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Gel Electrophoresis
Gel electrophoresis is a way of visualizing how DNA has been cut up by separating DNA fragments according to their size.
The process uses some basic properties of DNA and of the gel setup.
Match the five bullet pointed statements with a reason from the second column and an outcome from the third column.
Rewrite the sentences

                                           because they can conduct electricity            so DNA will move on the
      The porous agarose gel is
                                                                                           agarose gel
      used.

                                           Because they move through the pores             so DNA will move on the
      Buffer solutions containing
                                           less easily                                     agarose gel
      ions are used.

                                           because they move through the pores             so they travel the shortest
      DNA is negatively charged
                                           more easily.                                    distance.

                                           because it allows the DNA to move               so DNA will move towards a
      Smaller fragments move
                                           through the pores.                              positive electrode
      faster

                                           because of the phosphate groups in the          so they travel the furthest
      Larger fragments move more
                                           backbone
      slowly

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Gel Electrophoresis

  • 1. Gel Electrophoresis Gel electrophoresis is a way of visualizing how DNA has been cut up by separating DNA fragments according to their size. The process uses some basic properties of DNA and of the gel setup. Match the five bullet pointed statements with a reason from the second column and an outcome from the third column. Rewrite the sentences because they can conduct electricity so DNA will move on the The porous agarose gel is agarose gel used. Because they move through the pores so DNA will move on the Buffer solutions containing less easily agarose gel ions are used. because they move through the pores so they travel the shortest DNA is negatively charged more easily. distance. because it allows the DNA to move so DNA will move towards a Smaller fragments move through the pores. positive electrode faster because of the phosphate groups in the so they travel the furthest Larger fragments move more backbone slowly