The document outlines the materials and steps needed to perform hybridization in lab conditions using sweet pea flowers of different varieties. The key steps are: 1) emasculating female flowers by removing their anthers, 2) collecting pollen from male flowers by crushing their anthers, and 3) dusting the collected pollen onto the stigma of the emasculated female flowers. The hybridized flowers are then covered with paper bags to avoid contamination from external pollen sources.

1. Materials required to study hybridization in lab conditions tile Flowers of sweet pea of different verities Butter Paper bag

2. Open the flower with the help of needle

3. Remove the anther - emasculation Pg Anthers

4. Now in this flower only pistil is left-female flower Anther

5. Place it on the tile and label it as female flower Female flower

6. Male flower, open it with the help of needle

7. Crush the anther and take out pollen grains

8. Dust the pollen grains on the stigma of the female flower.

9. Hybridization-Mating between two individuals or lines differing in genotype. In natural conditions flower is intact to the plant.

10. In natural conditions this process is done at the time of bud conditions of flower .

11. Cover the flower with paper bag

12. Display your steps of hybridization Emasculated flower- female Flower-pollens are collected- male Hybridized flower Covered to avoid any contamination