This document discusses a study that investigated the inhibitory effect of methanol extracts from Alpinia galanga rhizomes on the growth of struvite crystals. Struvite crystals were grown using various concentrations of the methanol extract. The crystals were then characterized using Fourier Transform Infrared Spectroscopy. Results showed that increasing concentrations of the extract reduced the weight of struvite crystals formed, demonstrating the extract's potential to inhibit struvite crystal formation and growth. Phytochemical analysis of the extract identified various compounds that may contribute to its anti-urolithiatic properties.
International Journal of Pharmaceutical Science Invention (IJPSI) inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
DOI:10.21276/ijlssr.2016.2.4.23
ABSTRACT- Atorvastatin calcium is a HMG-CoA reductase inhibitor used for the treatment of hyperlipidaemia. It has
oral bioavailability of ≤12 %. It also undergoes high first pass metabolism. It is highly soluble in acidic pH and absorbed
more in the upper part of the gastrointestinal tract. In order, to improve the absorption and its oral bioavailability,
niosomes of Atorvastatin calcium have been formulated and evaluated on different parameters. Four niosomes
formulations of Atorvastatin calcium were successfully developed by modified ether injection technique using nonionic
surfactant i.e. Span 20, Span 40, Tween 20, Tween 40 and cholesterol at different concentrations. Key-words- Atorvastatin calcium, Niosomes, Surfactants, Cholesterol, Modified ether injection method, in-vitro release,
Stability studies
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Optimization and Production of Itaconic Acid from Estuarine Aspergillus terre...BRNSS Publication Hub
Itaconic acid (IA) is an organic acid. It is used in medicine, resins, agriculture, and polymer production. In the present study, sediment sample was collected aseptically from Vellar estuary, Parangipettai, Tamil Nadu, India. About 1.6 × 102 to 6.1 × 103 colony forming units/g density of fungal strains were isolated and screened for IA production. As a result of the tested strains Aspergillus terreus was observed as the most potential strain. Optimization was done at different temperatures (25–45°C), in different pH (5.0–7.0). The impact of salinity on IA production was evaluated using various salinity (5–25 ppt), carbon sources (1% w/v of glucose, sucrose, dextrose, and maltose), nitrogen sources (0.5% sodium nitrate, ammonium nitrate, and potassium nitrate), and cheaper sources (1% w/v molasses, jackfruit waste, wheat bran, and coconut oil cake). As a result optimized culture condition for IA production was 1% w/v of glucose - best carbon source, 1% w/v molasses - best cheaper carbon source, 0.5% of sodium nitrate - best nitrogen source, salinity - 20 ppt, temperature - 40°C, and pH - 5.5 and incubation time – 96 h. Compared to glucose (0.41 mg/ml) production of IA was high when molasses (0.61 mg/ml) was used as carbon source, it is also economically good. Mass scale culture was done using molasses instead of glucose with an optimized parameter. After mass scale culture, IA production was 6.3g/l.
International Journal of Pharmaceutical Science Invention (IJPSI) inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
DOI:10.21276/ijlssr.2016.2.4.23
ABSTRACT- Atorvastatin calcium is a HMG-CoA reductase inhibitor used for the treatment of hyperlipidaemia. It has
oral bioavailability of ≤12 %. It also undergoes high first pass metabolism. It is highly soluble in acidic pH and absorbed
more in the upper part of the gastrointestinal tract. In order, to improve the absorption and its oral bioavailability,
niosomes of Atorvastatin calcium have been formulated and evaluated on different parameters. Four niosomes
formulations of Atorvastatin calcium were successfully developed by modified ether injection technique using nonionic
surfactant i.e. Span 20, Span 40, Tween 20, Tween 40 and cholesterol at different concentrations. Key-words- Atorvastatin calcium, Niosomes, Surfactants, Cholesterol, Modified ether injection method, in-vitro release,
Stability studies
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Optimization and Production of Itaconic Acid from Estuarine Aspergillus terre...BRNSS Publication Hub
Itaconic acid (IA) is an organic acid. It is used in medicine, resins, agriculture, and polymer production. In the present study, sediment sample was collected aseptically from Vellar estuary, Parangipettai, Tamil Nadu, India. About 1.6 × 102 to 6.1 × 103 colony forming units/g density of fungal strains were isolated and screened for IA production. As a result of the tested strains Aspergillus terreus was observed as the most potential strain. Optimization was done at different temperatures (25–45°C), in different pH (5.0–7.0). The impact of salinity on IA production was evaluated using various salinity (5–25 ppt), carbon sources (1% w/v of glucose, sucrose, dextrose, and maltose), nitrogen sources (0.5% sodium nitrate, ammonium nitrate, and potassium nitrate), and cheaper sources (1% w/v molasses, jackfruit waste, wheat bran, and coconut oil cake). As a result optimized culture condition for IA production was 1% w/v of glucose - best carbon source, 1% w/v molasses - best cheaper carbon source, 0.5% of sodium nitrate - best nitrogen source, salinity - 20 ppt, temperature - 40°C, and pH - 5.5 and incubation time – 96 h. Compared to glucose (0.41 mg/ml) production of IA was high when molasses (0.61 mg/ml) was used as carbon source, it is also economically good. Mass scale culture was done using molasses instead of glucose with an optimized parameter. After mass scale culture, IA production was 6.3g/l.
The aim of this study was to measure the effects of the aqueous extract of leaves of Adansonia digitata (AAD) in vitro on calcium oxalate (CaOx) nucleation and aggregation [by spectrophotometric time course measurements of optical density at 620 nm (OD620)]. For measuring calcium oxalate crystallization inhibitor activity agar gel model, and in vivo on experimentally induced CaOx urolithiasis in male Wistar rats. CaOx urolithiasis in rats was induced by intraperitoneal (i.p.) injection of sodium oxalate (NaOx) (7 mg/100 g/day for 7 days). AAD was administered orally (200 mg/kg/day for 7 days). Urine volume, pH, body weight, kidney weight (wet and dry), serum and urine level of creatinine, urea, magnesium (Mg2+), calcium (Ca2+) were evaluated on day 7. In addition, histopathological changes in kidney and oxalate in urine and kidney were evaluated. The results revealed that AAD inhibited the rate of crystal nucleation (SN) and aggregation (SA) and showed inhibitory activity on CaOx crystallization. The histopathological examination of kidneys revealed that AAD significantly reduced the incidence of CaOx crystal deposition. In addition, AAD significantly increased urinary excretion of Mg2+ along with a decrease of oxalate excretion. In conclusion, the aqueous extract contains potent antiurolithiatic substances which warrant further evaluation.
Evaluation of Antiurolithiatic Activity of Canthium dicoccum Ethanolic Extrac...gynomark
Objective:
The aim of the present study was to evaluate the effect of ethanolic extract of Canthium dicoccum on
Gentamicin and Caliculi Producing Diet (CPD) induced urolithasis in rats.
Methods:
Thirty male albino wistarrats were divided randomly into six groups. Group I were treated with normal
saline, Group II were treated with Gentamicin for a period of 7 days, Group III treated with Cystone
(p.o), Group IV,V,VI was treated with plant extract 150, 300,600 mg/kg respectively for 14 days with
gentamicin treatment from day 7 to 14. All the animals were fed with caliculi Producing diet for entire
study period. Later, urine samples were collected at the end of the studyand analysed for renal function
parameters like Creatiinine, BUN, Serum Urea etc. The kidneys of the sacrificed animals were isolated
and sectioned for histopathological studies.
Results:
The rats treated with ethanolic extract of canthiumdicoccum at doses 150,300,600 mg/kg significantly
showed improved effect and prevented urolithasis in rats which is evident in the reduction of creatinine,
Urea and BUN levels when compared to gentamicin treated group. The histopathological studies also
showed less degenerative changes.It also decreased crystal deposition markedly into the renal tubules
in number as well as size and prevented damage to the renal tubules.
Conclusion:
The results demonstrated that the ethanolic extract had significantly reduced the urolithiasis that was
caused by an CPD diet combined with gentamicine injection in rats.
Keywords: Gentamicin, Anti-Urolithiatic activity, Canthiumdicoccum, ethanol.
Urinary stone evaluation in laboratory and clinical significanceSanjeev Mehta
Urinary Stones or Urolithiasis is a common, painful and destructive disease. It has a habit of recurrence. About half of Stones recur with in 5 to 7 years of first episode.
Stone disease tests are very useful to know cause of Stone formation. This is essential for focal prophylactic treatment to prevent recurrance.
Chitosan- nanoparticles of garlic (A. sativum), temu mangga (C. mangga) and Jeringau (A. calamus) was successfully produced with ionic gelation method. (Particle size : 438 nm (120’), Uniformity rate of 0.14 (very good), FTIR results indicate several important functional group.
Water softeners helps in removing hardness of water and make them fit for our daily decisive usages. For our work,
different water sources like surface water (Hebbal Lake), Bore well water (NPS School, Ozone Urbana, Bangalore) and
Corporation water (BWSSB) were selected and their respective hardness as CaCO3 were analysed before and after
treatment with Moringa oleifera seed extract. The seeds of Moringa oleifera, one of the best natural coagulants as per
the previous studies were used in this protocol. In normal water treatment scheme most preferably ion exchange
techniques were used for the removal of hardness, which would likely to be a resin based technology. Also the ion
exchange procedure was completely dependent on industrial resins, which were manufactured by major corporate
concerns (like Lancer, Toyota, Ion Exchange India Ltd, Thermax Ltd, LG etc.), hence incur huge cost. Industrial resins
have Na+
ions attached to the resin beads replaces Ca2+ and Mg2+ ions present in water during the ion exchange process.
The resin beads can be regenerated or recharged again with Na+
ion by NaCl solution once the resin gets exhausted.
Our work persuaded in another way of removing hardness from water by the principle means of adsorption and
conversion of soluble hardness-causing ions to insoluble products by precipitation reactions. Moringa oleifera seed
extracts were prepared and performed jar test to obtain the best required dosage for hardness removal in the selected
water samples. The obtained dosage (mg/l) or ppm of Moringa oleifera was dosed to the selected water samples
through the dosing system present in an existing water treatment system of capacity 2 m3/hr. The removal efficiency
was observed to be between 50 to 60% after passing through the treatment system with Moringa oleifera dosage.
Hence this work can pave way to find a best alternate method for hardness removal water.
Media Optimization for Nattokinase Production from a Marine Isolate at a Shak...Varad Bende
https://www.ijarse.com/images/fullpdf/1505568147_ijarse_515.pdf
Use of animations disturbs the view. For full ppt, drop me a mail at varadbende96@gmail.com
IJPCBS 2012, 2(1), 110-116 Kavya et al. ISSN: 2249-9504
110
INTERNATIONAL JOURNAL OF PHARMACEUTICAL, CHEMICAL AND BIOLOGICAL SCIENCES
Available online at www.ijpcbs.com
ISOLATION AND SCREENING OF STREPTOMYCES SP. FROM
CORINGA MANGROVE SOILS FOR ENZYME PRODUCTION AND
ANTIMICROBIAL ACTIVITY
M. Kavya Deepthi1*, M. Solomon Sudhakar1 and M. Nagalakshmi Devamma2 1Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, 2Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pr Taadmesihln, aInddui,a I.n dia.
Caesalpinia spinosa (Molina) Kuntze, commonly known as tara (Quechua), is a small leguminous tree or thorny shrub native to Peru. C. spinosa is cultivated as a source of tannins based on a galloylated quinic acid structure. This chemical structure has been confirmed also by LC-MS.
The aim of this study was to measure the effects of the aqueous extract of leaves of Adansonia digitata (AAD) in vitro on calcium oxalate (CaOx) nucleation and aggregation [by spectrophotometric time course measurements of optical density at 620 nm (OD620)]. For measuring calcium oxalate crystallization inhibitor activity agar gel model, and in vivo on experimentally induced CaOx urolithiasis in male Wistar rats. CaOx urolithiasis in rats was induced by intraperitoneal (i.p.) injection of sodium oxalate (NaOx) (7 mg/100 g/day for 7 days). AAD was administered orally (200 mg/kg/day for 7 days). Urine volume, pH, body weight, kidney weight (wet and dry), serum and urine level of creatinine, urea, magnesium (Mg2+), calcium (Ca2+) were evaluated on day 7. In addition, histopathological changes in kidney and oxalate in urine and kidney were evaluated. The results revealed that AAD inhibited the rate of crystal nucleation (SN) and aggregation (SA) and showed inhibitory activity on CaOx crystallization. The histopathological examination of kidneys revealed that AAD significantly reduced the incidence of CaOx crystal deposition. In addition, AAD significantly increased urinary excretion of Mg2+ along with a decrease of oxalate excretion. In conclusion, the aqueous extract contains potent antiurolithiatic substances which warrant further evaluation.
Evaluation of Antiurolithiatic Activity of Canthium dicoccum Ethanolic Extrac...gynomark
Objective:
The aim of the present study was to evaluate the effect of ethanolic extract of Canthium dicoccum on
Gentamicin and Caliculi Producing Diet (CPD) induced urolithasis in rats.
Methods:
Thirty male albino wistarrats were divided randomly into six groups. Group I were treated with normal
saline, Group II were treated with Gentamicin for a period of 7 days, Group III treated with Cystone
(p.o), Group IV,V,VI was treated with plant extract 150, 300,600 mg/kg respectively for 14 days with
gentamicin treatment from day 7 to 14. All the animals were fed with caliculi Producing diet for entire
study period. Later, urine samples were collected at the end of the studyand analysed for renal function
parameters like Creatiinine, BUN, Serum Urea etc. The kidneys of the sacrificed animals were isolated
and sectioned for histopathological studies.
Results:
The rats treated with ethanolic extract of canthiumdicoccum at doses 150,300,600 mg/kg significantly
showed improved effect and prevented urolithasis in rats which is evident in the reduction of creatinine,
Urea and BUN levels when compared to gentamicin treated group. The histopathological studies also
showed less degenerative changes.It also decreased crystal deposition markedly into the renal tubules
in number as well as size and prevented damage to the renal tubules.
Conclusion:
The results demonstrated that the ethanolic extract had significantly reduced the urolithiasis that was
caused by an CPD diet combined with gentamicine injection in rats.
Keywords: Gentamicin, Anti-Urolithiatic activity, Canthiumdicoccum, ethanol.
Urinary stone evaluation in laboratory and clinical significanceSanjeev Mehta
Urinary Stones or Urolithiasis is a common, painful and destructive disease. It has a habit of recurrence. About half of Stones recur with in 5 to 7 years of first episode.
Stone disease tests are very useful to know cause of Stone formation. This is essential for focal prophylactic treatment to prevent recurrance.
Chitosan- nanoparticles of garlic (A. sativum), temu mangga (C. mangga) and Jeringau (A. calamus) was successfully produced with ionic gelation method. (Particle size : 438 nm (120’), Uniformity rate of 0.14 (very good), FTIR results indicate several important functional group.
Water softeners helps in removing hardness of water and make them fit for our daily decisive usages. For our work,
different water sources like surface water (Hebbal Lake), Bore well water (NPS School, Ozone Urbana, Bangalore) and
Corporation water (BWSSB) were selected and their respective hardness as CaCO3 were analysed before and after
treatment with Moringa oleifera seed extract. The seeds of Moringa oleifera, one of the best natural coagulants as per
the previous studies were used in this protocol. In normal water treatment scheme most preferably ion exchange
techniques were used for the removal of hardness, which would likely to be a resin based technology. Also the ion
exchange procedure was completely dependent on industrial resins, which were manufactured by major corporate
concerns (like Lancer, Toyota, Ion Exchange India Ltd, Thermax Ltd, LG etc.), hence incur huge cost. Industrial resins
have Na+
ions attached to the resin beads replaces Ca2+ and Mg2+ ions present in water during the ion exchange process.
The resin beads can be regenerated or recharged again with Na+
ion by NaCl solution once the resin gets exhausted.
Our work persuaded in another way of removing hardness from water by the principle means of adsorption and
conversion of soluble hardness-causing ions to insoluble products by precipitation reactions. Moringa oleifera seed
extracts were prepared and performed jar test to obtain the best required dosage for hardness removal in the selected
water samples. The obtained dosage (mg/l) or ppm of Moringa oleifera was dosed to the selected water samples
through the dosing system present in an existing water treatment system of capacity 2 m3/hr. The removal efficiency
was observed to be between 50 to 60% after passing through the treatment system with Moringa oleifera dosage.
Hence this work can pave way to find a best alternate method for hardness removal water.
Media Optimization for Nattokinase Production from a Marine Isolate at a Shak...Varad Bende
https://www.ijarse.com/images/fullpdf/1505568147_ijarse_515.pdf
Use of animations disturbs the view. For full ppt, drop me a mail at varadbende96@gmail.com
IJPCBS 2012, 2(1), 110-116 Kavya et al. ISSN: 2249-9504
110
INTERNATIONAL JOURNAL OF PHARMACEUTICAL, CHEMICAL AND BIOLOGICAL SCIENCES
Available online at www.ijpcbs.com
ISOLATION AND SCREENING OF STREPTOMYCES SP. FROM
CORINGA MANGROVE SOILS FOR ENZYME PRODUCTION AND
ANTIMICROBIAL ACTIVITY
M. Kavya Deepthi1*, M. Solomon Sudhakar1 and M. Nagalakshmi Devamma2 1Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, 2Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pr Taadmesihln, aInddui,a I.n dia.
Caesalpinia spinosa (Molina) Kuntze, commonly known as tara (Quechua), is a small leguminous tree or thorny shrub native to Peru. C. spinosa is cultivated as a source of tannins based on a galloylated quinic acid structure. This chemical structure has been confirmed also by LC-MS.
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More Info:- https://www.imarcgroup.com/veterinary-diagnostics-market
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1. GROWTH CHARACTERIZATION OF
STRUVITE CRYSTALS INFLUENCED
BY ALPINIA GALANGA METHANOL
RHIZOMES EXTRACT
PRESENTED BY:
S.DHASARADHA BAI
I M.SC BIOCHEMISTRY
2. GROWTH CHARACTERIZATION OF STRUVITE CRYSTALS INFLUENCED BY ALPINIA GALANGA
METHANOL RHIZOMES EXTRACT
ABSTRACT
Objective: To investigate the inhibitory effect of methanol extract of Alpinia galanga rhizomes on the growth of
struvite crystals.
Methods: Struvite crystals were grown by the single diffusion gel growth technique and the inhibitory effect of
methanol extracts of Alpinia galanga rhizomes on the growth of struvite crystals has been studied. The grown
crystals were characterized by Fourier Transform Infrared Spectroscopy (FTIR) methods for further confirmations.
Results: With an increase in the concentration of methanol extract of Alpinia galanga rhizomes, the weight of the
formed crystals were gradually reduced from 2.66 g to 0.25 g in struvite crystals, respectively. The crystals
harvested from the struvite were characterized by Fourier Transform Infrared Spectroscopy (FTIR) to confirm the
functional groups. Results obtained indicated that Alpinia galanga rhizomes has the potential to inhibit the
formation of struvite crystals.
Conclusion: This study confirms that using methanol extract of Alpinia galanga rhizomes can promote the
formation of ammonium magnesium phosphate hexahydrate crystals and reduce the nucleation rate of struvite
crystals, a major component of urinary stone.
Key words: Struvite, Alpinia galanga rhizomes, Fourier Transform Infrared Spectroscopy (FTIR).
3. INTRODUCTION
Urolithiasis have been found in the tombs of Egyptian mummies dating back to 4000BC
Urolithiasis is a global problem and it is also called as nephrolithiasis or kidney stones
Urolithiasis is the presence of uroliths or calculi in the urinary tract
Genetic predisposition, metabolic disorders and poor intake of water are the causes of kidney stones
It is commonly found in males than females due to a longer urethra
Kidney stone is also called renal calculi
Urolithiasis and nephrolithiasis refers to the condition of having calculi in the urinary tract and
kidneys respectively
Crystals also form if the urine is too alkaline, too acidic or too concentrated.
Calcium stones – The common variety is Calcium Oxalate Monohydrate [ COM ]
TYPES OF KIDNEY STONES
Struvite stones – contains Magnesium
Uric acid stones – forms when there is too much acid in the urine
Cystine stones – occurrence is very rare
4. PATHOPHYSIOLOGY OF URINARY
STONE
Alkaline urine
The presence of urea or ammonia in the urine
High concentration of minerals in the urine
Kidney stones contain calcium oxalate, phosphate, magnesium and uric acid and
the formation of kidney stone includes :
Nucleation
Growth
Aggregation of kidney stone
5. REVIEW OF LITERATURE
The treatment and removal of renal calculi is often dependent upon the composition
and hardness of the stone , as well as stone size and placement in the urinary tract .
( Otnes , 1983; Dietrich et al.,1990)
In addition to the surgical management of the problem there are certain drugs and
other conventional therapies which are recommended for its control. ( Pearl et
al.,1990)
Lemon juice and orange juice are used as natural substances to inhibit calcium
oxalate crystallization under invitro ( Kulaksizoglu et al.,2007)
The aqueous extracts of Commiphora wightii dissolved struvite type of calculi to some
extent in an invitro model ( Chauhan et al., 2008)
6. OBJECTIVE
The growth characteristics of the most common types of urinary stones like struvite
crystals and the effect of methanol extracts of rhizomes of Alpinia galanga has been
studied
The identification of one Penta cyclic triterpenoid botulin from the rhizomes of Alpinia
galanga extract by HPTLC techniques
KINGDOM – PLANTAE
CLASS – ANGIOSPERMS
ORDER – ZINGIBERALES
FAMILY – ZINGIBERACEAE
GENUS – ALPINIA
SPECIES – GALANGA
DESCRIPTION - The plant grows from the rhizomes in clumps of stiff stalks upto 2m in
height with long leaves and red fruit
It is native to SOUTH ASIA and INDONESIA
The red fruit has a smell similar to cardamom
7. MATERIALS AND METHODS
Anhydrous methanol
Ammonium dihydrogen ortho phosphate
Magnesium acetate
Sodium metasilicate
Orthophosphoric acid
COLLECTION OF PLANT MATERIAL
The rhizome of Alpinia galanga were collected in the month of June, 2017 from Kolli
hills, Trichy, Tamil Nadu, India
The plant was identified and confirmed by Dr.S.John Britto , Director, Rapinat
Herbarium, St. Joseph’s College, Trichy
8. PREPARATION OF METHANOL
EXTRACTS
The rhizomes of Alpinia galanga were washed in running water, cut into small pieces
and then shade dried for a week at 35-40ºC.
After that it was grinded to a uniform powder of 40 mesh size (Doughari et al., 2007).
The methanol extracts were prepared by soaking 100 g of the dried powder plant
materials in 1 L of methanol by using a soxhlet extractor for 10 hours continuously.
The extracts were filtered through Whatmann filter paper No. 42 (125mm).
The filtered extract was concentrated and dried by using a rotary evaporator under
reduced pressure.
The obtained residue 1.2 g (rhizomes) was used to prepare the series of (0.15, 0.25,
0.50, 0.75 and 1.0%) methanol supernatant concentrations for in vitro studies .
9. PHYTOCHEMICAL SCREENING
Test for terpenoids – Salkowski test
Test for flavonoids
Foam test – saponin
Braymer’s test – tannin
Hager’s test – alkaloids
Salkowski test – steroids
Liebermann’s test – glycosides
Precipitate test – phlobatannins
Xantoproteic test – proteins
Test for coumarin , emodin, antroquinone, anthocyanin and carbohydrates
Keller Killiani test – cardiac glycosides
Test for phenol , leucoanthocyanin and xanthoprotein
10. IDENTIFICATION OF ACTIVE
COMPOUNDS FROM RHIZOMES BY
HPTLC
Sample preparation
Thin layer chromatography
Detection and estimation of betulin
Isolation of active compounds by column chromatography
Growth and characterization of struvite crystals
Statistical analysis
11. RESULTS AND DISCUSSIONS
CRYSTALS GROUP TREATENTS Harvested
crystals
(gram)
PERCENTAGE
OF INHIBITION
Struvite A Control 2.86 0%
B Control+Distilled water 2.32 18.8%
C Control+0.15% extracts 1.87 34.6%
D Control+0.25% extracts 1.72 39.8%
E Control+0.50% extracts 1.63 43.0%
F Control+0.75% extracts 0.52 81.8%
G
H
I
J
Control+1.00% extracts
Control+ fraction 7
Control+ fraction 8
Control+ fraction 12
0.43
0.72
0.83
0.64
84.9%
74.8%
70.9%
77.6%
S. No Phytochemical
constituents
Methanol extracts
of rhizomes of Alpinia
galanga
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
Tannin
Flavonoid
Terpenoid
Saponin
Phlobatanin
Steroid
Carbohydrate
Glycoside
Coumarin
Alkaloid
Protein
Emodin
Anthoquinone
Anthocyanine
Cardiac glycosides
Leucoanthocyanin
Phenol
Xanthoprotein
+
+
+
+
+
+
+
+
+
+
_
+
+
-
+
+
+
+
12.
13. SUMMARY AND CONCLUSION
Kidney stone disease is a common urinary stone disorder in humans and often cause severe pain,
which may lead to emergent hospitalization, shock wave lithotripsy and surgery.
Calcium oxalate is a major component of kidney stone. It forms upon the supersaturation of the urine
with calcium and other salts especially oxalate. The size of the stone can increase and obstruct in the
urinary system. Current pharmacological treatment of kidney stone consists of Gallium nitrate.
Although the most effective treatment of kidney stone is extracorporeal shock wave lithotripsy, the
side effects of this method are grave and can lead to recurrence of kidney stones. Therefore alternative
treatments are of high interest by using medicinal plants or phytotherapy.
With an increase in the concentration of methanol extract of rhizomes of Alpinia galanga ,the weight
of the formed crystals were gradually reduced from 2.43 g to 0.08 g in struvite crystals.
One way ANOVA performed with treated and untreated crystal growth data obtained from struvite
crystals showed significant differences (p<0.05)
The purpose of this dissertation is to investigate the beneficial effects of rhizomes of Alpinia galanga at
a different dose and single compounds for the prevention of kidney stone formation.
14. REFERENCES
Aggarwal, S. Tandon, C.D. Forouzanveh, M. Simgla, S.K. Kiran, R. and Jethi, R.K. 2000. Role of
biomolecules from human renal stone matrix on COM crystal growth. Molecular and cellular
Biochemistry., Vol.210, pp.109 –119.
Anantha Krishna Chaitanya, D. Santosh kumar, M. Monohar Reddy, A. Mukherjee, N.S.V. Sumanth,
N.H. and Ramesh, R. 2010. Antiurolithiatic activity of Macrotyloma uniflorum seed extract of ethylene
glycol induced urolithiasis in albino rats, Vol.1(5), pp.216-226.
Anbu, J. Suman, S. Swaroop Kumar, K. Satheesh Kumar, R. Nithya, S, Kannadhasan. 2011.
Antiurolithiatic activity of ethyl acetate root extract of Ichnocarpus frutescene using ethylene glycol
induced method in rats. J. Pharma. Sci. and Research., Vol. 3(4), pp. 1182-1189.
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