We at 3Brain are committed to advancing scientific research and boosting drug discovery. Like our technology, our product lines are always evolving to accommodate high-resolution recording of in vitro cultures. Discover our HD-MEA technology and soon-to-be-released devices and see how they are furthering research in brain diseases, drug discovery, retinal organoids, etc.. For more information, visit our website at https://www.3brain.com

1. Electrophysiological imaging
for advanced pharmacological screening

2. Imaging at different spatio-temporal scales
Macro- Meso- Micro-
100 billion neurons 2D/3D cultures, organoids,
tens of thousands of cells
Neurons and sub-elements

3. Competing technology
1.5mm
Main limitations
• temporal resolution
• field of view / spatial resolution
• indirect measurement: use of dyes
(averaging, bleaching, interference with
network parameters)
0.1mm
Main limitations:
• very low spatial resolution (electrode pitch
~100 μm)
• large under-sampling of network activity
(~20,000 neurons per 100 electrodes)
Optical imaging Extracellular electrophysiology

4. 3Brain’s BioCAM – BioChip Advantages
>0.5
0
Values[mV]
Spontaneous retinal waves of a P10 mouse under bicuculline effect
Standard MEAHigh-density MEA

5. Dissociated Cultures Acute Brain Tissue Retina Tissue

6. HD-MEA Probes
• 4,096 electrodes
• new 3D models coming
soon
BioCAM Line
• single- and multi- well
models
BrainWave 4
• data recording, analysis
and compression tools
Services
• data analysis, training,
protocols and
experimental design
Products and services
Solutions
Help
Care

7. Khíron chip
3D high-density multielectrode array – available from 2019 Q3

8. Khíron chip
3D high-density multielectrode array – available from 2019 Q3

9. Pharmaceutical applications
First 6-well prototype
coming soon
• Multi-well configuration
• Advanced data compression strategies
• Roadmap to increase # on wells: 12, 96, 384 and
more
From single-well… …to multi-well

10. Case studies
Dissociated cultures
Tissues

11. 24 Days In Vitro (DIV)
100nM
Oligomer Aβ-42
26 Hours of monitoring
H. Amin et al., Sci Report 2017
Dissociated hippocampal
neuronal network
In vitro model of induced Alzheimer’s disease

12. Human neuronal cultures on HD-MEA
validated for more than 3 months with
spontaneous and electrically evoked
recordings
Amin et al., Front. Neurosci. 2016
Several adhesion factors (PDLO,
PEI) promote different
maturation stages
Human-derived stem cell neuronal networks

13. Dissociated cultures
Tissues
Case studies

14. Epileptic model for anticonvulsant compound testing
Ferrea et al. 2012, Front. Neural Circuits
0.0
0.2
0.4
0.6
0.8
1.0
THIP
Control
CA1CA3DG
**
*
0.0
0.2
0.4
0.6
0.8
1.0
THIP
Control
CA1CA3DG
*
CLASS1
CLASS2
4-AP THIP 4-AP THIP

15. LTP protocols: D-Cycloserine effects on functional activity
A Maccione et al. – SFN 2016
LTP

16. Compound effect on purkinje activity in cerebellum slice (I)
Ca2+-activated K+
channels modulators
alter PCs firing

17. 0 10 20 30 40 50 60 70 80 900
50
200
150
100
Meannorm.frequency(%)
Time (min)
Basal
0 40 80 120
0
80
120
40
Basal (Hz)
Final(Hz)
Vehicle
Final
0 10 20 30 40 50 60 70 80 90
Time (min)
0 40 80 120
Basal (Hz)
CyPPA
0 10 20 30 40 50 60 70 80 90
Time (min)
0 40 80 120
Basal (Hz)
NS 309
0 10 20 30 40 50 60 70 80 90
Time (min)
0 40 80 120
Basal (Hz)
Apamin
A Ugolini et al. – Fens 2018
Compound effect on purkinje activity in cerebellum slice (II)

18. Light responsive human-derived
retinal organoids
D Hallam et al. – Stem Cells 2018