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CRISPR technology -A revolutionary discovery

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A superb technology that is going to change the future of molecular sciences and engineering..

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CRISPR technology -A revolutionary discovery

  1. 1. CRISPR TECHNOLOGY Guided by: Mr.Baji.K Presented by: P.Navaneeth Krishna Menon
  2. 2. INTRODUCTION • What is CRISPR Technology? • Clustered Regularly Interspaced Short Palindromic Repeats. • CRISPR/Cas system is used for genome editing. 2
  3. 3. CRISPR/Cas System • CRISPRs are segments of prokaryotic DNA containing short repetitions of base sequences. • CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as phages. • Much easier to design compared to its predecessors zinc finger nucleases and TALENs. 3
  4. 4. • Spacers are fragments of DNA gathered from the viruses that had tried to attack the cell. • CRISPR loci range from 24 to 48 bp. • CRISPR-associated(cas) genes are attached to the repeater-spacer array. 4
  5. 5. Action of CRISPR The CRISPR immune system works to protect bacteria from repeated viral attack via three basic steps: (1) Adaptation (2) Production of CRISPR RNA (3) Targeting 5
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  7. 7. APPLICATIONS In industry: CRISPR system are advantageous for industrial processes that utilize bacterial cultures. CRISPR-based immunity can be employed to make these cultures more resistant to viral attack, which would otherwise impede productivity. Eg: Streptococcus thermophilus, which is used to make yogurts and cheeses. 7
  8. 8. In the lab: 8
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  10. 10. IIn Medicine: Treatment of genetic diseases. To make more specific antibiotics that target only disease-causing bacterial strains while sparing beneficial bacteria. Researches are being carried out to make WBCs resistant to HIV infection. 10
  11. 11. CONCLUSION Jennifer Doudna ,Emmanuelle Charpentier and Feng Zhang 11
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  14. 14. REFERENCE • http://sitn.hms.harvard.edu/flash/2014/crispr-genetic- engineering-technique/ • Horvath P, Barrangou R (2010). "CRISPR/Cas, the immune system of bacteria and archaea". Science 327 (5962): 167–70 • https://www.addgene.org/crispr/guide/ • Sawyer E (9 February 2013). "Editing Genomes with the Bacterial Immune System".Scitable (blog). Nature Publishing Group. 14
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