Normally, immune responses eradicate infectious pathogens without serious injury to host tissues.
However, these responses are sometimes
inadequately controlled
inappropriately targeted to host tissues
triggered by commensal microorganisms or environmental antigens that are usually harmless.
In these situations, the normally beneficial immune response is the cause of disease.
Disorders caused by immune responses are called hypersensitivity diseases.
This term , hypersensitivity , arose from the clinical definition of immunity as sensitivity, which is based on the observation that an individual who has been exposed to an antigen exhibits a detectable reaction, or is sensitive, to subsequent encounters with that antigen.
Today, we will describe the pathogenesis of different types of hypersensitivity reactions, with an emphasis on the effector mechanisms that cause tissue injury.
A variety of human diseases are caused by immune responses to non-microbial environmental antigens, and involve the type 2 cytokines interleukin-4 (IL-4), IL-5, and IL-13 produced by Th2 cells and innate lymphoid cells (ILCs), immunoglobulin E (IgE), mast cells, and eosinophils.
The antigens that elicit immediate hypersensitivity are called allergens. Most of them are common environmental proteins, animal products, and chemicals that can modify self proteins.
In the effector phase of these responses, mast cells and eosinophils are activated to rapidly release mediators that cause
increased vascular permeability
Vasodilation
bronchial and visceral smooth muscle contraction
This vascular reaction is called immediate hypersensitivity because it begins rapidly, within minutes of antigen challenge in a previously sensitized individual (immediate), and has major pathologic consequences (hypersensitivity).
Following the immediate response, there is a more slowly developing inflammatory component called the late-phase reaction characterized by the accumulation of neutrophils, eosinophils, and macrophages.
Hypersensitivity and autoimmune diseases result from inappropriate immune responses directed against antigens. There are four main types of hypersensitivity reactions classified by the mechanism of tissue injury: immediate (type I) via IgE antibody and mast cells; antibody-mediated (type II) via antibodies binding antigens on cells; immune complex-mediated (type III) via antigen-antibody complexes depositing in tissues; and T cell-mediated (type IV) via T cell-derived cytokines or cytotoxicity. Autoimmune diseases occur when the immune system responds to self-antigens, leading to tissue damage. Examples include systemic lupus erythematosus and rheumatoid arthritis. Transplant rejection also occurs via an immune response against donor antigens
This document discusses autoimmune diseases from several perspectives. It begins by noting that autoimmune diseases affect nearly 3% of the population and can impact nearly any organ. Genetic, environmental, and stochastic factors contribute to autoimmune disease development. Important distinctions are made between autoimmunity, which may be asymptomatic, and autoimmune disease, which causes tissue damage and inflammation. The document also discusses mechanisms of tissue injury, differences between systemic and organ-specific diseases, and various treatment approaches including anti-inflammatory drugs, immunosuppressants, biologics, hematopoietic stem cell transplantation, and intravenous immunoglobulin.
This document discusses autoimmune diseases from several perspectives. It notes that nearly 100 forms of autoimmune disease exist, affecting up to 3% of the population. Women are disproportionately affected, making up 75% of cases. Both genetic and environmental factors contribute to disease development. Autoimmunity, in which the immune system responds to self-antigens, is common and usually asymptomatic, whereas autoimmune disease causes inflammation and tissue damage. Treatment involves nonsteroidal anti-inflammatory drugs, corticosteroids, immunosuppressants, biologic agents, and autologous stem cell transplantation in severe cases.
The document summarizes key aspects of the immune system, including innate immunity as the first line of defense against microbes, and adaptive immunity which develops after exposure. It describes the two types of adaptive immunity - humoral mediated by B cells and antibodies, and cellular mediated by T cells. Hypersensitivity reactions are classified into four types based on their immune mechanisms. Systemic lupus erythematosus is discussed as an autoimmune disease characterized by autoantibodies and varying clinical manifestations that can affect multiple organ systems. Chronic discoid lupus erythematosus and subacute cutaneous lupus erythematosus represent syndromes where skin involvement is prominent.
This document discusses hypersensitivity and immune-mediated disorders. It defines hypersensitivity as an immunologic reaction that causes tissue damage upon reexposure to an antigen. There are four types of hypersensitivity reactions classified by Gell and Coombs: type I involves IgE antibodies, type II involves IgG antibodies and complement, type III involves immune complex formation, and type IV involves delayed-type hypersensitivity and T cells. Causes of hypersensitivity diseases include reactions against self-antigens due to failures in self-tolerance (autoimmunity), reactions against microbes, and reactions against environmental antigens. Diseases can be caused by antibodies against cells/tissues or immune complexes deposited in tissues.
This document discusses the four types of hypersensitivity reactions:
1. Type I reactions are immediate and mediated by IgE antibodies. They include allergic reactions and anaphylaxis.
2. Type II reactions are cytotoxic and mediated by IgG and IgM antibodies attacking cell surfaces, causing cell lysis. Examples include autoimmune hemolytic anemia.
3. Type III reactions involve antigen-antibody complex deposition, complement activation, and inflammatory cell injury. Examples are immune complex glomerulonephritis.
4. Type IV reactions are delayed, cell-mediated responses without antibodies. Examples include tuberculin reactions and transplant rejection.
The document discusses nursing care for children with immune disorders. It describes the immune system and how it protects the body from infection. Immune disorders can be primary (congenital) such as deficiencies in B or T lymphocytes, or secondary (acquired) from infections, medications, or other causes. Nursing focuses on preventing infection through hygiene and monitoring for symptoms. Education of families is also important so they can care for the child's special needs.
Hypersensitivity and autoimmune diseases result from inappropriate immune responses directed against antigens. There are four main types of hypersensitivity reactions classified by the mechanism of tissue injury: immediate (type I) via IgE antibody and mast cells; antibody-mediated (type II) via antibodies binding antigens on cells; immune complex-mediated (type III) via antigen-antibody complexes depositing in tissues; and T cell-mediated (type IV) via T cell-derived cytokines or cytotoxicity. Autoimmune diseases occur when the immune system responds to self-antigens, leading to tissue damage. Examples include systemic lupus erythematosus and rheumatoid arthritis. Transplant rejection also occurs via an immune response against donor antigens
This document discusses autoimmune diseases from several perspectives. It begins by noting that autoimmune diseases affect nearly 3% of the population and can impact nearly any organ. Genetic, environmental, and stochastic factors contribute to autoimmune disease development. Important distinctions are made between autoimmunity, which may be asymptomatic, and autoimmune disease, which causes tissue damage and inflammation. The document also discusses mechanisms of tissue injury, differences between systemic and organ-specific diseases, and various treatment approaches including anti-inflammatory drugs, immunosuppressants, biologics, hematopoietic stem cell transplantation, and intravenous immunoglobulin.
This document discusses autoimmune diseases from several perspectives. It notes that nearly 100 forms of autoimmune disease exist, affecting up to 3% of the population. Women are disproportionately affected, making up 75% of cases. Both genetic and environmental factors contribute to disease development. Autoimmunity, in which the immune system responds to self-antigens, is common and usually asymptomatic, whereas autoimmune disease causes inflammation and tissue damage. Treatment involves nonsteroidal anti-inflammatory drugs, corticosteroids, immunosuppressants, biologic agents, and autologous stem cell transplantation in severe cases.
The document summarizes key aspects of the immune system, including innate immunity as the first line of defense against microbes, and adaptive immunity which develops after exposure. It describes the two types of adaptive immunity - humoral mediated by B cells and antibodies, and cellular mediated by T cells. Hypersensitivity reactions are classified into four types based on their immune mechanisms. Systemic lupus erythematosus is discussed as an autoimmune disease characterized by autoantibodies and varying clinical manifestations that can affect multiple organ systems. Chronic discoid lupus erythematosus and subacute cutaneous lupus erythematosus represent syndromes where skin involvement is prominent.
This document discusses hypersensitivity and immune-mediated disorders. It defines hypersensitivity as an immunologic reaction that causes tissue damage upon reexposure to an antigen. There are four types of hypersensitivity reactions classified by Gell and Coombs: type I involves IgE antibodies, type II involves IgG antibodies and complement, type III involves immune complex formation, and type IV involves delayed-type hypersensitivity and T cells. Causes of hypersensitivity diseases include reactions against self-antigens due to failures in self-tolerance (autoimmunity), reactions against microbes, and reactions against environmental antigens. Diseases can be caused by antibodies against cells/tissues or immune complexes deposited in tissues.
This document discusses the four types of hypersensitivity reactions:
1. Type I reactions are immediate and mediated by IgE antibodies. They include allergic reactions and anaphylaxis.
2. Type II reactions are cytotoxic and mediated by IgG and IgM antibodies attacking cell surfaces, causing cell lysis. Examples include autoimmune hemolytic anemia.
3. Type III reactions involve antigen-antibody complex deposition, complement activation, and inflammatory cell injury. Examples are immune complex glomerulonephritis.
4. Type IV reactions are delayed, cell-mediated responses without antibodies. Examples include tuberculin reactions and transplant rejection.
The document discusses nursing care for children with immune disorders. It describes the immune system and how it protects the body from infection. Immune disorders can be primary (congenital) such as deficiencies in B or T lymphocytes, or secondary (acquired) from infections, medications, or other causes. Nursing focuses on preventing infection through hygiene and monitoring for symptoms. Education of families is also important so they can care for the child's special needs.
Basic Principles of Hypersensitivity ReactionsHadi Munib
Hypersensitivity reactions occur when the immune system mounts an excessive or inappropriate response against antigens. There are four main types of hypersensitivity reactions:
Type I reactions are immediate and antigen-specific, mediated by IgE antibodies and mast cells. Type II reactions are caused by antibodies against antigens on cells, targeting them for destruction. Type III reactions involve immune complex deposition in tissues, activating complement and causing inflammation. Type IV reactions are T-cell mediated, characterized by cytokine-induced inflammation or direct cytotoxicity by CD8+ T cells. Each type can result in different clinical manifestations and tissue damage.
This document provides an overview of autoimmune diseases. It defines autoimmune diseases as conditions where the immune system mistakenly attacks and destroys healthy body tissue. The causes include genetic factors, environmental triggers like infections, and defects in immunologic tolerance. Some specific autoimmune diseases discussed are rheumatoid arthritis, type 1 diabetes, Hashimoto's thyroiditis, Graves' disease, myasthenia gravis, and systemic sclerosis. The mechanisms, clinical features, pathology, and treatment options are described for each condition.
This document discusses the different types of hypersensitivity reactions:
1. Type I reactions are immediate and antibody-mediated, involving IgE. They cause conditions like allergic asthma and anaphylaxis.
2. Type II reactions are cytotoxic and involve IgG/IgM binding to cell surfaces and activating complement. They can lyse cells.
3. Type III reactions involve soluble immune complexes activating complement and causing inflammation. They include serum sickness.
4. Type IV reactions are delayed, cell-mediated responses involving sensitized T cells and cytokines. They cause conditions like contact dermatitis.
Immunologically mediated tissue injury, or hypersensitivity, is the basis for many immune-mediated diseases. There are four main types of hypersensitivity reactions: immediate (type I), antibody-mediated (type II), immune complex-mediated (type III), and cell-mediated (type IV). Immediate hypersensitivity is initiated by IgE antibodies and mast cells, antibody-mediated disorders involve cell damage by antibodies, immune complex disease involves antigen-antibody complex deposition, and cell-mediated disorders involve tissue damage by T cells. Autoimmune diseases result from a break in immune tolerance and involve organ-specific or systemic pathology. Systemic lupus erythematosus is a classical systemic autoimmune disease characterized by various
HYPERSENSITIVITY 204. and the immune systempetshelter54
This document summarizes hypersensitivity reactions, which are harmful immune reactions caused by an excessive or improper response to antigens. There are four main types of hypersensitivity reactions:
1. Type I reactions are antibody-mediated and involve IgE, mast cells, and basophils. They cause immediate allergic reactions.
2. Type II reactions are also antibody-mediated and involve IgG/IgM binding to cell surfaces, leading to cell destruction.
3. Type III reactions involve immune complex deposition in tissues, activating the complement system and causing inflammation.
4. Type IV reactions are cell-mediated and involve T cells/cytokines, causing delayed hypersensitivity reactions like contact dermatitis.
1. Type I Hypersensitivity:
Type I hypersensitive reactions are the commonest type among all types which is mainly induced by certain type of antigens i.e. allergens. Actually anaphylaxis means “opposite of protection” and is mediated by IgE antibodies through interaction with an allergen
This document discusses hypersensitivity reactions and autoimmune diseases. It describes the different types of hypersensitivity reactions (Type I-IV) and their mechanisms and examples. It also discusses autoimmune diseases, noting they arise due to genetic susceptibility and environmental triggers damaging self tolerance. Autoimmune diseases can affect many organ systems. The document also briefly touches on tissue transplant rejection, immunodeficiencies, HIV/AIDS, and amyloidosis.
Autoimmunity and Autoimmune diseases.pptxrajmonu7858
A concise ppt about autoimmunity. It incudes information about tolerance, etiology behind autoimmune diseases, types of autoimmune diseases and few examples of diseases.
This document discusses hypersensitivity reactions and autoimmune diseases. It describes the four types of hypersensitivity reactions according to the Gell and Coombs classification: Type I (immediate), Type II (cytotoxic), Type III (immune complex-mediated), and Type IV (delayed type hypersensitivity). It provides details on the mechanisms and examples of each type. The document then discusses immunological tolerance, including central and peripheral tolerance. It explains how a breakdown in tolerance can lead to autoimmune diseases and provides examples like Graves' disease, myasthenia gravis, hemolytic anemia, and systemic lupus erythematosus.
This document discusses tolerance and autoimmune diseases. It explains that tolerance is a lack of immune response to antigens, including self-antigens, which prevents autoimmunity. Central and peripheral tolerance mechanisms aim to eliminate or inactivate self-reactive immune cells. When tolerance breaks down, autoantibodies and self-reactive T-cells can damage tissues, as seen in autoimmune diseases like rheumatoid arthritis, SLE, and multiple sclerosis. Genetics, infections, and environmental factors may influence autoimmunity. The immune system attacks via antibody-mediated or cell-mediated responses. Diagnosis involves testing for autoantibodies and immune complexes.
Introduction to hypersensitive reactionsDeepika Rana
This document provides an overview of hypersensitive reactions and their classification. It discusses the five main types of hypersensitivity reactions: type I-IV reactions which are antibody-mediated and immediate (types I-III) or delayed (type IV); and type V which involves antibody stimulation of cell surface receptors. Type I is allergy mediated by IgE antibodies. Type II involves IgG/IgM binding to cell surfaces. Type III occurs via immune complex deposition. Type IV is cell-mediated via T cells. Examples of each type are given.
Classic problems and emerging areas of immune system by Kainat RamzanKainatRamzan3
The immune system can be simplistically viewed as having two “lines of defense” innate immunity and adaptive immunity. The immune system refers to a collection of cells and proteins that function to protect the skin, respiratory passages, intestinal tract, and other areas from foreign antigens, such as microbes, viruses, cancer cells, and toxins.
Hypersensitivity reactions are common and can affect 15% of the world's population. They are classified into four main types: Type I or immediate hypersensitivity; Type II or antibody-mediated cytotoxic reactions; Type III or immune complex-mediated reactions; and Type IV or delayed hypersensitivity reactions. Type I reactions are IgE-mediated and involve mast cell degranulation, while Type IV reactions are T cell-mediated and involve inflammatory responses. Common oral hypersensitivity reactions include lichen planus, erythema multiforme, recurrent aphthous stomatitis, and geographic tongue. Treatment involves identifying and avoiding triggers when possible, along with topical corticosteroids for symptom management.
Diseases of the Immune System: Hypersensitivity ReactionsDr. Roopam Jain
This document discusses hypersensitivity and autoimmune diseases. It defines hypersensitivity as injurious immune reactions caused by exogenous or endogenous antigens. There are four types of hypersensitivity reactions: type I involves IgE antibodies and mast cells; type II involves IgG/IgM antibodies damaging cells; type III involves IgG/IgM antibody-antigen complexes depositing in tissues; type IV involves sensitized T cells damaging tissues. Autoimmune diseases result from a loss of immune tolerance and involve genetic and environmental factors. Examples of organ-specific diseases include Hashimoto's thyroiditis and Graves' disease, while systemic lupus erythematosus is provided as an example of a systemic autoimmune disease.
This document discusses the different types of hypersensitivity reactions as classified by Gell and Coombs. It provides details on type I (immediate), type II (antibody-mediated), type III (immune complex-mediated), and type IV (delayed) hypersensitivity reactions, including the antibodies or cells involved, examples of diseases, and a brief description of the immunological reaction for each type. It also discusses contact stomatitis as an uncommon allergic reaction affecting the inside of the mouth.
Hypersensitivity refers to excessive or inappropriate immune responses that are harmful to the body. There are four main types of hypersensitivity reactions based on their pathogenic mechanisms:
1. Type I reactions are immediate or anaphylactic and mediated by IgE antibodies, causing release of inflammatory mediators from mast cells.
2. Type II reactions are antibody-dependent cytotoxic reactions, where antibodies bind to antigens on self cells leading to complement activation and cell lysis.
3. Type III reactions are immune complex-mediated, where circulating antigen-antibody complexes deposit in tissues and activate complement, causing inflammation.
4. Type IV reactions are cell-mediated and involve activation of sensitized T cells by antigens,
Type IV hypersensitivity reactions are T cell-mediated responses that cause tissue damage. They occur when T cells respond to antigens in tissues, releasing cytokines that activate macrophages and cause inflammation. This results in conditions like contact dermatitis and delayed-type hypersensitivity reactions. The tissue injury in these diseases is caused by macrophages and neutrophils that are activated by T cells. Treatment focuses on reducing inflammation and inhibiting T cell responses.
Drug hypersensitivity is an immune-mediated reaction to a drug that ranges from mild to severe symptoms like rash and anaphylaxis. It differs from expected adverse drug effects or interactions. Diagnosis is clinical with occasional skin testing use, and treatment involves drug discontinuation, supportive care like antihistamines, and sometimes desensitization.
Drug hypersensitivity is an immune-mediated reaction to a drug that ranges from mild to severe symptoms like rash and anaphylaxis. It differs from expected adverse drug effects or interactions. Diagnosis is clinical with occasional skin testing use, and treatment involves drug discontinuation, supportive care like antihistamines, and sometimes desensitization.
Basic Principles of Hypersensitivity ReactionsHadi Munib
Hypersensitivity reactions occur when the immune system mounts an excessive or inappropriate response against antigens. There are four main types of hypersensitivity reactions:
Type I reactions are immediate and antigen-specific, mediated by IgE antibodies and mast cells. Type II reactions are caused by antibodies against antigens on cells, targeting them for destruction. Type III reactions involve immune complex deposition in tissues, activating complement and causing inflammation. Type IV reactions are T-cell mediated, characterized by cytokine-induced inflammation or direct cytotoxicity by CD8+ T cells. Each type can result in different clinical manifestations and tissue damage.
This document provides an overview of autoimmune diseases. It defines autoimmune diseases as conditions where the immune system mistakenly attacks and destroys healthy body tissue. The causes include genetic factors, environmental triggers like infections, and defects in immunologic tolerance. Some specific autoimmune diseases discussed are rheumatoid arthritis, type 1 diabetes, Hashimoto's thyroiditis, Graves' disease, myasthenia gravis, and systemic sclerosis. The mechanisms, clinical features, pathology, and treatment options are described for each condition.
This document discusses the different types of hypersensitivity reactions:
1. Type I reactions are immediate and antibody-mediated, involving IgE. They cause conditions like allergic asthma and anaphylaxis.
2. Type II reactions are cytotoxic and involve IgG/IgM binding to cell surfaces and activating complement. They can lyse cells.
3. Type III reactions involve soluble immune complexes activating complement and causing inflammation. They include serum sickness.
4. Type IV reactions are delayed, cell-mediated responses involving sensitized T cells and cytokines. They cause conditions like contact dermatitis.
Immunologically mediated tissue injury, or hypersensitivity, is the basis for many immune-mediated diseases. There are four main types of hypersensitivity reactions: immediate (type I), antibody-mediated (type II), immune complex-mediated (type III), and cell-mediated (type IV). Immediate hypersensitivity is initiated by IgE antibodies and mast cells, antibody-mediated disorders involve cell damage by antibodies, immune complex disease involves antigen-antibody complex deposition, and cell-mediated disorders involve tissue damage by T cells. Autoimmune diseases result from a break in immune tolerance and involve organ-specific or systemic pathology. Systemic lupus erythematosus is a classical systemic autoimmune disease characterized by various
HYPERSENSITIVITY 204. and the immune systempetshelter54
This document summarizes hypersensitivity reactions, which are harmful immune reactions caused by an excessive or improper response to antigens. There are four main types of hypersensitivity reactions:
1. Type I reactions are antibody-mediated and involve IgE, mast cells, and basophils. They cause immediate allergic reactions.
2. Type II reactions are also antibody-mediated and involve IgG/IgM binding to cell surfaces, leading to cell destruction.
3. Type III reactions involve immune complex deposition in tissues, activating the complement system and causing inflammation.
4. Type IV reactions are cell-mediated and involve T cells/cytokines, causing delayed hypersensitivity reactions like contact dermatitis.
1. Type I Hypersensitivity:
Type I hypersensitive reactions are the commonest type among all types which is mainly induced by certain type of antigens i.e. allergens. Actually anaphylaxis means “opposite of protection” and is mediated by IgE antibodies through interaction with an allergen
This document discusses hypersensitivity reactions and autoimmune diseases. It describes the different types of hypersensitivity reactions (Type I-IV) and their mechanisms and examples. It also discusses autoimmune diseases, noting they arise due to genetic susceptibility and environmental triggers damaging self tolerance. Autoimmune diseases can affect many organ systems. The document also briefly touches on tissue transplant rejection, immunodeficiencies, HIV/AIDS, and amyloidosis.
Autoimmunity and Autoimmune diseases.pptxrajmonu7858
A concise ppt about autoimmunity. It incudes information about tolerance, etiology behind autoimmune diseases, types of autoimmune diseases and few examples of diseases.
This document discusses hypersensitivity reactions and autoimmune diseases. It describes the four types of hypersensitivity reactions according to the Gell and Coombs classification: Type I (immediate), Type II (cytotoxic), Type III (immune complex-mediated), and Type IV (delayed type hypersensitivity). It provides details on the mechanisms and examples of each type. The document then discusses immunological tolerance, including central and peripheral tolerance. It explains how a breakdown in tolerance can lead to autoimmune diseases and provides examples like Graves' disease, myasthenia gravis, hemolytic anemia, and systemic lupus erythematosus.
This document discusses tolerance and autoimmune diseases. It explains that tolerance is a lack of immune response to antigens, including self-antigens, which prevents autoimmunity. Central and peripheral tolerance mechanisms aim to eliminate or inactivate self-reactive immune cells. When tolerance breaks down, autoantibodies and self-reactive T-cells can damage tissues, as seen in autoimmune diseases like rheumatoid arthritis, SLE, and multiple sclerosis. Genetics, infections, and environmental factors may influence autoimmunity. The immune system attacks via antibody-mediated or cell-mediated responses. Diagnosis involves testing for autoantibodies and immune complexes.
Introduction to hypersensitive reactionsDeepika Rana
This document provides an overview of hypersensitive reactions and their classification. It discusses the five main types of hypersensitivity reactions: type I-IV reactions which are antibody-mediated and immediate (types I-III) or delayed (type IV); and type V which involves antibody stimulation of cell surface receptors. Type I is allergy mediated by IgE antibodies. Type II involves IgG/IgM binding to cell surfaces. Type III occurs via immune complex deposition. Type IV is cell-mediated via T cells. Examples of each type are given.
Classic problems and emerging areas of immune system by Kainat RamzanKainatRamzan3
The immune system can be simplistically viewed as having two “lines of defense” innate immunity and adaptive immunity. The immune system refers to a collection of cells and proteins that function to protect the skin, respiratory passages, intestinal tract, and other areas from foreign antigens, such as microbes, viruses, cancer cells, and toxins.
Hypersensitivity reactions are common and can affect 15% of the world's population. They are classified into four main types: Type I or immediate hypersensitivity; Type II or antibody-mediated cytotoxic reactions; Type III or immune complex-mediated reactions; and Type IV or delayed hypersensitivity reactions. Type I reactions are IgE-mediated and involve mast cell degranulation, while Type IV reactions are T cell-mediated and involve inflammatory responses. Common oral hypersensitivity reactions include lichen planus, erythema multiforme, recurrent aphthous stomatitis, and geographic tongue. Treatment involves identifying and avoiding triggers when possible, along with topical corticosteroids for symptom management.
Diseases of the Immune System: Hypersensitivity ReactionsDr. Roopam Jain
This document discusses hypersensitivity and autoimmune diseases. It defines hypersensitivity as injurious immune reactions caused by exogenous or endogenous antigens. There are four types of hypersensitivity reactions: type I involves IgE antibodies and mast cells; type II involves IgG/IgM antibodies damaging cells; type III involves IgG/IgM antibody-antigen complexes depositing in tissues; type IV involves sensitized T cells damaging tissues. Autoimmune diseases result from a loss of immune tolerance and involve genetic and environmental factors. Examples of organ-specific diseases include Hashimoto's thyroiditis and Graves' disease, while systemic lupus erythematosus is provided as an example of a systemic autoimmune disease.
This document discusses the different types of hypersensitivity reactions as classified by Gell and Coombs. It provides details on type I (immediate), type II (antibody-mediated), type III (immune complex-mediated), and type IV (delayed) hypersensitivity reactions, including the antibodies or cells involved, examples of diseases, and a brief description of the immunological reaction for each type. It also discusses contact stomatitis as an uncommon allergic reaction affecting the inside of the mouth.
Hypersensitivity refers to excessive or inappropriate immune responses that are harmful to the body. There are four main types of hypersensitivity reactions based on their pathogenic mechanisms:
1. Type I reactions are immediate or anaphylactic and mediated by IgE antibodies, causing release of inflammatory mediators from mast cells.
2. Type II reactions are antibody-dependent cytotoxic reactions, where antibodies bind to antigens on self cells leading to complement activation and cell lysis.
3. Type III reactions are immune complex-mediated, where circulating antigen-antibody complexes deposit in tissues and activate complement, causing inflammation.
4. Type IV reactions are cell-mediated and involve activation of sensitized T cells by antigens,
Type IV hypersensitivity reactions are T cell-mediated responses that cause tissue damage. They occur when T cells respond to antigens in tissues, releasing cytokines that activate macrophages and cause inflammation. This results in conditions like contact dermatitis and delayed-type hypersensitivity reactions. The tissue injury in these diseases is caused by macrophages and neutrophils that are activated by T cells. Treatment focuses on reducing inflammation and inhibiting T cell responses.
Drug hypersensitivity is an immune-mediated reaction to a drug that ranges from mild to severe symptoms like rash and anaphylaxis. It differs from expected adverse drug effects or interactions. Diagnosis is clinical with occasional skin testing use, and treatment involves drug discontinuation, supportive care like antihistamines, and sometimes desensitization.
Drug hypersensitivity is an immune-mediated reaction to a drug that ranges from mild to severe symptoms like rash and anaphylaxis. It differs from expected adverse drug effects or interactions. Diagnosis is clinical with occasional skin testing use, and treatment involves drug discontinuation, supportive care like antihistamines, and sometimes desensitization.
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TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kol...rightmanforbloodline
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
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2. References
• Cellular and Molecular Immunology, 10th Edition-
Chapter 19 & 20: Hypersensitivity Disorders, p: 437-
457 & 459-479
• Harrison's Principles of Internal Medicine, 19th
Edition-Part 15: Immune-mediated, Inflammatory,
and Rheumatologic Disorders; Section 2 :Disorders
of Immune-Mediated Injury, p: 2113
• Clinical Immunology: Principles and Practice, 5th
Edition- Chapter 41: Immunological Mechanisms of
Airway Diseases and Pathways to Therapy; p: 571
• Kelley and Firestein's Textbook of Rheumatology, th
Edition- Chapter 19; Immunologic mechanisms of
tissue inflammation & dysfunction, p: 312,
3. • Normally, immune responses eradicate infectious pathogens without serious injury to host tissues.
• However, these responses are sometimes
• inadequately controlled
• inappropriately targeted to host tissues
• triggered by commensal microorganisms or environmental antigens that are usually harmless.
In these situations, the normally beneficial immune response is the cause of disease.
• Disorders caused by immune responses are called hypersensitivity diseases.
• This term , hypersensitivity , arose from the clinical definition of immunity as sensitivity, which is based
on the observation that an individual who has been exposed to an antigen exhibits a detectable reaction,
or is sensitive, to subsequent encounters with that antigen.
• Today, we will describe the pathogenesis of different types of hypersensitivity reactions, with an
emphasis on the effector mechanisms that cause tissue injury.
Just to recap …
4. Causes of Hypersensitivity Diseases
• Immune responses that are the cause of hypersensitivity diseases may be specific for antigens from different
sources.
• Reactions against self antigens, autoimmunity:
• Failure of the normal mechanisms of self-tolerance results in T cell and B cell reactions against
one’s own cells and tissues that are called autoimmunity , and the diseases caused by these
reactions are referred to as autoimmune diseases.
• Autoimmune diseases are estimated to affect at least 5% of the population in higher-income
countries, and the incidence of these disorders is rising.
• Many of these diseases are more common in women than in men; the mechanisms underlying this
gender bias remain obscure.
• Autoimmune diseases are usually chronic and often debilitating and are an enormous medical and
economic burden.
• Although these disorders have been difficult to treat in the past, many new effective therapies
have been developed since the 1990s on scientific advances in immunology. Here, we will refer to
various autoimmune disorders to illustrate how immune reactions against self cause disease.
5. Causes of Hypersensitivity Diseases
• Reactions against microbes.
• Immune responses against microbial antigens may cause disease if the reactions are excessive or the
microbes are unusually persistent.
• T cell responses against persistent microbes may give rise to severe inflammation, sometimes with the
formation of granulomas; this is the cause of tissue injury in tuberculosis and some other chronic
infections.
• If antibodies are produced against microbial antigens, the antibodies may bind to the antigens to produce
immune complexes, which deposit in tissues and trigger inflammation.
• Rarely, antibodies or T cells against a microbe will cross-react with a host tissue.
− In some cases like inflammatory bowel disease, the immune response is directed against commensal
bacteria that normally reside in the gut and cause no harm.
− Sometimes the mechanisms that an immune response uses to eradicate a pathogenic microbe require
killing infected cells, and therefore such responses inevitably injure host tissues. For example, in viral
hepatitis, the virus that infects liver cells is not cytopathic, but it is recognized as foreign by the immune
system. Cytotoxic T lymphocytes (CTLs) eliminate infected cells, and this normal immune response
damages the liver.
6. Causes of Hypersensitivity Diseases
• Reactions against non-microbial environmental antigens
• Most healthy individuals do not react against common, generally harmless environmental substances,
but almost 20% of the population is abnormally responsive to one or more of these substances. These
individuals produce IgE antibodies that cause allergic diseases.
• Some individuals become sensitized to environmental antigens and chemicals that contact the skin and
develop T cell reactions that lead to cytokine-mediated inflammation, resulting in contact sensitivity.
• Idiosyncratic immunologic reactions against therapeutic drugs are also a frequent clinical problem.
In all of these conditions, the mechanisms of tissue injury are the same as those that normally function to
eliminate infectious pathogens. These mechanisms include innate and adaptive immune responses
involving phagocytes, antibodies, T lymphocytes, mast cells, and various other effector cells, and mediators
of inflammation..
7. Causes of Hypersensitivity Diseases
• Autoimmunity ( reactions against self antigens)
• Reactions against microbes
• Reactions against non-microbial environmental antigens
• The problem in hypersensitivity diseases is that the immune response is not controlled appropriately or
is targeted to normal tissues.
• Because the stimuli for these abnormal immune responses are often impossible to eliminate
• self antigens
• commensal microbes
• environmental antigens
and the immune system has many built-in positive feedback loops (amplification mechanisms), once a
pathologic immune response starts, it is difficult to control or to terminate it.
• Therefore, these hypersensitivity diseases tend to be chronic and progressive and pose major
therapeutic challenges in clinical medicine.
8. Mechanisms & Classification of Hypersensitivity reactions
• Hypersensitivity diseases are commonly classified according to
• the type of immune response
• and the effector mechanism responsible for cell and tissue injury .
The various hypersensitivity states were originally classified into types I–IV (Figure) by Gell and Coombs and
this classification remains broadly useful.
9. • Immediate (type I) hypersensitivity is caused by IgE antibodies specific for nonmicobial environmental antigens and is the most
prevalent type of hypersensitivity disease. Immediate hypersensitivity diseases, are often caused by activation of interleukin-4 (IL-4), IL-5,
and IL-13 producing Th2 cells and the production of IgE antibodies, which activate mast cells and eosinophils and induce inflammation.
• Antibody-mediated (type II) hypersensitivity. IgG and IgM antibodies specific for cell surface or extracellular matrix antigens can cause
tissue injury by activating the complement system, targeting cells for phagocytosis by leukocytes, recruiting inflammatory
cells, and interfering with normal cellular functions.
• Immune complex–mediated (type III) hypersensitivity. IgM and IgG antibodies specific for soluble antigens in the blood form
complexes with the antigens, and the immune complexes may deposit in blood vessel walls in various tissues, causing inflammation,
thrombosis, and tissue injury.
• T cell–mediated (type IV) hypersensitivity. In these disorders, tissue injury may be due to CD4+ T lymphocytes, which secrete cytokines
that induce inflammation, or CD8+ CTLs, which kill target cells.
Mechanisms & Classification of Hypersensitivity reactions
10. Mechanisms & Classification of Hypersensitivity reactions
• Type I: IgE‐mediated mast cell
degranulation.
• Type II: antibody‐dependent cytotoxicity
which can be mediated by killer cells
carrying out ADCC, by opsonization for
phagocytosis, or by activation of the
classical pathway of complement with the
generation of the membrane attack
complex.
• Type III: immune complex mediated which
can result in activation of phagocytic cells
leading to an inflammatory response (as
well as platelet aggregation and mast cell
activation, not shown).
• Type IV: delayed type (cell‐mediated) involving
the release of cytokines from T‐cells.
• Type V: stimulatory hypersensitivity in which
antibodies act as agonists for cell surface
receptors.
• Innate hypersensitivity resulting from, for
example, excessive activation of pattern
recognition receptors (PRR).
11. These mechanisms have been broadly grouped into hypersensitivity types II to IV, which encompass antibody-, immune complex–, and T cell–
mediated processes, respectively (Table 19-2, keley ).
13. The word “allergy” first appeared on July 24, 1906 in the Münchener Medizinische Wochenschrift in an essay
written by Clemens von Pirquet, a pediatrician from Vienna. (Reproduced with permission from Ring J: Allergy in
Practice. Springer Berlin, Heidelberg, New York, 2005.)
14. Allergies?
• A variety of human diseases are caused by immune responses to
non-microbial environmental antigens, and involve the type 2
cytokines interleukin-4 (IL-4), IL-5, and IL-13 produced by Th2 cells
and innate lymphoid cells (ILCs), immunoglobulin E (IgE), mast cells,
and eosinophils.
• The antigens that elicit immediate hypersensitivity are called
allergens. Most of them are common environmental proteins,
animal products, and chemicals that can modify self proteins.
• In the effector phase of these responses, mast cells and eosinophils
are activated to rapidly release mediators that cause
• increased vascular permeability
• Vasodilation
• bronchial and visceral smooth muscle contraction
• This vascular reaction is called immediate hypersensitivity because
it begins rapidly, within minutes of antigen challenge in a previously
sensitized individual (immediate), and has major pathologic
consequences (hypersensitivity).
• Following the immediate response, there is a more slowly
developing inflammatory component called the late-phase reaction
characterized by the accumulation of neutrophils, eosinophils, and
macrophages.
15. Allergies?
• In clinical medicine, these reactions ( both immediate and late-
phase reactions) are called allergy or atopy,
• The associated diseases are called allergic, atopic, or immediate
hypersensitivity diseases. (The term allergy is often imprecisely
used in clinical practice to describe other hypersensitivity
reactions to environmental antigens, such as contact
hypersensitivity.)
• Repeated bouts of IgE- and mast cell– dependent reactions can
lead to chronic allergic diseases, with tissue damage and
remodeling. The most common of these chronic disorders are
• eczema (also known as atopic dermatitis),
• hay fever (allergic rhinitis)
• allergic asthma
16. How Many People Are Affected By Allergies?
• Although atopy originally
meant unusual, we now
realize that allergy is the
most common disorder of
immunity, affecting at least
20% of all individuals in the
United States and Europe,
and its prevalence is
increasing worldwide.
• 5% of the worldwide
population have allergies
related to insect stings. In the
US, around 40 deaths occur
annually due to a severe
allergic reaction (anaphylaxis)
to insect stings.
https://allergyasthmanetwork.org/allergies/allergy-statistics//
17. • How Many People Are Affected By Allergies?
• 1 out of 5 Americans have either allergy or asthma related
symptoms.
• Food allergies affect around 2.5% of the global population.
• Around 50% of anaphylaxis reactions are caused by food
allergies.
• In the US, 30% of people with allergies are allergic to dogs
and cats.
• Globally, 10%–20% of people are allergic to dogs and cats.
• β-Lactam allergy remains the most common reported
medication allergy in the United States, with an estimated
prevalence of 10%.
18. Type I hypersensitivity
• Allergic reaction should be categorized in four
steps:
1. IgE production in response to innocuous
antigen
2. IgE binding to mast cells receptors (also
eosinophils, basophils)
3. Repeat exposure to same allergen
4. Activation of mast cell, release of mediators :
1. Immediate Phase
2. Late Phase
Local: hay fever, bronchial asthma
Systemic: circulatory shock
20. IgE
• In 1921, Kustner, who was allergic to fish, injected his own serum into the skin of
Prausnitz, who was allergic to grass pollen but not fish, and demonstrated that it
was possible to transfer immediate hypersensitivity passively (the Prausnitz–
Kustner or P–K test). Prausnitz also noticed that an immediate wheal and flare
occurred at the site of passive sensitization when he ate fish. This showed that
some protein or part of fish proteins sufficient to trigger mast cells can be
absorbed into the circulation.
• Over the next 30 years, it was established that P–K activity was a general property
of the serum of patients with immediate hypersensitivity and that it was allergen
specific .
• Serum, suspected to contain IgE antibodies, is drawn from the allergic (atopic)
patient. This serum is injected intradermally into a non-allergic person. The
suspected antigens are then intradermally injected into the non-allergic person
24–48 hours later. If the person being tested for an allergy has made antibodies
for the antigen, this will cause a local reaction in the non-allergic person when the
antibodies mix with the antigen. This demonstrates a type I hypersensitivity
reaction, and the allergic reaction to the injected antigen is confirmed for the
patient being tested. A positive PK test usually appears as a wheal and flare.
• The test has been replaced by the safer skin prick test.
A type I hypersensitivity reaction produces a
raised wheal 5–7 mm in diameter and with a
well-defined edge after about 15 minutes
21. IgE
• In 1967, Ishizaka and his colleagues purified the P–K
activity from a patient with ragweed hay fever and proved
that this was a novel isotype of immunoglobulin – IgE.
• However, it was obvious that the concentration of IgE in
serum was very low i.e. 1 μg/mL.
• IgE is distinct from the other dimeric immunoglobulins,
because it has:
• an extra constant region domain;
• a different structure to the hinge region;
• And the primary binding sites for both FcεR1 and
FcεR2 are on the C3 domain of IgE. This domain has
an unusual form of internal flexibility, such that on
binding with IgE it rearranges and becomes
thermodynamically more stable.
IgE can be cleaved by enzymes to give the fragments F(ab0)2, Fc,
and Fc0. Note the absence of a hinge region.
22. 1- IgE production
• Atopic individuals produce high levels of IgE in response to
environmental allergens, whereas normal individuals generally
produce other Ig isotypes, such as IgM and IgG, and only small
amounts of IgE.
• IgE is of central importance in atopy because this isotype is
responsible for sensitizing mast cells and it specifically recognizes
antigen for immediate hypersensitivity reactions.
• IgE is the antibody isotype that contains the ε heavy chain . It binds to
specific Fc receptors on mast cells and activates these cells upon
antigen binding.
• The quantity of IgE synthesized depends on the propensity of an
individual to generate allergen-specific Tfh cells that produce IL-4 and
IL-13, because these cytokines stimulate B cell antibody class
switching to IgE .
• The development of IL-4– and IL-13–expressing T cell responses
against particular antigens may be influenced by a variety of factors,
including
• Genetic Susceptibility to Allergic Diseases
• The Environmental Factors in Allergy
• The nature of the antigens.
24. 1- IgE production
1-1- Genetic Susceptibility to Allergic Diseases
• Family studies have shown clear autosomal transmission of atopy, although the full
inheritance pattern is multigenic.
• Within the same family, the target organ of atopic disease is variable. Thus,
• allergic rhinitis (hay fever)
• Asthma
• atopic dermatitis (eczema)
All these individuals, however, may show higher than average plasma IgE levels.
• There is a 75% chance that a child with two allergic parents will also develop allergies.
• This number drops to a 30-50% chance if only one of the parents has allergies.
• Various approaches have been taken to identify genes that carry a risk for allergic
diseases, including:
• positional cloning
• candidate gene studies
• Genome-wide association studies.
Based on the known functions of the proteins encoded by many of these genes,
rational speculations can be made about how altered expression or activity of these
proteins might impact the development or severity of allergic diseases. Nonetheless, we
still know very little about whether or not the genetic polymorphisms that are associated
with increased risk for allergy actually alter expression or function of the encoded
proteins, and, in many cases, it is not clear how the function of many of the encoded
proteins could impact the development of allergy.
25. 1- IgE production
1-1- Genetic Susceptibility to Allergic Diseases
• One of the first significant findings from genetic studies of allergy was the identification of a susceptibility locus for atopy on chromosome 5q, near
the site of the gene cluster encoding the cytokines IL-4, IL-5, IL-9, and IL-13 and the IL-4 receptor. This region is of great interest because of the
connection between several genes located there and the mechanisms of IgE regulation and mast cell and eosinophil growth and differentiation.
• Among the genes in this cluster, polymorphisms in the IL-33 gene appear to have the strongest association with asthma. The loci containing genes
encoding IL33, a component of the IL33 receptor (IL1R1) and the transcription factor RORα is required for ILC2 differentiation.
ADAM33, disintegrin and metalloprotease domain 33; DPP10, dipeptidyl peptidase like 10; FcεRI, Fcε receptor type I; Ig, immunoglobulin; ILCs, innate lymphoid cells; MHC, major histocompatibility
complex; ORMDL3, orosomucoid like 3; PHF11, plant homeodomain finger protein 11; TLR, toll-like receptors.
26. 1- IgE production
1-1- Genetic Susceptibility to Allergic Diseases
• Mutations that result in loss of expression or function of the protein filaggrin
(filament aggregating protein) result in significant risk for development of
atopic dermatitis in early childhood, and subsequent allergic diseases
including asthma. Filaggrin is required for skin barrier functions and water
retention, and a lack of this protein is thought to promote keratinocyte
damage and cytokine release, as well as allergen entry into the dermis.
• Some genes whose products regulate the innate immune response to infections include
• CD14, a component of the lipopolysaccharide receptor
• TLR2
• TLR4
have been associated with allergy and asthma. Because innate responses to many infections generally favor
development of Th1 responses and inhibit Th2 responses , it is possible that polymorphisms or mutations in genes
that result in enhanced or diminished innate responses to common infectious organisms may influence the risk for
development of atopy.
• Other genome-wide association studies have found significant associations of common variants of numerous other
genes with asthma and other atopic diseases. However, either the products of these genes are of unknown function,
or the connection between their known functions and the development of atopic disease is not known
28. 1- IgE production
1-2-The Environmental Factors in Allergy
It is clear that environmental influences have a significant impact on the development of allergy, and they
synergize with genetic risk factors.
Environmental influences include :
• Exposure to allergens themselves (It is an important determinant of the amount of specific IgE
antibodies produced)
• To infectious organisms
• Possibly other factors that impact mucosal barrier function, such as air pollution.
29. 1- IgE production
1-2-The Environmental Factors in Allergy
• Exposure to allergens
− The time of life when exposure to these environmental factors
occurs, especially early-life exposure, appears to be important.
− Repeat exposure to a particular antigen is necessary for development
of an allergic reaction to that antigen because switching to the IgE
isotype and sensitization of mast cells with IgE must happen before
an immediate hypersensitivity reaction to an antigen can occur. A
dramatic example of the importance of repeated exposure to antigen
in allergic disease is seen in cases of bee stings. The proteins in the
insect venoms are not usually of concern on the first encounter
because an atopic individual has no preexisting specific IgE
antibodies. However, IgE may be produced after a single encounter
with antigen with no harmful consequences, and a second sting by an
insect of the same species may induce fatal anaphylaxis! Similarly,
exposures to small amounts of peanuts can trigger fatal reactions in
previously sensitized individuals.
− Individuals with allergic rhinitis or asthma often benefit from a
geographic change of residence with a change in indigenous plant
pollens, although environmental antigens in the new residence may
trigger an eventual return of the symptoms.
30. 1-PRODUCTION OF IgE
1-2-The Environmental Factors in Allergy
• To infectious organisms
− Exposure to microbes during early childhood may reduce the risk for developing allergies. One
possible explanation for the increased prevalence of asthma and other atopic diseases in
industrialized countries is that the frequency of infections in these countries is generally lower. A
variety of epidemiologic data show that early childhood exposure to environmental microbes,
such as those found on farms but not in cities, is associated with decreased prevalence of allergic
disease. Based on these data, the hygiene hypothesis was proposed, which states that early-life
and even perinatal exposure to gut commensals and infections leads to a regulated maturation of
the immune system, and perhaps early development of regulatory T cells. As a result, later in life
these individuals are less likely to mount Th2 responses to noninfectious environmental antigens
and less likely to develop allergic diseases.
− Respiratory viral and bacterial infections are a predisposing factor in the development of asthma
or exacerbations of preexisting asthma. For example, it is estimated that respiratory viral
infections precede up to 80% of asthma attacks in children. This may seem contradictory to the
hygiene hypothesis, but these asthma-associated infections are due to human pathogens that may
damage pulmonary mucosal barriers, while the data supporting the hygiene hypothesis focus on
exposure to a broad range of environmental bacteria not necessarily related to tissue injury.
− Some epidemiologic studies indicate that a failure to colonize the respiratory or GI tract early in
life by particular commensal microbes can increase the risk for respiratory viral infections that
induce asthma.
31. • Stefanie Gilles , Cezmi Akdis , Roger Lauener, Peter Schmid-
Grendelmeier , Thomas Bieber, Georg Schäppi , Claudia Traidl-
Hoffmann .The role of environmental factors in allergy: A critical
reappraisal. Exp Dermatol . 2018 Nov;27(11):1193-1200. doi:
10.1111/exd.13769.
Allergies are complex diseases, influenced by genetic, environmental
and life style factors. Many genes can confer susceptibility to allergic
diseases. Typically, the contribution of each single gene is small, and
several genes contribute to the disease phenotype (gene‐gene
interactions).
Environmental and life style factors modify the expression of genes
via epigenetic mechanisms, which means these modifiers can affect
subsequent generations (environment‐gene interactions).
Priming of the innate immune system occurs by shaping the
composition of the body′s microbiota. This, in turn, affects immune
homoeostasis and predisposition to hypersensitivity in the adult
(environment‐host interaction). Finally, anthropogenic pollutants and
climate change‐related factors exert their negative effects on human
health either directly, by enhancing tissue inflammation and increasing
comorbidities (environment‐host interactions), or indirectly, by
modifying allergen carriers, as shown for pollen‐producing plants
(environment‐environment interactions)
1- IgE production
1-2-The Environmental Factors in Allergy
Possibly other factors that impact mucosal barrier function, such as air pollution.
32. 1- IgE production
1-2-The Environmental Factors in Allergy
Possibly other factors that impact mucosal barrier function, such as air pollution.
34. 1- IgE production
1-3-The nature of the antigens
• The ability of an antigen to trigger allergic reactions may
also be related to its chemical nature.
• Although no structural characteristics of proteins can
definitively predict whether they will be allergenic, some
features are typical of many common allergens. These
include
• Stability
• Glycosylation, Anaphylactic responses to foods are
typically induced by highly glycosylated small proteins.
These structural features probably protect the
antigens from denaturation and degradation in the
gastrointestinal tract and allow them to be absorbed
intact.
• Solubility in body fluids.
35. 1- IgE production
1-3-The nature of the antigens
• Low to medium molecular weight (5 to 70 kD)
• Enzymatic activity. Curiously, many allergens, such as the
• cysteine protease of the house dust mite
• and phospholipase A2 (PLA2) in bee venom,
are enzymes, but the importance of the enzymatic activity to their role as allergens is not known.
The terminology of specific allergens is based on the first three letters of the genus (i.e. Der), followed by the first letter of the species
(i.e. Der p) and the order in which the allergen was purified and defined, i.e. Der_p1 or Fel_d_1 or Bet_v_1. The size of the particles is
important, because it affects howmuch becomes airborne and where it is deposited in the respiratory tract.
36. 1- IgE production
1-3-The nature of the antigens
• Antigens that elicit immediate hypersensitivity reactions
(allergens) are proteins . Because immediate
hypersensitivity reactions are dependent on CD4+ T cells,
T cell–independent antigens, such as polysaccharides,
cannot elicit these reactions unless they become attached
to proteins.
• Some non-protein substances, such as penicillin, can
elicit strong IgE responses. These molecules react
chemically with amino acid residues in self proteins to
form hapten-carrier conjugates, which induce IL-4–
producing helper T cell responses and IgE production
• It is not known why some antigens induce IL-4–producing
helper T cell responses and allergic reactions whereas
others do not. Two important characteristics of allergens
are :
• individuals are exposed to them repeatedly
• unlike microbes, they do not generally stimulate
the types of innate immune responses that are
associated with macrophage and dendritic cell
secretion of Th1- and Th17-inducing cytokines
• Typical allergens include proteins in pollen ,animal
dander, foods ….(Table)
39. 1- IgE production
1-3-The nature of the antigens
• Allergens interact with various parts of the innate immune
system which plays a fundamental role in shaping adaptive
immune responses . The innate immune system
comprises several cell types that express pattern
recognition receptors (PRRs).PRRs recognize pathogen- or
damage-associated molecular patterns (PAMPs, DAMPs)
which frequently accompany allergens.
• Toll-like receptors (TLR) are a conserved family of PRRs.
The allergens
• Der p 2 (house-dust mite)
• Fel d 1 (cat)
• Can f 6 (dog)
bind lipopolysaccharide (LPS) and interact with TLR4,
shifting the LPS-response curve to a Th2-inducing range.
Moreover, Bacterial contaminations present on pollen,
shown for yegrass and Parietaria, are responsible for
triggering TLR2, TLR4 and TLR9 signaling
Dermatophagoides pteronyssinus. A typical house dust mite measures
0.2–0.3 mm in length.House dust mite faecal pellets range from 10 to
40 µm.
40. 1- IgE production
1-3-The nature of the antigens
• NOD-like receptors (NLRs) sense cytoplasmic PAMPs
and DAMPs. Some NLRs are core components of
inflammasomes, protein complexes involved in
generating the pro-inflammatory cytokines IL-1β and
IL-18. Inflammasomes are triggered by
• Der p 1, Api m 4 (bee),
• Ambrosia artemisiifolia pollen extracts.
• C-type lectin receptors contain carbohydrate
recognition domains that bind glycosylated allergens
and trigger pathways that determine T-cell polarization.
They interact with:
• C-type lectin receptor DC SIGN: Ara h 1 (peanut),
Der p 1 (house dust mite) and Can f 1 (dog)
• The mannose receptor :Ara h 1, Der p 1 and 2, Fel
d 1, Can f 1 and Bla g 2 (cockroach)
• Protease-activated receptors (PARs) signal in response
to extracelluar proteases. Allergens of
• house dust mite (Der p 1, -3, -9)
• nd mold (Pen c 13)
activate PAR-2 and induce IL-25 and thymic stromal
lymphopoietin (TSLP).
41. 1- IgE production
1-3-The nature of the antigens
• Surfactant associated proteins (SP), found in the alveoli of the lungs, bind inhaled glycosylated allergens via a carbohydrate
recognition domain. Der p 1 and Der f 1 (house dust mite) degrade SP-A resulting in increased degranulation of mast cells
and basophils triggered by these allergens.
Epithelial cells function as physical barrier whose tight junction proteins are degraded by proteases including Der p 1 and Act d 1 (kiwi). Following allergen contact, epithelial cells
produce TSLP, IL-25 and IL-33 and instruct dendritic cells to induce Th2 responses. Dendritic cells bridge innate and adaptive immunity and polarize the T helper cell response.
Polarization towards a Th2 response in dendritic cell-T cell co-cultures has been shown for Bet v 1 (birch pollen) and Pru p 3 (peach) when cells were derived from allergic donors.
Invariant natural killer T cells (iNKTs) recognize lipids presented by CD1d . They secrete IL-4, -5 and -13 when presented lipids from Brazil nut or sphingomyelin from milk. Co-delivery
of certain lipids and potentially allergenic proteins determine the outcome of the sensitization process.
43. 1- IgE production
1-4- Activation of Type 2 Cytokine–Producing Helper T Cells
• As we mentioned
• Genetic Susceptibility : There is a strong genetic propensity to make Th2 responses against some allergens,
but this alone cannot explain why atopic individuals are prone to developing such responses..
• The Environmental Factors: The signals that drive the differentiation of naive CD4+ T cells into Th2 cells in
response to most environmental antigens are not known.
• The nature of the antigens
may be influenced on the development of allergic disease begins with the differentiation of IL-4–, IL-5–, and IL-13–
producing CD4+ helper T cells in lymphoid tissues .
44. 1- IgE production
1-4- Activation of Type 2 Cytokine–Producing Helper T Cells
• In some chronic allergic diseases, an initiating event may be
epithelial barrier injury, which results in local production of
Th2-inducing cytokines. For instance,
• In a chronic allergic reaction of the skin (atopic
dermatitis), the epithelial injury is usually not visible and
of unknown cause, but sometimes it is related to inherited
deficiency of the keratinocyte protein filaggrin. If the
injury results in increased permeability to water and
solutes, it also increases the absorption of allergens.
• In the bronchial tree of the lung, viral infections are
considered a major cause of the initial injury.
• In both tissues, the epithelial cells are induced to secrete
•IL-25,
•IL-33,
•Thymic stromal lymphopoietin (TSLP).
45. 1- IgE production
1-4- Activation of Type 2 Cytokine–Producing Helper T Cells
• Dendritic cells exposed to cytokines, secreted by
epithelial cells including
•IL-25,
•IL-33,
•Thymic stromal lymphopoietin (TSLP).
are mobilized to migrate to lymph nodes
• DC drive differentiation of naive T cells in the
lymph nodes toward IL-4–, IL-5–, and IL-13–
producing
• Th2
• Tfh cells.
•IL-25, IL-33, and TSLP also activate type 2 ILCs to
upregulate GATA3, which enhances their
transcription and secretion of IL-5 and IL-13.
•The differentiated Th2 cells migrate to tissue sites of
allergen exposure, where they contribute to the
inflammatory phase of allergic reactions.
46. 1- IgE production
1-4- Activation of B Cells and Switching to IgE
• Tfh cells remain in lymphoid organs, where they help B cells. B cells specific for allergens are activated by
Tfh cells in secondary lymphoid organs, as in other T cell–dependent B cell responses .
• In response to
• CD40 ligand
• and cytokines, mainly IL-4 and possibly IL-13, produced by these helper T cells,
the B cells undergo heavy chain isotype switching and produce IgE.
47. 1- IgE production
1-4- Activation of B Cells and Switching to IgE
• IgE circulates as a bivalent antibody and is normally present
in plasma at a concentration of less than 1 μg/mL.
• In pathologic conditions such as
• helminthic infections
• and severe atopy,
this level can rise to more than 1000 μg/mL.
• Allergen-specific IgE produced by plasmablasts and plasma
cells enters the circulation and binds to Fc receptors on
• tissue mast cells
• and circulating basophils ,
so that these cells are sensitized and poised to react to a
subsequent encounter with the allergen.
49. 2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
50. • The major effector cells of immediate
hypersensitivity reactions and allergic
disease by sereting Type 2 cytokine are:
• Th2 cells and possibly ILC2s
• Th2 cells and ILCs contribute to
inflammation by secreting
cytokines.
• Other cells (Mast cells, Basophils ,
and Eosinophils )
• have cytoplasmic granules that
contain preformed amines and
enzymes,
• produce lipid mediators and
cytokines that induce
inflammation.
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
51. • Th2 cells secrete cytokines, including:
• IL-4 induces expression of endothelial VCAM-1, which
promotes the recruitment of eosinophils and
additional Th2 cells into tissues
• IL-5 Activates eosinophils
• IL-13 Stimulates epithelial cells (e.g., in the airways) to
secrete increased amounts of mucus, and excessive
mucus production is also a common feature of these
reactions) that work in combination with mast cells,
eosinophils, and ILCs to promote inflammatory
responses to allergens within tissues.
• Th2 cells also contribute to the inflammation of the late-
phase reaction.
• Consistent with a central role of Th2 cells in immediate
hypersensitivity, more allergen-specific IL-4–secreting T
cells are found in the blood of atopic individuals than in
nonatopic persons. In atopic patients, the allergen
specific T cells also produce more IL-4 per cell than in
normal individuals.
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
2-1-1-Role of Th2 Cells in Allergic Disease
52. • Type 2 ILCs produce many of the
same cytokines as Th2 cells,
specifically
• IL-5
• IL-13
and therefore may have similar
roles in allergic reactions.
• Because ILCs normally reside in
tissues, their cytokines may
contribute to early allergic
inflammation before Th2 cells are
generated and migrate to the
tissues.
• The type 2 ILCs may also work in
concert with Th2 cells later, to
sustain inflammation.
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
2-1-1-Role of ILCs in Allergic Disease
53. • Mast cells, basophils, and eosinophils are myeloid cells that share
some features but differ phenotypically and functionally in
significant ways.
• All mast cells are derived from progenitors in the bone marrow.
Progenitors migrate to the peripheral tissues as immature cells
and undergo differentiation in response to local biochemical
cues, including stem-cell factor released by tissue cells, which
binds to the c-Kit receptor on the mast cell precursors.
• Mature mast cells are found throughout the body, predominantly
• near blood vessels (Fig.)
• nerves
• beneath epithelia.
• They are also present in lymphoid organs.
• Normally, mature mast cells are not found in the circulation.
• Human mast cells vary in shape and have round nuclei, and
the cytoplasm contains membrane-bound granules and lipid
bodies. Morphology of mast cells, basophils, and eosinophils.
Photomicrographs of Wright-Giemsa–stained
perivascular dermal mast cells ( arrows),
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
2-1-2-Properties of Mast Cells, Basophils and eosinophils
54. • In rodents, two main types of mast cell have been recognized:
• those in the intestinal mucosa
• those in the peritoneum and other connective tissue sites
They differ in a number of respects, for example in the type of protease and proteoglycan in their granules, and in
their ability to proliferate and differentiate in response to stimulation by IL‐3 (Table).
• The two types have common precursors and are interconvertible depending upon the environmental conditions,
with the mucosal MCt (tryptase) phenotype favored by IL‐3 and connective tissue MCtc (both tryptase and
chymase) being promoted by relatively high levels of stem cell factor (c‐kit ligand).
•
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
2-1-2-1-Properties of Mast Cells
55. • In humans, two major subsets of mast cells have been described,
• Mucosal mast cells (tryptase‐only positive) have abundant
chondroitin sulfate and tryptase, and little histamine, in their
granules, and are found mostly in
• (gastrointestinal) intestinal mucosa
• respiratory tracts ( alveolar spaces in the lung).
• Connective tissues mast cells have abundant heparin and
neutral proteases (chymase, and carboxypeptidase ) in their
granules, produce large quantities of histamine, and are
found in
• the skin,
• intestinal submucosa
• other connective tissues
• A third, less frequent, population is chymase‐only positive
and is found in the nasal mucosa and intestinal submucosa
However, it is possible that all mast cells can have these
properties, with some quantitative variations, and these are not
features of stable and distinct subsets..
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
2-1-2-1-Properties of Mast Cells
56. • Activated mast cells secrete a variety of mediators that are responsible for the manifestations of allergic
reactions (Table ).
• These include substances that are stored in granules and rapidly released upon activation and others that are
synthesized upon activation and secreted.
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
2-1-2-1-Properties of Mast Cells
57. • Basophils are blood granulocytes with structural and functional similarities to mast cells.Basophils are derived from bone
marrow progenitors (which are different from the precursors of mast cells), mature in the bone marrow, and circulate in
the blood which constitute less than 1% of blood leukocytes.
• Basophils contain granules that bind basic dyes & they are capable of synthesizing many of the same mediators as mast
cells .
• Like mast cells, basophils express Fcε receptor type I (FcεRI), bind IgE, and can be triggered by antigen binding to the IgE.
• Although they are normally not present in tissues, basophils may be recruited to some inflammatory sites such as the skin,
nose, lungs or gut . A basophil degranulating 4 minutes after adding allergen. The degranulation that releases histamine
occurs by fusion of the granule membrane with the external membrane of the cell.
2- IgE binding to cells receptors
2-1-Cells Involved In Allergic Reactions
2-1-2-2-Properties of Basophils
58. 2- IgE binding to cells receptors
2-2-Binding of IgE to Mast Cells and Basophils: the Fcε Receptor
59. • Mast cells, and their circulating counterpart the
basophil, abundantly display the FcεRI high‐affinity (Ka
1010 M−1) receptor for IgE.
• Therefore, at the normal serum concentration of IgE,
although low in comparison to other Ig isotypes (<5 ×
10−10 M), there is full occupancy of FcεRI receptors by
IgE, and the majority of mast cells are always coated
with IgE, even in non-atopic individuals
• The receptor is also expressed, albeit at considerably
lower levels, on
• Langerhans cells,
• dendritic cells,
• monocytes,
• macrophages,
• neutrophils,
• eosinophils,
• platelets,
• and the intestinal epithelium.
2- IgE binding to cells receptors
2-2-Binding of IgE to Mast Cells and Basophils: the Fcε Receptor
60. • On basophils and mast cells the receptor is a tetramer
consisting of
• an α chain,
• a tetraspan β chain
• and two disulfide‐linked γ chains,
whereas on other cell types, where the receptor is
involved in antigen presentation rather than triggering
degranulation, the β chain is absent and therefore the
receptor is a trimer.
• The α chain possesses two external Ig‐type domains
responsible for binding the Cε3 region of IgE,
• whereas the γ chains and β chain each contain a
cytoplasmic immunoreceptor tyrosine‐based activation
motif (ITAM) for cell signaling.
2- IgE binding to cells receptors
2-2-Binding of IgE to Mast Cells and Basophils: the Fcε Receptor
61. • FcεRI expression on the surface of mast cells and basophils
is increased by IgE, thereby providing a mechanism for the
amplification of IgE-mediated reactions.
• The importance of FcεRI in IgE-mediated immediate
hypersensitivity reactions has been demonstrated in FcεRI
α chain knockout mice. When these mice are given
intravenous injections of IgE specific for a known antigen
followed by that antigen, anaphylaxis does not develop or
is mild, whereas it is a severe reaction in wild-type mice
treated in the same way.
• Another IgE receptor called FcεRII (also known as CD23) is
a protein related to C-type mammalian lectins whose
affinity for IgE is much lower than that of FcεRI.
• The biologic role of FcεRII is not known. CD23 has been
implicated as a negative regulator of IgE and IgG antibody
responses.
Polypeptide chain structure of the highaffinity IgE Fc receptor
(FcεRI). IgE binds to the Ig-like domains of the α chain. The β
chain and the γ chains mediate signal transduction.
The ITAMs in the cytoplasmic region of the β and γ chains are
similar to those found in the T cell receptor complex.
Lyn and Syk are tyrosine kinases that bind to the β and γ chains and
participate in signaling events.
2- IgE binding to cells receptors
2-2-Binding of IgE to Mast Cells and Basophils: the Fcε Receptor
63. 3- Re-exposure and Activation of Mast Cells
3-1- Activation of Mast Cells
• in non-atopic individuals, the IgE molecules bound to mast
cells are specific for cross-linking are similar to the proximal
signaling events initiated by antigen binding to lymphocytes .
• In contrast, In an individual allergic to a particular antigen, a
large proportion of the IgE bound to FcεRI on the surface of
mast cells is specific for that antigen. Exposure to the antigen
will cross-link sufficient IgE molecules to trigger mast cell
activation.
• When FcεR1 is cross-linked by an allergen via bound IgE, Lyn
tyrosine kinase that is constitutively associated with the
cytoplasmic tail of the FcεRI β chain phosphorylates the
nearby ITAMs in the cytoplasmic tails of FcεRI β and γ chains.
• The tyrosine kinase Syk is then recruited to the ITAMs of the
γ chain, becomes activated, and phosphorylates and
activates other proteins in the signaling cascade, including
several adaptor molecules and enzymes that participate in
the formation of multicomponent signaling complexes.
FIGURE Mast cell activation. Antigen binding to IgE cross-links FcεRI
molecules on mast cells, which induces the release of mediators that cause the
hypersensitivity reaction (A and B). Other stimuli, including the complement
fragment C5a, can also activate mast cells
64. • The complex includes phospholipase Cγ (PLCγ), which catalyzes phosphatidylinositol bisphosphate (PIP2)breakdown to
yield inositol trisphosphate (IP3) and diacylglycerol (DAG), which in turn generate Ca++ and protein kinase C (PKC) signals,
respectively. PKC is also activated in mast cells by PI3-kinase. These signaling events lead to three major responses ,
including ,
• Degranulation
• Lipid mediator production
• Cytokine production.
3- Re-exposure and Activation of Mast Cells
3-1- Activation of Mast Cells
65. • Activated PKC (protein kinase C )
phosphorylates the myosin light chain
component of actin-myosin complexes
located beneath the plasma membrane,
leading to disassembly of the complex.
• This allows cytoplasmic granules to
come in contact with the plasma
membrane.
• The mast cell granule membrane then
fuses with the plasma membrane, a
process that is mediated by members of
the SNARE protein family, which are
involved in many other membrane fusion
events.
3- Re-exposure and Activation of Mast Cells
3-1-1- Activation of Mast Cells: Degranulation
66. • Different SNARE proteins present on the granule and plasma membranes
interact to form a multimeric complex that catalyzes fusion. The formation
of SNARE complexes is regulated by several accessory molecules,
including:
• Rab3 guanosine triphosphatases
• Rab-associated kinases and phosphatases.
• In resting mast cells, these enzymes inhibit mast cell granule
membrane fusion with the plasma membrane.
• But, on FcεRI cross-linking, the resulting increase in
cytoplasmic calcium concentrations and the activation of PKC
block the activity of the inhibitory accessory molecules.
• In addition, calcium sensor proteins respond to the elevated calcium
concentrations by promoting SNARE complex formation and membrane
fusion.
• Following membrane fusion, the contents of the mast cell granules are
released into the extracellular environment.
• This process can occur within seconds of FcεRI cross-linking and can be
visualized morphologically by loss of the dense granules of mast cells .
Mast cell activation. A photomicrograph of a
resting mast cell with abundant purplestaining
cytoplasmic granules is shown in C. These
granules are also seen in the electron micrograph
of a resting mast cell shown in E. In contrast, the
depleted granules of an activated mast cell are
shown in the photomicrograph (D) and electron
micrograph (F).
3- Re-exposure and Activation of Mast Cells
3-1-1- Activation of Mast Cells: Degranulation
67. Aggregation of the FcεRI α chains in lipid rafts leads to ITAMs in the β and γ chains of the receptor interacting with the Lyn, Syk, and Fyn protein tyrosine kinases.
Phosphorylation of Spleen tyrosine kinase (Syk) leads to its activation and it in turn phosphorylates and activates the membrane adaptor LAT1 and LAT2 (NTAL)
proteins that recruit phospholipase Cγ1 (PLCγ1) and adaptor molecules concerned in the activation of GTPase/kinase cascades.
Activation of PLCγ1 generates diacylglycerol (DAG) that targets protein kinase C, while inositol 1,4,5‐triphosphate (IP3) elevates cytoplasmic Ca2+ by depleting the
ER stores. The raised calcium concentration activates transcriptional factors and causes granule exocytosis.The Grb‐2/Sos and Slp‐76/Vav complexes associate with the
LAT1 adaptor, and Grb‐2/Sos additionally with LAT2, and trigger the Ras GTPase‐induced serial kinase cascade leading to the activation of transcription factors and
rearrangements of the actin cytoskeleton.
68. • Synthesis of lipid mediators is
controlled by the cytosolic enzyme
phospholipase A2 (PLA2).
• This enzyme is activated by two signals:
• Elevated cytoplasmic Ca++
• Phosphorylation catalyzed by a
mitogen-activated protein (MAP)
kinase, such as extracellular
receptor-activated kinase (ERK).
ERK is activated as a consequence
of a kinase cascade initiated
through the receptor ITAMs.
• Once activated, PLA2 hydrolyzes
membrane phospholipids to release
arachidonic acid, which is converted by
• Cyclooxygenase
• Lipoxygenase
into different mediators.
3- Re-exposure and Activation of Mast Cells
3-1-2- Activation of Mast Cells: Lipid mediator production
69. • Cytokine secretion by activated mast cells
is a consequence of newly induced
cytokine gene transcription.
• Recruitment and activation of various
adaptor molecules and kinases in
response to FcεRI cross-linking lead to
nuclear translocation of
• nuclear factor of activated T cells
(NFAT)
• nuclear factor κB (NF- κB)
• activation of activation protein 1
(AP-1)
by protein kinases such as c-Jun N-
terminal kinase.
• These transcription factors stimulate
expression of several cytokines such as:
• IL-4
• IL-5
• IL-6
• IL-13
• Tumor necrosis factor [TNF]
3- Re-exposure and Activation of Mast Cells
3-1-3- Activation of Mast Cells: Cytokine production
70. 3- Re-exposure and Activation of Mast Cells
3-2- other inflammatory stimuli of activated mast cell
71. • In addition, to allergen-induced cross-linking of FcεRI, many other
inflammatory stimuli can activate mast cells in the absence of allergens or
synergize with allergens.
• The complement fragments C3a and C5a can cause mast cell
degranulation, and this is why they were named anaphylatoxins.
• Cold temperatures and intense exercise also trigger mast cell
degranulation, but the mechanisms involved are not known.
• Other stimuli induce selective activation of mast cells to produce
arachidonic acid metabolites, cytokines, and chemokines, but not
degranulation. These stimuli include :
• Toll-like receptor (TLR) ligands;
• substances released from injured cells
• fungal glucans
• antimicrobial peptides
• cytokines such as SCF, IL-3, IL-4, IL-9, and IL-33
• ATP
• Leukotrienes
• several chemokines
These additional modes of mast cell activation may be important in non–
immune-mediated immediate hypersensitivity reactions, or they may
amplify IgE-mediated reactions.
3- Re-exposure and Activation of Mast Cells
3-2- other inflammatory stimuli of activated mast cell
72. • Many neuropeptides, including
• substance P,
• somatostatin,
• and vasoactive intestinal peptide,
induce mast cell histamine release and may mediate neuroendocrine linked mast cell activation.
• The nervous system is known to modulate immediate hypersensitivity reactions, and neuropeptides may be
involved in this effect. The flare produced at the edge of the wheal in elicited immediate hypersensitivity
reactions is in part mediated by the nervous system, as shown by the observation that it is markedly diminished in
skin sites lacking innervation.
3- Re-exposure and Activation of Mast Cells
3-2- other inflammatory stimuli of activated mast cell
73. • Mast cell degranulation can also be stimulated by
many different cationic substances, collectively
called secretagogues. These include endogenous
inflammatory peptides, drugs known to cause
adverse allergy-like reactions, and the compounds
48/80 and mastoparan used experimentally as
pharmacologic triggers for mast cells. Most of these
agents contain a shared tetrahydroisoquinoline
motif, and they work by activating a G protein–
coupled receptor.
• Mast cells also express Fc receptors for IgG heavy
chains, and the cells can be activated by cross-
linking bound IgG. This IgG-mediated reaction is the
likely explanation for the finding that Ig ε chain
knockout mice are not completely resistant to
antigen-induced mast cell–mediated anaphylaxis.
However, IgE is the major antibody isotype involved
in most immediate hypersensitivity reactions.
• Mast cell activation is not an all-or-nothing
phenomenon, and different types or levels of
stimuli may elicit partial responses, with production
of some mediators but not others. Such variations
in activation and mediator release may account for
variable clinical presentations.
3- Re-exposure and Activation of Mast Cells
3-2- other inflammatory stimuli of activated mast cell
74. 3- Re-exposure and Activation of Mast Cells
3-3- Inhibition of activated mast cell
75. • Mast cell activation through the FcεRI pathway is
regulated by various inhibitory receptors, which
contain immunoreceptor tyrosine-based inhibition
motifs (ITIMs) within their cytoplasmic tails.
• One such inhibitory receptor is FcγRIIB, which co-
aggregates with FcεRI during mast cell activation.
• The ITIM of FcγRIIB is phosphorylated by Lyn, and
this leads to recruitment of the phosphatase called
SH2 domain–containing inositol 5-phosphatase (SHIP)
and inhibition of FcεRI signaling.
• Several other inhibitory receptors are also expressed
on mast cells, but their importance in vivo is not yet
known.
3- Re-exposure and Activation of Mast Cells
3-3- Inhibition of activated mast cell
77. • The effector functions of mast cells are mediated by soluble molecules released from the activated cells .
• These mediators may be divided into:
• Preformed mediators, which include
• vasoactive amines
• granule macromolecules
• Newly synthesized mediators, which include
• lipid mediators
• cytokines
4- Activation of Cells
4-1- Mediators Derived From Mast Cells
78. • Many of the biologic effects of mast cell activation are
mediated by vasoactive amines that are released from
cytoplasmic granules and act on blood vessels and smooth
muscle.
• Vasoactive amines are low–molecular-weight compounds
that contain an amine group and act directly on blood
vessels.
• In human mast cells, the major mediator of this class is
histamine, but in some rodents, serotonin may be of equal
or greater import.
• Histamine acts by binding to target cell receptors, and
different cell types express distinct classes of histamine
receptors (e.g., H1, H2, H3) that can be distinguished by
their sensitivity to different pharmacologic inhibitors.
• The actions of histamine are short-lived because histamine
is rapidly removed from the extracellular milieu by amine-
specific transport systems.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-1- Preformed mediators
4-1-1-1-Vasoactive Amines
79. • On binding to cellular receptors, histamine initiates intracellular events, such as phosphatidylinositol
breakdown to IP3 and DAG, and these products cause different changes in different cell types.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-1- Preformed mediators
4-1-1-1-Vasoactive Amines
80. • Binding of histamine to endothelium causes contraction of the
endothelial cells, leading to
• increased interendothelial spaces
• increased vascular permeability
• leakage of plasma into the tissues.
• Histamine also stimulates endothelial cells to synthesize
vascular smooth muscle cell relaxants, such as :
• prostacyclin (PGI2)
• nitric oxide,
which cause vasodilation.
• These actions of histamine produce the wheal-and-flare
response of immediate hypersensitivity .
• H1 receptor antagonists (commonly called antihistamines) can inhibit
the vascular responses to intradermal allergen or anti-IgE antibody.
• Figure :The wheal-and-flare reaction in the skin and allergy skin tests. A, In a clinical
test for allergies, different antigens are introduced into the skin by short needles.
Patients with allergies to an antigen will have antigen-specific IgE already bound to
mast cells in the skin and the mast cells will be activated. In response to antigen-
stimulated release of mast cell mediators, local blood vessels first dilate and then
become leaky to fluid and macromolecules, which produces redness and local
swelling (a wheal). Subsequent dilation of vessels on the edge of the swelling
produces the appearance of a red rim (the flare). B, Photograph of a typical allergy
positive skin test showing wheal-and-flare reactions in the skin in response to
injection of allergens
4-1- Mediators Derived From Mast Cells
4-1-1- Preformed mediators
4-1-1-1--Vasoactive Amines
81. • Histamine also causes contraction of
• intestinal smooth muscle
• bronchial smooth muscle.
Thus, histamine may contribute to the
increased peristalsis and bronchospasm
associated with ingested and inhaled
allergens, respectively.
• However, in some allergic disorders, and
especially in asthma, antihistamines are not
effective at suppressing the reaction.
• Moreover, bronchoconstriction in asthma is
more prolonged than are the effects of
histamine, indicating that other mast cell–
derived mediators are important in some
forms of immediate hypersensitivity.
FIGURE 20.6 Biologic effects of mediators of immediate hypersensitivity. Mast cells
and basophil mediators include vasoactive amines and enzymes stored preformed in granules, as
well as cytokines and lipid mediators, which are largely newly synthesized on cell activation. The
biogenic amines and lipid mediators induce vascular leakage, bronchoconstriction, and intestinal
hypermotility, all components
of the immediate response. Cytokines and lipid mediators contribute to inflammation, which is
part of the
late-phase reaction. Enzymes probably contribute to tissue damage. Activated eosinophils release
preformed
cationic proteins as well as enzymes that are toxic to parasites and host cells. Some eosinophil
granule
enzymes probably contribute to tissue damage in chronic allergic diseases.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-1- Preformed mediators
4-1-1-1-Vasoactive Amines
82. • Neutral serine proteases, including
• Tryptase :
• Tryptase is present in all human mast cells and is not known to be present
in any other cell type. Therefore, the presence of tryptase in human
biologic fluids is interpreted as a marker of mast cell activation, and is
sometimes used clinically to diagnose anaphylaxis.
• Tryptase cleaves fibrinogen and activates collagenase, thereby causing
tissue damage.
• Chymase, :
• Chymase is found in some human mast cells, and its presence or absence is
one criterion for characterizing human mast cell subsets, as discussed
earlier.
• Chymase can convert angiotensin I to angiotensin II, which causes
transient vasoconstriction, degrades epidermal basement membranes,
and stimulate mucus secretion.
• Chymase contribute to tissue damage in immediate hypersensitivity
reactions.
• Other enzymes found within mast cell granules include:
• Carboxypeptidase A
• Cathepsin G
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-1- Preformed mediators
4-1-1-2- Granule Enzymes and Proteoglycans
83. • Basophil granules also contain several enzymes, some of which are the
same as those in mast cell granules, such as neutral proteases.
• Other enzymes, such as
• Major basic protein
• Lysophospholipase,
are found in eosinophil but not mast cell granules.
• Proteoglycans, including
• Heparin
• Chondroitin sulfate
are also major constituents of mast cell granules.These molecules
are composed of a polypeptide core and multiple unbranched
glycosaminoglycan side chains that impart a strong net negative
charge to the molecules. Within the granules, proteoglycans serve as
storage matrices for positively charged amines, proteases, and other
mediators and prevent their accessibility to the rest of the cell.
• The mediators are released from the proteoglycans at different rates
after granule exocytosis, with vasoactive amines dissociating much
more rapidly than tryptase or chymase. In this way, the proteoglycans
may control the kinetics of immediate hypersensitivity reactions.
4- 4-1- Mediators Derived From Cells
4-1-1- Preformed mediators
4-1-1-2- Granule Enzymes and Proteoglycans
84. • Mast cell activation results in the rapid de
novo synthesis and release of lipid mediators
that have a variety of effects on
• blood vessels,
• bronchial smooth muscle,
• leukocytes.
• The most important of these mediators are
derived from arachidonic acid, which is
generated by PLA2-mediated hydrolysis of
membrane phospholipids, as discussed
earlier.
• Arachidonic acid is then metabolized by
• Cyclooxygenase pathways
• Lipoxygenase pathways
to produce mediators of allergic reactions.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-2- Newly synthesized mediators
4-1-2-1-Lipid Mediators
85. • The major arachidonic acid–derived
mediator produced by the
cyclooxygenase pathway in mast cells is
prostaglandin D2 (PGD2). Released PGD2
binds to receptors on smooth muscle
cells and acts as a
• vasodilator
• broncho- constrictor
• Promotes neutrophil chemotaxis
and accumulation at inflammatory
sites.
• PGD2 synthesis can be prevented by
cyclooxygenase inhibitors, such as aspirin
and other nonsteroidal antiinflammatory
agents. These drugs may paradoxically
exacerbate asthmatic
bronchoconstriction because they shunt
arachidonic acid toward production of
leukotrienes.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-2- Newly synthesized mediators
4-1-2-1-1- Lipid Mediators :the cyclooxygenase pathway
86. • The major arachidonic acid–derived mediators produced by the
lipoxygenase pathway are the leukotrienes, especially LTC4 and its
degradation products LTD4 and LTE4, all of which are called
cysteinyl leukotrienes.
• LTC4 is made by mucosal mast cells and basophils, but not by
connective tissue mast cells.
• Mast cell–derived leukotrienes bind to specific receptors on
smooth muscle cells, different from the receptors for PGD2, and
cause prolonged bronchoconstriction.
• Collectively, the cysteinyl leukotrienes constitute what was once
called slow reacting substance of anaphylaxis (SRS-A) and are now
known to be important mediators of asthmatic
bronchoconstriction.
• When injected into the skin, these leukotrienes produce a long-
lived wheal-and-flare reaction.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-2- Newly synthesized mediators
4-1-2-1-2- Lipid Mediators : the lipoxygenase pathway
87. • A third type of lipid mediator produced by mast cells and basophils,
as well as several other cell types, is platelet-activating factor (PAF),
named for its discovery as an inducer of rabbit platelet aggregation.
PAF is synthesized as a derivative of membrane phospholipids.
• It has direct bronchoconstricting actions, causes retraction of
endothelial cells, and relaxes vascular smooth muscle.
• However, PAF is hydrophobic and is rapidly destroyed by a plasma
enzyme called PAF hydrolase, which limits its biologic actions.
• Individuals with an inherited deficiency of PAF hydrolase are
at high risk for developing early onset asthma.
• Levels of PAF and its metabolites are elevated in anaphylaxis.
• In rodent models, pharmacologic inhibitors of PAF receptors
ameliorate some aspects of immediate hypersensitivity in the
lung, but PAF antagonists have not proven useful in clinical
trials.
• PAF may also be important in late-phase reactions, in which it
can activate inflammatory leukocytes.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-2- Newly synthesized mediators
4-1-2-1-3- Lipid Mediators : the lipoxygenase pathway
88. • Many different cytokines that contribute to allergic inflammation (the
late-phase reaction) produce by Mast cells include:
• Cytokines ( TNF, IL-1, IL-4, IL-5, IL-6, IL-9, IL-13, CCL3, CCL4)
• Colony-stimulating factors ( IL-3 , granulocyte-macrophage colony-
stimulating factor (GM-CSF)
• Mast cell activation induces transcription and synthesis of these cytokines,
but preformed TNF may also be stored in granules and rapidly released on
FcεRI crosslinking.
• Th2 cells that are recruited into the sites of allergic reactions also produce
some of these cytokines.
• The cytokines that are released from activated mast cells, Th2 cells, and
possibly ILCs are mainly responsible for the inflammation associated with
the late-phase reaction.
• TNF activates endothelial expression of adhesion molecules and together
with chemokines accounts for neutrophil and monocyte infiltrates.
• In addition to allergic inflammation, mast cell cytokines also contribute to
innate immune responses to infections. For example, mouse models
indicate that mast cells are required for effective defense against some
bacterial infections, and this effector function is mediated largely by TNF.
4- Activation of Mast Cells
4-1- Mediators Derived From Mast Cells
4-1-2- Newly synthesized mediators
4-1-2-2- Cytokines
89. • Eosinophils are bone marrow–derived
granulocytes that are abundant in the
inflammatory infiltrates of late phase reactions
and are involved in many of the pathologic
processes in allergic diseases.
• GM-CSF, IL-3, and IL-5 promote eosinophil
differentiation from myeloid precursors in the
bone marrow, and after maturation they circulate
in the blood.
• Eosinophils are normally present in peripheral
tissues, especially in mucosal linings of the
respiratory, gastrointestinal, and genitourinary
tracts, and their numbers can increase by
recruitment in the setting of inflammation.
• The granules of eosinophils contain basic proteins
that bind acidic dyes such as eosin .
4- Activation of Cells
4-2-Properties of Eosinophils
90. • Cytokines produced by Th2 cells promote the activation of eosinophils and their recruitment to late-phase reaction sites.
Th2 cells and type 2 ILCs are sources of IL-5.
• IL-5 is a potent eosinophil-activating cytokine that enhances the ability of eosinophils to release granule contents. In the
absence of this cytokine (e.g., in IL-5 knockout mice), there is a deficiency of eosinophil numbers and functions.
4- Activation of Cells
4-2-Properties of Eosinophils
91. • Eosinophils are recruited into late-phase reaction sites, as
well as sites of helminthic infection, and their recruitment is
mediated by a combination of adhesion molecule
interactions and chemokines.
• Eosinophils bind to endothelial cells expressing
• E-selectin
• VCAM-1,
the ligand for the VLA-4 integrin.
• IL-4 produced by Th2 cells may enhance expression of
adhesion molecules for eosinophils.
• Eosinophil recruitment and infiltration into tissues also
depend on the chemokine eotaxin (CCL11), which is
produced by epithelial cells at sites of allergic reactions and
binds to the chemokine receptor CCR3, which is expressed
constitutively by eosinophils.
• In addition, other chemoattractants for eosinophils are:
• the complement product C5a
• the lipid mediators PAF and LTB4 produced by mast
cells
4- Activation of Cells
4-2-Properties of Eosinophils
92. • Upon activation, eosinophils release granule proteins that
are toxic to microbes and may injure normal tissues.The
granule contents of eosinophils include
• lysosomal hydrolases found in other granulocytes
• as well as eosinophil-specific proteins that are
particularly toxic to helminthic organisms, including
• major basic protein
• and eosinophil cationic protein.
These two cationic polypeptides have no known
enzymatic activities, but they are toxic to
helminths and bacteria, as well as normal
tissue.
• In addition, eosinophilic granules contain eosinophil
peroxidase, which is distinct from the myeloperoxidase
found in neutrophils and catalyzes the production of
hypochlorous or hypobromous acid. These products are also
toxic to helminths, protozoa, and host cells.
• Activated eosinophils, like mast cells and basophils, produce
and release lipid mediators, including PAF, prostaglandins,
and the cysteinyl leukotrienes. These eosinophil-derived
lipid mediators may contribute to the pathologic processes
of allergic diseases.
• Eosinophils also produce a variety of cytokines that may
promote inflammatory responses and tissue repair, but the
biologic significance of eosinophil cytokine production is not
known.
4- Activation of Cells
4-2-Properties of Eosinophils
95. 5- IgE- & Mast Cell– Dependent Reactions
• The cells and mediators we have discussed are
responsible for the
• immediate vascular changes :
• The immediate vascular and smooth
muscle reaction to allergen develops
within minutes after challenge (allergen
exposure in a previously sensitized
individual
• The immediate reaction is characterized
by (B)
− Vasodilation
− congestion
− edema,
• late-phase reactions :
• The late-phase reaction develops 2 to 24
hours later
• The late-phase reaction is characterized
by an inflammatory infiltrate rich in
eosinophils, neutrophils, and T cells. (C)
96. 5- IgE- AND Mast Cell– Dependent Reactions
5-1- The Immediate Reaction
• The early vascular changes that occur during immediate
hypersensitivity reactions are demonstrated by the wheal
and- flare reaction to the intradermal injection of an
allergen (Fig. ).
• When an individual who has previously encountered an
allergen and produced IgE antibody is challenged by
intradermal injection of the same antigen, the injection
site becomes red from locally dilated blood vessels
engorged with red blood cells. The site then rapidly swells
as a result of leakage of plasma from the venules.
• This soft swelling is called a wheal and can involve an area
of skin as large as several centimeters in diameter.
• Subsequently, blood vessels at the margins of the wheal
dilate and become engorged with red blood cells,
producing a characteristic red rim called a flare.
• The full wheal-and-flare reaction can appear within 5 to
10 minutes after administration of antigen and usually
subsides in less than 1 hour.
The wheal-and-flare reaction in the skin and allergy skin tests. A, In a
clinical test for allergies, different antigens are introduced into the skin
by short needles. Patients with allergies to an antigen will have
antigen-specific IgE already bound to mast cells in the skin and the
mast cells will be activated. In response to antigen-stimulated release
of mast cell mediators, local blood vessels first dilate and then become
leaky to fluid and macromolecules, which produces redness and local
swelling (a wheal). Subsequent dilation of vessels on the edge of the
swelling produces the appearance of a red rim (the flare). B,
Photograph of a typical allergy positive skin test showing wheal-and-
flare reactions in the skin in response to injection of allergens.
(Courtesy of Dr. David Sloane, Department of Medicine, Brigham and
Women’s Hospital, Boston, MA.)
97. 5- IgE- AND Mast Cell– Dependent Reactions
5-1- The Immediate Reaction
• The wheal-and-flare reaction is dependent on IgE and mast cells. A causal
association of IgE and mast cells with immediate hypersensitivity was first
deduced from experiments involving the passive transfer of IgE antibodies from an
allergic individual into a normal recipient. For example, immediate hypersensitivity
reactions against an allergen can be elicited in unresponsive individuals if the local
skin site is first injected with IgE from an allergic individual. Such adoptive transfer
experiments were first performed with serum from immunized individuals, and
the serum factor responsible for the reaction was originally called reagin. For this
reason, IgE molecules are still sometimes called reaginic antibodies. The antigen-
initiated skin reaction that follows adoptive transfer of IgE is called passive
cutaneous anaphylaxis.
• The wheal-and-flare reaction results from sensitization of dermal mast cells by IgE
binding to FcεRI, crosslinking of IgE by the antigen, and activation of mast cells
with release of mediators, notably histamine.
• Histamine binds to histamine receptors on venular endothelial cells; the
endothelial cells synthesize and release
• PGI2
• and nitric oxide,
and these mediators cause vasodilation and vascular leak, as described earlier.
• Skin mast cells appear to produce only small amounts of long-acting mediators
such as leukotrienes, so the wheal-and-flare response subsides rapidly. Allergists
often test patients for allergies to different antigens by examining the ability of
these antigens applied in skin patches or administered through small needle pricks
to elicit wheal-and-flare reactions.
98. 5- IgE- AND Mast Cell– Dependent Reactions
5-2- The Late-Phase Reaction
• The immediate wheal-and-flare reaction is followed 2 to 4
hours later by a late-phase reaction consisting of the
accumulation of inflammatory leukocytes, including
neutrophils, eosinophils, basophils, and helper T cells.
• The inflammation is maximal by about 24 hours and then
gradually subsides.
• Cytokines produced by mast cells, including TNF, upregulate
endothelial expression of leukocyte adhesion molecules, such
as E-selectin and intercellular adhesion molecule 1 (ICAM-1),
and chemokines, which results in the recruitment of blood
leukocytes .Thus, mast cell activation promotes the influx of
leukocytes into tissues. The types of leukocytes that are
typical of late-phase reactions are eosinophils and helper T
cells.
• Although Th2 cells are the dominant T cell population in
uncomplicated late-phase reactions, the cellular infiltrates in
chronic atopic dermatitis and asthma contain Th1 and Th17
cells, as well as T cells that produce both IL-17 and IFNγ.
Neutrophils are also often present in these reactions.
• Eosinophils and Th2 cells both express CCR4 and CCR3, and
the chemokines that bind to these receptors are produced by
many cell types at sites of immediate hypersensitivity
reactions, including epithelial cells.
99. 5- IgE- AND Mast Cell– Dependent Reactions
5-2- The Late-Phase Reaction
• The late-phase reaction may occur without a detectable preceding immediate hypersensitivity reaction.
• Bronchial asthma is a disease in which there may be repeated bouts of inflammation with accumulations of eosinophils
and Th2 cells without the vascular changes that are characteristic of the immediate response.
• In such disorders, there may be little mast cell activation, and the cytokines that sustain the late-phase reaction may be
produced mainly by T cells. Chronic inflammation of the asthmatic airway results in altered structure referred to as remodeling.
100. 5-2-1- Chronic inflammation :Remodeling
• Chronic inflammation of the asthmatic airway results in altered
structure referred to as remodeling. The epithelium, extra-
cellular matrix and smooth muscle layers are involved to a
variable degree in all patients with chronic asthma.
• Epithelial injury with loss of cilia, vacuolated cells, and,
ultimately, shedding of cells results in loss of the integrity of
this diffusion barrier, increasing permeability of the airway
mucosa.
• Increased airway mucus from hypertrophic submucosal glands
and hyperplastic goblet cells contributes to an increased
surface tension favoring airway closure.Also contributing to
the overall increase in asthmatic airway wall thickness are
deposition of reticulin, fibronectin, and collagens I and III in
the lamina reticularis and the extracellular matrix beyond the
reticular basement membrane (BM). Bronchial vessels
adjacent to the epithelial BM proliferate and are dilated.
Endothelial gaps and exudation of plasma hasten epithelial
shedding.
• Finally, hypertrophy and hyperplasia of airway smooth
muscle, occupying up to 20% of the bronchial wall, contribute
to the airway luminal narrowing observed in chronic
asthma.People with asthma have trouble getting air out, the
airways are blocked. They have no trouble getting air in.
People with asthma will down in their own secretions. The
secretions are very sticky and glue like.
103. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
• The manifestations of allergic diseases depend on
• the tissues in which the mast cell mediators and type 2 cytokines have effects,
• as well as the chronicity of the resulting inflammatory process.
• Atopic individuals may have one or more types of allergy, the most common forms being : Allergic rhinitis
,Bronchial asthma, Atopic dermatitis …
104. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
• The clinical and pathologic features of allergic reactions vary with
the anatomic site of the reaction, for several reasons.
• The point of contact with the allergen can determine the
organs or tissues where mast cells and Th2 cells are
activated. For example,
• inhaled antigens cause rhinitis or asthma,
• ingested antigens often cause vomiting and diarrhea
(but can also produce skin and respiratory symptoms if
larger doses are ingested),
• injected antigens cause systemic effects on the
circulation.
• The concentration of mast cells in various target organs
influences the severity of responses. For example,
• Mast cells are particularly abundant in the skin and the
mucosa of the respiratory and gastrointestinal tracts,
and these tissues frequently suffer the most injury in
immediate hypersensitivity reactions.
• The local mast cell phenotype may influence the
characteristics of the immediate hypersensitivity reaction.
For example,
• connective tissue mast cells produce abundant
histamine and are responsible for wheal-and-flare
reactions in the skin.
105. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma
Normal bronchus a patient with asthma
106. • Asthma includes a group of pulmonary diseases characterized by recurrent reversible airflow obstruction and
bronchial smooth muscle cell hyperresponsiveness, which is most often caused by repeated immediate-type
hypersensitivity and late-phase allergic reactions (Fig)
Histopathologic features of bronchial asthma. Atopic bronchial asthma results from repeated immediate hypersensitivity reactions in the lungs with chronic late-
phase reactions. A crosssection of a normal bronchus (A) and a cross-section of a bronchus from a patient with asthma (B) are shown. The diseased bronchus has
excessive mucus (M) production, many submucosal inflammatory cells (including eosinophils), and smooth muscle (SM) hypertrophy, and many more goblet cells
than in the normal bronchus (black arrows in insets).
Normal bronchus a patient with asthma
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma
107. • Asthma affects approximately 20 million people in the
United States, and the frequency of this disease has
increased significantly over the past 30 to 40 years. The
prevalence rate is similar to that in other industrialized
countries, but it may be lower in less developed areas of
the world.
• Patients suffer paroxysms of bronchoconstriction and
increased production of thick mucus, which lead to
bronchial obstruction and respiratory difficulties.
• Asthma in adults frequently coexists with chronic
obstructive pulmonary disease, and the combination of
these diseases can cause severe irreversible airflow
obstruction. Affected individuals may suffer considerable
morbidity, and asthma can be fatal.
• Approximately 70% of cases of asthma are associated
with IgE-mediated reactions reflecting atopy.
• In the remaining 30% of patients, asthma may be
triggered by nonimmune stimuli, such as drugs, cold, and
exercise. Even among nonatopic asthmatics, the
pathophysiologic process of airway constriction is similar,
which suggests that alternative mechanisms of mast cell
degranulation (e.g., by locally produced
neurotransmitters) may underlie the disease.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma
108. • The pathophysiologic sequence in atopic asthma is
probably initiated by mast cell activation in
response to allergen binding to IgE, as well as by
Th2 cells reacting to allergens .
• The lipid mediators and cytokines produced by the
mast cells and T cells lead to the recruitment of
eosinophils, basophils, and more Th2 cells.
• The chronic inflammation in this disease may
continue without mast cell activation. There is
experimental evidence that other T cell subsets,
including Th1 and Th17 cells, and IL-9–secreting T
cells contribute to the pathology of established
disease.
• Smooth muscle cell hypertrophy and
hyperreactivity are thought to result from
leukocyte-derived mediators and cytokines. Mast
cells, basophils, and eosinophils all produce
mediators that constrict airway smooth muscle.
The most important of the bronchoconstricting
mediators are cysteinyl leukotrienes.
• Increased mucus secretion results from the action
of cytokines, mainly IL-13, on bronchial epithelial
cells
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma
109. • The major mediators of acute bronchoconstrictionare thought to
beMast cell–derived
• leukotrienes
• and platelet activating factor (PAF)
• Therapy is targeted at
• reducing mast cell activation with anti–immunoglobulin E (IgE)
• mast cell degranulation with inhibitors such as cromolyn
• and at countering mediator actions on bronchial smooth muscle
by
• leukotriene antagonists :Leukotriene receptor antagonists
block the binding of bronchocon stricting leukotrienes to
receptors on airway smooth muscle cells.
• bronchodilators : Bronchial smooth muscle cell relaxation
is achieved principally by drugs that elevate intracellular
cyclic adenosine monophosphate (cAMP) levels in smooth
muscle cells, which inhibits contraction. The major drugs
used to elevate cAMP are activators of adenylate cyclase,
including inhaled longacting β2-adrenergic agonists.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma Mediators and treatment of asthma
110. • Mast cell–derived cytokines are thought to be the major
mediators of sustained airway inflammation, which is an example
of a late-phase reaction;
• Inhaled corticosteroids block the production of
inflammatory cytokines. Corticosteroids may also be given
systemically, especially once an attack is under way, to
reduce inflammation.
• antibodies are used block the actions of the cytokines.
• Humanized monoclonal anti-IgE.
• Humanized monoclonal anti-IL-5 (mepolizumab and
reslizumab) and an anti-IL-5 receptor antibody
(benralizumab).
• Monoclonal antibodies against IL-4Rα. IL-4Rα is a
subunit common to both the IL-4 and IL-13.
• Because histamine has little role in airway constriction,
antihistamines (H1 receptor antagonists) are not useful in the
treatment of asthma. Indeed, because many antihistamines are
also anticholinergics, these drugs may worsen airway obstruction
by causing thickening of mucus secretions.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma Mediators and treatment of asthma
111. • Humanized monoclonal anti-IgE. Humanized monoclonal
anti-IgE treatment can reduce sensitivity and significantly
decrease the number of acute episodes of asthma per year.
• Antibodies directed against the binding site for FcεRI on IgE
bind to IgE in the circulation, but not when it is attached to
mast cells or basophils. An antibody of this kind can
therefore remove IgE from the circulation but will not induce
anaphylaxis.
• A mouse monoclonal antibody to IgE has been progressively
humanized so that the molecule can be safely injected into
patients and will bind IgE with high affinity. Treatment of
chronic urticaria with anti-IgE has also been established.
Although the treatment is approved without IgE assays, it is
more consistently effective in patients with higher levels of
IgE. Treatment with anti-IgE antibodies has reduced
exacerbations of asthma and the symptoms of hay fever. In
addition, continued treatment that controls free IgE below
10 ng/mL leads to a progressive decrease in the number of
IgE receptors on mast cells. Thus, the treatment may achieve
a secondary effect, further decreasing the sensitivity of
histamine-containing cells to allergen. Although there have
been small studies on the role of anti-IgE in treating food
allergy, atopic dermatitis, urticaria and drug allergy, this
treatment remains to be established
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma Mediators and treatment of asthma
112. • An anti-IL-5 monoclonal antibody is approved for
severe asthma refractory to other treatments.
Humanized monoclonal anti-IL-5 and anti-IL-5
receptor antibodies.
• A recent Cochrane Database Systematic Review
examined 13 studies that enrolled 6000 patients
that included randomized trials comparing
− IL-5 neutralizing antibodies (mepolizumab
and reslizumab)
− and an anti-IL-5 receptor antibody
(benralizumab)
versus placebo in adults and children with
eosinophilic asthma refractory to existing
treatments. In this review, all three of the anti-IL-
5 treatments reduced rates of clinically significant
asthma exacerbation by approximately 50% in
participants with severe eosinophilic asthma on
standard of care with poorly controlled disease
who had experienced two or more exacerbations
in the preceding year or an increased
requirement for medication. This report
supported the concept that IL-5 was a critical
mediator in asthma characterized by eosinophilia.
• .
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma Mediators and treatment of asthma
113. • Monoclonal antibodies against IL-4Rα. IL-4Rα is a
subunit common to both the IL-4 and IL-13.
• Inhibiting signalling through the IL-4Rα subunit
thus blocks the activity of both IL-4 and IL-13,
critical cytokines in allergic inflammatory
responses. Monoclonal antibodies to IL-4Rα have
been used to investigate the effect of blocking IL-
4 and IL-13 signalling on asthma pathogenesis.
Treatment with dupilumab, a fully human
monoclonal antibody to IL-4Rα, reduced asthma
exacerbations when long-acting β-agonists and
inhaled glucocorticoids were withdrawn in
patients with persistent, moderate-to-severe
asthma and increased blood eosinophilia. In
addition, the antibody improved lung function
and blunted TH2-associated inflammatory
markers. It also increased lung function and
decreased severe exacerbations in patients with
uncontrolled persistent asthma, irrespective of
baseline blood eosinophil number. These results
suggest that the combination of blocking IL-4 and
IL-13 signalling through the IL- 4Rα may be a
robust strategy to inhibit TH2 inflammatory
responses.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-1- Bronchial Asthma Mediators and treatment of asthma
114. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-2- Immediate Hypersensitivity Reactions in the Upper Respiratory Tract
115. • Allergic rhinitis, also called hay fever, is perhaps the most
prevalent allergic disease and is a consequence of immediate
hypersensitivity reactions to common allergens such as plant pollen
or house dust mites localized to the upper respiratory tract by
inhalation.
• The pathologic and clinical manifestations include
• mucosal edema,
• leukocyte infiltration with abundant eosinophils,
• mucus secretion,
• coughing,
• sneezing
• difficulty in breathing.
• Allergic conjunctivitis with itchy eyes is commonly associated
with the rhinitis.
• Focal protrusions of the nasal mucosa, called nasal polyps,
filled with edema fluid and eosinophils may develop in
patients who suffer frequent repetitive bouts of allergic
rhinitis.
• Antihistamines are commonly used to treat allergic rhinitis.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-2- Immediate Hypersensitivity Reactions in the Upper Respiratory Tract
116. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-3-Immediate Hypersensitivity Reactions in the gastrointestinal Tract
117. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-3-Immediate Hypersensitivity Reactions in the gastrointestinal Tract
• Food allergies are immediate hypersensitivity
reactions to ingested foods that lead to the release of
mediators from intestinal mucosal and submucosal
mast cells of the gastrointestinal tract, including the
oropharynx.
• The resulting clinical manifestations include
• pruritus,
• tissue edema,
• enhanced peristalsis,
• increased epithelial fluid secretion,
• and symptoms of oropharyngeal
swelling,vomiting, and diarrhea.
Rhinitis, urticaria, and mild bronchospasm are also
often associated with allergic reactions to food,
suggestive of systemic antigen exposure, and
anaphylaxis may occasionally occur. Individuals
may be sufficiently sensitive to these allergens
that severe systemic reactions can occur in
response to small accidental ingestions.
• Allergies to foods, including cow’s milk, eggs,
peanuts, tree nuts, shellfish, fish, soy, and wheat, are
extremely common across the world.
Food allergy. Harald Renz, Katrina J. Allen, Scott H. Sicherer, Hugh A.
Sampson, Gideon Lack, , Kirsten Beyer & Hans C. Oettgen . Nature
Reviews Disease Primers volume 4, Article number: 17098
(2018)doi:10.1038/nrdp.2017.98 impact factor of 21.506
118. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
119. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
• Common allergic reactions in the skin include
• Urticaria
• Atopic dermatitis
120. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
6-4-1- Urticaria
• Urticaria, or hives, is an acute wheal-and-flare reaction induced by mast
cell mediators and occurs in response to direct local contact with an
allergen or after an allergen enters the circulation.
• It is triggered by a wide variety of antigens or by physical stimuli, including
• cold,
• pressure,
• and sunlight .
• Urticaria is a spectrum ranging
• from simple wheals
• to angioedema. Angioedema Usually localised (to head & neck) but
may be more generalised urticaria. Presents as swelling of the face,
neck and oropharynx. Represents mast cell degranulation in skin
deep to dermis vs. superficial dermis in urticaria.
• Clinical distinction between
• Acute urticaria
• Chronic urticaria : It is defined by recurrence over a period of 6 weeks
or more and is often of unknown cause
is important for diagnosis and treatment.
Extensive urticaria.
Angioedema
121. • Urticaria can be caused by
• immunologic (autoimmune, IgE–dependent, immune complex–mediated,
complement-kinin dependent)
• non-immunologic (direct mast cell–releasing agents, vasoactive stimuli,
drugs).
• Local degranulation of mast cells with the release of histamine and other
factors, such as slow-reacting substance of anaphylaxis, precipitates urticaria.
• Functional IgG autoantibodies, which release histamine from mast cells and
basophils, are commonly found in the blood of patients with chronic urticaria.
• Additionally, basophils are recruited into the wheals, where they sustain the
response by releasing histamine.
• Eosinophils also contribute through leukotriene C 4 (LTC 4 ), LTD 4 , LTE 4 , and
major basic protein.
• Urticaria is typically transient and self-limited, without leakage of blood cells
into the skin or damage to the blood vessels. Leakage of plasma into the dermis
from capillaries and small postcapillary venules correlates clinically with
development of a demarcated, pink, raised lesion (hive).
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
6-4-1- Urticaria Pathobiology
Dermatographic urticaria -
122.
2. Medications based on severity
o
A. Mild to moderate, acute urticaria
• Oral H 1 -antihistamines, e.g., diphenhydramine (Benadryl), 10-50 mg PO q12h, or hydroxyzine, 10-25 mg PO q8h;
nonsedating alternatives include cetirizine (Zyrtec), 5-10 mg/day, or loratadine (Claritin), 10 mg/day
• Omalizumab (an anti-IgE antibody) at 300 mg subcutaneously monthly
o
B. Severe urticaria with or without angioedema
• H 1 -antihistamines, e.g., diphenhydramine (Benadryl), 25-50 mg PO q6-8h or 10-50 mg IV q2-4h, not to exceed
400 mg/24 hr
• Corticosteroids, e.g., prednisone, 10-60 mg PO every morning with tapering over a 2-wk period; triamcinolone
(Kenalog), 40 mg IM for one dose; or dexamethasone, 0.6-0.75 mg/m 2 /day IV in divided doses q6-12h, depending
on severity
o
D. Chronic idiopathic urticaria—combination therapy
• Nonsedating H 1 -antihistamine: cetirizine, 10 mg/day, or fexofenadine, 30-180 mg twice daily, alone or with
montelukast, 10 mg/day, or H 1 and H 2 antagonists (50 mg each of diphenhydramine and ranitidine) and/or low-dose
corticosteroids (if unavoidable)
• Omalizumab (anti-IgE monoclonal antibody) at 300 mg subcutaneously once monthly and cycloclosporine (doses of
3 mg/kg or higher for 8 to 16 weeks)
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
6-4-1- Urticaria
123. • Atopic dermatitis (commonly called eczema) is
part of the atopic triad :
• atopic dermatitis
• allergic rhinitis
• asthma: Some patients with atopic
dermatitis develop asthma, a sequence that
clinicians refer to as the “atopic march.”
but can also occur in isolation.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
6-4-2- Atopic dermatitis
124. • in a chronic allergic reaction of the skin called atopic dermatitis,
the epithelial barrier abnormality is usually not visible and of
unknown cause, but sometimes it is related to inherited
deficiency of filaggrin, which is a keratinocyte protein needed to
maintain normal barrier function of the skin.
• injury induces epithelial cells to secrete
• IL-25,
• IL-33
• , and thymic stromal lymphopoietin (TSLP).
• DCs exposed to these cytokines are mobilized to migrate to
lymph nodes and to drive differentiation of naive T cells in the
lymph nodes toward IL-4–, IL-5–, and IL-13–producing Th2 and
Tfh cells.
• IL-25, IL-33, and TSLP also activate ILC2s to upregulate GATA3,
which enhances the transcription and secretion of IL-5 and IL-
13. Thus, epithelial cell–derived cytokines may provide a link
between allergens and type 2 responses.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
6-4-2- Atopic dermatitis
125. • Eczema patients go on to develop chronic late-phase reactions in the skin. As may be expected for a cytokine-mediated
response, the late-phase inflammatory reaction is not inhibited by antihistamines but can be treated with corticosteroids,
which inhibit cytokine synthesis.
• An antibody to the shared subunit of the IL-4 and IL-13 receptors has been shown to be effective in clinical trials in a subset
of patients with atopic dermatitis.
• Eosinophil major basic protein in the skin of atopic dermatitis. Skin biopsy from a patient with severe atopic dermatitis. The
haematoxylin and eosin (H&E) stain shows an inflammatory infiltrate, but very few intact eosinophils are present. The same section
stained with antibodies to eosinophil major basic protein (MBP) shows extensive deposition of MBPin the dermis, demonstrating
that eosinophils had degranulated in the skin.
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
6-4-2- Atopic dermatitis
126. Infantile atopic dermatitis is one of the most troublesome skin
eruptions in children. There is an “atopic” background without a
clearly defined immunologic pattern.
Physical Examination: Skin Lesions TYPE Red skin, tiny vesicles on
“puffy” surface, scaling, exudation with wet crusts, and cracks
DISTRIBUTION Regional, especially the face (sparing the mouth),
the antecubital and popliteal fossae, wrists, and lateral aspects of
the legs
Management: Prompt response to potent corticosteroids; after
improvement, use low-potency corticosteroids or emollients.
Laboratory and Special Examinations: Culture Rule out
secondary Staphylococcus aureus or herpes simplex virus (HSV)
infection.
Radioallergosorbent Testing (RAST) For Specific Allergens 90%
clinically relevant if negative, but only 20% clinically relevant if
positive
Patch Test Usually noncontributory. In patients who have IgE
antibodies to house dust mites, pollens, or animal hair proteins,
patch tests to these allergens may be positive
6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-4-Immediate Hypersensitivity Reactions in the Skin
6-4-2- Infantile atopic dermatitis
128. 6- Allergic Diseases in Humans: Pathogenesis & Therapy
6-5- Systemic Anaphylaxis
• The World Allergy Organisation
Anaphylaxis Committee defines
anaphylaxis as:
• A serious systemic
hypersensitivity reaction that is
usually rapid in onset and may
cause death.
• Organs involved in Anaphylaxis:
• skin and mucous membranes,
• upper and lower respiratory
tract,
• gastrointestinal tract,
• cardiovascular system,
• central nervous system.
• Clinical Manifestations of Anaphylaxis
(fig)