This document provides a summary of the professional experience and qualifications of Vernon L. Mar:
- Over 20 years of experience in biomedical research, including roles at Neumedicines, Inc., Amgen Inc., and the VA Medical Center, focusing on areas such as cancer biology, immunology, and vaccine development.
- Extensive experience with molecular biology techniques including cloning, protein expression and purification, and assays related to apoptosis, proliferation, and immunology.
- Key contributions include identifying biomarkers for IL-12 clinical trials, developing an enhanced tumor-directed IL-12 protein, and engineering a non-toxic pertussis toxoid vaccine as part of the team that cloned thrombopoietin.
This document provides a summary of Douglas Ivey's expertise and qualifications. Ivey has extensive experience in molecular discovery, genetics, and biochemistry. He has a Ph.D. in Microbiology and Molecular Genetics and has worked on projects involving drug screening, vaccine development, and identifying genetic mutations and proteins in various organisms. Ivey has strong technical skills and is well-suited for leading high-value research projects.
Radiation. Plant Toxicity. Inhibitors of radiation toxicity.Dmitri Popov
This document discusses cysteine proteases and their role in programmed cell death (PCD) in plants. It notes that vacuolar processing enzyme (VPE), a cysteine protease, has been identified as a key executor of PCD in plants, playing a role similar to caspases in animal apoptosis. VPE is involved in plant responses to stresses like radiation and pathogens by degrading vacuolar membranes and releasing hydrolytic enzymes to break down cellular components. The document examines the mechanisms and pathways of PCD in both animals and plants.
importance of pathogenomics in plant pathologyvinay ju
The document provides an outline for a seminar on pathogenomics for diagnosis and management of plant diseases. It includes sections on pathogenomics in plant pathology, diagnostic tools using next-generation sequencing technologies, host-microbe interaction and genes involved in virulence and resistance. The outline also lists various bioinformatics databases and molecular techniques used for pathogen detection, including PCR-based methods and microarrays. It discusses several examples of pathogenicity genes and host proteins involved in plant-virus interactions.
Recombinant DNA technology involves altering genetic material outside an organism to obtain desired traits. It has applications in improving human health by developing vaccines, insulin, diagnostics and more. In agriculture, genetically modified crops show increased yields, resistance to pests and tolerance of environmental stresses. The document discusses recent advances like CRISPR that allow precise genetic editing, and the roles of recombinant technologies in food production, medicine, the environment and energy. Overall, it presents recombinant DNA technology as having significantly improved human life through applications in health, food and the environment.
History
Host pathogen interaction
R gene
Molecular techniques for detection of plant pathogens
Role of molecular techniques in resistance breeding Deployment of R genes and linked markers
Transgenic approaches in plant protection
Conclusion
Toxicogenomics uses gene expression profiling to study how organisms respond to toxic compounds on a global scale. This new approach promises to greatly advance toxicology research. It may help identify toxic mechanisms earlier and assist in predicting compound toxicity. Challenges include interpreting large gene expression datasets and linking changes to specific toxic effects. Progress has been made using toxicogenomics to predict compound mode of action and toxicity pathways. Integrating gene expression data with traditional toxicology can help realize the full potential of this new approach.
Paternal radiation exposure led to increased expression of miR-29a and miR-29b in the male germline. This upregulation of miR-29 caused decreased expression of the de novo methyltransferase DNMT3a and profound hypomethylation of LINE1 and SINE B2 transposable elements in the germline. Hypomethylation of these transposable elements was also found in the thymus tissue of offspring conceived from exposed fathers and was associated with decreased expression of the LSH chromatin remodeling protein. Furthermore, miR-468 expression was significantly upregulated in the offspring thymus and directly targeted LSH, contributing to its decreased expression and hypomethylation of transposable
This document discusses a presentation on microbiome identification and characterization technologies. It begins with an introduction to the human microbiome and catalogs our "second genome". It then discusses how technologies like 16S rRNA sequencing and metagenomics have unlocked the ability to study the microbiome. Population studies of microbiome composition and disease associations are also reviewed. The presentation goes on to provide examples of how to design assays to identify and profile relevant microbiome targets, and discusses solutions for identification and profiling in microbiome research.
This document provides a summary of Douglas Ivey's expertise and qualifications. Ivey has extensive experience in molecular discovery, genetics, and biochemistry. He has a Ph.D. in Microbiology and Molecular Genetics and has worked on projects involving drug screening, vaccine development, and identifying genetic mutations and proteins in various organisms. Ivey has strong technical skills and is well-suited for leading high-value research projects.
Radiation. Plant Toxicity. Inhibitors of radiation toxicity.Dmitri Popov
This document discusses cysteine proteases and their role in programmed cell death (PCD) in plants. It notes that vacuolar processing enzyme (VPE), a cysteine protease, has been identified as a key executor of PCD in plants, playing a role similar to caspases in animal apoptosis. VPE is involved in plant responses to stresses like radiation and pathogens by degrading vacuolar membranes and releasing hydrolytic enzymes to break down cellular components. The document examines the mechanisms and pathways of PCD in both animals and plants.
importance of pathogenomics in plant pathologyvinay ju
The document provides an outline for a seminar on pathogenomics for diagnosis and management of plant diseases. It includes sections on pathogenomics in plant pathology, diagnostic tools using next-generation sequencing technologies, host-microbe interaction and genes involved in virulence and resistance. The outline also lists various bioinformatics databases and molecular techniques used for pathogen detection, including PCR-based methods and microarrays. It discusses several examples of pathogenicity genes and host proteins involved in plant-virus interactions.
Recombinant DNA technology involves altering genetic material outside an organism to obtain desired traits. It has applications in improving human health by developing vaccines, insulin, diagnostics and more. In agriculture, genetically modified crops show increased yields, resistance to pests and tolerance of environmental stresses. The document discusses recent advances like CRISPR that allow precise genetic editing, and the roles of recombinant technologies in food production, medicine, the environment and energy. Overall, it presents recombinant DNA technology as having significantly improved human life through applications in health, food and the environment.
History
Host pathogen interaction
R gene
Molecular techniques for detection of plant pathogens
Role of molecular techniques in resistance breeding Deployment of R genes and linked markers
Transgenic approaches in plant protection
Conclusion
Toxicogenomics uses gene expression profiling to study how organisms respond to toxic compounds on a global scale. This new approach promises to greatly advance toxicology research. It may help identify toxic mechanisms earlier and assist in predicting compound toxicity. Challenges include interpreting large gene expression datasets and linking changes to specific toxic effects. Progress has been made using toxicogenomics to predict compound mode of action and toxicity pathways. Integrating gene expression data with traditional toxicology can help realize the full potential of this new approach.
Paternal radiation exposure led to increased expression of miR-29a and miR-29b in the male germline. This upregulation of miR-29 caused decreased expression of the de novo methyltransferase DNMT3a and profound hypomethylation of LINE1 and SINE B2 transposable elements in the germline. Hypomethylation of these transposable elements was also found in the thymus tissue of offspring conceived from exposed fathers and was associated with decreased expression of the LSH chromatin remodeling protein. Furthermore, miR-468 expression was significantly upregulated in the offspring thymus and directly targeted LSH, contributing to its decreased expression and hypomethylation of transposable
This document discusses a presentation on microbiome identification and characterization technologies. It begins with an introduction to the human microbiome and catalogs our "second genome". It then discusses how technologies like 16S rRNA sequencing and metagenomics have unlocked the ability to study the microbiome. Population studies of microbiome composition and disease associations are also reviewed. The presentation goes on to provide examples of how to design assays to identify and profile relevant microbiome targets, and discusses solutions for identification and profiling in microbiome research.
This summarizes a study that developed a modified non-biotin polymerized horseradish peroxidase (HRP) immunohistochemical method for diagnosing canine distemper virus (CDV) infection from formalin-fixed tissue samples. The method confirmed CDV infection in seven of eight suspected cases. Labelled CDV antigen was observed in various tissues including brain, spinal cord, kidney, lungs, skin, and others. Compared to microwave pretreatment alone, autoclaving followed by microwave heating produced better labelling results. The non-biotin HRP detection system produced similar results to a conventional biotin-linked system.
1) Researchers developed transgenic medaka (fish) carrying multiple copies of a bacteriophage vector containing the cII gene, which serves as a target for detecting mutations.
2) They adapted an assay used in transgenic rodents to efficiently recover cII mutants from fish genomic DNA and detect them by infecting bacteria.
3) Spontaneous mutant frequencies in liver, whole fish, and testes of medaka were comparable to frequencies observed in transgenic rodents. Treatment with ethylnitrosourea induced cII mutations in the medaka in a tissue-specific and time-dependent manner consistent with known mutagen mechanisms.
This document summarizes several research grant proposals awarded by the International Centre for Genetic Engineering and Biotechnology (ICGEB) in 2019. The proposals cover a wide range of topics related to agriculture, health and biotechnology:
1) A proposal to study ferroptosis-like cell death in plants which occurs in response to heat stress and could lead to new approaches for crop protection during climate change.
2) Engineering vitamin A-rich orange eggplant which could help reduce vitamin A deficiency in developing countries.
3) Defining the soybean leaf microbiome to search for disease-suppressing bacteria against Asian Soybean Rust, a major threat to Brazilian soybean production.
4) Develop
Vasudevan Ayyappan is seeking new job opportunities and has over 10 years of experience in molecular biological techniques and 5+ years experience in next generation sequencing, genome editing, and pathway analysis. He has a broad range of skills in mammalian, microbial, and plant research and has effectively collaborated on multiple research projects.
Screening of receptor like kinase mutants of Arabidopsis thaliana using prote...Thomas Welch
This study screened 17 T-DNA knockout lines of receptor-like kinases in Arabidopsis thaliana for their response to protein extracts of the downy mildew pathogen Hyaloperonospora arabidopsidis using an oxidative burst assay. The assay found that knockout lines of ERL1 and BKK1 had significantly lower oxidative burst responses compared to the wild type, suggesting these genes may be involved in pathogen-associated molecular pattern triggered immunity against H. arabidopsidis. The results provide further evidence for the role of BKK1 in immunity and uncover the potential role of ERL1 in perceiving an obligate biotrophic pathogen, in contrast to previous research focusing on its response to nec
Microarray analysis allows scientists to determine which genes are active or inactive in a cell. It involves isolating genetic material from a cell and identifying which genes have messenger RNA present, indicating they are turned on. DNA microarrays contain thousands of unique DNA sequences spotted onto a solid surface that fluorescently-labeled genetic material from a sample can bind to. This allows scientists to measure gene expression levels across the entire genome simultaneously and understand molecular mechanisms of toxicity. Protein microarrays similarly contain arrays of capture proteins that can be used to analyze protein-protein interactions and activities on a large scale.
Washington Global Health Alliance Discovery Series
Robert Sinden, PhD
July 28, 2008
'Understanding Malaria Development in the Mosquito, and its Pivotal Role in the Formulation of Effective Control Strategies'
This document summarizes a research paper that studied the effects of a betasatellite associated with radish leaf curl disease (RaLCB) on the chloroplasts and photosynthesis of Nicotiana benthamiana. The key findings were:
1) The βC1 protein of RaLCB localized to the chloroplasts and caused damage to chloroplast ultrastructure.
2) RaLCB lacking the βC1 protein did not affect chloroplast structure or cause symptoms.
3) Expression of βC1 alone was able to induce vein flecking symptoms associated with chloroplast damage.
4) RaLCB infection down-regulated genes involved in chlorophyll biosynthesis and chloroplast development, reducing photosynthetic efficiency
This document discusses the field of metagenomics, which involves directly extracting and sequencing genetic material from environmental samples without culturing individual microbial species. It provides a brief history of metagenomics from early microbiologists in the 17th century to recent large-scale sequencing projects. Methods of metagenomic analysis like sequence-driven and function-driven approaches are described. Applications to studying uncultured symbiotic microbes, extreme environments, and the human gut microbiome are also summarized.
This curriculum vitae outlines the educational and professional background of Julio A. Urbina R. He received a Licenciado in Biology from the Central University of Venezuela in 1970 and a Ph.D. in Chemistry from the Massachusetts Institute of Technology in 1975. His positions have included professorships at the University of Cincinnati and Central University of Venezuela. He has also held various research positions at the Venezuelan Institute for Scientific Research since 1991. The CV lists over 40 publications in peer-reviewed journals related to biological chemistry and parasitology research.
This document describes an experiment to detect the presence of genetically modified organisms (GMOs) in various food items using polymerase chain reaction (PCR) and gel electrophoresis. Samples tested included papaya, soy milk, wheat, and corn. It was hypothesized that soy milk and corn would test positive for GMOs, while papaya and wheat would not. DNA was extracted from the samples and tested using PCR with two different master mixes. The PCR products were run on a gel electrophoresis to detect bands indicating the presence of the CaMV35S and NOS genes commonly found in GMOs. The results were inconclusive due to possible errors in technique. Repeating the experiment with improved methodology was proposed.
In fungal genomes, chromosomal polymorphisms resulting from chromosomal rearrangements (CRs) are widely documented. Genome plasticity and chromosomal rearrangements among and between fungal genomes fundamentally changed the view of scientists to understand more in-depth on genome behavior during sexual and asexual processes. Earlier, to find variability, conventional light microscopy and germ tube burst methods were the only approachs to visualize fungal chromosomes through staining with standard dyes such as giemsa or aceto-orceine. Eventually, techniques like Pulsed-field gel electrophoresis (PFGE) and next-generation sequencing became a significant tipping point for many aspects of fungal molecular biology including CR research (Mehrabi et al., 2017).
Single Cell Insights: Studying Environmental Microbial Communities Cell by CellQIAGEN
Dissecting complex microbial communities has incredible potential in the quest to decipher the world around us and find new sources of enzymes, antibiotics and other drugs. The challenge that remains is how to gain a deeper understanding of the roles and interactions of individual microbes. Single cell sequencing has emerged as an innovative investigational approach that provides a view of the cell-by-cell community by separating out individual microbes prior to sequencing. This issue of Single Cell Insights reviews peer-reviewed journal articles that describe applications and methodologies for single cell sequencing of environmental microbial communities.
Microbiome Identification to Characterization: Pathogen Detection Webinar Ser...QIAGEN
This document discusses the development of rapid detection methods for microbial and microbiome analysis and their applications to human health. It provides an overview of QIAGEN's microbial qPCR products and discusses focused metagenomics applications like screening for antibiotic resistance genes in the food supply and human gut. Limitations of current methods are outlined and the benefits of qPCR for rapid, specific, and sensitive microbial detection are described.
1) The C-terminal carboxylate group of integrin β1 is necessary for its association with the kindlin-2 adapter protein. Affinity measurements indicate this interaction is coordinated by a putative carboxylate-binding motif in the FERM subdomain F3 of kindlin-2.
2) Injection of mRNA encoding mutant integrin β1 cytoplasmic tails that lack the C-terminal carboxylate group perturbed laterality organ development in zebrafish, indicating the carboxylate group is required for physiological functions.
3) The unusual interaction between integrin β1 and kindlin-2 identified here represents a novel protein-protein interaction mode governed mainly by the integrin
Biotransformation through genetic engineeringsweety parmar
This document discusses using genetic engineering to modify terpenoid metabolism in plants. It describes how plants release terpenoid volatiles when damaged by herbivores to attract carnivorous enemies. The document outlines engineering plant mitochondria to overexpress a sesquiterpene synthase gene from mint, which increased production of the volatile (E)-nerolidol to attract more carnivores. It also discusses genome editing techniques like zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9 for applications such as correcting the mutation that causes sickle cell disease.
RNA-guided Cas9-induced mutagenesis in tobaccoStefanie Pencs
This study investigated the heritability of CRISPR/Cas9-induced mutations in tobacco. The researchers targeted the GFP gene in transgenic tobacco plants using a GFP-specific guide RNA and Cas9. They found that 80% of transformed (T0) plants had single or multiple mutations in GFP. About half of the mutations proved heritable through self-fertilization. The researchers also regenerated plants from cultured embryogenic pollen and leaf explants of mutated plants. They found that embryogenic pollen culture allowed for more efficient production of homozygous mutants compared to sexual reproduction. One mutation recovered through vegetative propagation was later found to be heritable upon self-fertilization of the clones. The study demonstrated
This document discusses biotechnology and genetic engineering. It provides examples of how biotechnology is used in forensics, agriculture, and genetic engineering. Genetic engineering involves transferring genes between organisms, such as placing human genes in bacteria. The document also discusses applications of genetic engineering like creating pest-resistant and herbicide-resistant crops through biotechnology techniques.
This document summarizes a study analyzing genetic variability in two beta-tubulin genes (isotypes 1 and 2) in the parasitic nematode Haemonchus contortus. The study compared alleles at these loci between a benzimidazole-susceptible strain and two independently derived benzimidazole-resistant strains. Both resistant strains showed significant shifts in allele frequencies at both loci compared to the susceptible strain, with the same alleles favored in both resistant strains. This suggests selection by benzimidazoles acted on both loci to confer resistance. The results provide evidence that changes in allele frequencies, rather than novel genetic changes, explain the development of benzimidazole resistance.
Raju Ramdas Jadhav has over 7 years of experience managing workforce and operations for contact centers. He currently works as a Senior Analyst for workforce management and management information systems at Concentrix Services India Private Limited, where he is responsible for program management, forecasting, analysis, reporting, and decision making. Previously, he worked as a Senior Customer Support Associate for an escalation desk. He has strong communication, analytical, and people management skills.
REVIEW ON SECRET IMAGE SHARING USING QR CODE GENERATION TECHNICpriyanka singh
The document summarizes a research paper on embedding a secret watermark image into a QR code image using wavelet transform. It discusses converting a binary logo image into a watermark and inserting it into a selected sub-band of the QR code after decomposing the image using two-level two-dimensional wavelet transform. The watermark can then be extracted from the watermarked QR code without needing the original image by using the predicted values and secret key to determine the embedded bit. Previous digital watermarking techniques are also briefly reviewed, including spatial and frequency domain methods.
This summarizes a study that developed a modified non-biotin polymerized horseradish peroxidase (HRP) immunohistochemical method for diagnosing canine distemper virus (CDV) infection from formalin-fixed tissue samples. The method confirmed CDV infection in seven of eight suspected cases. Labelled CDV antigen was observed in various tissues including brain, spinal cord, kidney, lungs, skin, and others. Compared to microwave pretreatment alone, autoclaving followed by microwave heating produced better labelling results. The non-biotin HRP detection system produced similar results to a conventional biotin-linked system.
1) Researchers developed transgenic medaka (fish) carrying multiple copies of a bacteriophage vector containing the cII gene, which serves as a target for detecting mutations.
2) They adapted an assay used in transgenic rodents to efficiently recover cII mutants from fish genomic DNA and detect them by infecting bacteria.
3) Spontaneous mutant frequencies in liver, whole fish, and testes of medaka were comparable to frequencies observed in transgenic rodents. Treatment with ethylnitrosourea induced cII mutations in the medaka in a tissue-specific and time-dependent manner consistent with known mutagen mechanisms.
This document summarizes several research grant proposals awarded by the International Centre for Genetic Engineering and Biotechnology (ICGEB) in 2019. The proposals cover a wide range of topics related to agriculture, health and biotechnology:
1) A proposal to study ferroptosis-like cell death in plants which occurs in response to heat stress and could lead to new approaches for crop protection during climate change.
2) Engineering vitamin A-rich orange eggplant which could help reduce vitamin A deficiency in developing countries.
3) Defining the soybean leaf microbiome to search for disease-suppressing bacteria against Asian Soybean Rust, a major threat to Brazilian soybean production.
4) Develop
Vasudevan Ayyappan is seeking new job opportunities and has over 10 years of experience in molecular biological techniques and 5+ years experience in next generation sequencing, genome editing, and pathway analysis. He has a broad range of skills in mammalian, microbial, and plant research and has effectively collaborated on multiple research projects.
Screening of receptor like kinase mutants of Arabidopsis thaliana using prote...Thomas Welch
This study screened 17 T-DNA knockout lines of receptor-like kinases in Arabidopsis thaliana for their response to protein extracts of the downy mildew pathogen Hyaloperonospora arabidopsidis using an oxidative burst assay. The assay found that knockout lines of ERL1 and BKK1 had significantly lower oxidative burst responses compared to the wild type, suggesting these genes may be involved in pathogen-associated molecular pattern triggered immunity against H. arabidopsidis. The results provide further evidence for the role of BKK1 in immunity and uncover the potential role of ERL1 in perceiving an obligate biotrophic pathogen, in contrast to previous research focusing on its response to nec
Microarray analysis allows scientists to determine which genes are active or inactive in a cell. It involves isolating genetic material from a cell and identifying which genes have messenger RNA present, indicating they are turned on. DNA microarrays contain thousands of unique DNA sequences spotted onto a solid surface that fluorescently-labeled genetic material from a sample can bind to. This allows scientists to measure gene expression levels across the entire genome simultaneously and understand molecular mechanisms of toxicity. Protein microarrays similarly contain arrays of capture proteins that can be used to analyze protein-protein interactions and activities on a large scale.
Washington Global Health Alliance Discovery Series
Robert Sinden, PhD
July 28, 2008
'Understanding Malaria Development in the Mosquito, and its Pivotal Role in the Formulation of Effective Control Strategies'
This document summarizes a research paper that studied the effects of a betasatellite associated with radish leaf curl disease (RaLCB) on the chloroplasts and photosynthesis of Nicotiana benthamiana. The key findings were:
1) The βC1 protein of RaLCB localized to the chloroplasts and caused damage to chloroplast ultrastructure.
2) RaLCB lacking the βC1 protein did not affect chloroplast structure or cause symptoms.
3) Expression of βC1 alone was able to induce vein flecking symptoms associated with chloroplast damage.
4) RaLCB infection down-regulated genes involved in chlorophyll biosynthesis and chloroplast development, reducing photosynthetic efficiency
This document discusses the field of metagenomics, which involves directly extracting and sequencing genetic material from environmental samples without culturing individual microbial species. It provides a brief history of metagenomics from early microbiologists in the 17th century to recent large-scale sequencing projects. Methods of metagenomic analysis like sequence-driven and function-driven approaches are described. Applications to studying uncultured symbiotic microbes, extreme environments, and the human gut microbiome are also summarized.
This curriculum vitae outlines the educational and professional background of Julio A. Urbina R. He received a Licenciado in Biology from the Central University of Venezuela in 1970 and a Ph.D. in Chemistry from the Massachusetts Institute of Technology in 1975. His positions have included professorships at the University of Cincinnati and Central University of Venezuela. He has also held various research positions at the Venezuelan Institute for Scientific Research since 1991. The CV lists over 40 publications in peer-reviewed journals related to biological chemistry and parasitology research.
This document describes an experiment to detect the presence of genetically modified organisms (GMOs) in various food items using polymerase chain reaction (PCR) and gel electrophoresis. Samples tested included papaya, soy milk, wheat, and corn. It was hypothesized that soy milk and corn would test positive for GMOs, while papaya and wheat would not. DNA was extracted from the samples and tested using PCR with two different master mixes. The PCR products were run on a gel electrophoresis to detect bands indicating the presence of the CaMV35S and NOS genes commonly found in GMOs. The results were inconclusive due to possible errors in technique. Repeating the experiment with improved methodology was proposed.
In fungal genomes, chromosomal polymorphisms resulting from chromosomal rearrangements (CRs) are widely documented. Genome plasticity and chromosomal rearrangements among and between fungal genomes fundamentally changed the view of scientists to understand more in-depth on genome behavior during sexual and asexual processes. Earlier, to find variability, conventional light microscopy and germ tube burst methods were the only approachs to visualize fungal chromosomes through staining with standard dyes such as giemsa or aceto-orceine. Eventually, techniques like Pulsed-field gel electrophoresis (PFGE) and next-generation sequencing became a significant tipping point for many aspects of fungal molecular biology including CR research (Mehrabi et al., 2017).
Single Cell Insights: Studying Environmental Microbial Communities Cell by CellQIAGEN
Dissecting complex microbial communities has incredible potential in the quest to decipher the world around us and find new sources of enzymes, antibiotics and other drugs. The challenge that remains is how to gain a deeper understanding of the roles and interactions of individual microbes. Single cell sequencing has emerged as an innovative investigational approach that provides a view of the cell-by-cell community by separating out individual microbes prior to sequencing. This issue of Single Cell Insights reviews peer-reviewed journal articles that describe applications and methodologies for single cell sequencing of environmental microbial communities.
Microbiome Identification to Characterization: Pathogen Detection Webinar Ser...QIAGEN
This document discusses the development of rapid detection methods for microbial and microbiome analysis and their applications to human health. It provides an overview of QIAGEN's microbial qPCR products and discusses focused metagenomics applications like screening for antibiotic resistance genes in the food supply and human gut. Limitations of current methods are outlined and the benefits of qPCR for rapid, specific, and sensitive microbial detection are described.
1) The C-terminal carboxylate group of integrin β1 is necessary for its association with the kindlin-2 adapter protein. Affinity measurements indicate this interaction is coordinated by a putative carboxylate-binding motif in the FERM subdomain F3 of kindlin-2.
2) Injection of mRNA encoding mutant integrin β1 cytoplasmic tails that lack the C-terminal carboxylate group perturbed laterality organ development in zebrafish, indicating the carboxylate group is required for physiological functions.
3) The unusual interaction between integrin β1 and kindlin-2 identified here represents a novel protein-protein interaction mode governed mainly by the integrin
Biotransformation through genetic engineeringsweety parmar
This document discusses using genetic engineering to modify terpenoid metabolism in plants. It describes how plants release terpenoid volatiles when damaged by herbivores to attract carnivorous enemies. The document outlines engineering plant mitochondria to overexpress a sesquiterpene synthase gene from mint, which increased production of the volatile (E)-nerolidol to attract more carnivores. It also discusses genome editing techniques like zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9 for applications such as correcting the mutation that causes sickle cell disease.
RNA-guided Cas9-induced mutagenesis in tobaccoStefanie Pencs
This study investigated the heritability of CRISPR/Cas9-induced mutations in tobacco. The researchers targeted the GFP gene in transgenic tobacco plants using a GFP-specific guide RNA and Cas9. They found that 80% of transformed (T0) plants had single or multiple mutations in GFP. About half of the mutations proved heritable through self-fertilization. The researchers also regenerated plants from cultured embryogenic pollen and leaf explants of mutated plants. They found that embryogenic pollen culture allowed for more efficient production of homozygous mutants compared to sexual reproduction. One mutation recovered through vegetative propagation was later found to be heritable upon self-fertilization of the clones. The study demonstrated
This document discusses biotechnology and genetic engineering. It provides examples of how biotechnology is used in forensics, agriculture, and genetic engineering. Genetic engineering involves transferring genes between organisms, such as placing human genes in bacteria. The document also discusses applications of genetic engineering like creating pest-resistant and herbicide-resistant crops through biotechnology techniques.
This document summarizes a study analyzing genetic variability in two beta-tubulin genes (isotypes 1 and 2) in the parasitic nematode Haemonchus contortus. The study compared alleles at these loci between a benzimidazole-susceptible strain and two independently derived benzimidazole-resistant strains. Both resistant strains showed significant shifts in allele frequencies at both loci compared to the susceptible strain, with the same alleles favored in both resistant strains. This suggests selection by benzimidazoles acted on both loci to confer resistance. The results provide evidence that changes in allele frequencies, rather than novel genetic changes, explain the development of benzimidazole resistance.
Raju Ramdas Jadhav has over 7 years of experience managing workforce and operations for contact centers. He currently works as a Senior Analyst for workforce management and management information systems at Concentrix Services India Private Limited, where he is responsible for program management, forecasting, analysis, reporting, and decision making. Previously, he worked as a Senior Customer Support Associate for an escalation desk. He has strong communication, analytical, and people management skills.
REVIEW ON SECRET IMAGE SHARING USING QR CODE GENERATION TECHNICpriyanka singh
The document summarizes a research paper on embedding a secret watermark image into a QR code image using wavelet transform. It discusses converting a binary logo image into a watermark and inserting it into a selected sub-band of the QR code after decomposing the image using two-level two-dimensional wavelet transform. The watermark can then be extracted from the watermarked QR code without needing the original image by using the predicted values and secret key to determine the embedded bit. Previous digital watermarking techniques are also briefly reviewed, including spatial and frequency domain methods.
This document discusses how the HIV/AIDS epidemic in sub-Saharan Africa affects gender inequality for women. It notes that women make up 60% of people living with HIV in the region due to various biological and sociocultural factors that increase women's susceptibility. These factors include female genital mutilation, lack of education limiting economic opportunities, expectation of abstinence but not for husbands, and polygamy allowing spread of the virus between wives. While the epidemic initially seemed to undermine progress on gender equality, it also increased recognition of women's rights and needs.
Principis i polítiques de salut
Elements d'entorn
Les xifres dels pressupostos
La millor política de salut és la lluita contra les desigualtats
i la construcció d’un estat del benestar socialment avançat
A rupture in a Ф52" pipe upstream of a bend at the Tesoro Refinery on April 2, 2010 caused six fatalities. An investigation found the rupture was due to thinning of the pipe wall and over pressurization, which are symptoms of high temperature sulfidation and CLSCC (corrosion under low stress conditions). The document warns that a focus on quick fixes based only on symptoms without conducting a root cause analysis, fast repairs just to resume production, and rewarding staff for attending to problems in a reactive manner can develop a reactive staff culture and reactive role models. This type of culture is prone to catastrophic failures.
Hao Liu has over 15 years of experience in drug discovery research. He has developed biochemical and cell-based assays to screen for oncology and metabolic disease targets. He is skilled in developing and optimizing high throughput screening assays, cell signaling studies, and molecular biology techniques. Liu has authored several publications and presented at conferences.
This document provides a summary of Dennis Allen Sheeter's education and work experience. He received a Ph.D. in Molecular Pathology from UC San Diego in 2002. Since then, he has held various positions in academia and industry, including as an advanced science instructor, independent scientific consultant, and research fellow. He has also published 10 journal articles focused on topics like HIV detection and pathogenesis.
Robert Pesich_PAVA_Stanford Resume v. 8_22_16Robert Pesich
Robert Pesich has extensive experience managing laboratory operations and research projects. He has overseen the daily activities of 25 researchers at Stanford University and the Palo Alto VA, including managing budgets, equipment, and regulatory compliance. Pesich has specialized skills in tissue sample processing, gene expression analysis, and bioinformatics. He has authored several publications characterizing gene expression profiles in normal and diseased tissues. Currently, Pesich also serves as President of a poetry non-profit organization.
This document provides a summary of Jack Ryan Reifert's education, experience, publications, and research skills. Reifert holds a Ph.D. in Molecular, Cellular, and Developmental Biology from UCSB and a B.S. in Pharmacological Chemistry from UCSD. He has postdoctoral and research experience studying mechanisms of amyloid beta induced neurodegeneration and ovarian cancer metastasis. Reifert has authored several publications investigating topics like tau fragmentation and the mechanism of action of cancer drug Bendamustine. His research skills include tissue culture, molecular techniques, microscopy, and cytotoxicity assays.
This document provides a summary of Jack Ryan Reifert's education, experience, publications, and research skills. Reifert holds a Ph.D. in Molecular, Cellular, and Developmental Biology from UCSB and a B.S. in Pharmacological Chemistry from UCSD. He has postdoctoral research experience at UCSB studying bacterial peptide display for ovarian cancer detection and inhibition. Reifert has over 10 years of experience in neuroscience research and industrial research, and he has authored several publications in peer-reviewed journals.
This research article describes a novel method using high-density peptide microarrays and computational analysis to identify B-cell epitopes in patients with celiac disease. Overlapping peptide sequences from native and deamidated gliadin proteins were synthesized onto silicon wafers. Serum samples from celiac patients and controls were tested on the microarrays. Computational analysis identified distinct epitope sets that differentiated celiac patients from controls with high accuracy. The identified epitopes have potential for developing improved diagnostic tests for celiac disease.
Jianying Xiao has over 16 years of experience in pharmaceutical research. She has expertise in in vivo and in vitro drug discovery techniques related to drug metabolism, infectious diseases, immunology, and cardio-metabolic disorders. She is proficient in various research techniques including animal handling, molecular biology, cell culture, and data analysis software. Jianying has worked at Merck & Co. for over 18 years, leading numerous projects that resulted in publications, patents, and awards. Her work has advanced drug programs from research through clinical trials.
This document summarizes research on immortalizing bovine pancreatic duct cells and making them tumorigenic through genetic manipulation. Key points:
1) Bovine pancreatic duct cells were immortalized by transfecting them with SV40 large T antigen cDNA. The immortalized cells retained features of normal duct cells.
2) The immortalized duct cells were then transfected with a mutated K-ras gene. This caused the cells to gain the ability to form tumors when injected into nude mice, suggesting K-ras plays an important role in pancreatic carcinogenesis.
3) The research aims to establish a model for the transition from normal duct cell to neoplastic cell in order to study the initial steps of pancreatic cancer development
Presentation from the 2014 Waterloo iGEM team at the Giant Jamboree in Boston. Read more about Staphylocide, our microbe engineered to silence antiobiotic resistance, on our 2014 wiki: http://2014.igem.org/Team:Waterloo.
This presentation is also available on the iGEM website: http://2014.igem.org/files/presentation/Waterloo_Championship.pdf
This document discusses a study examining the expression of cancer testis antigen (CTA) genes in glioma stem cells. The key findings are:
1) CTA genes were highly and frequently expressed in cancer stem cells isolated from glioma cell lines and tissues, compared to differentiated tumor cells.
2) Histone acetylation levels in promoter regions of CTA genes were high in cancer stem cells and low in differentiated cells, indicating epigenetic regulation of CTA gene expression.
3) CTA genes may be attractive targets for cancer vaccine therapy against glioma cancer stem cells due to their expression and ability to be presented as surface antigens on cancer stem cells.
Deborah P. Beebe is seeking a senior project manager position to utilize her 30 years of experience in biomedical research and project management. She has a PhD in Immunology and is a certified Project Management Professional (PMP). Her experience includes 9 years as Senior Project Manager at the National Cancer Institute and 15 years of progressive leadership roles at the National Heart, Lung, and Blood Institute including scientific review administrator, branch chief, and division director. She also has experience managing budgets, conducting research, and overseeing FDA regulatory processes for drug and biologic approvals.
Nicholas Grammatikakis is a Research Director at the Institute of Biosciences and Applications in Greece. He has over 30 years of experience in cellular and molecular biology research focusing on signal transduction in cancer and stress response. His research has studied mechanisms of kinase regulation, chemotherapeutic inhibition of oncogenic kinases, regulation of chaperone proteins and stress response, and identification of novel molecular chaperones. He has supervised many laboratory staff and students.
James J. Campbell has had an extensive career in immunology research. He is currently a Senior Director at ChemoCentryx, where he leads pre-clinical research teams investigating drug mechanisms of action. Previously, he was a Principal Investigator and Professor at Harvard Medical School, where he managed an immunology lab and published over 60 peer-reviewed papers. He has received many awards and honors for his research and expertise in T cell biology, chemokines, and skin and intestinal immunity.
R L Praveena has a Ph.D from the Indian Institute of Science Education and Research and expertise in cloning, protein purification, and the Wnt/Wg signaling pathway using fly models. She has strong backgrounds in molecular biology and cell biology and experience using various biotechnology tools and techniques. Her thesis involved screening for interacting partners of the genome organizer SATB1 and establishing its physical interaction with and regulation of the Wnt/Wg pathway through experiments including mammalian two-hybrid assays, reporter assays, pulldowns, and quantitative RT-PCR.
Lisa Salvador is a pharmaceutical research professional with over 10 years of industry experience focusing on clinical biomarkers, translational medicine, and drug development. She has a PhD in Cell Biology from Northwestern University and has held senior research roles at Bristol-Myers Squibb and GlaxoSmithKline. Her expertise includes cellular and molecular biology, gene therapy research, and diagnostic assay development. She has received numerous awards for her research contributions and leadership.
Maha Rizk has over 10 years of experience in biotechnology research, specializing in oncology biomarkers and drug development. She has expertise in assay development, cell culture, protein analysis, and data analysis. Currently she is a Senior Associate Scientist at Amgen developing assays to investigate biomarkers for cancer and inflammation.
This document discusses a study that investigated how inhibiting the epidermal growth factor receptor (EGFR) signaling pathway with the anti-EGFR monoclonal antibody IMC-C225 affects nuclear factor-kappa B (NF-κB) activation and regulation of apoptosis genes in human pancreatic cancer cells. The study found that IMC-C225 treatment blocked EGFR activation in pancreatic cancer cells, leading to decreased NF-κB DNA binding activity. This downregulation of NF-κB by IMC-C225 resulted in decreased expression of the anti-apoptotic genes bcl-xl and bfl-1. Therefore, targeting the NF-κB pathway with an anti-EGFR antibody may help restore apoptosis in pancreatic cancer cells and
1) Betacellulin (BTC) transgenic mice were studied to examine the effects of constitutive BTC overexpression on the urinary bladder.
2) BTC was detected in microvascular structures and umbrella cells of the uroepithelium lining. ERBB1 and ERBB4 receptors were also present in the uroepithelium.
3) BTC transgenic mice and mice that were double transgenic for BTC and a dominant kinase-dead EGFR mutant developed hyperplasia of the uroepithelium at 5 months of age, suggesting BTC signaling was not solely dependent on ERBB1/EGFR.
Jack Ryan Reifert has extensive experience in neuroscience research and industrial research. He received his Ph.D. in Molecular, Cellular, and Developmental Biology from UCSB, focusing on amyloid beta induced neurodegeneration and the involvement of tau. He has held postdoctoral positions at UCSB studying bacterial peptide display to inhibit ovarian cancer metastasis and at UCSD investigating wnt/β-catenin signaling in rheumatoid arthritis. Reifert has worked at several companies, contributing to FDA approval of Bendamustine and coordinating product releases. He is currently a postdoctoral fellow at UCSB studying targeted inhibition of proteases in ovarian cancer metastasis.
1. Professional Experience:
Senior Research Scientist, Neumedicines, Inc., Pasadena, CA 2008-2016
• Identified retained intron sequences in Interleukin 12A are common and may function in
immunobiology of IL-12
• Identified biomarkers associated with efficacy of IL-12 in treatment of CTCL (Cutaneous T cell
lymphoma) in Phase 2A clinical study
• Searched for biomarkers associated with radioprotection induced by IL-12
• Development of an enhanced tumor-directed huIL-12 protein
• Analysis of tumor cell lines for HSS1 (Hematopoietic Signal peptide-containing Secreted 1)
expression
Senior Associate Scientist, Cancer Biology, Amgen Inc., Thousand Oaks, CA 1996-2007
• Cloned, expressed, and characterized huTERT (human telomerase) from colon carcinoma cell line
• Validated telomerase as a potential cancer therapeutic target by demonstrating inhibition of
telomerase in epithelial carcinoma cells results in apoptosis
• Identified TP1 as a telomerase-associated protein
• Screened for small molecules inhibitors of the E3 ubiquitin ligase of the anaphase promoting
complex to develop as cancer therapeutics
• Screened for small molecule inhibitors of HIF (hypoxia inducible factor) to develop for cancer
therapy
Research Associate, Developmental Biology, Amgen Inc. 1993-1996
• Member of project team that first cloned and expressed thrombopoietin (MGDF)(megakaryocyte
growth and differentiation factor) by screening lambda and BAC human fetal liver libraries
1992 Daylight Ct.
Thousand Oaks, CA 91362
(805) 523-2435
VMar1@Yahoo.com
CURRICULUM VITAE
Vernon L. Mar
A Discovery Research Scientist with a background in molecular biology, cell biology, immunology, and
protein science. Extensive in-depth technical experience in laboratory research. Proven research success
in hematological growth factors, cancer biology, and vaccine development. Ready to take innovative
approaches to achieve research goals; e.g., development of a dramatically improved vaccine for major
childhood disease. Seeking opportunities in research and development to improve or develop new
treatments for human disease.
Summary:
2. • MCAT-1 (murine ecotropic retrovirus receptor) was identified as essential for normal
erythropoiesis by creating a germline null mutation of the gene
Research Associate, Molecular Biology and Vaccine Development, Amgen Inc. 1982-1993
• Developed a Pertussis toxoid with no enzymatic activity and retention of neutralizing epitope by
site mutagenesis of toxin gene (licensed to Novartis)
• Developed a methods for in vitro reassembly of Pertussis B oligomer from recombinant toxin
subunits
Staff Research Associate, Infectious Diseases Laboratory, VA Medical Center, Martinez, CA
• Identified a major source of cytomegalovirus reactivation in immunocompromised persons
(established that latent virus is reactivated in spleen B cell population and proliferation occurs in
permissive macrophages
Additional Experience:
Research Associate, Comparative Oncology Lab, Univ. of Calif. Davis
Graduate Teaching Assistant, Microbiology Dept., San Diego State Univ.
Laboratory Assistant, Dept. of Food Science and Technology, Univ. of Calif. Davis, Bodega Marine
Laboratory
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• Molecular Biology Procedures
- DNA purification and plasmid preps (classical methods-alkaline lysis, CsCl density centrifugation
or Qiagen silica resin methods)
- Molecular cloning (restriction digestion, DNA modification, cDNA, ligation, bacterial
transformation, site-directed mutagenesis and gene fusions)
- DNA analysis (agarose and acrylamide gel electrophoresis, Southern hybridization)
- Detection methods (radiolabeling of probes, colony or library screening (bacterial, phage, and BAC
libraries), β-Gal blue-white screening, GFP)
- PCR methods (PCR cloning and analysis, RT-PCR (Real Time), quantitative PCR (TaqMan)
analysis)
- RNA methods (isolation and purification, Northern blot analysis, microarray analysis (Rosetta
Resolver), in vitro transcription (Riboprobes), siRNA knock-down and RNAi expression vector)
- Software (MacVector, Vector NTI, Clone Manager)
• Protein Expression and Analysis
- SDS-PAGE ( Coomassie and silver staining, 35
S-Met-labeling, Western blot analysis)
- Bacterial and Mammalian expression (constitutive and inducible vectors (tet-inducible and
ecdysone systems)
- In vitro translation (rabbit reticulocyte lysate method)
- Expression reporter systems (luciferase, GFP, lac Z, beta-lactamase)
- Instrumentation (Tecan Safire, Rubystar, Wallac and Molecular Devices plate readers)
- Immunoprecipitation (protein A or G or Sepharose conjugate)
• Protein Purification
- Precipitation methods (ammonium sulfate, TCA, acetone methods)
- Column Purification methods (gel filtration (Sepharose), ion exhange (DEAE), affinity (antibody,
ligand-receptor)
- His-tagged protein purification (Ni-Sepharose)
- Ultra-filtration (centrifugal and stirred cell)
• Immunological Methods
- Immunohistochemical staining with fluorescent conjugated antibodies
- Flow cytometry with BD FACS Calibur (cell cycle analysis with propidium iodide stained cells and
fluorescent tagged antibodies)
- ELISA assays (with coated plates and bead assays, kinase assays with phospho-sp. antibodies)
- Alphalisa immunoassay
- Antibody arrays for biomarker development
Technological Experience:
3. 3
• Other Biochemical Assays
- Apoptosis assays (Annexin V, TUNEL, Caspase-Glo, PARP, M30)
- Cell proliferation assays (XTT, alamar blue, ATPlite)
- IGEN electrochemiluminescent (ECL) assay (ubiquitination assay)
• Cell Culture
- Primary cells and cell lines
- Tissue explants
- Virus plaque assays
- DNA transfections for transient and stable expression
• Animal experimentation (mice) (limited)
- IP, tail vein injections
- Spleenectomy
- Parotidectomy
• Computer Software
- Microsoft Office (Word, Excel, Power Point)
- GraphPad Prism
- Adobe Photoshop
Education:
M.S. in Microbiology, San Diego State University
Thesis title: "Structural Polypeptides of Ortho- and Paramyxoviruses"
Postgraduate courses, University of California, Davis
B.S. in Biochemistry and A.B. in Chemistry, University of California, Davis
4. Scientific Publications:
Jordan, M.C., V.L. Mar (1982) Spontaneous activation of latent cytomegalovirus from murine spleen
explants. Role of lymphocytes and macrophages in release and replication of virus. J. Clin. Invest. 70:
(4) 762-768.
Burnette, W.N., V.L. Mar, W. Cieplak, C.F. Morris, K.T. Kaljot, K.S. Marchitto, R.K. Sachdev, C.
Locht, and J.M. Keith (1988) Direct expression of Bordetella pertussis toxin subunits in Escherichia coli.
Bio/Technology 6:699-706.
Cieplak, W., W.N. Burnette, V.L. Mar, K. T. Kaljot, C.F. Morris, K.K. Chen, H. Sato, and J.M. Keith
(1988) Identification of a region in the Sl subunit of pertussis toxin that is required for enzymatic activity
and that contributes to the formation of a neutralizing antigenic determinant. Proc. Natl. Acad. Sci. USA
85:4667-4671.
Burnette, W.N., W. Cieplak, V.L. Mar, K. T. Kaljot, H. Sato, and J.M. Keith (1988) Pertussis toxin S1
mutant with reduced enzymatic activity and a conserved protective epitope. Science 242:72-74.
Burnette, W.N., V.L. Mar, W. Cieplak, C.F. Morris, K.T. Kaljot, H. Sato, and J.M. Keith (1988)
Toward development of a recombinant pertussis vaccine, p. 75-85. In L. Lasky (ed.), Technological
Advances in Vaccine Development. Alan R. Liss, Inc., New York.
Keith, J.M., W. Cieplak, V.L. Mar, K.T. Kaljot, K.S. Marchitto, H. Sato, and W.N. Burnette (1988)
Biochemical and immunological analyses of pertussis toxin subunits produced in recombinant Escherichia
coli, p. 99-103. In H. Ginsberg, F. Brown, R.A. Lerner, and R.M. Chanock (eds.), Vaccines 88. New
Chemical and Genetic Approaches to Vaccination: Prevention of AIDS and Other Viral, Bacterial, and
Parasitic Diseases. Cold Spring Harbor Laboratory, New York.
Burnette,W.N., D.W. Whiteley, V.L. Mar, D.L. Burns, H.R. Kaslow, W. Cieplak,J.M. Keith, and T.D.
Bartley (1989) Developments toward a recombinant pertussis vaccine. p. 1-7. In M.Z. Atassi (ed.),
Immunology of Proteins and Peptides, V: Vaccines: Mechanisms, Design, and Applications. Plenum
Publishing Corp., New York
Burnette, W.N., T.D. Bartley, D.W. Whiteley, V.L. Mar, W. Cieplak, J.M. Keith, and D.L. Bums
(1989) Recombinant analogs of pertussis toxin S1 subunit, p. 239-242. In R.A. Lerner, H. Ginsberg, R.M.
Chanock, and F. Brown (eds.), Vaccines 89. Modem Approaches to New Vaccines Including Prevention
of AIDS. Cold Spring Harbor Laboratory, New York.
Kaslow, H.R., J.D. Schlotterbeck, V.L. Mar, and W.N. Burnette (1988) Evidence that cysteine-41, but not
cysteine-200, decreases the ADP-ribosyltransferase activity of the S1 subunit of pertussis toxin. J. Biol.
Chem. 264:6386-6390.
Bartley, T.D., D.W. Whiteley, V.L. Mar, D.L. Burns and W.N. Burnette (1989) Pertussis holotoxoid
formed in vitro with a genetically deactivated S1 subunit. Proc. Natl. Acad. Sci. USA 86:8353-8357.
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5. Burnette, W.N., D. W. Whiteley, V.L. Mar, D.L. Bums, H.R. Kaslow, W. Cieplak, J.M. Keith, and
T.D. Bartley (1989) Developments toward a recombinant pertussis vaccine. Adv. Exp. Med. &
BioL251:1-7.
Cieplak, W., Jr., C. Locht, V.L. Mar, W.N. Bumette, and J.M. Keith. (1990) Photolabeling of authentic
and recombinant forms of the S1 subunit of pertussis toxin with NAD+. Biochem. J. 268:547-55 1.
Arciniega,J.L., R.D. Shahin, W.N. Bumette, T.D. Bartley, D.W. Whiteley, V.L. Mar, and D.L. Burns
(1991) Contribution of the B oligomer to the protective activity of inactivated pertussis toxin. Infection and
Immunity 59:3407-3410.
Burnette, W.N., V.L. Mar, V. W. Platler, J.D. Schlotterbeck, M.D. McGinley, D.S. Stoney, M.F. Rohde,
and H.R. Kaslow (1991) Site-specific mutagenesis of the catalytic subunit of cholera toxin: substituting lysine
for arginine 7 causes loss of activity. Infection and Immunity 59:4266-4270.
Burnette, W.N., V.L. Mar, T.D. Bartley, H.R. Kaslow, W. Cieplak, Jr., J.M. Keith, H. Sato, D.L. Bums,
J.L. Arciniega, R.D. Shahin, K. Redhead, K.H.G. Mills, K. Saukkonen, and E. Tuomanen. (1991) The
molecular engineering of pertussis toxoid. Dev. Biol. Standard. 73:75-78.
Saukkonen, K., W.N. Burnette, V.L. Mar, H.R. Masure, and E. Tuomanen (1992) Pertussis toxin has
eukaryotic-like carbohydrate recognition domains. Proc. Natl. Acad. Sci. USA 89:118-122.
Kaslow, H.R., J.D. Schlotterbeck, V.L. Mar, and W.N. Bumette (1992) Site-specific mutagenesis of the
pertussis toxin S1 subunit gene: effects of amino acid substitutions involving residues 50-58. Vaccine Res.
1:47-54.
Burnette, W.N., V.L. Mar, D.W. Whiteley, and T.D. Bartley (1992) Progress with a recombinant
whooping cough vaccine: a review. J. Royal Soc. of Med. 85:285-287.
Burnette, W.N., J.L. Arciniega, V.L. Mar, and D.L. Bums (1992) Properties of pertussis toxin B oligomer
assembled in vitro from recombinant polypeptides produced by Escherichia coli. Infection and Immunity
60:2252-2256.
Kaslow, H.R., B. Platler, T. Takada, J., Moss, V.L. Mar, and W.N. Burnette (1992) Effects of site-
directed mutagenesis on cholera toxin Al subunit ADP-ribosyltransferase activity. p.197-198. In Witholt et
al. (eds.), Bacterial Protein Toxins (Zbl. Bakt.Suppl. 23). Gustav Fisher, Stuttgart.
Van 'T Wout, J., W.N. Burnette, V.L. Mar, E. Rozdzinski, S.D. Wright, and E.I. Tuomanen (1992)
Role of carbohydrate recognition domains of pertussis toxin in adherence of Bordetella pertussis to human
macrophages. Infection and Immunity 60:3303-3308.
Rozdzinski, E., W.N. Burnette, T. Jones, V. Mar, and E. Tuomanen (1993) Prokaryotic peptides that block
leukocyte adherence to selectins. J. Exp. Med. 178:917-924.
Hase, C.C., L.S. Thai, M. Boesman-Finkelstein, V.L. Mar, W.N. Burnette, H.R. Kaslow, L.A. Stevens, J.
Moss, and R.A. Finkelstein (1994) Construction and characterization of recombinant Vibrio cholerae
strains producing inactive cholera toxin analogs. Infection and Immunity 62:3051-3057.
Burnette, W.N. and V.L. Mar (1994) Pertussis: molecular design, protein engineering, and assembly of a
hexameric toxoid vaccine, p. 149-164. In G. Pifat-Mrzljak (eds.) Supramolecular Structure and Function.
Balaban Publishers, Rehovoth, Israel.
Bartley, T.D., J. Bogenberger, P. Hunt et al. (1994) Identification and cloning of a megakaryocyte growth and
development factor that is a ligand for the cytokine receptor Mpl. Cell 77:1117-1124.
Chang, M.S., J. McNinch, R. Basu, J. Shutter, R. Hsu, C. Perkins, V. Mar et al. (1995) Cloning and
characterization of the human megakaryocyte growth and development factor (MGDF) gene. J. Biol. Chem.
270:511-514.
Perkins CP. Mar V. Shutter JR.del Castillo J. Danilenko DM. Medlock ES. Ponting IL. Graham M. Stark KL.
Zuo Y. Cunningham JM. Bosselman RA. Anemia and perinatal death result from loss of the murine ecotropic
retrovirus receptor in mCAT-L [Journal Article] Genes &Development. 11 (7): 914-25, 1997 Apr 1. UL
9106662
Harrington, L., McPhail, T., Mar, V., Zhou, W., Oulton, R., Amgen EST Program, Bass, M., Arruda, I., and M.
O. Robinson, A Mammalian Telomerase- Associated Protein, Science, 275, 973-977, 1997.
Harrington, L., Zhou, W., McPhail, T., Oulton, R., Yeung, D., Mar, V., Bass, M. and M. O. Robinson, The
human telomerase complex evolutionarily conserved catalytic and structural Subunits. Genes & Development.,
11,3109-3115,1997.
Zhang, X., Mar, V., Zhou, W., Harrington, L. and M. O. Robinson, Telomere shortening and apoptosis in
telomerase-inhibited human tumor cells, Genes & Development. 13,2388 -2399,1999.
Basile, Lena A., Ellefson, Dolph, Gallaher, Timothy K., Gluzman-Poltorak, Zoya, Junes-Gill, Katiana, Mar,
Vernon, Mendonca, Sarita, Miller, Joseph D., Tom, Jamie, Trinh, Alice (2012) HemaMaxTM
(rIL-12) is a
Potent Mitigator of Acute Radiation Injury in Mice and Non-Human Primates. PLOS 7(2): e30434.
Junes-Gill, Katiana, Lawrence, Chris E., Mar, Vernon, Shiri, Liron, and Basile, Lena A. (2014) Human
Hematopoietic Signal peptide-containing Secreted 1 (hHSS1) modulates genes and pathways in glioma:
implications for the regulation of tumorigenicity and angiogenesis. BMC Cancer. 14, 920-936.
Patent Application:
"Recombinant B oligomer of pertussis toxin", U.S. Patent Application #07/705,433, filed May 24, 1991.
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