This document outlines 10 unifying themes in the study of life. It discusses how biological systems function based on interactions between parts. It also explores the cellular basis of life, with cells specializing in different organisms. Structure and function are related, with form following functionality. Reproduction and inheritance allow transmission of genetic information between generations. All organisms interact with their environment to exchange materials and respond to changes. Energy is required for life and is obtained from chemical reactions and sunlight. Regulation allows organisms to maintain stable internal conditions. Evolution leads to diversity as organisms adapt over time. Scientific inquiry uses observation and experimentation to understand nature. Science, technology and society mutually influence each other to solve problems.