A roadmap towards investing in agriculture, food security and nutrition. Presented at the Agriculture Nutrition Linkages Seminar in Dhaka, Bangladesh on the 18th of April, 2012.
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Erick Boy - Biofortified rice for Bangladesh: Plan, Findings and Directions
1. Biofortified Rice for
Bangladesh: Plan,
Findings and Directions
Erick Boy, MD, Ph.D. Nutrition Coordinator
HarvestPlus
April 18, 2012
HarvestPlus c/o IFPRI
2033 K Street, NW • Washington, DC 20006-1002 USA
Tel: 202-862-5600 • Fax: 202-467-4439
HarvestPlus@cgiar.org • www.HarvestPlus.org
2. Overview of rice zinc biofortification
project in Bangladesh
– Collaboration among plant scientists at BRRI and IRRI
and nutrition scientists at ICDDRB and UC Davis
– High-zinc rice cultivars back-crossed into local
varieties with desirable agronomic traits
– Potential impact on dietary zinc intake assessed in
young children and women of reproductive age
(dietary & Zn status survey)
– Zinc absorption from local diets containing
conventional and zinc-biofortified rice measured in
young children (Zn absorption studies)
– Measure impact on zinc status and function of
population (efficacy trial)
3. Nutrition Research
Consumption
Development Level
Estimate the Food Retention
Target Level
Bioavailability
Evaluation
Efficacy Trial
Effectiveness
Hotz & McClafferty,
2007
4. HarvestPlus Biofortified Rice
Preliminary nutrient target assumptions for parboiled
polished rice:
• Target Countries: Bangladesh & India
• Assumed average consumption/d: 400g (adult
woman) & 200 g (child 4-6 yr)
• Average baseline zinc content: 16 µg/g polished
• Retention (polished rice) after cooking: 90%
• Assumed absorption: 25%
• Estimated contribution to Daily Requirement
(EAR): 40%
• Increase by plant breeding: 8 µg/g
• Preliminary target: 24 µg/g
5. Dietary and Zn status survey
Cross-sectional survey in two sites,
with 2-stage cluster sampling
Pirgacha
Trishal
24 clusters per site, 10 HHs/cluster
Total 480 children ages 24-48 mo
• Weight, Height, serum zinc
• Food (rice & zinc) intake
• Zn & phytate in rice and lentils from
homes
6. Indicators of Zinc deficiency in Children (%)
25% low serum zinc
Prevalence Trishal Pirgacha
n=143 n=136
Stunted (< -2 64.3 48.3
HAZ)
Deficient 36 11
(<65 µg/dL)
Plasma zinc values adjusted using estimates from a regression model that included
indicators of elevated acute phase proteins and the time of day of blood sampling
7. Food sources of zinc (%)
Trishal Pirgacha
1 Rice 58.9 Rice 40.7
2 Fish 6.9 Dairy 7.2
3 Lentils 6.7 Egg 6.9
4 Dairy 5.0 Potato 4.7
5 Vegetables1 3.9 Fish 4.4
6 Egg 2.4 Lentils 4.4
7 Biscuit/cookie 2.2 Beef 4.4
8 Spices 1.8 Other legumes 3.6
9 Potato 1.8 Biscuit/cookie 3.2
10 Breastmilk 1.7 Vegetables1 3.2
1
vegetables other than potatoes or green leafy vegetables
8. Current and simulated
prevalence of inadequate zinc intakes
30 25.2
25
%
20 18 17
13.4 Trishal
15 Pirgacha
9.4
10 7.9
5
0
Current 35% 70%
Biofortified Rice Adoption (%)
Adequacy level is EAR of 3 mg zinc for 4-6 y old children (IZiNCG)
Data from Arsenault et al, J Nutr, 2010
9. Conclusions – dietary studies
Young children in Bangladesh have a high prevalence of zinc
deficiency
Rice is the primary source of dietary zinc
Increasing zinc content of rice will improve the adequacy of
zinc intakes in this population
Will increased zinc intake improve zinc status and health
outcomes?
10. Zinc absorption studies
Objective:
- Assess zinc absorption from local Bangladeshi diets
containing conventional or biofortified rice among young,
non-breast fed children
Measurements:
- Fractional zinc absorption (FZA), using triple isotope
ratio method
- Total zinc absorption (TZA) = dietary zinc * FZA
• Pilot study with IRRI elite line:
• Definitive study with BR Zinc biofortified Line: 2013
11. Pilot Study Methods – ICDDR,B and
University of California-Davis
• Within-child, cross-over design
• 42 children, 36-59 mo, WHZ>-2
• Conventional vs Biofortified vs Fortified
1.35 mg Zn/100 g unwashed, 2.40 mg Zn/100 g unwashed,
milled, raw (1.2 ppm washed) milled, raw (2.2 ppm washed)
12. IR68144: a serendipitous finding of the mid 1990s
IRRI elite line IR68144-3B-2-2-3, or IR68144 for short -- the
cross between cultivars IR72 and Zawa Bonday, selected for
their agronomic suitability for “cold elevated areas” and
aromatic quality. East Asian Science, Technology and Society: An International Journal (2011)
5:173–188
IR68144-3B-2-2-3, IRRI, Los Baños. Copyright: Michael Rubinstein,
International Food Policy Research Institute, 2003
13. Total dietary zinc (TDZ) by dietary
source and dietary period
Zn Intake by Group
Dietary component CR ZnBfR CR +Zn
Composite diet (mg/d)* 2.84 3.81 2.85
Zinc tracer + non- enriched 1.01 1.00 3.18
zinc (mg/d)*
Total measured zinc intake 3.81 4.81 6.03
(mg/d)
Estimated phytate : zinc 20 22 13
molar ratio
14. Total dietary zinc intake and fractional and
total zinc absorption from rice
CR ZnBfR CR +Zn p-
(n=20) value*
Total dietary zinc 3.81 4.81 6.03
intake (mg/d)
Fractional zinc 25.1 ± 4.1a 20.7 ± 4.2b 18.8 ± 3.4c <0.001
absorption (%)
Total absorbed 0.96 ±0.16a 1.0 ± 0.2a 1.13 ± <0.001
zinc (mg/d) 0.20b
*p-value for ANOVA comparing three dietary periods (two ZnBfR periods combined).
Dietary periods with different superscripts are significantly different, p<0.05.
15. Absorption from Pearl Millet Test Meals
Fractional
Group Absorption of Zn Absorbed Zn,
Age, mo (%) mg/d
Hi Iron/Zn 28 + 4 17.1 + 8.1 0.95 + 0.47
Low iron/Zn 29 + 3 20.2 + 4.2 0.67 + 0.24
P value 0.32 0.15 0.03
Personal communication. Hambidge M, B. Kodkani. 2012
A: 87 ug Zn/g millet; 9.4 mg phytate/g millet
B: 44 ug zn/g millet; 9.8 mg phytate/g millet
19. Planned amounts of dietary
components and zinc contents
Diet Component CR diet ZnBfR diet
Amt Zinc Amt Zinc
BR-28 (CR) 150 2.02 - -
IR-68144 (ZnBfR) - - 150 3.90
Lentils (as soup) 30 1.09 30 1.09
Fried green papaya 100 0.17 100 0.17
Zinc tracer 70
Zn 1.00 mg 67
Zn 1.00
mg
Total Zinc 4.28 6.16
20. Study protocol
Baseline fasting blood and
urines
Group A
1. Diet-ZnBfR + 67Zn
OR
2. Diet-CR + 70Zn
Group A
1. Diet-CR + 70Zn
OR
2. Diet-ZnBfR + 67Zn
Admit to IV 68Zn infusion, 4 hours
study ward after dinner
Discharge from study ward
Study days 1 2 3 4 5 6 7 8 9 10
Spot urines for Zn Isotope Ratios
Editor's Notes
Collaborative effort of…. Working with…. (rice) … to assess 1, 2, 3…
Here is the scheme of the nutrition research. In the development stages of the biofortified maize, the main question is by much do we need to increase the PVA levels. What is the target level? And to answer this question we need to know how much maize will people eat? How much of PVA is lost during the cooking process and how much stays in the food and how much is absorbed by the body. Then we will do an efficacy trial with to determine the impact of the PVA maize on the Vitamin A status of the study population under controlled conditions Effectiveness: when the PVA maize is made available to population to study the results on improved levels of vitA and health
This was a cross-sectional survey with 2-stage cluster sampling in 2 rural rice-growing regions of Bangladesh – Trishal and Pirgacha districts. In each site, there were 24 clusters with 10 households per cluster. The total sample size was 480 children ages 24-48 mo.
Since serum zinc is impacted by inflammatory status and the time of day of blood sampling, we adjusted the serum zinc values using estimates from a regression model that included indicators of elevated acute phase proteins (AGP and CRP) and the time of day of blood sampling. The mean serum zinc concentrations were 10 microgram per deciliter lower in Trishal than Pirgacha. Overall, 24% of children had low serum zinc concentrations, and the prevalence of low serum zinc concentrations was higher in Trishal.
These are the major food sources of zinc as a percentage of total daily zinc. Rice was the main source of zinc. There were low intakes of zinc from animal foods, especially in Trishal.
Adequacy of zinc intakes were assessed using an Estimated Average Requirement of 2 mg/d. Children in Trishal had a higher prevalence of inadequate zinc intakes – 25% vs. 18% in Pirgacha. If 35% of the children consumed zinc-biofortified rice, the prevalence of inadequacy decreased to ~ 17 and 13%; and if 70% of the children consumed zinc-biofortified rice, the prevalence of inadequacy decreased to about 8-9%.
Young children in Bangladesh have a high rate of zinc deficiency. Rice is the primary source of dietary zinc, and increasing the zinc content of rice will improve the adequacy of zinc intakes in this population. The next question is will increased zinc intakes improve zinc status and health outcomes? Future studies are underway to address these questions.
The primary aim of this study was to assess current dietary intakes of rice and zinc in rural rice-growing regions of Bangladesh as a first step in planning future studies for zinc-biofortified rice. With this baseline dietary intake data, we simulate the potential impact of increasing the zinc content of rice on the adequacy of zinc intakes of children. Specific aims were to assess anthropometric and zinc status of children, collect quantitative data on rice and total zinc intakes, assess adequacy of zinc intakes, and simulate zinc intakes if the rice zinc content were increased. In this study, the children were not consuming zinc-biofortified rice, this was a simulation exercise using the data on current rice and zinc intakes.
-The Case of Iron-Biofortified Rice Research in the Philippines .