This document summarizes a study that used scanning electron microscopy to examine parasites found on wild giant freshwater prawns (Macrobrachium rosenbergii) in Malaysia. Researchers collected prawns from three locations and examined them for parasites. They discovered multiple specimens of Nematopsis sp., an apicomplexan protozoan, affecting the prawns. Scanning electron microscopy revealed details of the parasite's epimerite structure and striations, which are adaptations to increase surface area for nutrient intake from the host. The study aims to identify the Nematopsis species and further examine its life cycle.
Professor Wolf B. Frommer, Carnegie Institution for Science, Department of Plant, Biology, Stanford University. http://www.garvan.org.au/news-events/leaders-in-science-and-society
Professor Wolf B. Frommer, Carnegie Institution for Science, Department of Plant, Biology, Stanford University. http://www.garvan.org.au/news-events/leaders-in-science-and-society
DNA Barcode of a New Species Insect in Mangrove Ecosystem at Likupang Village...IJRES Journal
The research area at Mangrove forest in Likupang Village North Minahasa Regency, North Sulawesi Province, Indonesia. The aimed of this research are to document DNA barcode of insect inhabit in mangrove area. Insect were collected with sweep net and directly put in to the alcohol. The insect preparation for PCR (Polymerase Chain Reaction) at Basic Science Laboratory Sam Ratulangi University (MIPA faculty). The primer LCO1490: 5'-ggtcaacaaatcataaagatattgg-3' and HC02198: 5'-taaacttcagggtgaccaaaaaatca-3' at 710-bp. BLAST Analysis and the result barcode has ID :(lcl|Query_113365), 84% identification to species Gergithus iguchii also as the closer (0.20) distance organism, it means this organism as a new species, and has a taxonomy Order : Hemiptera, sub order : Neohemiptera, Super family :Fulgoridea, Family : Issidae.
Genetic Engineering By Ghawas khan from AWKUM PharmacyGhawas
Genetic Enginnearing is on one of the emerging branch of life sciences... Tried my best to create a best presentation for you. Remember me in your prayers.
Starvation and malnutrition are the major concerns in today’s world. According to FAO’s report, a total of 842 million people in 2011-13 were estimated to be suffering from chronic hunger, regularly not getting enough food to conduct an active life.
The total population of the world was 7.406 billion in 2012. The growth rate is 1.2% per year. Global cereal utilization for 2013-14 is projected to be 2,413 million tons, 3.2% higher than in 2012-13. Agricultural land is decreasing rapidly every year. Hence, more production in less agricultural area is necessary for feeding the whole lot of population.
The chapter written contributes towards the book published by OMICS USA for the book Progress in Biotechnology for Food Applications edited by Wing-Fu-Lai.
DNA Barcode of a New Species Insect in Mangrove Ecosystem at Likupang Village...IJRES Journal
The research area at Mangrove forest in Likupang Village North Minahasa Regency, North Sulawesi Province, Indonesia. The aimed of this research are to document DNA barcode of insect inhabit in mangrove area. Insect were collected with sweep net and directly put in to the alcohol. The insect preparation for PCR (Polymerase Chain Reaction) at Basic Science Laboratory Sam Ratulangi University (MIPA faculty). The primer LCO1490: 5'-ggtcaacaaatcataaagatattgg-3' and HC02198: 5'-taaacttcagggtgaccaaaaaatca-3' at 710-bp. BLAST Analysis and the result barcode has ID :(lcl|Query_113365), 84% identification to species Gergithus iguchii also as the closer (0.20) distance organism, it means this organism as a new species, and has a taxonomy Order : Hemiptera, sub order : Neohemiptera, Super family :Fulgoridea, Family : Issidae.
Genetic Engineering By Ghawas khan from AWKUM PharmacyGhawas
Genetic Enginnearing is on one of the emerging branch of life sciences... Tried my best to create a best presentation for you. Remember me in your prayers.
Starvation and malnutrition are the major concerns in today’s world. According to FAO’s report, a total of 842 million people in 2011-13 were estimated to be suffering from chronic hunger, regularly not getting enough food to conduct an active life.
The total population of the world was 7.406 billion in 2012. The growth rate is 1.2% per year. Global cereal utilization for 2013-14 is projected to be 2,413 million tons, 3.2% higher than in 2012-13. Agricultural land is decreasing rapidly every year. Hence, more production in less agricultural area is necessary for feeding the whole lot of population.
The chapter written contributes towards the book published by OMICS USA for the book Progress in Biotechnology for Food Applications edited by Wing-Fu-Lai.
Centre of innovation, Agricultural College and Research Institute,MaduraiSenthil Natesan
Establishment Central Instrumentation facility with the cost of 6.03 crore to take up multidisciplinary research project at AC&RI,Madurai. The analytical platform includes UP-HPLC for amino acid analysis, XRF for micronutrient analysis and GC-MS for metabolic profiling. The imaging facilities like upright, inverted and Florence microscope established for imaging pathogen & Insects. The molecular biology lab with real time PCR will help for the gene expression studies.
No fossil fuel
Low CO2 emissions
Dams can be used for flood control
may have benefits for tourism
long life, low maintenance costs
Pumped storage can help to meet variable demand
Breaks natural river flow
which affects fish,
sediment deposits
and downstream ecology
Dams cannot easily be removed, but gradually fill up with sediment
may displace people and affect livelihoods
Risk from earthquakes
water-borne diseases and other health problems
Diversity of fish and macrobenthos at the coastal area of Tanjung Kidurong Po...AbdullaAlAsif1
The regular assessment of faunal diversity near the energy power plant has gained the interest of many researchers. The activity and the effluent discharged from the power plant have remarkably stressed the adjacent assemblage of the alpha biotic community surrounding the power plant. Hence, this study aimed to assess the alpha faunal diversity of fishes and benthos in the bordering areas of Tanjung Kidurong Combined Cycle Power Plant (TKCCP), Bintulu Sarawak. The investigation was conducted in the coastal area from 2019 to 2020. Different ecological indices such as the evenness index, Margalef richness, and Shannon-Weiner diversity index were implemented, along with the cluster analysis of other stations. A number of 141 fish individuals were recorded, containing 40 species of 19 families. In addition, 13182 individuals of benthos communities from six classes and 37 species were identified. A percentage of 78 comprised fishes, while 22% were identified as crustaceans. Among the benthos addressed, Foraminifera was the major group (74%), followed by bivalve (10%) and Scaphopoda (7%). Compared to SEB2, 3 & 4, SEB1, the nearest to the power plant, recorded the highest richness, number of species, and diversity indexes. Cluster analysis, PCA, and nMDS analysis suggested that SEB2, SEB3, SEB4 had similar biodiversity compositions. The study of benthos indicates that SEB1 possesses a distinguished value of diversity and evenness indexes. This study would provide future researchers with vital documentation to evaluate the impact of thermal discharge from the power plant on alpha biodiversity in Bintulu, Sarawak, Malaysia.
Diversity of fish and macrobenthos at the coastal area of Tanjung Kidurong Po...
FCCHOONG EMSM2009
1. Scanning Electron Microscopy of
Apicomplexan Parasite Nematopsis sp.
Isolated from Wild Giant Freshwater Prawn,
Macrobrachium rosenbergii.
1Choong, F. C. and 2Kua, B. C.
1Institute of Tropical Aquaculture (AKUATROP), Universiti
Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu.
2National Fish Health Research Centre, Fisheries Research
Institute, 11960 Batu Maung, Penang, Malaysia.
18th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009 1
2. Giant Freshwater Prawn, M. rosenbergii
218th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
3. Giant Freshwater Prawn, M. rosenbergii
3
Main producer countries of Macrobrachium rosenbergii (FAO Fishery Statistics, 2006)
18th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
4. Giant Freshwater Prawn, M. rosenbergii
• Estimated production
– 246 tons (2007)
– RM 42- RM 54/kg
4418th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
5. Problems
5
• Limited seed supply
• Source:
– From wild
• Health status:
– unknown
5518th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
6. Objectives
• To screen the parasites found on wild giant
freshwater prawn, M. rosenbergii.
• To study the morphology of the parasites.
618th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
7. Sampling Site
18th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009 7
Teluk Intan, Perak
Sg. Timun, N. Sembilan
Kuching, Sarawak
8. 818th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
Methodology
Sampling on Wild Giant
Freshwater Prawn
Transport Live Sample
Back to Laboratory
Necropsy Procedures
10. Results & Discussion
0
2
4
6
8
10
12
14
16
18
N. Sembilan Perak Sarawak
Prevalence(%)
Prevalence of Apicomplexan Protozoan Infection in
Different Locations
1018th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
12. Nematopsis sp.
• Life cycle:
– Intermediate host: Molluscans
– Definitive host: Decapod
crustaceans
• Measurement:
– Length: 223.64 x 786.67 µm
– Width: 44.0 x 180.0 µm
1218th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
13. Image SEM image Reference Species Host of infection Location
-
Shanavas et
al., 1989
Nematopsis
rosenbergii
M. rosenbergii Kerala, India
-
Prasadan &
Janardanan,
1996
Nematopsis
idella
M. idella Kerala, India
Present
study
Species A M. rosenbergii
Perak, N.
Sembilan
Present
study
Species B M. rosenbergii
N. Sembilan,
Sarawak
Present
study
Species C M. rosenbergii Perak
18th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
Nematopsis sp.
16. Conclusion
• Epimerite:
– Apical complex
– Attach to the epithelial cell of the host
• Striation structure:
– Increase total surface area
– Increase efficiency of nutrition intake
18th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009 16
17. Future Study
• Identify the species of Nematopsis sp.
• Study the life cycle of current Nematopsis sp.
17171718th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009
18. Acknowledgements
• ABI Grant: Screening of the health status on parasitic
infections and IHHNV in wild M. rosenbergii.
• Mr. Omar & Mr. Rozman B Saleh
– Fishermen at sampling sites.
• Ms. Jamilah Afandi
– EM Unit, Universiti Sains Malaysia.
• National Fish Health Research Center, FRI, Penang.
• FRI Sarawak, Bintawa.
18th Electron Microscopy Society Malaysia Conference, UKM 15-17 Dec 2009 18
Editor's Notes
Epimerite: Apical complex – attach to epithelial cell of the host
