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International Journal of Horticulture, Agriculture and Food Science (IJHAF)
ISSN: 2456-8635
[Vol-6, Issue-2, Mar-Apr, 2022]
Issue DOI: https://dx.doi.org/10.22161/ijhaf.6.2
Article DOI: https://dx.doi.org/10.22161/ijhaf.6.2.2
https://aipublications.com/ijhaf/ Page | 7
Effect of application of “Grinophyll” on Growth and Yield
of Rice
S. S. Dekhane1
, P. B. Jadhav2
, D. J. Patel3
, A. A. Bhoir4
1&4
ASPEE, Agricultural Research and Development Foundation, At-Nare, Tal-Wada, Dist- Palghar, (MH) - 421312
2
Ex.Chief Agronomist, Farmsons Agri Solutions Pvt. Ltd., Nashik, (MH) – 422001
3
Ex. Principal and Dean, B. A. College of Agriculture, AAU, Anand (GJ) - 388 110
*
Corresponding author email: ardftansa@gmail.com
Received: 02 Mar 2022; Received in revised form: 29 Mar 2022; Accepted: 03 Apr 2022; Available online: 13 Apr 2022
©2022 The Author(s). Published by AI Publications. This is an open access article under the CC BY license
(https://creativecommons.org/licenses/by/4.0/)
Abstract— The present investigation entitled “effect of application of “Grinophyll” on growth and yield of
rice was carried out during kharif season of the year 2021 on the field of ASPEE, Agricultural Research
and Development Foundation, Tansa farm, At-Nare, Tal-Wada, Dist- Palghar, Maharashtra. The
experiment was laid out in Randomized Block Design. The three treatments (Control, Grinophyll @ 4ml,
and Grinophyll @6ml per 15 liters of water) were replicated twice times on four varieties of rice namely
Dandi, Gurjari, Jaya, and GAR-13. The plant population per meter square (34.5), plant height (105.9 cm),
number of tillers per plant (13.7), number of panicles per plant (13), number of panicles per square meter
(442) and length of panicle (25.8 cm) was recorded maximum with application of Grinophyll @ 6 ml /15
liters of water on rice cv. “GAR-13”. The highest number of seeds per panicle (203.5), test weight (34.1 g),
grain yield (57.45 q/ha) and straw yield (85.08 q/ha) was found with application of Grinophyll @ 6 ml / 15
liters of water.The data clearly revealed that, the yield obtained with treatment Grinophyll @ 6 ml / 15
liters of water on rice cv. “GAR 13” was significantly higher than all other treatments and also for growth
parameters.
Keywords— Grinophyll, Rice, Variety, Chlorophyll and Photosynthesis.
I. INTRODUCTION
Rice is the most prominent crop of India as it is the staple
food for most of the people of the country. This crop is the
backbone of livelihood for millions of rural households
and plays vital role in the country’s food security, so the
term “rice is life” is most appropriate in Indian context.
India occupies an important position both in area and
production of rice. By the adoption of improved
production technologies such as high-yielding
varieties/hybrids, expansion of irrigation potential, and use
of chemical fertilizer, supply of rice in the country has kept
pace with the increase in demand. Demand for rice is
expected to further increase in future as population is
continuously increasing, so production of rice also needs to
be increased. There is a need to further increase rice
productivity because land area under rice cultivation is
declining. Major constraints for productivity and
sustainability of rice-based systems in the country are the
inefficient use of inputs (fertilizer, water, labor), increasing
scarcity of water and labor especially for rice cultivation,
new emerging challenges from climate change, rising fuel
prices, increasing cost of cultivation, and socioeconomic
changes such as migration of labor, urbanization, less
liking for agricultural work by youths, and concerns from
environmental pollution. The only way to sustain rice
production for meeting the increasing population demand
is to increase the productivity per unit of area of rice with
enhanced resource use efficiency. For future productivity
gain in rice in India, high-yielding varieties that might
have resistance to multiple stresses (abiotic and biotic
stress) particularly in the wake of climate change need to
be explored. Crop production techniques in rice that could
increase factor productivity by efficient utilization of
inputs (water, fertilizers, pesticides, etc.) reduce cultivation
cost, enhance profit, and provide safe environment must be
explored. Encouraging resource conservation technologies
Dekhane et al. International Journal of Horticulture, Agriculture and Food Science (IJHAF)
6(2)-2022
https://aipublications.com/ijhaf/ Page | 8
and cultivation of climate-resilient high-yielding varieties
through demonstrations and making seed available to the
farmers will be important to sustain rice production in
India.
II. Materials and Methods
The experiment was conducted at ASPEE, Agricultural
Research and Development Foundation Farm, Village-
Nare, Tauka- Wada, District- Palghar in kharif season
during 2021 in Randomized Block Design (RBD) with 2
replications. The plot size was 19.5 m x 14.5 m. The
experimental site was located at 19.650
N latitudes and
73.130
E longitudes with average annual rainfall of 3600
mm. Four varietiescomprising 3 levels of Grinophyll were
tested in crop production. Gross plot size and net plot size
were 3.30 X 2.85 m and 3.15 X 2.80 m; respectively.
Planting was done on 15 July 2021 at spacing 20 X 15 cm.
There were 12 treatment combinations comprising viz.,
V1F1, V2F1, V3F1, V4F1, V1F2, V2F2, V3F2, V4F2,
V1F3, V2F3, V3F3 and V4F3were indicates Dandi,
Gujrati, Jaya and GAR-13varieties; respectively. F1, F2
and F3 were indicates 0 ml, 4 ml and 6 ml Grinophyll per
15 liters of water applied; respectively.
Data were compiled and analyzed using appropriate
statistical method. Keeping in view the above facts, the
present study was designed with the objective to study the
effect of Grinophyll on growth and yield attributes of four
transplanted rice varieties. Seedlings were prepared as per
recommended practices.
III. RESULTS AND DISCUSSION
The results in table 1 and Graph 1 indicated that different
treatments induced marked variations in number of
panicles per plant, number of panicles per square meter,
length of panicle, seeds per panicle, test weight, grain and
straw yields.
Plant growth parameters
The plant population per meter square (34.5), plant height
(105.9 cm), number of tillers per plant (13.7), number of
panicles per plant (13), number of panicles per square
meter (442) and length of panicle (25.8 cm) was found
maximum with application of Grinophyll @ 6 ml / 15
liters of water on rice cv. “GAR-13”.
Grinophyll help to increase chlorophyll concentration in
the plant leading to higher degree of photosynthesis. This
makes crops lush Green. It helps to enhancing rice
production (Matsuzakiet. al. 1980)
Yield parameters
The highest number of seeds per panicle (203.5), test
weight (34.1 g), grain yield (57.45 q/ha) and straw yield
(85.08 q/ha) was found with application of Grinophyll @ 6
ml / 15 liters of water.The data clearly revealed that, the
yield obtained with treatment Grinophyll @ 6 ml / 15 liters
of water) on rice cv. “GAR 13” was significantly higher
than all other treatments and also for growth parameters.
Yield attributes viz., seeds per panicle, test weight and
grain yield per panicle were significantly affected by
Grinophyll in rice. Grinophyllapplication increased the
number of spikelets per panicle of rice.This might be due
to increased synthesis of carbohydrates and that might
have increased the sink size and capacity (JunfeiGuet al.,
2017).
The contribution of carbohydrates from photosynthetic
activity for longer period might have resulted in efficient
translocation of food material into the sink (grain) thereby
increased the number of filled grains percentage.Grain
yield per panicle of rice also increased by Grinophyllthan
controls (Raj and Tripathi, 1999).
Grinophyll is a unique concentrated herbal formulation
which stimulates the process of chlorophyll formation in
leaves. This product is able to form chlorophyll in any
plant irrespective of scarcity of water and other climatic
variations. This product may be used separately or with
other products to improve chlorophyll content in leaf. It
relieves plant from stress conditions as well don’t have any
limitations as in the case of chemical fertilizers (Vermaet
al. 2004)
The grain weight increased with the increase in the
carbohydrate production in source. It helps to increase
transportation of carbohydrate from source to sink.(Arifet
al. 2012).
Table No. 1: Effect of application of “Grinophyll” on Growth and Yield of different Rice varieties under Konkan region.
Treatment
Plant
population
(per m²)
Plant
height
(cm)
No. of
tillers
per
plant
(Nos.)
No. of days to
50%
flowering(Days)
No. of
panicles
per plant
No. of
panicles(per
m²)
Length
of
panicle
(cm)
No. of
seeds
per
panicle
Test
weight
(g)
Grains
Yield
(q/ha)
Straw
Yield
(q/ha)
T1 31.5 99.4 9.2 74.8 9.1 300.3 22.2 117.8 17.7 39 57.9
T2 31.5 100.4 9.4 72.5 9.2 276 23 122 19.4 47 69.6
T3 32 101.3 10.2 72 9.4 291.4 23.3 123.1 20.4 48.6 72.1
Dekhane et al. International Journal of Horticulture, Agriculture and Food Science (IJHAF)
6(2)-2022
https://aipublications.com/ijhaf/ Page | 9
T4 32 101.5 10.8 76.5 9.9 336.6 23.7 124 23 52.9 78.4
T5 32.5 101.7 11.2 72.5 10.3 339.9 24.2 125.2 25.7 56 83
T6 33 103.1 11.5 73.3 10.9 370.6 24.5 131 29.1 57.7 85.5
T7 33 103.2 11.6 75.5 11.2 380.8 24.6 141 29.7 57.3 84.8
T8 33.5 103.7 11.6 79 11.5 379.5 24.8 145.7 32.1 58.2 86.3
T9 34 104.1 11.7 73.8 11.6 382.8 25 158.4 32.3 58.9 87.3
T10 34 105.2 12.3 69.8 11.7 397.8 25.1 171.1 32.5 63.8 94.5
T11 34 105.5 12.9 76.8 12.5 412.5 25.3 184.7 33 64.9 96.3
T12 34.5 105.9 13.7 77 13 442 25.8 203.5 34.1 68.6 101.7
S.Em(±) 1.14 0.31 0.30 2.27 0.28 9.03 0.26 11.05 1.2 4.77 7.08
CD NS 0.98 0.94 NS 0.88 28.1 0.79 34.39 3.72 14.86 22.02
NS – Non-Significant
Graph No. 1: Effect of application of “Grinophyll” on Growth, and Yield of different Rice varieties under Konkan region.
REFERENCES
[1] JunfeiGu, ZhenxiangZhou, ZhikangLi, YingChen,
ZhiqinWang, and HaoZhang, Rice (Oryza sativa L.) with
reduced chlorophyll content exhibit higher photosynthetic
rate and efficiency, improved canopy light distribution, and
greater yields than normally pigmented plants; Volume 200,
January 2017, Pages 58-70.
[2] Raj, A., and M. P. Tripathi, 1999: Relationship of leaf area
and chlorophyll content with yield in deep water Rice.
Indian Journal of Plant Physiology, 4, 219-220.
[3] Matsuzaki, A., K. Kariya, H. Machida and K. Tsunoda,
1980: Studies on the growth control and the nutrional
diagnosis in rice plants-1. The nutritional diagnosis based on
leaf colour and the estimation of number of spiklets per unit
area. Jpn. J. Crop Sci. 49, 439-444.
T1 T2 T3 T4 T5 T6
T7 T8 T9 T10 T11 T12
Dekhane et al. International Journal of Horticulture, Agriculture and Food Science (IJHAF)
6(2)-2022
https://aipublications.com/ijhaf/ Page | 10
[4] Raj, A., and M. P. Tripathi, 1999: Relation ship of leaf area
and chlorophyll content with yield in deep water rice. Indian
J. Plant Physiol. 4, 219Ð220
[5] Verma, A.K., Pandey, N. and Tripathi, R.S. (2004) Leaf
growth,chlorophyll, nitrogen content and grain yield of
hybrid riceas influenced by planting times and nitrogen
levels.Annals of Agricultural Research. 25(3): 456-458.
[6] Arif M, Shehzad MA, Bashir F, TasneemM,Yasi G and
Iqbal M (2012). Boron, zinc and microtone effects on
growth, chlorophyll contents and yield attributes in rice
(Oryza sativa L.) cultivar.African Journal of Biotechnology
11:10851-10858.
[7] Raj, A., and M. P. Tripathi, 1999: Relationship of leaf area
and chlorophyll content with yield in deep water rice. Indian
J. Plant Physiol. 4, 219Ð220
[8] Matsuzaki, A., K. Kariya, H. Machida, and K. Tsunoda,
1980: Studies on the growth contro

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Effect of application of “Grinophyll” on Growth and Yield of Rice

  • 1. International Journal of Horticulture, Agriculture and Food Science (IJHAF) ISSN: 2456-8635 [Vol-6, Issue-2, Mar-Apr, 2022] Issue DOI: https://dx.doi.org/10.22161/ijhaf.6.2 Article DOI: https://dx.doi.org/10.22161/ijhaf.6.2.2 https://aipublications.com/ijhaf/ Page | 7 Effect of application of “Grinophyll” on Growth and Yield of Rice S. S. Dekhane1 , P. B. Jadhav2 , D. J. Patel3 , A. A. Bhoir4 1&4 ASPEE, Agricultural Research and Development Foundation, At-Nare, Tal-Wada, Dist- Palghar, (MH) - 421312 2 Ex.Chief Agronomist, Farmsons Agri Solutions Pvt. Ltd., Nashik, (MH) – 422001 3 Ex. Principal and Dean, B. A. College of Agriculture, AAU, Anand (GJ) - 388 110 * Corresponding author email: ardftansa@gmail.com Received: 02 Mar 2022; Received in revised form: 29 Mar 2022; Accepted: 03 Apr 2022; Available online: 13 Apr 2022 ©2022 The Author(s). Published by AI Publications. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/) Abstract— The present investigation entitled “effect of application of “Grinophyll” on growth and yield of rice was carried out during kharif season of the year 2021 on the field of ASPEE, Agricultural Research and Development Foundation, Tansa farm, At-Nare, Tal-Wada, Dist- Palghar, Maharashtra. The experiment was laid out in Randomized Block Design. The three treatments (Control, Grinophyll @ 4ml, and Grinophyll @6ml per 15 liters of water) were replicated twice times on four varieties of rice namely Dandi, Gurjari, Jaya, and GAR-13. The plant population per meter square (34.5), plant height (105.9 cm), number of tillers per plant (13.7), number of panicles per plant (13), number of panicles per square meter (442) and length of panicle (25.8 cm) was recorded maximum with application of Grinophyll @ 6 ml /15 liters of water on rice cv. “GAR-13”. The highest number of seeds per panicle (203.5), test weight (34.1 g), grain yield (57.45 q/ha) and straw yield (85.08 q/ha) was found with application of Grinophyll @ 6 ml / 15 liters of water.The data clearly revealed that, the yield obtained with treatment Grinophyll @ 6 ml / 15 liters of water on rice cv. “GAR 13” was significantly higher than all other treatments and also for growth parameters. Keywords— Grinophyll, Rice, Variety, Chlorophyll and Photosynthesis. I. INTRODUCTION Rice is the most prominent crop of India as it is the staple food for most of the people of the country. This crop is the backbone of livelihood for millions of rural households and plays vital role in the country’s food security, so the term “rice is life” is most appropriate in Indian context. India occupies an important position both in area and production of rice. By the adoption of improved production technologies such as high-yielding varieties/hybrids, expansion of irrigation potential, and use of chemical fertilizer, supply of rice in the country has kept pace with the increase in demand. Demand for rice is expected to further increase in future as population is continuously increasing, so production of rice also needs to be increased. There is a need to further increase rice productivity because land area under rice cultivation is declining. Major constraints for productivity and sustainability of rice-based systems in the country are the inefficient use of inputs (fertilizer, water, labor), increasing scarcity of water and labor especially for rice cultivation, new emerging challenges from climate change, rising fuel prices, increasing cost of cultivation, and socioeconomic changes such as migration of labor, urbanization, less liking for agricultural work by youths, and concerns from environmental pollution. The only way to sustain rice production for meeting the increasing population demand is to increase the productivity per unit of area of rice with enhanced resource use efficiency. For future productivity gain in rice in India, high-yielding varieties that might have resistance to multiple stresses (abiotic and biotic stress) particularly in the wake of climate change need to be explored. Crop production techniques in rice that could increase factor productivity by efficient utilization of inputs (water, fertilizers, pesticides, etc.) reduce cultivation cost, enhance profit, and provide safe environment must be explored. Encouraging resource conservation technologies
  • 2. Dekhane et al. International Journal of Horticulture, Agriculture and Food Science (IJHAF) 6(2)-2022 https://aipublications.com/ijhaf/ Page | 8 and cultivation of climate-resilient high-yielding varieties through demonstrations and making seed available to the farmers will be important to sustain rice production in India. II. Materials and Methods The experiment was conducted at ASPEE, Agricultural Research and Development Foundation Farm, Village- Nare, Tauka- Wada, District- Palghar in kharif season during 2021 in Randomized Block Design (RBD) with 2 replications. The plot size was 19.5 m x 14.5 m. The experimental site was located at 19.650 N latitudes and 73.130 E longitudes with average annual rainfall of 3600 mm. Four varietiescomprising 3 levels of Grinophyll were tested in crop production. Gross plot size and net plot size were 3.30 X 2.85 m and 3.15 X 2.80 m; respectively. Planting was done on 15 July 2021 at spacing 20 X 15 cm. There were 12 treatment combinations comprising viz., V1F1, V2F1, V3F1, V4F1, V1F2, V2F2, V3F2, V4F2, V1F3, V2F3, V3F3 and V4F3were indicates Dandi, Gujrati, Jaya and GAR-13varieties; respectively. F1, F2 and F3 were indicates 0 ml, 4 ml and 6 ml Grinophyll per 15 liters of water applied; respectively. Data were compiled and analyzed using appropriate statistical method. Keeping in view the above facts, the present study was designed with the objective to study the effect of Grinophyll on growth and yield attributes of four transplanted rice varieties. Seedlings were prepared as per recommended practices. III. RESULTS AND DISCUSSION The results in table 1 and Graph 1 indicated that different treatments induced marked variations in number of panicles per plant, number of panicles per square meter, length of panicle, seeds per panicle, test weight, grain and straw yields. Plant growth parameters The plant population per meter square (34.5), plant height (105.9 cm), number of tillers per plant (13.7), number of panicles per plant (13), number of panicles per square meter (442) and length of panicle (25.8 cm) was found maximum with application of Grinophyll @ 6 ml / 15 liters of water on rice cv. “GAR-13”. Grinophyll help to increase chlorophyll concentration in the plant leading to higher degree of photosynthesis. This makes crops lush Green. It helps to enhancing rice production (Matsuzakiet. al. 1980) Yield parameters The highest number of seeds per panicle (203.5), test weight (34.1 g), grain yield (57.45 q/ha) and straw yield (85.08 q/ha) was found with application of Grinophyll @ 6 ml / 15 liters of water.The data clearly revealed that, the yield obtained with treatment Grinophyll @ 6 ml / 15 liters of water) on rice cv. “GAR 13” was significantly higher than all other treatments and also for growth parameters. Yield attributes viz., seeds per panicle, test weight and grain yield per panicle were significantly affected by Grinophyll in rice. Grinophyllapplication increased the number of spikelets per panicle of rice.This might be due to increased synthesis of carbohydrates and that might have increased the sink size and capacity (JunfeiGuet al., 2017). The contribution of carbohydrates from photosynthetic activity for longer period might have resulted in efficient translocation of food material into the sink (grain) thereby increased the number of filled grains percentage.Grain yield per panicle of rice also increased by Grinophyllthan controls (Raj and Tripathi, 1999). Grinophyll is a unique concentrated herbal formulation which stimulates the process of chlorophyll formation in leaves. This product is able to form chlorophyll in any plant irrespective of scarcity of water and other climatic variations. This product may be used separately or with other products to improve chlorophyll content in leaf. It relieves plant from stress conditions as well don’t have any limitations as in the case of chemical fertilizers (Vermaet al. 2004) The grain weight increased with the increase in the carbohydrate production in source. It helps to increase transportation of carbohydrate from source to sink.(Arifet al. 2012). Table No. 1: Effect of application of “Grinophyll” on Growth and Yield of different Rice varieties under Konkan region. Treatment Plant population (per m²) Plant height (cm) No. of tillers per plant (Nos.) No. of days to 50% flowering(Days) No. of panicles per plant No. of panicles(per m²) Length of panicle (cm) No. of seeds per panicle Test weight (g) Grains Yield (q/ha) Straw Yield (q/ha) T1 31.5 99.4 9.2 74.8 9.1 300.3 22.2 117.8 17.7 39 57.9 T2 31.5 100.4 9.4 72.5 9.2 276 23 122 19.4 47 69.6 T3 32 101.3 10.2 72 9.4 291.4 23.3 123.1 20.4 48.6 72.1
  • 3. Dekhane et al. International Journal of Horticulture, Agriculture and Food Science (IJHAF) 6(2)-2022 https://aipublications.com/ijhaf/ Page | 9 T4 32 101.5 10.8 76.5 9.9 336.6 23.7 124 23 52.9 78.4 T5 32.5 101.7 11.2 72.5 10.3 339.9 24.2 125.2 25.7 56 83 T6 33 103.1 11.5 73.3 10.9 370.6 24.5 131 29.1 57.7 85.5 T7 33 103.2 11.6 75.5 11.2 380.8 24.6 141 29.7 57.3 84.8 T8 33.5 103.7 11.6 79 11.5 379.5 24.8 145.7 32.1 58.2 86.3 T9 34 104.1 11.7 73.8 11.6 382.8 25 158.4 32.3 58.9 87.3 T10 34 105.2 12.3 69.8 11.7 397.8 25.1 171.1 32.5 63.8 94.5 T11 34 105.5 12.9 76.8 12.5 412.5 25.3 184.7 33 64.9 96.3 T12 34.5 105.9 13.7 77 13 442 25.8 203.5 34.1 68.6 101.7 S.Em(±) 1.14 0.31 0.30 2.27 0.28 9.03 0.26 11.05 1.2 4.77 7.08 CD NS 0.98 0.94 NS 0.88 28.1 0.79 34.39 3.72 14.86 22.02 NS – Non-Significant Graph No. 1: Effect of application of “Grinophyll” on Growth, and Yield of different Rice varieties under Konkan region. REFERENCES [1] JunfeiGu, ZhenxiangZhou, ZhikangLi, YingChen, ZhiqinWang, and HaoZhang, Rice (Oryza sativa L.) with reduced chlorophyll content exhibit higher photosynthetic rate and efficiency, improved canopy light distribution, and greater yields than normally pigmented plants; Volume 200, January 2017, Pages 58-70. [2] Raj, A., and M. P. Tripathi, 1999: Relationship of leaf area and chlorophyll content with yield in deep water Rice. Indian Journal of Plant Physiology, 4, 219-220. [3] Matsuzaki, A., K. Kariya, H. Machida and K. Tsunoda, 1980: Studies on the growth control and the nutrional diagnosis in rice plants-1. The nutritional diagnosis based on leaf colour and the estimation of number of spiklets per unit area. Jpn. J. Crop Sci. 49, 439-444. T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12
  • 4. Dekhane et al. International Journal of Horticulture, Agriculture and Food Science (IJHAF) 6(2)-2022 https://aipublications.com/ijhaf/ Page | 10 [4] Raj, A., and M. P. Tripathi, 1999: Relation ship of leaf area and chlorophyll content with yield in deep water rice. Indian J. Plant Physiol. 4, 219Ð220 [5] Verma, A.K., Pandey, N. and Tripathi, R.S. (2004) Leaf growth,chlorophyll, nitrogen content and grain yield of hybrid riceas influenced by planting times and nitrogen levels.Annals of Agricultural Research. 25(3): 456-458. [6] Arif M, Shehzad MA, Bashir F, TasneemM,Yasi G and Iqbal M (2012). Boron, zinc and microtone effects on growth, chlorophyll contents and yield attributes in rice (Oryza sativa L.) cultivar.African Journal of Biotechnology 11:10851-10858. [7] Raj, A., and M. P. Tripathi, 1999: Relationship of leaf area and chlorophyll content with yield in deep water rice. Indian J. Plant Physiol. 4, 219Ð220 [8] Matsuzaki, A., K. Kariya, H. Machida, and K. Tsunoda, 1980: Studies on the growth contro