4. ā¢ Deposition of organism in
INFECTION tissues & their growth
resulting in HOST REACTION.
INFECTIVE ā¢ Number of organism required
DOSE
AHANA to cause an INFECTION.
14. DIRECT INDIRECT
CONTAMINATION CONTAMINATION
ļ§ Direct contact with body ļ§ Handling sterile
fluids. equipments & areas with
contaminated hands
AHANA
27. Face masksā¦ā¦
Should be changed regularly & b/w
patients
Do not touch the mask with gloved
hands
When wet should be changed
To remove grasp only by strings
AHANA
29. GOWNSā¦ā¦ā¦ā¦.
Protective over garments.
Cotton or synthetic.
Worn over dentistsā clothes.
Protection against spatter from routine work.
Only in clinic & should be laundered daily.
AHANA
30. GLOVES
Prevents
contamination of
hands & control
cross infection
Should be
changed btw
patients
Do not wash with
detergents & thr
AHANA
should be no
defects.
32. Lowest possibility of manufacturing defects
Well fitting
Good tactile sensitivity
Do not cause hypersensitivity.
Conducive to glove powder.
Non offensive taste & odour.
Reasonable cost.
AHANA
33. LATEX
HEAVY STERILE
UTILITY GLOVES
SURGICAL
VINYL
AHANA
34. High
quality
Maximum
protection
Expensive
AHANA
35. Commonly
used in
dentistry
Adversely affected
by alcohols &
detergents
Becomes tacky / sticky when
AHANA wet
36. Over gloves
When intraoral
procedures are to
be interrupted
Slipped over
gloved hands.
AHANA
37. Wear resistant
NITRITE TYPE can be
washed, sterilized,
disinfected
Double gloves ā Herpes, HIV, HBV
Boxes of gloves: stored away from sunlight in
tightly closed, heavy plastic bags__ minimize
AHANA
oxidation
39. Personal barrier protection
Surgeon is helped into
sterile gown by
properly gowned &
gloved assistant
AHANA
Last step- wear gloves
40. SURGICAL SITE
PREPARATION
Reduces postoperative infections.
Lubricating oil is applied patientās eyes &
taped shut.
Removal of hair in surgical site
Whole area is scrubbed with iodophore
solution
Skin preparation should take only about
5 min.
AHANA
41. DRAPING THE PATIENT
Isolate surgical area
Secured with towel
clips
Waterproof drapes
will be more
beneficial
AHANA
42. RUBBER DAM Prevents aerosol
formation of saliva
SALIVA
Minimize contact
EJECTOR with aerosols &
spatter
Rinsing mouth
ANTIMICROBIAL
SOLUTION reduces amount of
bacteria
AHANA
43. SALIVA RUBBER ANTIMICROBIAL
EJECTOR DAM SOLUTION
AHANA
47. DELIVERY SYSTEM
Surfaces should be cleaned & disinfected b/w each patient
Clean the surface by vigorous wiping, reapply the agent to disinfect
& keep the surface moist for 10 min.
Simple & most cost effective method is by use of single use barrier
materials
AHANA
48. DENTAL CHAIR
Should be smooth with
minimum accessories
Upholstery should be
removable for cleaning
Plastic sheets should
cover switches
Head rests & arm slings
AHANA
should also be disinfected
51. Materials & instruments should be
stored out of treatment room
Cabinets should be made
of materials that can be
cleaned & disinfected
regularly
Avoid organic
materials in
AHANA
construction
52. UTILITY ITEMS
Supply of water, compressed air, suction etc.
Excellent circulation of air with an exhaust to
outside
Prevent clogging of water passages by using
water sediment filter & deionizing the incoming
water
Suction pumps should have smooth flow of water
& air
Sediment trap should be disassembled & cleaned
regularly
The air compressor tank must be drained daily to avoid
AHANA
microbial contamination
54. Dental unit water linesā¦ā¦.
Bacterial biofilm is formed
ADA - <200 CFU/ml
Dental waterlines should be flushed at
the beginning of the day.
Chemical germicides should be used to
flush the waterline
Antiretraction valves can also be used
AHANA
56. Prevents debris from drying
Place instruments in a perforated
basket & then place it in holding
solution (neutral pH
detergents, water, Enzyme soln)
Do not soak for too long
AHANA
60. Produces billions of tiny
bubbles which further
collapses & create high
turbulence that
dislodges debris
Safer & more effective: 4
ā 16 min
Discard the solution daily
AHANA
61. Used mainly
in hospitals
Reduces
direct
AHANA
handling
62. Always keep the instruments dry.
Use hot air oven sterilization for
corrosion prone instruments
Spray rust inhibitor( sodium
nitrite)
AHANA
63. Maintains sterility of instruments
Self sealing, paper plastic & peel
pouches
Instruments will be kept wrapped
until used
Reduce the risk of contamination
AHANA
66. Sunlight
Flaming
PHYSICAL
Incineration
AHANA
Hot air oven
67. Alcohols
Aldehydes
Halogens
CHEMICAL
Phenols
Metallic salt
gases
AHANA
Surface active
agents
68. ā¢ Process by which an article ,
surface or medium is freed of all
STERILIZATION microorganisms either in
vegetative or spore state
ā¢ Removal or destruction of
DISINFECTION any microorganism capable
of causing infection
ā¢ Measure used to prevent
ANTISEPSIS/ infection by inhibiting
ASEPSIS
AHANA growth of microbes on living
surfaces
71. AUTOCLAVEā¦ā¦ā¦
ļ§ Most efficient & reliable method.
ļ§ 3 main phases;
ā¢ Vacuum created followed by
Pretreatment induction of steam
Phase ā¢ Saturated steam affects all
instruments
ā¢ Temperature rises to peak
Sterilization
point
Phase
ā¢ Holding time
Post
ā¢ Steam removed by vacuum
treatment
AHANA
ā¢ Ensure instruments are dried
Phase
72. TEMPERATURE TIME
121ĖC 15 minutes
126ĖC 10 minutes
134ĖC 3 minutes
15 lbs PRESSURE
AHANA
76. Hot air ovenā¦ā¦.
Utilizes radiating heat: long periods of exposure
Packaging materials
Paper & Wrapped Nylon plastic
Al foil
plastic bags cassettes tubing
AHANA
77. TEMPERATURE TIME
141ĖC 3hr
149ĖC 2.5hr
160ĖC 2hr
170ĖC 1 hr
180ĖC 30 minutes
AHANA
78. ADVANTAGES
No corrosion & blunting
Low cost of equipment
Instruments are dry after cycle
Rapid cycles are possible at
higher temperature
AHANA
79. DISADVANTAGES
Poor penetrating capacity
High temperature may damage heat
sensitive items
Inaccurate calibration & lack of attention
to proper settings lead to errors
Not suitable for hand pieces
May discolour fabrics
AHANA
86. DISADVANTAGES
Items sensitive to elevated
temperature are damaged
Sterilization of liner, textiles, fabric or
paper towels not recommended
Instruments should be dry when
AHANA loaded
88. ETOXā¦ā¦ā¦ā¦..
Best method for sterilizing complex
instruments
ETOX is highly penetrative, noncorrosive
gas above 10.8ĖC
Cidal action against bacteria, spores &
viruses.
Suited for electric equipments, flexible
fiber endoscopes & photographic
AHANA equipment
89. ADVANTAGES
Leaves no residue
Deodorizer
Good penetration
Suited for heat
sensitive articles
AHANA
93. BOILING WATERā¦ā¦ā¦ā¦ā¦..
100ĖC at normal atmospheric
pressure
10 min exposure kills almost all
bacteria & some viruses
Not suggested for tissue
penetrating instruments.
AHANA
95. Glass bead sterilizerā¦..
Rapid , chair side method of sterilization
Usually uses table salt which consists of ~ 1 %
sodium silico aluminate & sodium carbonate
Salt can be replaced by glass beads if < 1 mm in
diameter because , larger beads are not efficient
in transferring heat to endodontic instruments
5- 15 sec at 260Ā° C
AHANA
98. X rays, Gamma rays, High
speed electrons
Effective- heat labile
items; needles, syringes,
culture tray, cannulaā¦
High energy gamma rays
from Cobalt 60
AHANA
99. UV LIGHT
ā¢ Absorbed by proteins & nucleic
acids & kill microbes
ā¢ Purification of air
INFRA RED
ā¢ To sterilize large number of
syringes sealed in metal container.
AHANA
ā¢ Has no penetrating ability
100. AUTOCLAVE TAPE THERMOCOUPLE
CHEMICAL
AHANA
MICROBIAL TESTS
INDICATORS
101. CRITICAL SEMI- CRITICAL NON CRITICAL
Curettes, burs, Amalgam Light switches,
files, scalpels condensers, counter tops,
mouth mirror, drawer pulls or
saliva ejectors cabinets
ā¢Touch sterile ā¢ Touch mucous ā¢Do not come in
areas of body / membrane contact with oral
penetrate tissues without mucosa
& enter vascular penetrating ā¢Touched hands
system tissues. contaminated
ā¢Sterilized & ā¢Should be with saliva/ blood
stored in
AHANA sterilized /
packages disinfected.
102. INSTRUMENT STERILIZATION
CATEGORY METHOD
CRITICAL HEAT STERILIZED & Stored
in packages
SEMI CRITICAL HEAT STERILIZED / Treated
with high level disinfectants
after cleaning
NON CRITICAL Treated with intermediate to
low disinfectant after
cleaning
AHANA
103. Constant contact with oral fluids result in
formation of biofilm within the handpiece.
This holds potential to infect a healthy patient.
Tween 80 & Ponceau 4R
3% hydrogen peroxide
But cannot replace autoclaving.
AHANA
105. STERILIZATION
OF BURS
Critical item
Autoclaving can be done but may
result in rusting.
ETOX sterilization.
AHANA
106. INFECTION CONTROL
FOR IMPRESSIONS
Thoroughly wash under running
water
2% gluteraldehyde for 10 min
Alginate impressions ā Spray an
iodophor
Store items in separate sealed bags.
AHANA
107. DISINFECTION
LEVEL COMPOUND
LOW ETHANOL & ā¢ Antibacterial activity
ISOPROPYL ā¢ Denature proteins
ALCOHOLS ā¢ Maximum 10 min
contact
INTERMEDIATE PHENOLS ā¢ In high concentration
protoplasmic poison
ā¢ Precipitate proteins &
destroy cell wall
HIGH FORMALDEHYDE & ā¢ Toxic & irritant
GLUTERALDEHYDE ā¢ Active against most
vegetative forms &
spores
ā¢ 2 % soln requires
20min for disinfection &
AHANA
6 to 10 hrs for
sterilization.
109. 1. Employers must provide HB immunization to employees
without charge within 10 days of employment.
2. Universal precautions should be observed to prevent contact
with blood & other potentially infectious materials.
3. Employers must implement control systems to reduce
production of contaminated spatter, aerosols etc.
4. Safe handling of needles & other sharps.
5. Disposal of single use needles, wires , sharps etc. in hard
walled, leak proof containers from which needles cant be
easily spilled.
6. Proper hand washing
7. Providing proper personal protective barriers to employees.
8. Proper sterilization & disinfection system
9. Regular laundry services should be arranged.
AHANA
110. BIO
HAZARDOUS
WASTE
BIOMEDICAL
WASTE
MEDICAL
SOLID
WASTE
AHANA
111. BIO HAZARDOUS
WASTE
Laboratory waste
Fluid blood elements
Sharps
Human body parts, tissues etc.
AHANA