Fluorescent virus like particles design
•Download as PPT, PDF•
1 like•45 views
Fluorescent virus-like particles design represent a convenient tool for characterization of virus-specific mechanisms and visualizing receptor-ligand interactions. https://www.creative-biolabs.com/vaccine/fluorescent-virus-like-particles-design.htm
Report
Share
Report
Share
Recommended
Detection of plant Pathogens
This document discusses advance portable tools for on-site detection of plant pathogens. It covers various disease detection tools including visual observation, cultural techniques, and the Foldscope microscope. It also discusses indirect detection methods like thermography, fluorescence imaging, hyperspectral techniques, and gas chromatography. Biosensor approaches for plant pathogen detection are also presented, including antibody-based biosensors, optical immunosensors, fluorescent approaches using quantum dots, and surface plasmon resonance systems. Loop mediated isothermal amplification is also discussed as a rapid detection method. The document emphasizes the importance of early detection in plant disease management and hopes that more portable detection devices will be developed to support efficient on-site diagnosis.
Experimental evidence to Prove RNA as genetic material
1. Experiments conducted by Fraenkel-Conrat and Singer on tobacco mosaic virus (TMV) provided evidence that RNA can act as genetic material.
2. They were able to separate the TMV into RNA and protein components, and found that RNA alone was able to cause infection when introduced into tobacco plants, demonstrating that RNA carries the genetic information.
3. They also generated chimeric viruses by combining the RNA of one TMV strain with the proteins of another strain. These chimeras displayed characteristics dependent on the source of the RNA, not the proteins, proving that the specificity of viral proteins is determined by the RNA.
Current methods for plant disease diagnosis
Shivani presents an overview of current and prospective methods for plant disease detection. Several techniques are discussed including serological based methods like ELISA and lateral flow devices, nucleic acid based methods like PCR and real-time PCR, optical sensor based methods like fluorescence imaging and hyperspectral imaging, and digital imaging based methods. Examples of each technique being used to detect various plant viruses, bacteria, fungi, and nematodes are provided. The techniques allow for rapid, sensitive, and accurate detection of pathogens before symptom development.
Virology techniques
this presentation gives an ideas about the virology techniques used for the identification of virus in brief
Scientific Sessions 2015: Typing of STI pathogens
Scientific Sessions 2015: Typing of STI pathogensSri Lanka College of Sexual Health and HIV Medicine
1) Typing, or characterizing, pathogens involves differentiating strains based on phenotypic or genotypic differences to aid epidemiological investigations. It is important for recognizing outbreaks and transmission patterns.
2) There are phenotypic methods like serotyping, biotyping, and phage typing, as well as genotypic methods like PCR, pulsed-field gel electrophoresis, and whole genome sequencing. The ideal typing system is reproducible, discriminatory, easy to interpret, and applicable to many pathogens.
3) Important pathogens that can be typed include HIV-1, Hepatitis B and C viruses, human papillomavirus, Chlamydia trachomatis, and Neisseria gonorrhoeaeOligonucleotide ligation assay presentation
PCR-OLA is a technique that combines polymerase chain reaction with oligonucleotide ligation assay to detect single nucleotide polymorphisms by distinguishing between ligation and non-ligation of oligonucleotides. It involves two phases - a multiplex PCR amplification to amplify the target DNA, followed by a multiplex oligonucleotide ligation assay where detection probes hybridize adjacent to each other and are ligated if complementary, which can identify normal vs. mutant genotypes. Ligation is regulated by the specificity of oligonucleotide hybridization, the need for adjacent hybridization of probes, and perfect complementarity of two bases.
Satellite RNA
in this chapter covers the symptoms modulation and diseases severity increases and decreases. and role of SiRNA in diseases severity reduction. and also covers the types of SRNA..
Sat rna1
This document provides an overview of satellite viruses and satellite RNAs (satRNAs). It defines them as subviral agents that depend on "helper viruses" for replication, movement, and transmission. Satellites are classified into different categories, including satellite viruses, large and small single-stranded satRNAs, and circular satRNAs. Recent research has provided insights into how satellites interact with their helper viruses and modulate symptoms. Satellites also have potential applications as vectors for gene expression or silencing in plants.
Recommended
Detection of plant Pathogens
This document discusses advance portable tools for on-site detection of plant pathogens. It covers various disease detection tools including visual observation, cultural techniques, and the Foldscope microscope. It also discusses indirect detection methods like thermography, fluorescence imaging, hyperspectral techniques, and gas chromatography. Biosensor approaches for plant pathogen detection are also presented, including antibody-based biosensors, optical immunosensors, fluorescent approaches using quantum dots, and surface plasmon resonance systems. Loop mediated isothermal amplification is also discussed as a rapid detection method. The document emphasizes the importance of early detection in plant disease management and hopes that more portable detection devices will be developed to support efficient on-site diagnosis.
Experimental evidence to Prove RNA as genetic material
1. Experiments conducted by Fraenkel-Conrat and Singer on tobacco mosaic virus (TMV) provided evidence that RNA can act as genetic material.
2. They were able to separate the TMV into RNA and protein components, and found that RNA alone was able to cause infection when introduced into tobacco plants, demonstrating that RNA carries the genetic information.
3. They also generated chimeric viruses by combining the RNA of one TMV strain with the proteins of another strain. These chimeras displayed characteristics dependent on the source of the RNA, not the proteins, proving that the specificity of viral proteins is determined by the RNA.
Current methods for plant disease diagnosis
Shivani presents an overview of current and prospective methods for plant disease detection. Several techniques are discussed including serological based methods like ELISA and lateral flow devices, nucleic acid based methods like PCR and real-time PCR, optical sensor based methods like fluorescence imaging and hyperspectral imaging, and digital imaging based methods. Examples of each technique being used to detect various plant viruses, bacteria, fungi, and nematodes are provided. The techniques allow for rapid, sensitive, and accurate detection of pathogens before symptom development.
Virology techniques
this presentation gives an ideas about the virology techniques used for the identification of virus in brief
Scientific Sessions 2015: Typing of STI pathogens
Scientific Sessions 2015: Typing of STI pathogensSri Lanka College of Sexual Health and HIV Medicine
1) Typing, or characterizing, pathogens involves differentiating strains based on phenotypic or genotypic differences to aid epidemiological investigations. It is important for recognizing outbreaks and transmission patterns.
2) There are phenotypic methods like serotyping, biotyping, and phage typing, as well as genotypic methods like PCR, pulsed-field gel electrophoresis, and whole genome sequencing. The ideal typing system is reproducible, discriminatory, easy to interpret, and applicable to many pathogens.
3) Important pathogens that can be typed include HIV-1, Hepatitis B and C viruses, human papillomavirus, Chlamydia trachomatis, and Neisseria gonorrhoeaeOligonucleotide ligation assay presentation
PCR-OLA is a technique that combines polymerase chain reaction with oligonucleotide ligation assay to detect single nucleotide polymorphisms by distinguishing between ligation and non-ligation of oligonucleotides. It involves two phases - a multiplex PCR amplification to amplify the target DNA, followed by a multiplex oligonucleotide ligation assay where detection probes hybridize adjacent to each other and are ligated if complementary, which can identify normal vs. mutant genotypes. Ligation is regulated by the specificity of oligonucleotide hybridization, the need for adjacent hybridization of probes, and perfect complementarity of two bases.
Satellite RNA
in this chapter covers the symptoms modulation and diseases severity increases and decreases. and role of SiRNA in diseases severity reduction. and also covers the types of SRNA..
Sat rna1
This document provides an overview of satellite viruses and satellite RNAs (satRNAs). It defines them as subviral agents that depend on "helper viruses" for replication, movement, and transmission. Satellites are classified into different categories, including satellite viruses, large and small single-stranded satRNAs, and circular satRNAs. Recent research has provided insights into how satellites interact with their helper viruses and modulate symptoms. Satellites also have potential applications as vectors for gene expression or silencing in plants.
Coronavirus and NMD (nonsense mediated mRNA decay)
This presentation discusses the interplay between coronaviruses and the host cell's nonsense-mediated mRNA decay (NMD) pathway. The presentation shows that:
1) Murine hepatitis virus (MHV) genomic and sub-genomic RNAs are targeted by the host cell's NMD machinery early in infection.
2) Depletion of NMD factors in host cells leads to increased levels of MHV proteins and higher virus titers, indicating NMD targets viral RNAs.
3) The MHV N protein helps inhibit the NMD pathway and makes viral mRNAs resistant to NMD, allowing for increased viral protein synthesis and replication.
Recombinant DNA, probes and Techniques
Recombinant DNA involves creating DNA molecules from different sources. Researchers improved fluorescent probes used to detect reactive oxygen species by replacing hydrogen with deuterium in the dyes. This increased the dyes' stability, shelf life, and ability to detect smaller concentrations of reactive oxygen species. Fluoromodules are fluorescent probes that monitor biological activities of individual proteins in real time. Researchers enhanced fluoromodule technology, creating probes that glow brighter than typical fluorescent proteins and allow proteins to be followed in real time. Fluorescent probes are essential for studying diseases like cancer and atherosclerosis as they detect and measure reactive oxygen species, which play a role in disease processes.
RNA as a genetic material
RNA structure is similar to DNA but contains the sugar ribose instead of deoxyribose and the base uracil instead of thymine. RNA can form secondary structures through internal base pairing. Some viruses use RNA as their genetic material, including tobacco mosaic virus (TMV) which contains only RNA and protein. Experiments in the 1950s-60s showed that the RNA of TMV determines the properties of progeny viruses and carries the genetic information rather than the protein, proving that RNA acts as the genetic material for some viruses.
Nano Flow Cytometer by NanoFCM Inc.
For many years scientists yearned for the possibility of performing flow cytometry to analyse small bio-nanoparticles that are too small to be measured by conventional and high sensitivity instruments. These entities, extracellular vesicles, gene therapy vectors, viruses and drug delivery particles, are promised to become the next generation of therapeutics, but they have been hard to handle and characterise due to their small size and biological or chemical heterogeneity. There is therefore a strong case for bringing flow cytometry capability to the sub-200nm scale.
NanoFCM has developed the NanoAnalyzer platform that now enables true flow-cytometry measurement at the sub-micron scale, and down to particle sizes unreachable by any other flow cytometers (10-40nm depending on the nature of the substrate). Nano-flow cytometry, the technology that underpins the NanoAnalyzer, removes bias and uncertainty stemming from the use of fluorescence signal triggering by using its highly sensitive side-scatter channel to trigger particle events. The single-particle nature of the measurement prevents uncontrolled swarming events, reinforcing data integrity. High resolution of both scatter and fluorescence channels allows the assessment of subpopulations, based on size or on bio-chemical properties.
Nano-flow cytometry’s ability to measure simultaneously a (bio)-nanoparticle population for size, size distribution and bio-chemical properties on a single instrument dramatically improves data quality and throughput compared to the traditional, multiple-techniques approach involving particle characterisation and counting (DLS, NTA, RPS), combined with chemical and biological function assessment (ELISA, Western Blot, Flow Cytometry, PCR). Quantitative measurement of the active and contaminant particles in a single preparation opens up the possibility of characterisation-based nanomedicine regulatory approval, and allows the conduct of large-scale clinical studies. From the research laboratory to the quality control department, NanoFCM delivers comprehensive bio-nano analysis.
Satellite RNA
This document discusses satellite RNA, which are small non-coding RNAs that depend on helper viruses for replication and encapsidation. It provides 3 key points:
1) Satellite RNAs alter symptoms of their helper viruses. They do not encode their own replication machinery and instead rely on the helper virus and plant cells. This makes them useful for studying helper virus replication.
2) Satellite RNAs can accumulate to high levels and be developed into expression vectors. They compete with helper virus RNA for replication, which can reduce accumulation of the helper virus.
3) Satellite RNAs can modulate or exacerbate disease symptoms depending on their sequence and interaction with the helper virus strain and host plant. They may attenuate symptoms
Pankaj omvs biochem symposium
Neisserial outer membrane vesicles (OMVs) are potent carriers of virulence factors. PorB is a channel-forming protein found in large quantities in the outer membrane of Neisseria species that plays a role in pathogenesis. This study investigated whether OMVs are a major secretion system for PorB and the effects of PorB and OMVs on host cells. The results showed that PorB is highly abundant in and on OMVs isolated from Neisseria gonorrhoeae. Additionally, OMVs were found to have toxic effects on host cells and PorB carried by OMVs was found to co-localize with the mitochondrial outer membrane.
Phage typing
Phage typing is a phenotypic method used to differentiate between bacterial strains using bacteriophages (viruses that infect bacteria). Certain bacteriophages can only infect specific bacterial strains. Phage typing involves growing bacterial cultures and spotting them with different bacteriophages - susceptible strains will show clearings where the bacteria have been lysed. While a complex method, phage typing has been useful for epidemiological surveillance and tracing outbreaks of pathogens like Salmonella typhimurium and Staphylococcus aureus. Improved standardization is needed to ensure reliable comparison of results between laboratories.
Ppt for seminar
This document summarizes detection methods for tospoviruses. It discusses several methods including symptomology, transmission studies, physical properties analysis, electron microscopy, serological techniques like ELISA and dot blot, nucleic acid-based methods like hybridization and PCR. ELISA and PCR methods are widely used now for accurate diagnosis due to their sensitivity, specificity and ability to detect viruses in mixed infections. Electron microscopy can be used to observe viral particles while transmission studies provide information on host range. Together these methods help identify and characterize tospoviruses.
Modern Techniques for Detection of Seed Born Fungi.
This presentation deals with modern (Serological, Molecular and other latest) detection techniques for seed borne fungal pathogens.
molecular detection of tuberculosis and rifampin resistance.
This document summarizes a study evaluating a rapid molecular test called Xpert MTB/RIF for detecting Mycobacterium tuberculosis (MTB) and resistance to rifampin directly from sputum samples. Three sputum specimens from patients were tested with the Xpert MTB/RIF assay, which can detect MTB and rifampin resistance from untreated sputum in under 2 hours. The results showed the Xpert MTB/RIF test provided sensitive detection of tuberculosis and rifampin resistance directly from sputum with minimal hands-on time.
Plant disease diagnosis
This document discusses methods for identifying plant pathogens. Traditional visual examination can only identify damage after it has already occurred. More sensitive early diagnosis methods are needed to treat pathogens before irreparable damage. Modern methods like polymerase chain reaction (PCR) and serological techniques can identify pathogens before visible symptoms appear, allowing treatment before significant yield losses. These methods help identify the causal agent through DNA analysis and other laboratory techniques.
Pcr single strand conformation polymorphism
This document discusses PCR-SSCP (single-strand conformation polymorphism) analysis, which is a sensitive technique for detecting mutations. It works by separating double-stranded PCR products into single strands, which take on conformations depending on their sequence. Minor mutations can alter the conformation and cause bands to shift on a gel. The technique is useful for detecting unknown single nucleotide polymorphisms and has various applications in clinical diagnosis, forensics, and studying genetics in animals, plants, and microbes.
Selection of tomato plants resistant to a local TSWV strain
This document summarizes research on selecting tomato plants resistant to Tomato Spotted Wilt Virus (TSWV) in Poland. Molecular marker and greenhouse testing identified lines resistant to a local TSWV isolate. The resistance gene Sw-5 was found to confer resistance and PCR with the SCAR 421 marker correctly identified plants as homozygous resistant, heterozygous, or homozygous susceptible. Greenhouse exposure to thrips and the virus confirmed resistant plants showed no disease symptoms while susceptible plants were infected. ELISA validated the presence or absence of the virus. The research provides new breeding lines with TSWV resistance for the Polish agricultural industry.
SEROLOGICAL METHODS FOR DETECTION OF PLANT PATHOGENS
This document discusses serological methods for detecting plant pathogens. It explains that serodiagnosis involves inducing an immune response in an animal to produce antibodies against a pathogen's antigens. These antibodies can then be used to detect the presence of the pathogen. The document describes several serological testing methods including ring interface tests, microprecipitin tests, double diffusion tests, ELISA, immunosorbent electron microscopy, and immunofluorescent staining. It concludes that serodiagnosis is a sensitive tool for identifying pathogens, detecting infections, and quantifying crop diseases.
Ppt
This document discusses using a lensfree holographic microscope to detect herpes simplex virus 1 (HSV-1). The microscope aims to provide a cost-effective method for viral counting that does not require fluorophore labeling. Specificity tests show the microscope can distinguish between antibody-conjugated HSV-1 and unlabeled virus. The limit of detection is estimated at 4 viral particles per mm2 field of view, allowing detection of around 120 counts within a typical field of view. However, low recovery rates could limit the technique due to factors like conjugation efficiency and viral fragmentation. Overall, the lensfree microscope provides a light, mobile and affordable option for sizing particles from 40nm to mm and detecting viral densities.
Detection of banana viruses in weed plants through dac elisa
it contain the detail of banana viruses in weed plants using DAC-ELISA. This is more use full for your study and project work
The Role of Epstein Barr Virsus in Oncogenesis
This document summarizes previous research on the Epstein-Barr virus (EBV) and describes a study that mapped interactions between EBV proteins and human proteins. EBV infects 95% of humans and can cause cancers. The study used a yeast two-hybrid method to screen 216 EBV proteins against 15,483 human proteins, identifying 188 interacting pairs. Mapping these virus-host interactions may reveal how EBV disrupts cellular pathways to cause disease.
Bhadana seminar (2)
This document discusses marker assisted selection in crop improvement. It provides an overview of the history and development of genetic markers. Different types of markers are described, including morphological, biochemical, and DNA-based molecular markers. The document discusses various molecular marker techniques such as RFLP, RAPD, AFLP, and their characteristics and applications in genetic mapping, gene tagging, and marker-assisted selection in plant breeding.
IBMM Proteomic Platform - ULB - Lab'InSight Proteomics
The document summarizes the capabilities and services of the IBMM Proteomic Platform located at the Université Libre de Bruxelles. The platform offers protein separation and identification services using 2D gel electrophoresis, mass spectrometry, and other techniques. It has facilities for gel-based and LC-MS/MS proteomics, post-translational modification analysis, and is currently working on several research projects involving bacterial genetics, parasitology, molecular embryology, and other areas. The platform aims to provide routine analysis of protein samples as well as develop new proteomics methods.
Gonzalez_Emilia_Poster3
This study investigated the non-lytic spread of poliovirus via exosomes. The researchers found that during poliovirus infection, double-membraned vesicles resembling autophagosomes form and contain viral particles. They hypothesized these vesicles could fuse with the cell membrane and release viruses in exosomes. When isolating exosomes from infected cell supernatants, they detected the exosomal proteins CD81 and LC3 as well as viral RNA, suggesting poliovirus may spread non-lytically in exosomes. Future work will aim to detect viral proteins in exosomes and optimize RNA detection methods.
reverse-line-blot-hybridisation-in-the-detection-of-tick-borne-diseases
The document describes the development of a reverse line blot (RLB) hybridization kit that can simultaneously detect four genera of common tick-borne pathogens - Anaplasma, Ehrlichia, Babesia and Theileria. This provides a sensitive and cost-effective diagnostic tool that facilitates the study of tick-borne diseases. The RLB technique involves PCR amplification of the pathogens, followed by hybridization of the products on a membrane containing genus-specific probes. This allows for simultaneous identification of any of the pathogens present in one sample. The kit will support improved diagnosis and epidemiological research on these important diseases affecting both animals and humans.
Immunofluoresce-WPS Office.pdf
Immunofluorescence is a technique allowing
the visualization of a specific antigen by
bindIng a specific antibody chemically
conjugated with a fluorescent dye.
More Related Content
What's hot
Coronavirus and NMD (nonsense mediated mRNA decay)
This presentation discusses the interplay between coronaviruses and the host cell's nonsense-mediated mRNA decay (NMD) pathway. The presentation shows that:
1) Murine hepatitis virus (MHV) genomic and sub-genomic RNAs are targeted by the host cell's NMD machinery early in infection.
2) Depletion of NMD factors in host cells leads to increased levels of MHV proteins and higher virus titers, indicating NMD targets viral RNAs.
3) The MHV N protein helps inhibit the NMD pathway and makes viral mRNAs resistant to NMD, allowing for increased viral protein synthesis and replication.
Recombinant DNA, probes and Techniques
Recombinant DNA involves creating DNA molecules from different sources. Researchers improved fluorescent probes used to detect reactive oxygen species by replacing hydrogen with deuterium in the dyes. This increased the dyes' stability, shelf life, and ability to detect smaller concentrations of reactive oxygen species. Fluoromodules are fluorescent probes that monitor biological activities of individual proteins in real time. Researchers enhanced fluoromodule technology, creating probes that glow brighter than typical fluorescent proteins and allow proteins to be followed in real time. Fluorescent probes are essential for studying diseases like cancer and atherosclerosis as they detect and measure reactive oxygen species, which play a role in disease processes.
RNA as a genetic material
RNA structure is similar to DNA but contains the sugar ribose instead of deoxyribose and the base uracil instead of thymine. RNA can form secondary structures through internal base pairing. Some viruses use RNA as their genetic material, including tobacco mosaic virus (TMV) which contains only RNA and protein. Experiments in the 1950s-60s showed that the RNA of TMV determines the properties of progeny viruses and carries the genetic information rather than the protein, proving that RNA acts as the genetic material for some viruses.
Nano Flow Cytometer by NanoFCM Inc.
For many years scientists yearned for the possibility of performing flow cytometry to analyse small bio-nanoparticles that are too small to be measured by conventional and high sensitivity instruments. These entities, extracellular vesicles, gene therapy vectors, viruses and drug delivery particles, are promised to become the next generation of therapeutics, but they have been hard to handle and characterise due to their small size and biological or chemical heterogeneity. There is therefore a strong case for bringing flow cytometry capability to the sub-200nm scale.
NanoFCM has developed the NanoAnalyzer platform that now enables true flow-cytometry measurement at the sub-micron scale, and down to particle sizes unreachable by any other flow cytometers (10-40nm depending on the nature of the substrate). Nano-flow cytometry, the technology that underpins the NanoAnalyzer, removes bias and uncertainty stemming from the use of fluorescence signal triggering by using its highly sensitive side-scatter channel to trigger particle events. The single-particle nature of the measurement prevents uncontrolled swarming events, reinforcing data integrity. High resolution of both scatter and fluorescence channels allows the assessment of subpopulations, based on size or on bio-chemical properties.
Nano-flow cytometry’s ability to measure simultaneously a (bio)-nanoparticle population for size, size distribution and bio-chemical properties on a single instrument dramatically improves data quality and throughput compared to the traditional, multiple-techniques approach involving particle characterisation and counting (DLS, NTA, RPS), combined with chemical and biological function assessment (ELISA, Western Blot, Flow Cytometry, PCR). Quantitative measurement of the active and contaminant particles in a single preparation opens up the possibility of characterisation-based nanomedicine regulatory approval, and allows the conduct of large-scale clinical studies. From the research laboratory to the quality control department, NanoFCM delivers comprehensive bio-nano analysis.
Satellite RNA
This document discusses satellite RNA, which are small non-coding RNAs that depend on helper viruses for replication and encapsidation. It provides 3 key points:
1) Satellite RNAs alter symptoms of their helper viruses. They do not encode their own replication machinery and instead rely on the helper virus and plant cells. This makes them useful for studying helper virus replication.
2) Satellite RNAs can accumulate to high levels and be developed into expression vectors. They compete with helper virus RNA for replication, which can reduce accumulation of the helper virus.
3) Satellite RNAs can modulate or exacerbate disease symptoms depending on their sequence and interaction with the helper virus strain and host plant. They may attenuate symptoms
Pankaj omvs biochem symposium
Neisserial outer membrane vesicles (OMVs) are potent carriers of virulence factors. PorB is a channel-forming protein found in large quantities in the outer membrane of Neisseria species that plays a role in pathogenesis. This study investigated whether OMVs are a major secretion system for PorB and the effects of PorB and OMVs on host cells. The results showed that PorB is highly abundant in and on OMVs isolated from Neisseria gonorrhoeae. Additionally, OMVs were found to have toxic effects on host cells and PorB carried by OMVs was found to co-localize with the mitochondrial outer membrane.
Phage typing
Phage typing is a phenotypic method used to differentiate between bacterial strains using bacteriophages (viruses that infect bacteria). Certain bacteriophages can only infect specific bacterial strains. Phage typing involves growing bacterial cultures and spotting them with different bacteriophages - susceptible strains will show clearings where the bacteria have been lysed. While a complex method, phage typing has been useful for epidemiological surveillance and tracing outbreaks of pathogens like Salmonella typhimurium and Staphylococcus aureus. Improved standardization is needed to ensure reliable comparison of results between laboratories.
Ppt for seminar
This document summarizes detection methods for tospoviruses. It discusses several methods including symptomology, transmission studies, physical properties analysis, electron microscopy, serological techniques like ELISA and dot blot, nucleic acid-based methods like hybridization and PCR. ELISA and PCR methods are widely used now for accurate diagnosis due to their sensitivity, specificity and ability to detect viruses in mixed infections. Electron microscopy can be used to observe viral particles while transmission studies provide information on host range. Together these methods help identify and characterize tospoviruses.
Modern Techniques for Detection of Seed Born Fungi.
This presentation deals with modern (Serological, Molecular and other latest) detection techniques for seed borne fungal pathogens.
molecular detection of tuberculosis and rifampin resistance.
This document summarizes a study evaluating a rapid molecular test called Xpert MTB/RIF for detecting Mycobacterium tuberculosis (MTB) and resistance to rifampin directly from sputum samples. Three sputum specimens from patients were tested with the Xpert MTB/RIF assay, which can detect MTB and rifampin resistance from untreated sputum in under 2 hours. The results showed the Xpert MTB/RIF test provided sensitive detection of tuberculosis and rifampin resistance directly from sputum with minimal hands-on time.
Plant disease diagnosis
This document discusses methods for identifying plant pathogens. Traditional visual examination can only identify damage after it has already occurred. More sensitive early diagnosis methods are needed to treat pathogens before irreparable damage. Modern methods like polymerase chain reaction (PCR) and serological techniques can identify pathogens before visible symptoms appear, allowing treatment before significant yield losses. These methods help identify the causal agent through DNA analysis and other laboratory techniques.
Pcr single strand conformation polymorphism
This document discusses PCR-SSCP (single-strand conformation polymorphism) analysis, which is a sensitive technique for detecting mutations. It works by separating double-stranded PCR products into single strands, which take on conformations depending on their sequence. Minor mutations can alter the conformation and cause bands to shift on a gel. The technique is useful for detecting unknown single nucleotide polymorphisms and has various applications in clinical diagnosis, forensics, and studying genetics in animals, plants, and microbes.
Selection of tomato plants resistant to a local TSWV strain
This document summarizes research on selecting tomato plants resistant to Tomato Spotted Wilt Virus (TSWV) in Poland. Molecular marker and greenhouse testing identified lines resistant to a local TSWV isolate. The resistance gene Sw-5 was found to confer resistance and PCR with the SCAR 421 marker correctly identified plants as homozygous resistant, heterozygous, or homozygous susceptible. Greenhouse exposure to thrips and the virus confirmed resistant plants showed no disease symptoms while susceptible plants were infected. ELISA validated the presence or absence of the virus. The research provides new breeding lines with TSWV resistance for the Polish agricultural industry.
SEROLOGICAL METHODS FOR DETECTION OF PLANT PATHOGENS
This document discusses serological methods for detecting plant pathogens. It explains that serodiagnosis involves inducing an immune response in an animal to produce antibodies against a pathogen's antigens. These antibodies can then be used to detect the presence of the pathogen. The document describes several serological testing methods including ring interface tests, microprecipitin tests, double diffusion tests, ELISA, immunosorbent electron microscopy, and immunofluorescent staining. It concludes that serodiagnosis is a sensitive tool for identifying pathogens, detecting infections, and quantifying crop diseases.
Ppt
This document discusses using a lensfree holographic microscope to detect herpes simplex virus 1 (HSV-1). The microscope aims to provide a cost-effective method for viral counting that does not require fluorophore labeling. Specificity tests show the microscope can distinguish between antibody-conjugated HSV-1 and unlabeled virus. The limit of detection is estimated at 4 viral particles per mm2 field of view, allowing detection of around 120 counts within a typical field of view. However, low recovery rates could limit the technique due to factors like conjugation efficiency and viral fragmentation. Overall, the lensfree microscope provides a light, mobile and affordable option for sizing particles from 40nm to mm and detecting viral densities.
Detection of banana viruses in weed plants through dac elisa
it contain the detail of banana viruses in weed plants using DAC-ELISA. This is more use full for your study and project work
The Role of Epstein Barr Virsus in Oncogenesis
This document summarizes previous research on the Epstein-Barr virus (EBV) and describes a study that mapped interactions between EBV proteins and human proteins. EBV infects 95% of humans and can cause cancers. The study used a yeast two-hybrid method to screen 216 EBV proteins against 15,483 human proteins, identifying 188 interacting pairs. Mapping these virus-host interactions may reveal how EBV disrupts cellular pathways to cause disease.
Bhadana seminar (2)
This document discusses marker assisted selection in crop improvement. It provides an overview of the history and development of genetic markers. Different types of markers are described, including morphological, biochemical, and DNA-based molecular markers. The document discusses various molecular marker techniques such as RFLP, RAPD, AFLP, and their characteristics and applications in genetic mapping, gene tagging, and marker-assisted selection in plant breeding.
IBMM Proteomic Platform - ULB - Lab'InSight Proteomics
The document summarizes the capabilities and services of the IBMM Proteomic Platform located at the Université Libre de Bruxelles. The platform offers protein separation and identification services using 2D gel electrophoresis, mass spectrometry, and other techniques. It has facilities for gel-based and LC-MS/MS proteomics, post-translational modification analysis, and is currently working on several research projects involving bacterial genetics, parasitology, molecular embryology, and other areas. The platform aims to provide routine analysis of protein samples as well as develop new proteomics methods.
Gonzalez_Emilia_Poster3
This study investigated the non-lytic spread of poliovirus via exosomes. The researchers found that during poliovirus infection, double-membraned vesicles resembling autophagosomes form and contain viral particles. They hypothesized these vesicles could fuse with the cell membrane and release viruses in exosomes. When isolating exosomes from infected cell supernatants, they detected the exosomal proteins CD81 and LC3 as well as viral RNA, suggesting poliovirus may spread non-lytically in exosomes. Future work will aim to detect viral proteins in exosomes and optimize RNA detection methods.
What's hot (20)
Coronavirus and NMD (nonsense mediated mRNA decay)
Coronavirus and NMD (nonsense mediated mRNA decay)
Modern Techniques for Detection of Seed Born Fungi.
Modern Techniques for Detection of Seed Born Fungi.
molecular detection of tuberculosis and rifampin resistance.
molecular detection of tuberculosis and rifampin resistance.
Selection of tomato plants resistant to a local TSWV strain
Selection of tomato plants resistant to a local TSWV strain
SEROLOGICAL METHODS FOR DETECTION OF PLANT PATHOGENS
SEROLOGICAL METHODS FOR DETECTION OF PLANT PATHOGENS
Detection of banana viruses in weed plants through dac elisa
Detection of banana viruses in weed plants through dac elisa
IBMM Proteomic Platform - ULB - Lab'InSight Proteomics
IBMM Proteomic Platform - ULB - Lab'InSight Proteomics
Similar to Fluorescent virus like particles design
reverse-line-blot-hybridisation-in-the-detection-of-tick-borne-diseases
The document describes the development of a reverse line blot (RLB) hybridization kit that can simultaneously detect four genera of common tick-borne pathogens - Anaplasma, Ehrlichia, Babesia and Theileria. This provides a sensitive and cost-effective diagnostic tool that facilitates the study of tick-borne diseases. The RLB technique involves PCR amplification of the pathogens, followed by hybridization of the products on a membrane containing genus-specific probes. This allows for simultaneous identification of any of the pathogens present in one sample. The kit will support improved diagnosis and epidemiological research on these important diseases affecting both animals and humans.
Immunofluoresce-WPS Office.pdf
Immunofluorescence is a technique allowing
the visualization of a specific antigen by
bindIng a specific antibody chemically
conjugated with a fluorescent dye.
Fluorophore Based Chemistries Used In Various Molecular Techniques
Fluorescent reporter molecules are used with various molecular technologies to monitor biological processes.
Nanomaterials for Virus Detection
1) Nanomaterials like gold nanoparticles, carbon nanotubes, and quantum dots show potential for virus detection through their unique optical and electrical properties.
2) Gold nanoparticle probes modified with influenza virus antibodies allow one-step, colorimetric detection of influenza without expensive equipment.
3) Carbon nanotube sensors could allow low-cost, routine monitoring for dengue virus detection by non-experts in places like clinics.
4) Quantum dot probes have been used to simultaneously track multiple viral proteins over time to study respiratory syncytial virus infection.
PCR & NON PCR BASED MARUTHI.pptx
This document discusses molecular techniques for the detection of plant viruses, including PCR and non-PCR based methods. It provides details on PCR including the basic principles, steps involved in PCR, advantages and disadvantages. It also discusses different types of PCR like multiplex PCR, nested PCR, reverse transcriptase PCR and real-time PCR. The document also discusses molecular hybridization techniques like nucleic acid hybridization, dot blot hybridization and fluorescence in situ hybridization for detection of plant pathogens.
Undergraduate Research Grant
This grant proposal aims to analyze protein-protein interactions within the type-III secretion system (T3SS) of the pathogenic bacterium Chromobacterium violaceum. The researchers will use molecular techniques like PCR and the yeast two-hybrid system to study interactions between 11 proteins that are thought to be involved in Cpi-2, one of two T3SSs found in C. violaceum. Understanding these interactions could help identify new drug targets and vaccine components to treat infections caused by this antibiotic-resistant bacterium. The proposal outlines experiments to clone the genes of interest, test protein interactions using yeast two-hybrid screens, and build an interaction map of the Cpi-2 T3SS apparatus.
Seminario biomol Maria Clara Torres Ferrer
The study aimed to identify if annexin A2 is a host target of the Salmonella pathogenicity island-2 effectors SopD2 and PipB2. The authors overexpressed the SsrB regulator to identify SPI-2 effectors during infection. Using techniques like immunofluorescence, SDS-PAGE, western blot, and mass spectrometry, they found that SopD2 targets annexin A2 in vitro. Their findings add to the understanding of how Salmonella effectors modify host cell processes during infection. The discussion reviews related literature, finding the authors generally agree that SPI-2 effectors modify the endosomal membrane to establish infection.
Immunofluorescence
Introduction, the principle of immunofluorescence, Technique, Fluorescent microscope and its components, Application and types of immunofluorescence, Direct and indirect immunofluorescence, FACS (Fluorescence-activated cell sorting), Uses and limitations of Immunofluorescence
The Biology of HIV-AIDS Acquired immune deficiency syndrome (AIDS) is.pdf
The Biology of HIV/AIDS Acquired immune deficiency syndrome (AIDS) is a disease
characterized by the progressive deterioration of an individual's immune system. The
immunological impairment allows infectious agents such as viruses, bacteria, fungi and parasites
to invade the body and propagate unchecked. In addition, the incidence of certain cancers
dramatically increases in these patients because of faulty immunosurveillance. AIDS is a serious
threat to human health and is a global problem. Intensive research is being done to advance
methods of detection, clinical treatment and prevention. The HIV Virus The AIDS etiologic
agent is the human immunodeficiency virus type 1 (HIV-1), a retrovirus. HIV-1 contains an
RNA genome and the RNA-dependent-DNA-polymerase termed reverse transcriptase. Members
of the retrovirus family are involved in the pathogenesis of certain types of leukemias and other
sarcomas in humans and animals. The structure and replication mechanism of HIV is very
similar to other retroviruses. However, HIV is unique in some of its properties - it specifically
targets the immune system, is very immunoevasive, forms significant amounts of progeny virus
in vivo during initial stages of infection and can be transmitted during sexual activity. The HIV
viral particle is surrounded by a lipid Human Immunodeficiency Virus bilayer derived from the
host cell membrane during budding. The viral proteins are identified by the prefix gp
(glycoprotein) or p (protein) followed by a number indicating the approximate molecular weight
in kilodaltons. The lipid bilayer contains gp120 and gp41. These two proteins are proteolytic
products of the precursor gp160. The gp41 anchors gp120 in the bilayer. The protein gp120 is
routinely used as a diagnostic marker for HIV in Western Blot Analysis. More recently other
viral gp proteins are also included in the test. Beneath the bilayer is a capsid consisting of p17
and p18. Within this shell is the viral core. The walls of the core consist of p 24 and p25. Within
the core are two identical RNA molecules, 9800 nucleotides in length. Hydrogen bonded to each
viral RNA is a cellular tRNA molecule. The viral RNA is coated by tightly bound molecules of p
7 and p 9 . The core also contains approximately 50 molecules of reverse transcriptase. There are
several other viral proteins whose precise functions are not fully understood. The virus can be
grown in tissue culture for diagnostic and research purposes. Several of the viral proteins have
been cloned and generated in relatively large quantities. An individual can receive an inoculum
of HIV through an abrasion in a mucosal surface (e.g., genital and rectal walls), a blood
transfusion, or by intravenous injection with a contaminated needle. Virus or virally infected
cells are found in body fluids such as semen and blood. The most important target for the virus is
hematopoietic cells such as bone marrow derived monocytes, myelocytes and lymphocytes.
Infection of im.
Dna microarray technique for detection and identification of VIRUS
DNA micro Array Technique is still under development which is very useful for identification and detection of highly dangerous viruses and pathogen.
Virus
Viruses consist of genetic material and proteins but lack cellular structure. They replicate by taking over host cell processes. There are two main classification systems - Baltimore system classifies based on mRNA synthesis, ICTV system uses genome properties and structure. Viruses can be isolated from samples and cultured in vivo or in vitro. They are quantified using plaque assays, PCR, ELISA or other methods. Bacteriophages follow lytic or lysogenic cycles to infect bacteria and can transfer genes between bacteria through transduction.
mego (M-1607) Master Seminar Presentation.pptx
Modern techniques for detection of plant pathogens
Megobi Punyü presented on modern techniques for detecting plant pathogens including serological, molecular, optical, and biosensor-based methods. The techniques aim to be specific, sensitive, accurate, reliable, fast, easy to use, cost-effective and able to detect pathogens in complex matrices. ELISA and PCR are commonly used serological and molecular methods. Optical techniques include fluorescence imaging, thermography, and hyperspectral imaging. Emerging biosensor methods utilize nanomaterials like carbon nanotubes and gold nanoparticles.
HIV/AIDS
A detailed description of HIV covering virology, morphology, pathogenesis, clinical stages and manifestations, laboratory diagnosis, and diagnostic strategy, and therapeutic options and prevention.
Molecular microbiology methods
1. Molecular microbiology methods like PCR and hybridization have revolutionized clinical diagnostics by enabling fast and direct detection of pathogens from clinical samples.
2. PCR in particular has become a mainstay technique, allowing amplification of specific DNA sequences from small amounts of input DNA. Variations like real-time PCR, multiplex PCR, and broad-range PCR further expanded diagnostic capabilities.
3. Emerging technologies like DNA microarrays promise even greater multiplexing, with the ability to simultaneously genotype large genomic regions or measure expression of many genes, positioning them as promising future molecular diagnostic tools.
Janse_2013_BMCID_Burkholderia
A single-reaction quadruplex qPCR assay was developed that can rapidly detect and differentiate Burkholderia mallei and Burkholderia pseudomallei. The assay uses three signature sequences - a multicopy transposase sequence common to both species for sensitive detection, and two unique sequences for species differentiation. It also incorporates an internal control for DNA extraction and amplification using Bacillus thuringiensis. The assay enables detection of less than 1 genome equivalent and differentiation of B. mallei and B. pseudomallei with high sensitivity and reliability for diagnostic and surveillance purposes.
Effects of chloroquine on viral infections
Chloroquine is a 9-aminoquinoline known since 1934. Apart from its well-known antimalarial effects, the drug has interesting biochemical properties that might be applied against some viral infections. Chloroquine exerts direct antiviral effects, inhibiting pH-dependent steps of the replication of several viruses including members of the flaviviruses, retroviruses, and coronaviruses. Its best-studied effects are those against HIV replication, which are being tested in clinical trials. Moreover, chloroquine has immunomodulatory effects, suppressing the production/release of tumour necrosis factor alpha and interleukin 6, which mediate the inflammatory complications of several viral diseases. We review the available information on the effects of chloroquine on viral infections, raising the question of whether this old drug may experience a revival in the clinical management of viral diseases such as AIDS and severe acute respiratory syndrome, which afflict mankind in the era of globalisation.
Microbiology lecture presentation-1.pptx
This document discusses microbial diagnostic tests and their applications. It begins by introducing the importance of microbial diagnostics in identifying pathogens that cause disease. It then describes various diagnostic test types including culture techniques, molecular diagnostics like PCR, immunodiagnostic tests, and biosensors. The document also discusses laboratory diagnosis of bacteria, fungi, and viruses through techniques like microscopy, culturing, serology, antigen detection, and nucleic acid detection. It emphasizes the role of diagnostic tests in facilitating appropriate treatment and antimicrobial stewardship.
Measuring parameters of Bovine Enterovirus infection
1) Four assays were performed to measure parameters of bovine enterovirus infection: plaque assay, TCID50 assay, viral protein assay, and intracellular viral RNA assay.
2) The plaque assay showed that virus titer increased with time as more replication cycles occurred.
3) The viral protein assay indicated variation in viral proteins produced at different time points of infection.
4) Unfortunately, the intracellular viral RNA assay did not detect any RNA from samples.
detectionofplantpathogensusingnon-pcrbasedtechniques-170112144243.pdf
This document discusses several non-PCR based molecular approaches for detecting and identifying plant pathogens, including:
1. Conventional techniques like visual observation of symptoms, culturing pathogens, and microscopy which have limitations.
2. Serological methods like monoclonal antibodies, antigen-antibody based techniques like ELISA, immunofluorescence, and hybridization based techniques.
3. Specific techniques discussed in detail include tube precipitation tests, microprecipitation tests, latex agglutination, gel diffusion tests, immuno-electrophoresis, DAS/DAC/DIBA ELISA, immuno-sorbent electron microscopy, lateral flow, dot immunoblotting, fluorescence microscopy, transmission electron microscopy, southern blotting, and flow cytometry
Development of diagnostic tools to reduce antimicrobial (mis)use: Novel ident...
Presentation by Elise Schieck and Sonal Henson at an ILRI Animal and Human Health program scientific seminar, 8 November 2021.
Similar to Fluorescent virus like particles design (20)
reverse-line-blot-hybridisation-in-the-detection-of-tick-borne-diseases
reverse-line-blot-hybridisation-in-the-detection-of-tick-borne-diseases
Fluorophore Based Chemistries Used In Various Molecular Techniques
Fluorophore Based Chemistries Used In Various Molecular Techniques
The Biology of HIV-AIDS Acquired immune deficiency syndrome (AIDS) is.pdf
The Biology of HIV-AIDS Acquired immune deficiency syndrome (AIDS) is.pdf
Dna microarray technique for detection and identification of VIRUS
Dna microarray technique for detection and identification of VIRUS
Measuring parameters of Bovine Enterovirus infection
Measuring parameters of Bovine Enterovirus infection
detectionofplantpathogensusingnon-pcrbasedtechniques-170112144243.pdf
detectionofplantpathogensusingnon-pcrbasedtechniques-170112144243.pdf
Development of diagnostic tools to reduce antimicrobial (mis)use: Novel ident...
Development of diagnostic tools to reduce antimicrobial (mis)use: Novel ident...
More from Creative Biolabs
What is oncolytic virus
Recently, the development of molecular biotechnology allows modifications of viral genomes genetically and optimizes the transformation of available viruses with weak pathogenicity. These methods are used to enhance the oncolytic effect and reduce adverse reactions to maximize both efficacy and safety. Indeed, the oncolytic virus can stimulate a pro-inflammatory tumor environment by enhancing antigen recognition and robust immune responses. It overcomes the immune evasiveness and escape of malignant cells to eliminate the tumor cells.
https://www.creative-biolabs.com/oncolytic-virus/definition-of-an-oncolytic-virus.htm
What is oncolytic virus therapy
An oncolytic virus is a form of promising therapeutic tool for the treatment of malignant tumors, which uses viruses to selectively infect and kill tumor cells and further to induce or boost specific antitumor immunity. https://www.creative-biolabs.com/oncolytic-virus/definition-of-an-oncolytic-virus.htm
Reporter-encoding Oncolytic Virus
Oncolytic viruses encoding reporter genes utilized for in vivo molecular imaging are useful to locate the distribution of oncolytic viruses in pre-clinical tests. Optical detection methods mainly include green fluorescent protein (GFP), enhanced GFP (eGFP), discosoma red fluorescent protein (DsRed), and bioluminescence imaging (BLI), which utilizes luciferases. Reporter-encoding oncolytic viruses, including vaccinia virus, adenovirus, herpes simplex virus and vesicular stomatitis virus, allow accurate tracking of gene expression, tumor metastases, viral infection as well as assessment of gene therapy.
https://www.creative-biolabs.com/oncolytic-virus/category-reporter-encoding-oncolytic-virus-293.htm
Pre-made Oncolytic VACV
Vaccinia virus can accommodate more than 30 kb of foreign DNA. Foreign genes can be stably integrated into the viral genome, resulting in efficient and long-term gene expression. The deletion of the viral genes of thymidine kinase (TK) and vaccinia growth factors (VGF) results in enhanced tumor-selectively and antitumor activity, and reduced virus virulence. https://www.creative-biolabs.com/oncolytic-virus/category-pre-made-oncolytic-vaccinia-virus-291.htm
Pre-made Oncolytic Vaccinia Virus
Oncolytic viruses are a class of antitumor agents that selectively kill tumor cells without affecting normal cells. Vaccinia virus (VACV) is a large, enveloped virus that is considered as the most potential live biotherapeutic agent because of its strong oncolytic efficacy and potent antigen presentation capability that can combine well with its natural oncolytic activities for cancer immunotherapy. Many types of modified vaccinia virus have been used for in vitro and in vivo studies, as well as clinical trials.https://www.creative-biolabs.com/oncolytic-virus/category-pre-made-oncolytic-vaccinia-virus-291.htm
Pre-made Oncolytic HSV
Partial deletion of the HSV gene results in superior packaging capacity of >30 kb foreign DNA with low toxicity as an expression vector. Multiple modified purified oncolytic herpes simplex virus (oHSV) products can avoid evading the host immune response and reduce toxicity by gene knock-out, such as ICP0, ICP4, ICP22, ICP27 or ICP47.https://www.creative-biolabs.com/oncolytic-virus/category-pre-made-oncolytic-herpes-simplex-virus-290.htm
Pre-made Oncolytic Herpes Simplex Virus
Oncolytic viruses are using for the treatment of cancer due to the specific antitumor activity in tumor cells. Herpes simplex virus (HSV) is a human neurogenic dsDNA virus that has the characteristic of life-long latent infection of neurons and allows for long-term transgene expression.https://www.creative-biolabs.com/oncolytic-virus/category-pre-made-oncolytic-herpes-simplex-virus-290.htm
Oncolytic Virus Delivery
Oncolytic viruses have the potential to powerfully and selectively kill cancer cells and have shown impressive efficacy in preclinical and clinical settings. However, their potential can be restricted by inefficient delivery into the complex tumor environment. Thus, the efficient delivery of oncolytic viruses remains a significant challenge in the field of oncology, limiting their therapeutic effect. https://www.creative-biolabs.com/oncolytic-virus/approaches-to-delivery-of-oncolytic-viruses.htm
Oncolytic Virus Application
Numerous viruses are being developed pre-clinically and clinically. An investigation of all registered clinical trials in 2017 demonstrates 78 interventional trials regarding OVs. This ability for near-universal therapeutic impact in cancer makes OVs a popular therapeutic tool. Today, both preclinical and early-stage clinical trials are intensively investigating the approach to improve oncolytic virotherapy.
https://www.creative-biolabs.com/oncolytic-virus/applications-of-oncolytic-viruses-in-cancer-treatment.htm
Oncolytic Virus Animal Model
To fully optimize oncolytic virotherapy and provide meaningful mechanistic insight, it is important to have representative animal models of oncolysis in various tumor types. https://www.creative-biolabs.com/oncolytic-virus/animal-models-for-oncolytic-virus-study.htm
Abciximab mechanism of action
Abciximab (also known as abcixifiban or c7E3 Fab), is the Fab fragment of the chimeric human-murine, monoclonal antibody 7E3. It is composed of murine variable regions and human constant regions.https://www.creativebiolabs.net/abciximab-overview.htm
Abagovomab mechanism of action
Abagovomab is a murine monoclonal anti-idiotypic antibody (MW: 165-175 kDa), produced by a mouse hybridoma and generated against OC125, which serves to functionally imitate the human cancer antigen 125 (CA-125). https://www.creativebiolabs.net/abagovomab-overview.htm
Wnt Pathway
Wnt comprises a diverse family of secreted lipid-modified signaling glycoproteins that are 350-400 amino acids in length. Wnt is an acronym in the field of genetics that stands for 'Wingless/Integrated'.https://www.creativebiolabs.net/wnt-signaling-pathway.htm
TNF Pathway
TNF works through two receptors, TNFR1 and TNFR2. TNFR1 is the major signal receptor of TNF-α. TNFR2, which mediates limited biological responses, binds to TNF-α and TNF-β. TNF signaling transduction through TNFR1 and TNFR2 can induce a variety of cellular responses, which depends on many factors, including the metabolic state of the cell and the adaptor proteins present in the cell.https://www.creativebiolabs.net/tnf-signaling-pathway.htm
TLR Pathway
Innate immune receptors, also known as pattern recognition receptors (PRRs), have been identified in the serum, on the cell surface, in endosomes, and in the cytoplasm. Toll-like receptors (TLRs) is one of the particularly important groups of PRRs.https://www.creativebiolabs.net/tlr-signal-pathway.htm
TGF-β Pathway
Transforming growth factor beta (TGF-β) is a cytokine that participates in both physiological and pathological processes.https://www.creativebiolabs.net/tgf-beta-signaling-pathway.htm
TCR Pathway
T-cell receptor (TCR) is a heterodimers composed of α and β peptide chains. TCR is mainly responsible for recognizing the antigens presented by major histocompatibility complex (MHC) molecules on the surface of antigen presenting cells (APC).https://www.creativebiolabs.net/tcr-signal-pathway.htm
RAS Pathway
Ras, which is a low-molecular-weight GDP/GTP-binding guanine triphosphatase, is the prototypical member of the Ras superfamily of proteins. https://www.creativebiolabs.net/ras-signaling-pathway.htm
PPAR Pathway
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors, which are responsible for regulating gene expression.https://www.creativebiolabs.net/ppar-signaling-pathway.htm
VPI3K-AKT Pathway
PI3K-Akt signaling pathway is one of the important signal transduction pathways in cells. It is involved in regulating cell metabolism, growth, proliferation, survival, transcription and protein synthesis by affecting the activation of downstream effector molecules. https://www.creativebiolabs.net/pi3k-akt-signaling-pathway.htm
More from Creative Biolabs (20)
Recently uploaded
Demystifying Fallopian Tube Blockage- Grading the Differences and Implication...
Fallopian tube blockage may cause female infertility. For treatment, herbal medicine Fuyan Pill can be a solution.
Ketone bodies and metabolism-biochemistry
This slide consists of all the topics of ketone . This can be used for exam purpose for writing about Diabetic keto acidosis etc . Thank you
Adhd Medication Shortage Uk - trinexpharmacy.com
The UK is currently facing a Adhd Medication Shortage Uk, which has left many patients and their families grappling with uncertainty and frustration. ADHD, or Attention Deficit Hyperactivity Disorder, is a chronic condition that requires consistent medication to manage effectively. This shortage has highlighted the critical role these medications play in the daily lives of those affected by ADHD. Contact : +1 (747) 209 – 3649 E-mail : sales@trinexpharmacy.com
The Nervous and Chemical Regulation of Respiration
These lecture slides, by Dr Sidra Arshad, offer a simplified look into the mechanisms involved in the regulation of respiration:
Learning objectives:
1. Describe the organisation of respiratory center
2. Describe the nervous control of inspiration and respiratory rhythm
3. Describe the functions of the dorsal and respiratory groups of neurons
4. Describe the influences of the Pneumotaxic and Apneustic centers
5. Explain the role of Hering-Breur inflation reflex in regulation of inspiration
6. Explain the role of central chemoreceptors in regulation of respiration
7. Explain the role of peripheral chemoreceptors in regulation of respiration
8. Explain the regulation of respiration during exercise
9. Integrate the respiratory regulatory mechanisms
10. Describe the Cheyne-Stokes breathing
Study Resources:
1. Chapter 42, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 36, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 13, Human Physiology by Lauralee Sherwood, 9th edition
Top Travel Vaccinations in Manchester
Travel vaccination in Manchester offers comprehensive immunization services for individuals planning international trips. Expert healthcare providers administer vaccines tailored to your destination, ensuring you stay protected against various diseases. Conveniently located clinics and flexible appointment options make it easy to get the necessary shots before your journey. Stay healthy and travel with confidence by getting vaccinated in Manchester. Visit us: www.nxhealthcare.co.uk
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
It includes regulation of combination products and medical devices. FDA and industry liaisons.
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotes
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
June 2024 Oncology Cartoons By Dr Kanhu Charan Patro
une 2024 Oncology Cartoons By Dr Kanhu Charan Patro
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa CentralClinic ^%[+27633867063*Abortion Pills For Sale In Tembisa CentralClinic ^%[+27633867063*Abortion Pills For Sale In Tembisa CentralClinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
NARCOTICS- POLICY AND PROCEDURES FOR ITS USE
This document outlines policies and procedures for handling narcotic and controlled drugs in NABH accredited hospitals.
Recently uploaded (20)
Demystifying Fallopian Tube Blockage- Grading the Differences and Implication...
Demystifying Fallopian Tube Blockage- Grading the Differences and Implication...
Muscles of Mastication by Dr. Rabia Inam Gandapore.pptx
Muscles of Mastication by Dr. Rabia Inam Gandapore.pptx
The Nervous and Chemical Regulation of Respiration
The Nervous and Chemical Regulation of Respiration
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
Tests for analysis of different pharmaceutical.pptx
Tests for analysis of different pharmaceutical.pptx
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotes
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotes
June 2024 Oncology Cartoons By Dr Kanhu Charan Patro
June 2024 Oncology Cartoons By Dr Kanhu Charan Patro
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
CHEMOTHERAPY_RDP_CHAPTER 6_Anti Malarial Drugs.pdf
CHEMOTHERAPY_RDP_CHAPTER 6_Anti Malarial Drugs.pdf
Fluorescent virus like particles design
- 1. Fluorescent Virus-Like Particles Design Reference ： https://www.creative-biolabs.com/vaccine/fluorescent-virus-like-particles-design.htm
- 2. CONTENTS 01 Why Use Fluorescent Virus-like Particles? 03 Genetic Fusion of Fluorophores to Proteins of VLPs 02 Chemical Labeling of Viral-like Particles
- 3. 0 1 Why Use Fluorescent Virus-like Particles? Fluorescent virus-like particles represent a convenient tool for characterization of virus-specific mechanisms and visualizing receptor-ligand interactions.
- 4. 0 1 Early research of viruses was accomplished using electron microscopy, of which the main disadvantage is that the cells to be analyzed must be immobilized and therefore cannot be applied to living cells, making kinetic studies difficult. 0 2 The growing variety of available fluorescent dyes and the development of fluorescence microscopy allows direct observation of the interaction between the entire virus and host cells. 0 3 The ability to locate and track viral proteins at the subcellular level lays a solid foundation for the development of real-time single-virus tracking methods within live cells.
- 5. Fig.1 Methods applied for the labeling of viral particles. (Wojta-Stremayr D, 2013)
- 6. 0 2 Chemical Labeling of Viral-like Particles The chemical compounds can be coupled to the viral particles either covalently or non-covalently. Chemical labeling can also be performed after the virus particles have already assembled.
- 7. 01 Covalent attachment: fluorophores containing amine-reactive groups. 03 Non-covalent attachment: lipophilic membrane dyes and dyes for the viral genome; biarsenical dyes.
- 8. PKH67 PKH26 DiD/Dil Octadecyl Rhodamine B Chloride (R18) [Ru(phen)2(dppz)]2+ Fluorescein Resurfin FITC Cy3 Cy5 CypHer5 Quantum dots Rhodamine derivates Alexa Fluor 488 Alexa Fluor 594 chemical labeling
- 9. 0 3 Genetic Fusion of Fluorophores to Proteins of VLPs By fusion of genes encoding viral and fluorescent proteins, a variety of different viruses can be fluorescently labeled, either DNA or RNA, enveloped or non-enveloped.
- 10. A Using this method, each particle carries the genetic information of the fluorescently labeled viral protein, thus each generation of viral progeny will also express the labeled protein. B Genetic labeling allows for the exploration of larger aspects of the viral life cycle as compared to chemical markers that allow for the study of only early events of viral infection. C Simultaneous labeling of several viral proteins with fluorescent proteins that emit light of different wavelengths allows tracking of the fate of such viral proteins after viral infection and during viral assembly.
- 11. THANK S