Human antibody library
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Creative Biolabs is an undisputed world-leading service provider and research institution in phage display library technique. We offer following world-class premade human and mouse antibody libraries with great capacity & diversity that can derive highly specific, high affinity antibodies ranging from 10 pM to 10 nM.
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Antibody mouse
Creative Biolabs is an undisputed world-leading service provider and research institution in phage display library technique. We offer following world-class premade human and mouse antibody libraries with great capacity & diversity that can derive highly specific, high affinity antibodies ranging from 10 pM to 10 nM.
Transgenic mice service
Since the first EGFR targeting antibody panitumumab (Vectibix) from humanized mice has obtained regulatory approval in 2005, dozens of additional transgenic mouse-derived mAbs have entered human clinical testing, i.e., CD20 (Ofatumumab), CD4 (Zanolimumab), CD70, etc. These genetically "humanized" animals have become one of the most powerful strategies for therapeutic/ diagnostic antibody development, which is now the fastest growing group of mAb drugs in clinical trials currently.
Antibody avidity
We use an error-prone PCR integrated DNA-shuffling approach to mutate mainly CDR regions during sub-library construction. If the potential of introducing immunogenic mutations to framework positions is not a concern, we usually use this approach to create mutations at completely random positions across the entire VH and VL fragments. In these cases, the genetic diversity of the sub-library is further increased via passage through our proprietary bacterial mutator strain, CD-affi™.
Advantages of Immune Library
Advantages of Immune Library
Highly enriched target-specific sdAbs
The most common and standard technique (2-4 months for one specific antigen)
High affinity of obtained binders from nM to pM
https://www.creative-biolabs.com/sdab/construction-of-immune-single-domain-antibody-library.htm
Hybridoma Sequencing Service
Hybridoma sequencing refers to the process of obtaining sequence information regarding the cDNA encoding the variable heavy (VH) and variable light (VL) domains of the antibody produced by your hybridoma cell line.https://www.creative-biolabs.com/antibody-sequencing-service.html
Phage display technology
Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.https://www.creative-biolabs.com/phage-display-service.html
sdAb Screening Services
Thanks to a longstanding presence in the market of sdAb development and our well-established screening platform, Creative Biolabs is the right partner to navigate the sdAb screening challenges. Focused on the field of sdAb discovery and development, we will use our skills to offer innovative and sophisticated sdAb screening services tailored to our customer's exact needs.
Recommended
Antibody mouse
Creative Biolabs is an undisputed world-leading service provider and research institution in phage display library technique. We offer following world-class premade human and mouse antibody libraries with great capacity & diversity that can derive highly specific, high affinity antibodies ranging from 10 pM to 10 nM.
Transgenic mice service
Since the first EGFR targeting antibody panitumumab (Vectibix) from humanized mice has obtained regulatory approval in 2005, dozens of additional transgenic mouse-derived mAbs have entered human clinical testing, i.e., CD20 (Ofatumumab), CD4 (Zanolimumab), CD70, etc. These genetically "humanized" animals have become one of the most powerful strategies for therapeutic/ diagnostic antibody development, which is now the fastest growing group of mAb drugs in clinical trials currently.
Antibody avidity
We use an error-prone PCR integrated DNA-shuffling approach to mutate mainly CDR regions during sub-library construction. If the potential of introducing immunogenic mutations to framework positions is not a concern, we usually use this approach to create mutations at completely random positions across the entire VH and VL fragments. In these cases, the genetic diversity of the sub-library is further increased via passage through our proprietary bacterial mutator strain, CD-affi™.
Advantages of Immune Library
Advantages of Immune Library
Highly enriched target-specific sdAbs
The most common and standard technique (2-4 months for one specific antigen)
High affinity of obtained binders from nM to pM
https://www.creative-biolabs.com/sdab/construction-of-immune-single-domain-antibody-library.htm
Hybridoma Sequencing Service
Hybridoma sequencing refers to the process of obtaining sequence information regarding the cDNA encoding the variable heavy (VH) and variable light (VL) domains of the antibody produced by your hybridoma cell line.https://www.creative-biolabs.com/antibody-sequencing-service.html
Phage display technology
Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.https://www.creative-biolabs.com/phage-display-service.html
sdAb Screening Services
Thanks to a longstanding presence in the market of sdAb development and our well-established screening platform, Creative Biolabs is the right partner to navigate the sdAb screening challenges. Focused on the field of sdAb discovery and development, we will use our skills to offer innovative and sophisticated sdAb screening services tailored to our customer's exact needs.
Phage Display Screening for Single Domain Antibody (sdAb)
Phage display screening（https://www.creative-biolabs.com/sdab/phage-display-screening-for-single-domain-antibody-sdab.htm） is a well-established technique to identify antibody fragments and other binding molecules against any targets of interest. As each clone in phage display libraries represents a noncombinatorial functional domain of a naturally circulating antibody, phage display screening is a powerful method for sdAb discovery. Here, Creative Biolabs describes the phage display screening for sdAb generation.
sdAb Functional Identification
Single domain antibodies (sdAbs, or VHH) are the smallest antigen-binding units of antibodies, comprising either one variable domain or one engineered constant domain that solely facilitates target binding. They are popular as a novel class of proprietary therapeutic proteins containing unique structural and functional properties. At Creative Biolabs, we offer one-stop solutions for sdAb development and characterization to promote the identification of novel therapeutic sdAbs to meet your specific project demands.
https://www.creative-biolabs.com/sdab/sdab-functional-identification.htm
Sdab discovery
Single Domain Antibody also known as domain antibody, VHH, VNARor sdAb, is a kind of antibody fragments consisting of a single monomeric variable antibody domain and lacking the light chain and CH domain of the heavy chain in conventional Fab region.https://www.creative-biolabs.com/sdab/one-stop-solution-for-sdab-development.htm
Magic phage
As alternatives, Creative Biolabs also offers transgenic mouse/rat hybridoma and Native plasma cell sorting strategies which can also incorporate with transgenic mice for antibody development. Our experienced scientists are always willing to discuss with every customer on a case-to-case basis. Please feel free to inquiry us for more details.
One-Stop Antibody Drug Discovery Services from GenScript ProBio
GenScript ProBio offers one-stop antibody drug discovery services from target to preclinical candidates, including lead generation(hybridoma, single B cell and antibody library), and lead Optimization(humanization, affinity maturation)and testing(in vitro/Vivo pharmacology, developability). GenScript ProBio delivers antibody leads with good biological efficacy, safety & manufacturability in 12-15 months. For more information, visit our website today.
OriGene Technologies Capabilities Overview Feb 2011
Opportunity overview with OriGene Technologies. OriGene is looking to identify strategic collaboration partners for its full-length human proteins, validated monoclonal antibodies and "gene centric" tool box.
Gene Libarries
DNA library is a collection of DNA, three types of recombinant DNA libraries are present
1) Genomic library
2) Chromosomal library
3) Complementary DNA library
dkNET Webinar "YCharOS: Antibody Characterization Through Open Science" 10/22...
Abstract
Many (most!) genes/proteins linked to disease phenotypes remain severely understudied [1]. Distinct open-science initiatives are needed to promote the exploration of currently understudied proteins, from the proper identification of research reagents [2], to the development of a chemical probes for every human protein [1]. We focus on the proper characterization of antibodies to guide researchers in selecting the most specific/selective antibodies for their needed application(s) [3].
Antibodies are among the most commonly used reagents in cell biology. Generally, scientists purchase antibodies from commercial suppliers, and rely on the vendor’s quality control data to make their purchasing decisions. While there are many outstanding commercially-available antibodies, many other antibodies do not perform as advertised - and in the absence of an objective means to compare performance, it is impossible to tell one from the other. This is a widely known problem that plagues tens of thousands of scientists annually [4-6].
There is a scientific solution, enabled by CRISPR/Cas9 technology. By comparing signals from wild-type and isogenic knockout cells, one can readily test the specificity of antibodies. We applied this approach in a pilot study demonstrating that only three of the 16 commercially-available antibodies for C9ORF72, the protein product of a major amyotrophic lateral sclerosis disease locus, specifically recognized the protein. Distressingly, neither antibody had been used in a publication, and the antibody used most frequently in publications, which have been cited thousands of times, did not recognize the protein in any application [7].
We are now applying our antibody characterization pipeline to generate head-to-head comparisons of commercial antibodies for all human proteins. This work is performed in partnership with high-quality manufacturers that provide in-kind reagents (i.e. antibodies and knock-out lines). Finalized antibody characterization reports are progressively uploaded on a free open-science repository (https://zenodo.org/communities/ycharos/). We believe our initiative, Antibody Characterization through Open Science (YCharOS), will contribute to make science more reproducible and help illuminate the dark genome.
The top 3 key questions that YCharOS can answer:
1. Do antibodies used in my field perform as advertised?
2. How do I identify the best performing antibody for my protein of interest?
3. Do I need to launch an expensive and time-consuming antibody generation study or do effective commercial antibodies already exist for my protein of interest?
References (see https://dknet.org/about/blog/2334)
Presenter: Carl Laflamme, PhD, Senior Postdoctoral Fellow at the Montreal Neurological Institute (The Neuro, McGill University). Chetan Raina, YCharOS, CEO.
Upcoming webinars schedule: https://dknet.org/about/webinar
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Justin Gaines
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✆ 617.347.0573
☏ 949.722.1950 x 852
✉ jgaines@dvbiologics.com
Antibody development case study
Presented here is a case study in which the client would like
to generate a rabbit monoclonal antibody recognizing a specific epitope on the target protein F1. We constructed an immune rabbit antibody library and performed biopanning with competitive elution.
B cell sorting
The single plasma cell interrogation platform uses single cell-based screening of entire antibody-secreting B cell populations along with next-generation sequencing to retrieve native antibodies with extremely high in vivo specificity and affinity. During the native antibody development, the isolation and separation of single cells are the main technically challenging tasks. The yield and quality or in other words the integrity and purity of the cells as well as the throughput and the sensitivity of single cell isolation methods should be considered.
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Lucatumumab
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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Phage Display Screening for Single Domain Antibody (sdAb)
Phage display screening（https://www.creative-biolabs.com/sdab/phage-display-screening-for-single-domain-antibody-sdab.htm） is a well-established technique to identify antibody fragments and other binding molecules against any targets of interest. As each clone in phage display libraries represents a noncombinatorial functional domain of a naturally circulating antibody, phage display screening is a powerful method for sdAb discovery. Here, Creative Biolabs describes the phage display screening for sdAb generation.
sdAb Functional Identification
Single domain antibodies (sdAbs, or VHH) are the smallest antigen-binding units of antibodies, comprising either one variable domain or one engineered constant domain that solely facilitates target binding. They are popular as a novel class of proprietary therapeutic proteins containing unique structural and functional properties. At Creative Biolabs, we offer one-stop solutions for sdAb development and characterization to promote the identification of novel therapeutic sdAbs to meet your specific project demands.
https://www.creative-biolabs.com/sdab/sdab-functional-identification.htm
Sdab discovery
Single Domain Antibody also known as domain antibody, VHH, VNARor sdAb, is a kind of antibody fragments consisting of a single monomeric variable antibody domain and lacking the light chain and CH domain of the heavy chain in conventional Fab region.https://www.creative-biolabs.com/sdab/one-stop-solution-for-sdab-development.htm
Magic phage
As alternatives, Creative Biolabs also offers transgenic mouse/rat hybridoma and Native plasma cell sorting strategies which can also incorporate with transgenic mice for antibody development. Our experienced scientists are always willing to discuss with every customer on a case-to-case basis. Please feel free to inquiry us for more details.
One-Stop Antibody Drug Discovery Services from GenScript ProBio
GenScript ProBio offers one-stop antibody drug discovery services from target to preclinical candidates, including lead generation(hybridoma, single B cell and antibody library), and lead Optimization(humanization, affinity maturation)and testing(in vitro/Vivo pharmacology, developability). GenScript ProBio delivers antibody leads with good biological efficacy, safety & manufacturability in 12-15 months. For more information, visit our website today.
OriGene Technologies Capabilities Overview Feb 2011
Opportunity overview with OriGene Technologies. OriGene is looking to identify strategic collaboration partners for its full-length human proteins, validated monoclonal antibodies and "gene centric" tool box.
Gene Libarries
DNA library is a collection of DNA, three types of recombinant DNA libraries are present
1) Genomic library
2) Chromosomal library
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dkNET Webinar "YCharOS: Antibody Characterization Through Open Science" 10/22...
Abstract
Many (most!) genes/proteins linked to disease phenotypes remain severely understudied [1]. Distinct open-science initiatives are needed to promote the exploration of currently understudied proteins, from the proper identification of research reagents [2], to the development of a chemical probes for every human protein [1]. We focus on the proper characterization of antibodies to guide researchers in selecting the most specific/selective antibodies for their needed application(s) [3].
Antibodies are among the most commonly used reagents in cell biology. Generally, scientists purchase antibodies from commercial suppliers, and rely on the vendor’s quality control data to make their purchasing decisions. While there are many outstanding commercially-available antibodies, many other antibodies do not perform as advertised - and in the absence of an objective means to compare performance, it is impossible to tell one from the other. This is a widely known problem that plagues tens of thousands of scientists annually [4-6].
There is a scientific solution, enabled by CRISPR/Cas9 technology. By comparing signals from wild-type and isogenic knockout cells, one can readily test the specificity of antibodies. We applied this approach in a pilot study demonstrating that only three of the 16 commercially-available antibodies for C9ORF72, the protein product of a major amyotrophic lateral sclerosis disease locus, specifically recognized the protein. Distressingly, neither antibody had been used in a publication, and the antibody used most frequently in publications, which have been cited thousands of times, did not recognize the protein in any application [7].
We are now applying our antibody characterization pipeline to generate head-to-head comparisons of commercial antibodies for all human proteins. This work is performed in partnership with high-quality manufacturers that provide in-kind reagents (i.e. antibodies and knock-out lines). Finalized antibody characterization reports are progressively uploaded on a free open-science repository (https://zenodo.org/communities/ycharos/). We believe our initiative, Antibody Characterization through Open Science (YCharOS), will contribute to make science more reproducible and help illuminate the dark genome.
The top 3 key questions that YCharOS can answer:
1. Do antibodies used in my field perform as advertised?
2. How do I identify the best performing antibody for my protein of interest?
3. Do I need to launch an expensive and time-consuming antibody generation study or do effective commercial antibodies already exist for my protein of interest?
References (see https://dknet.org/about/blog/2334)
Presenter: Carl Laflamme, PhD, Senior Postdoctoral Fellow at the Montreal Neurological Institute (The Neuro, McGill University). Chetan Raina, YCharOS, CEO.
Upcoming webinars schedule: https://dknet.org/about/webinar
Expression Library Immunization
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DV Biologics Overview
Justin Gaines
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✆ 617.347.0573
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✉ jgaines@dvbiologics.com
Antibody development case study
Presented here is a case study in which the client would like
to generate a rabbit monoclonal antibody recognizing a specific epitope on the target protein F1. We constructed an immune rabbit antibody library and performed biopanning with competitive elution.
B cell sorting
The single plasma cell interrogation platform uses single cell-based screening of entire antibody-secreting B cell populations along with next-generation sequencing to retrieve native antibodies with extremely high in vivo specificity and affinity. During the native antibody development, the isolation and separation of single cells are the main technically challenging tasks. The yield and quality or in other words the integrity and purity of the cells as well as the throughput and the sensitivity of single cell isolation methods should be considered.
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Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
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Human antibody library
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