This document provides an overview of diffusion, including its definition, occurrence in living and non-living systems, factors that affect its rate, and types in living systems. Diffusion is defined as the net movement of molecules from an area of high concentration to low concentration. The rate of diffusion depends directly on the concentration gradient and surface area, and inversely on the distance of the diffusion pathway. In living systems, passive diffusion is the simple diffusion of substances into or out of a cell without requiring energy.