DNA in Genetic Engineering
• • DNA is the genetic blueprint of life.
• • In genetic engineering, DNA is modified or
inserted into organisms.
• Example: Inserting human insulin gene into
bacteria to produce insulin.
Restriction Enzymes
• • Also known as molecular scissors.
• • Cut DNA at specific sequences.
• Example: EcoRI cuts DNA at GAATTC.
• • Used to isolate or remove specific genes.
DNA Ligases
• • Act like molecular glue.
• • Join DNA fragments together by forming
bonds.
• Example: Ligase joins a human gene into a
bacterial plasmid.
• • Essential for forming recombinant DNA.
Transgenic Organisms
• • Organisms with genes from another species.
• Example: Bt corn has a gene from a bacterium
to resist pests.
• • Used in agriculture, research, and medicine.
Gene Therapy
• • Technique to treat or cure genetic disorders.
• • Healthy genes are inserted into patient cells.
• Example: Using a virus vector to deliver a
functional gene in cystic fibrosis patients.
• • Still under research but has promising
results.
Conclusion
• • Genetic engineering involves manipulating
DNA using various tools.
• • Restriction enzymes and ligases are essential
for gene editing.
• • Applications include transgenic organisms
and gene therapy for disease treatment.

Genetic_Engineering_application and history

  • 1.
    DNA in GeneticEngineering • • DNA is the genetic blueprint of life. • • In genetic engineering, DNA is modified or inserted into organisms. • Example: Inserting human insulin gene into bacteria to produce insulin.
  • 2.
    Restriction Enzymes • •Also known as molecular scissors. • • Cut DNA at specific sequences. • Example: EcoRI cuts DNA at GAATTC. • • Used to isolate or remove specific genes.
  • 3.
    DNA Ligases • •Act like molecular glue. • • Join DNA fragments together by forming bonds. • Example: Ligase joins a human gene into a bacterial plasmid. • • Essential for forming recombinant DNA.
  • 4.
    Transgenic Organisms • •Organisms with genes from another species. • Example: Bt corn has a gene from a bacterium to resist pests. • • Used in agriculture, research, and medicine.
  • 5.
    Gene Therapy • •Technique to treat or cure genetic disorders. • • Healthy genes are inserted into patient cells. • Example: Using a virus vector to deliver a functional gene in cystic fibrosis patients. • • Still under research but has promising results.
  • 6.
    Conclusion • • Geneticengineering involves manipulating DNA using various tools. • • Restriction enzymes and ligases are essential for gene editing. • • Applications include transgenic organisms and gene therapy for disease treatment.