The document provides an overview of the DISTRICT+ project which aimed to promote knowledge transfer and innovation between research institutions and businesses in European regions. It summarizes 6 sub-projects that were part of DISTRICT+: EAST INNO Transfer, KNOW-ECO, NGSP, NICER, SPWW, and STEP. Each sub-project identified and shared good practices from their work. In total 43 good practices are summarized related to topics like science parks, clusters, funding for innovation, and knowledge transfer partnerships. The document concludes by discussing future opportunities for regional innovation under Horizon 2020 and smart specialization strategies.