which an article , surface or medium is freed of all STERILIZATION microorganisms either in vegetative or spore state ā€¢ Removal or destruction of DISINFECTION any microorganism capable of causing infection ā€¢ Measure used to prevent ANTISEPSIS/ infection by inhibiting ASEPSIS AHANA growth of microbes on living surfaces
  • 69. MOIST DRY HEAT HEAT METHODS CHEMICAL ETHYLENE VAPOUR OXIDE PRESSURE AHANA
  • 70. AHANA
  • 71. AUTOCLAVEā€¦ā€¦ā€¦ ļ‚§ Most efficient & reliable method. ļ‚§ 3 main phases; ā€¢ Vacuum created followed by Pretreatment induction of steam Phase ā€¢ Saturated steam affects all instruments ā€¢ Temperature rises to peak Sterilization point Phase ā€¢ Holding time Post ā€¢ Steam removed by vacuum treatment AHANA ā€¢ Ensure instruments are dried Phase
  • 72. TEMPERATURE TIME 121ĖšC 15 minutes 126ĖšC 10 minutes 134ĖšC 3 minutes 15 lbs PRESSURE AHANA
  • 73. ADVANTAGES Time efficient Good penetration Consistently good & reliable results Instruments can be wrapped prior to sterilization AHANA
  • 74. DISADVANTAGES Blunting & corrosion of sharp instruments Damage to rubber goods AHANA
  • 75. AHANA
  • 76. Hot air ovenā€¦ā€¦. Utilizes radiating heat: long periods of exposure Packaging materials Paper & Wrapped Nylon plastic Al foil plastic bags cassettes tubing AHANA
  • 77. TEMPERATURE TIME 141ĖšC 3hr 149ĖšC 2.5hr 160ĖšC 2hr 170ĖšC 1 hr 180ĖšC 30 minutes AHANA
  • 78. ADVANTAGES No corrosion & blunting Low cost of equipment Instruments are dry after cycle Rapid cycles are possible at higher temperature AHANA
  • 79. DISADVANTAGES Poor penetrating capacity High temperature may damage heat sensitive items Inaccurate calibration & lack of attention to proper settings lead to errors Not suitable for hand pieces May discolour fabrics AHANA
  • 80. AHANA
  • 81. Chemical vapour sterilizationā€¦ā€¦ HEATED IN CHEMICAL HOT CLOSED CHEMICAL SOLUTION CHAMBER VAPOURS Pressure Kill microbes AHANA
  • 82. ACTIVE INGREDIENTS 0.23% Formaldehyde OTHERS 72.38 % Ethanol + Acetone + water + Other Alcohols AHANA
  • 83. M O D E S COAGULATION OF CELL MEMBRANE O PROTEIN DISRUPTION F A C T I O REMOVAL OF FREE SUBSTRATE N SULFHYDRYL AHANA COMPETITION GROUP
  • 84. TEMPERATURE PRESSURE TIME 132ĖšC 20lb 30min AHANA
  • 85. Eliminates corrosion of carbon steel instruments, burs & pliers AHANA
  • 86. DISADVANTAGES Items sensitive to elevated temperature are damaged Sterilization of liner, textiles, fabric or paper towels not recommended Instruments should be dry when AHANA loaded
  • 87. AHANA
  • 88. ETOXā€¦ā€¦ā€¦ā€¦.. Best method for sterilizing complex instruments ETOX is highly penetrative, noncorrosive gas above 10.8ĖšC Cidal action against bacteria, spores & viruses. Suited for electric equipments, flexible fiber endoscopes & photographic AHANA equipment
  • 89. ADVANTAGES Leaves no residue Deodorizer Good penetration Suited for heat sensitive articles AHANA
  • 90. DISADVANTAGES High cost Toxic, irritant, mutagenic, carcinogenic Explosive & highly inflammable AHANA
  • 91. AHANA
  • 92. AHANA
  • 93. BOILING WATERā€¦ā€¦ā€¦ā€¦ā€¦.. 100ĖšC at normal atmospheric pressure 10 min exposure kills almost all bacteria & some viruses Not suggested for tissue penetrating instruments. AHANA
  • 94. AHANA
  • 95. Glass bead sterilizerā€¦.. Rapid , chair side method of sterilization Usually uses table salt which consists of ~ 1 % sodium silico aluminate & sodium carbonate Salt can be replaced by glass beads if < 1 mm in diameter because , larger beads are not efficient in transferring heat to endodontic instruments 5- 15 sec at 260Ā° C AHANA
  • 96. AHANA
  • 97. IRRADIATIONā€¦. 2 TYPES IONIZING NON IONIZING AHANA
  • 98. X rays, Gamma rays, High speed electrons Effective- heat labile items; needles, syringes, culture tray, cannulaā€¦ High energy gamma rays from Cobalt 60 AHANA
  • 99. UV LIGHT ā€¢ Absorbed by proteins & nucleic acids & kill microbes ā€¢ Purification of air INFRA RED ā€¢ To sterilize large number of syringes sealed in metal container. AHANA ā€¢ Has no penetrating ability
  • 100. AUTOCLAVE TAPE THERMOCOUPLE CHEMICAL AHANA MICROBIAL TESTS INDICATORS
  • 101. CRITICAL SEMI- CRITICAL NON CRITICAL Curettes, burs, Amalgam Light switches, files, scalpels condensers, counter tops, mouth mirror, drawer pulls or saliva ejectors cabinets ā€¢Touch sterile ā€¢ Touch mucous ā€¢Do not come in areas of body / membrane contact with oral penetrate tissues without mucosa & enter vascular penetrating ā€¢Touched hands system tissues. contaminated ā€¢Sterilized & ā€¢Should be with saliva/ blood stored in AHANA sterilized / packages disinfected.
  • 102. INSTRUMENT STERILIZATION CATEGORY METHOD CRITICAL HEAT STERILIZED & Stored in packages SEMI CRITICAL HEAT STERILIZED / Treated with high level disinfectants after cleaning NON CRITICAL Treated with intermediate to low disinfectant after cleaning AHANA
  • 103. Constant contact with oral fluids result in formation of biofilm within the handpiece. This holds potential to infect a healthy patient. Tween 80 & Ponceau 4R 3% hydrogen peroxide But cannot replace autoclaving. AHANA
  • 104. Handpiece sterilizationā€¦ā€¦ Autoclave sterilization Proper cleaning & lubricating procedures should be done Cleaning with disinfectants Chemical vapour pressure & ETOX sterilization can be done AHANA
  • 105. STERILIZATION OF BURS Critical item Autoclaving can be done but may result in rusting. ETOX sterilization. AHANA
  • 106. INFECTION CONTROL FOR IMPRESSIONS Thoroughly wash under running water 2% gluteraldehyde for 10 min Alginate impressions ā€“ Spray an iodophor Store items in separate sealed bags. AHANA
  • 107. DISINFECTION LEVEL COMPOUND LOW ETHANOL & ā€¢ Antibacterial activity ISOPROPYL ā€¢ Denature proteins ALCOHOLS ā€¢ Maximum 10 min contact INTERMEDIATE PHENOLS ā€¢ In high concentration protoplasmic poison ā€¢ Precipitate proteins & destroy cell wall HIGH FORMALDEHYDE & ā€¢ Toxic & irritant GLUTERALDEHYDE ā€¢ Active against most vegetative forms & spores ā€¢ 2 % soln requires 20min for disinfection & AHANA 6 to 10 hrs for sterilization.
  • 108. AHANA
  • 109. 1. Employers must provide HB immunization to employees without charge within 10 days of employment. 2. Universal precautions should be observed to prevent contact with blood & other potentially infectious materials. 3. Employers must implement control systems to reduce production of contaminated spatter, aerosols etc. 4. Safe handling of needles & other sharps. 5. Disposal of single use needles, wires , sharps etc. in hard walled, leak proof containers from which needles cant be easily spilled. 6. Proper hand washing 7. Providing proper personal protective barriers to employees. 8. Proper sterilization & disinfection system 9. Regular laundry services should be arranged. AHANA
  • 110. BIO HAZARDOUS WASTE BIOMEDICAL WASTE MEDICAL SOLID WASTE AHANA
  • 111. BIO HAZARDOUS WASTE Laboratory waste Fluid blood elements Sharps Human body parts, tissues etc. AHANA
  • 112. Empty specimen containers Bandages & dressings Surgical gloves Decontaminated bio hazardous wastes. AHANA
  • 115. AHANA
  • 116. AHANA
  • 117. AHANA
  • 118. AHANA