Accomplished cancer biologist with focus on translational cancer research, metabolism and autophagy.

1. Shilpy Joshi, Ph.D
84 Cedar Lane, Apt-A, Highland Park, New Jersey 08904
(732) 289-7262, shilpy2010@hotmail.com; joshis1@cinj.rutgers.edu
Work Eligibility- United States Permanent Resident (Green Card)
Objective:
I am a driven, organized and innovative multidisciplinary cancer researcher who can rapidly understand complex
information in order to drive scientific results. I am a Cancer biologist by training with extensive expertise in
dealing of human patient samples, primary cells, cancer cell lines and mouse tumor models. I have broad
experience in running multiple collaborative projects to successful completion. Objective is to implement the
knowledge and the expertise for clinical cancer research and drug development.
Profile:
• 6+ years Postdoctoral experience
• Accomplished Cancer biologist with focus on translational cancer research, metabolism and autophagy
• Strong background in working with human cancer patient samples, primary cells, cancer cell lines, mouse
tumor models, 3D cultures
• Extensive experience in isotope tracer studies, Seahorse OCAR & ECAR and mitochondrial ETC enzymatic
assays
• Comprehensive training in CRISPR-Cas9(n), RNAi & shRNA (lentiviral) genome editing
• Preclinical drug efficacy studies in lung tumor mouse model
• Validation of targets for tumorigenesis/autophagy modulation
• Hybridoma, T cell response, cytokine profiling
• Presented research at scientific meetings, wrote grants, published scientific papers in peer reviewed journals
• Mentored junior scientists
Professional Experience:
Research Associate at Rutgers Cancer Institute of New Jersey (RCINJ), New Jersey, June 2015-present,
Mentor: Dr. Eileen White; resulting in an oral presentation award in NJCCR/RCINJ, an oral
presentation in Tristate Cancer Metabolism meeting, Princeton University, NJ
• Elucidation of metabolic differences between human renal oncocytoma vs normal kidney cells (in
collaboration with Joshua Rabinowitz lab, Princeton, NJ) : Ongoing project
• Effect of mitochondrial respiration defects on tumorigenesis by means of chemical inhibition
and/or Crispr KO of electron transport chain components : Ongoing project
NJCCR Postdoctoral fellow at RCINJ, New Jersey, May 2013-May 2015, Mentor: Dr. Eileen White;
resulting in travel grant award from International Keystone Symposia & a first author publication in
Cell Reports
• Genomic and biochemical analysis of human renal oncocytoma (in collaboration with MSKCC,
New York) : Completed project

2. Postdoctoral fellow at RCINJ, New Jersey, Feb 2011-May 2013, Mentor: Dr. Eileen White; resulting in
a 2 year NJCCR grant award of $100,000; Gallo award for scientific excellence by NJCCR/RCINJ ; first
author publication in Oncogene
• 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (PFKFB4) as a novel autophagy
regulator (in collaboration with Abraham lab, Pfizer and David Sabatini lab, MIT) :
Completed project
Education:
Ph.D. in Cell Biology and Immunology from Institute of Microbial Technology, Chandigarh, affiliate
of Jawaharlal Nehru University, New Delhi, India, 2003-2008
• Studies on cell envelope components of Mycobacteria: immunology of host pathogen interactions
Technical Skills:
• Models: Human patient samples, primary cell cultures, human/ mouse cancer cell lines, 3D- matrigel cultures,
Hybridoma, mouse tumor models.
• Cell biology: Seahorse (OCAR/ECAR), Flow cytometry, Incucyte, clonogenic, viability, cell based
reporter/functional assays, autophagy flux assays, drug treatment, Immunofluorescence assay.
• Molecular Biology: CRISPR-Cas9 mediated genome editing, RNAi lentiviral mediated stable knockdown of
targets, cloning, PCR and other general molecular biology techniques.
• Protein Biochemistry: Protein engineering, Purification, SDS PAGE, WB, IP, ChIP, ELISA, mitochondrial
enzymatic assays.
• Immunology: Hybridoma, T cell response, cytokine profiling
• Tissue analysis: Immunohistochemistry and Co-Immunostaining.
• Animal training: Adenocre nasal inhalation, oral gavage, subcutaneous, intraperitoneal injections, allograft
tumor assays, genotyping, pre-clinical drug efficacy studies in mice.
Professional skills:
§ Excellent analytical and problem-solving skills, team leader, innovative & persuasive.
§ Carried out independent/multi-institute collaborative projects to successful completion, mentored
junior scientists.
§ Well-organized in data collection, documentation (dossier/s) and good project management
strategies.
§ Good oral skills, presented research at national/international scientific meetings, wrote grants,
published scientific papers in peer- reviewed journals.
Most Recent peer-reviewed publications:
1. Kumar, N., Srivillibhuthur, M., Joshi, S., Walton, K. D., Zhou, A., Faller, W.J., Perekatt, A.O., Sansom, O. J.,
Gumucio, D.L., Xing, J., Bonder, E.M., Gao, N., White, E. and Verzi, M. (2016). A YY1-dependent increase in
aerobic metabolism is indispensable for intestinal organogenesis. Development. Manuscript accepted for
publication.
2. Joshi, S*., Tolkunov, D*., Aviv, H., Hakimi, A. A., Yao, M., Karsli-Uzunbas, G., Hsieh, J. J., Ganesan, S., Chan, C.
S., White, E (2015). The Genomic Landscape of Renal Oncocytoma Identifies a Metabolic Barrier to
Tumorigenesis. Cell Reports, Published online: November 19, 2015 (*Authors have equal contribution)
3. Strohecker, A.M*., Joshi, S*., Possemato, R., Abraham, R.T., Sabatini, D.M., White, E (2015). Identification of 6-
phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB4) as a Novel Autophagy Regulator by High
Content shRNA Screening. Oncogene. 2015 Nov 5; 34 (45): 5662-76.doi: 10.1038/onc.2015.23 Epub 2015 Ma16.
(*Authors have equal contribution)