After Prussia's victory over Austria in 1866: 1) Napoleon III of France became deeply concerned by Prussia's growing power and sought to limit Prussian expansion. 2) Tensions rose further during the Luxembourg Crisis of 1866, when Bismarck opposed French influence in the region, damaging Franco-Prussian relations. 3) Over the following years disputes over succession to the Spanish throne and interpretations of communications like the Ems Telegram increased tensions until the Franco-Prussian War broke out in 1870, culminating in Prussia's victory and the unification of Germany under Prussian leadership.