Shambhavi Kumari submitted a project report to her university department of biotechnology on evaluating type 1 CRISPR gene silencing. The report provides an introduction to CRISPR as a bacterial defense system used for genome engineering and gene regulation. It describes the components of the CRISPR system, including Cas proteins, guide RNA, PAM sequences, and tracr RNA. The report also outlines Shambhavi's work at the Bhabha Atomic Research Centre under the guidance of Dr. Devashish Rath, as well as applications of CRISPR systems for genome editing, screening, therapeutics, and epigenetic editing.

1. Evaluation of Type 1 CRISPR System
based Gene Silencing
Project Work (Demo)
To
University Department of Biotechnology
By: Shambhavi Kumari
M.Sc Biotechnology 4th Semester
Project Work Carried Out
Under guidance of
Dr. DEVASHISH RATH
Scientific Officer –F
Molecular Biology Division
BHABHA ATOMIC RESEARCH CENTRE, MUMBAI
16/August/2020

2. Intoduction
• Clustered Regularly Interspaced Short Palindromi
c Repeats (or CRISPR) are a series of repetitive
sequences (exact repeats) interspaced by variable
sequences (the spacers)
• Originally a part of Bacterial defence system.
• New tools for genome engineering and gene
regulation, Genome editing tool.

3. Components of CRISPR System
• Different type of Cas proteins i.e., nuclease e,g
Cas 1, Cas2, Cas 3, Cas9 etc.
• Guide RNA or CrRNA
• PAM (Protospacer Adjacent Motifs)
tracr RNA
• Types :
The last published classification proposed up to five
types (from type I to V)

4. Wor

6. My Work at BARC

7. Application Of Crispr Sytem
• Genome editing.
• Using CRISPR libraries for screening.
• Therapeutic Applications.
• Transcriptional activation and repression.
• Epigenetic editing with CRISPR/Cas9.
• Live imaging of DNA/mRNA.