Eve Smith has extensive experience in cancer research, specializing in evaluating potential treatments for breast and pancreatic cancer. She has successfully collaborated on multiple projects, producing published work. Smith provides expertise in various laboratory techniques and has managed laboratories at Dana-Farber Cancer Institute and Massachusetts General Hospital.

1. EVE SMITH
Boston, MA eveyaffa@verizon.net (617) 731-5721
www.linkedin.com/in/eveyaffa
SUMMARY
A highly skilled research professional with extensive experience in evaluating the efficacy of multiple biochemical
compounds and oncolytic viruses for use as potential treatments in the areas of breast cancer and pancreatic cancer.
Successfully collaborated on many group projects; produced high quality data published in numerous research
articles. Assisted the Laboratory Manager in daily laboratory operations, including personnel training and lab
purchasing. Able to advance research projects, multitask and prioritize responsibilities.
Areas of Expertise:
 ELISA
 MTT Cell Proliferation assay
 Insitu Zymography
 Cell culture
 Cell migration and invasion assays
 Mini and Midi Plasmid DNA Purification
 Publications
 Immunohistochemistry
 Immunocytochemistry
 Cryostat and Microtome
 Upright Microscope
 Inverted Laser Scanning Confocal Microscope
PROFESSIONAL EXPERIENCE
DANA FARBER CANCER INSTITUTE, Boston, MA November 2015 – March 22, 2016
Dr. Dipanjan Chowdhury Laboratory/ Susan F. Smith Center
for Women’s Cancer
ResearchTechnician
 Assisted in processing ovarian cancer patients’ ascites fluid and freeze down the cells.
 Collected and compiled important information about the patient’s ascites fluid and cells.
 Performed a variety of cell culture and molecular biology techniques.
 Ordered the lab supplies for the lab.
MASSACHUSETTS GENERAL HOSPITAL, Boston, MA August 2004 – May 1st 2015
Edwin L. Steele Laboratory for Tumor Biology
ResearchTechnologist
 Assisted in the design and execution of experiments in the research group, leading to publications and funding of
grants.
 Performed a variety of cell culture techniques, in vitro experiments, ELISA, immunohistochemistry
(immunoperoxidase & immunofluorescence), and immunocytochemistry.
 Conducted image analysis of stained tissue sections and cells on inverted laser scanning confocal
and upright microscopes.
 Managed the cell culture room and trained new lab members in the proper cell culture techniques.
 Served as one of the primary professional resources in the areas of cell culture, microscope imaging, and
immunohistochemistry.
 Managed the Lab Cell Repository at one of the laboratory locations.
Career Transition – After Graduate School June 2003 – August 2004
ResearchAssistant – Graduate Student June 2000 – June 2003
 Assisted in the design and execution of research experiments by collecting
and performing a variety of hematological and coagulation techniques on patient samples.
 Collected and compiled necessary patient information for each experiment.
 Analyzed the data through statistical analysis and graphs, which lead to publications.

2. EVE SMITH eveyaffa@verizon.net page 2
Techniques & Automation
 Abnormal and normal differential blood smears
 CBC
 Erythrocyte sedimentation rate
 Heparin (anti-factor Xa) assay
 Heparin-Induced Thrombocytopenia ELISA assay
 Anti-prothrombin assay
 Extractable nuclear antigen immunoassay
 Manual cell counts
 Extractable nuclear antigen enzyme immunoassay
 Hematocrit and Hemoglobin
 FSP latex agglutination assay
 Anti-cardiolipin antibody assay
 Thrombin time and Reptilase Time
PUBLICATIONS
Daubriac J, Han S, Smith E, Hosein A, Buchanan M, Basik M, Boucher Y. The Crosstalk between Breast
Carcinoma –Associated Fibroblasts and Cancer Cells Promotes RhoA-Dependent Invasion via IGF-1 and PAI-1.
In submissions
Perentes JY, Kirkpatrick ND, Nagano S, Smith EY, Shaver CM, Sgroi D, Garkavtsev I, Munn LL, Jain RK,
Boucher Y. Cancer cell-associated MT1-MMP promotes vessel invasion and distant metastasis in
triple - negative mammary tumors. Cancer Res. 2011 Jul 1; 71(13): 4527-38
Smith EY, Charles LA, Van Cott EM. Biphasic Activated Partial Thromboplastin Time (aPTT) waveform
and Adverse Events in Non- Intensive Care Unit Patients. Am J Clin Pathol 2004; 121: 138-141
Van Cott E, Smith EY, Charles LA, Laposata M, Dew III RB. The Effects of Drotrecogin Alfa (Xigris) on the
Biphasic Activated Partial Thromboplastin Time (aPTT) waveform in Patients with Sepsis.
International Society on Thromobosis and Haemostasis XIX Congress and 49th Annual Scientific and
Standardization Committee Meeting. Birmingham England. July 12-18, 2003
J Thromb Haemost 2003; 1 suppliment 1. abstract #2768
Van Cott EM, Smith EY, Galanakis DK. Elevated Fibrinogen in an Acute Phase Reaction Prolongs
the Reptilase Time but Typically Not the Thrombin Time. Am J Clin Pathol 2002; 118:263-268
EDUCATION
Northeastern University, Boston, MA
Master of Science in Medical Laboratory Science
Thesis: “Suitability of the Thrombin Time Test for Monitoring Heparin Levels in Patients
with a Lupus Anticoagulant”
University of Hartford, West Hartford, CT
Bachelor of Science in Biology, magna cum laude
CLINICAL ROTATIONS
Hematology Clinical Rotation
Boston Medical Center, Boston, MA
 Trained to perform manual and automated hematology tests. Trained to perform automated coagulation tests.
Immunology Clinical Rotation
Brigham and Women’s Hospital, Boston, MA
 Trained and performed a variety of ELISA assays, allergy test, and other manual and automated immunology
tests.

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CERTIFICATION
Board Certified, American Society of Clinical Pathologists Board of Registry as a Technologist in Hematology
TECHNOLOGY
Excel, Word, PowerPoint, Adobe Acrobat, Filemaker, Fluoview Software, and Open Lab