How does the movement of DNA fragments on the gel and the movement of the ethilisim
troemide in the eel affect the appearance of the DNA fragments?
Solution
Answer:
the movement of DNA happens from a negative charged electrode to positively charged
electrode as the DNA is negatively charged.
By applying the electric field through the agarose gel matrix DNA is seperated on the basis of
size.
Ethidium bromide is a flourescent dye which intercalates into circular DNA and can
sunsequently change the charge, length, as well as the superhelicity of the DNA molecule,
EtBr presence in gel during electrophoresis can affect movement of DNA. Agarose gel
electrophoresis can be used to resolve circular DNA with different supercoiling topology.
as more EtBr binds between the DNA bases, the molecule stiffens and hence mobility is
decreased as compared to same frangment being run without EtBr.
Ethedium bromide is positively charged and runs from the positive electrode towards the
negative electrode. i.e opposite direction of movement of DNA.
hence when the two encounter, the intercalaton happens, leading to flourescence of orange red
colour under UV light..
How does the movement of DNA fragments on the gel and the movemen.pdf
1. How does the movement of DNA fragments on the gel and the movement of the ethilisim
troemide in the eel affect the appearance of the DNA fragments?
Solution
Answer:
the movement of DNA happens from a negative charged electrode to positively charged
electrode as the DNA is negatively charged.
By applying the electric field through the agarose gel matrix DNA is seperated on the basis of
size.
Ethidium bromide is a flourescent dye which intercalates into circular DNA and can
sunsequently change the charge, length, as well as the superhelicity of the DNA molecule,
EtBr presence in gel during electrophoresis can affect movement of DNA. Agarose gel
electrophoresis can be used to resolve circular DNA with different supercoiling topology.
as more EtBr binds between the DNA bases, the molecule stiffens and hence mobility is
decreased as compared to same frangment being run without EtBr.
Ethedium bromide is positively charged and runs from the positive electrode towards the
negative electrode. i.e opposite direction of movement of DNA.
hence when the two encounter, the intercalaton happens, leading to flourescence of orange red
colour under UV light.
