Probiotics, prebiotics, enzymes, and activated charcoal are formulated in delayed release capsules to target delivery and promote beneficial effects in the host. Probiotics must survive the acidic gastric environment and reach the intestines in adequate amounts to confer health benefits. The delayed release capsules are designed to protect the ingredients from stomach acidity and delay release until the intestines, preserving potency. Clinical studies show the capsules successfully target delivery by delaying release and protecting ingredients from acid in the stomach.

1. Probiotics, Prebiotics, Enzymes & Activated Charcoal in
Delayed Release Capsules for Targeted & Controlled
Delivery
Divyanshu Raval
Technical Director & Partner
Walpar Healthcare
+91 7757870232

2. • In the world of highly processed food, particular attention is drawn to the composition and safety of consumed
products. The quality of food is very important because of, i.e., the root cause of food poisoning, obesity,
allergy, cardiovascular diseases, and cancer—the plague of the 21st century.
• So, to encounter such a dreadful situation in a natural way, plethora of scientific researches are happening
around the world with the probiotics & prebiotics to understand and harness their properties and now we have
ample of scientific reports that point to the health benefits of using probiotics and prebiotics in human
nutrition.
• In 2002, FAO (Food and Agriculture Organization of the United Nations) and WHO (World Health Organization)
working group experts, stated that probiotics are “live strains of strictly selected microorganisms which, when
administered in adequate amounts, confer a health benefit on the host”
• Again, in 2007, FAO/WHO experts described prebiotics as a nonviable food component that confers a health
benefit on the host associated with modulation of the microbiota.
• A helpful metaphor to understand the difference between a prebiotic and a probiotic may be a garden. You can
add seeds—the probiotic bacteria—while the prebiotic fiber is the water and fertilizer that helps the seeds to
grow and flourish.
• According to journal of current drug metabolism, the combination of digestive enzymes and probiotics may be
an interesting option for variety of intestinal problems.
• According to Kathleen McCoy, BS, activated charcoal helps promote a healthy digestive tract by removing
toxins that cause allergic reactions, oxidative damage and poor immune system function.

5. Working of Enzymes

12. Formulation and development
• Probiotics are live microorganisms that when present in sufficient amounts in the
digestive tract may confer health benefits on the host.
• To promote their beneficial effects in the host, probiotics must survive transit
through the harsh acidic conditions of gastric environment and being capable of
reaching the large intestine in adequate amounts to enable colonization and
proliferation.
• It has been recommended that food containing probiotic bacteria should be in the
range of 108–109 colony forming unit (cfu)/g right before ingestion to ensure that
sufficient therapeutic minimum of 106–107 cfu/g could reach the colon.
• Unfortunately, most of the probiotics lack the ability to survive in high quantity
because of low pH (pH = 2) in gastric juice and/or exposure to oxygen that limited
their effectiveness in most functional foods.6

13. Technology to render gastric fluid impotent to
degrade Active Ingredients
• Targeted (delayed) release capsules:
These capsule are designed to delay disintegration in acidic
environment of stomach (pH 1.2) & target the release in alkaline
environment of small intestine (pH 6.8) with a qualitative
composition comprising of strategically engineered acid resistant
polymers (HPMC), hydrocolloids (Gellan gum) and water,
thereby, preserve the potency and subsequent efficacy of
probiotics.

14. Graphical representation of In-vitro release
profile from targeted release capsule
The data shows that after 2 hours in pH 1.2 simulated gastric
fluids, less than 20 % of probiotics have dissolved and after 45
minutes in pH 6.8 simulated intestinal fluid more than 80 % of
active probiotics have dissolved, indicating that delayed release
capsules provide an acid resistant shield for probiotics.

15. Photographic representation of disintegration
profile from targeted release capsule
In pH 1.2 simulated gastric fluids, none of the six capsules had
disintegrated or opened. Again this indicates that delayed
release capsules are acid resistant.

16. Depiction of In-vivo targeted release profile from delayed
release capsule
As per the clinical study shown in above image, our specially designed
capsules are designed to target the capsule’s Ingredients to the specific
site of the body to function effectively by delaying the release.
Moreover, our capsules are protecting the capsule’s Ingredients from
stomach acidity.

17. References:
1. Paulina Markowiak * and Katarzyna ´ Sli˙zewska *, Effects of Probiotics,
Prebiotics, and Synbiotics on Human Health, Nutrients 2017, 9, 1021;
doi:10.3390/nu9091021.
2. https://www.prebiotin.com/prebiotin-academy/what-are-
prebiotics/prebiotics-vs-probiotics/
3. Jürgen Schrezenmeir and Michael de Vrese, Probiotics, prebiotics, and
synbiotics—approaching a definition1–3, Am J Clin Nutr
2001;73(suppl):361S–4S. Printed in USA. © 2001 American Society for
Clinical Nutrition
4. http://www.tmb.lth.se/fileadmin/_migrated/content_uploads/Synbiotic_pro
duc_extendedt.pdf
5. https://www.shutterstock.com/image-vector/functions-synbiotic-probiotic-
prebiotic-dysbacteriosis-infographics-770188102
6. Amal Bakr Shori, Microencapsulation Improved Probiotics Survival During
Gastric Transit, HAYATI Journal of Biosciences 24 (2017) 1e5.

18. Thank you so much