Culture media in microbiology laboratory - topic deals with classification of culture media, types, their basic ingredients, role of each ingredients, method of preparation, sterilization and uses
Culture is the term for microorganisms grown in the lab, and medium provides nutrients for their growth. Culture media are used to isolate, study, and identify bacteria. Media are classified by consistency (liquid, solid, semisolid) and oxygen requirements. Different media, like nutrient agar or blood agar, support growth of various microbes. Selective and differential media help isolate specific bacteria. Culture media must provide appropriate nutrients, pH, and be sterilized before use to isolate bacteria and study their properties.
Culture media are used to support the growth of microorganisms. They can be liquid, semisolid, or solid and contain various nutrients like water, electrolytes, peptone, agar, meat extract, and blood or serum. Culture media are classified based on consistency and purpose of use. Common types include basal media, enriched media, selective media, differential media, transport media, and anaerobic and blood culture media. Automated systems now allow continuous monitoring of blood cultures for faster detection of microbial growth compared to conventional methods.
Culture media are used to grow microorganisms outside their natural environment in the laboratory. They provide nutrients to support microbial growth. Common media include nutrient broths and agar plates. Specialized media are sometimes needed to cultivate specific microbes. Media can be classified based on physical state (liquid, semisolid, solid), presence of oxygen, nutritional components (simple, complex, synthetic), and purpose (enrichment, selective, differential). Selective media inhibit all bacteria except the desired type, while differential media help distinguish between microbial species.
Culture media are the basic requirement to grow the microbes in the laboratory for various purpose like isolation, identification and research purposes.
Culture media in microbiology refers to the solid or liquid substances used to cultivate and grow microorganisms such as bacteria, fungi, and viruses in a laboratory setting. These media provide the necessary nutrients, environment, and conditions for microorganisms to thrive and reproduce. The choice of culture media depends on the specific requirements of the microorganisms being studied, as different organisms have varied nutritional needs and environmental preferences.
culture media
CULTURE – Is term given to microorganisms that are cultivated in the lab for the purpose of studying them.
MEDIUM – Is the term given to the combination of ingredients that will support the growth & cultivation of microorganisms outside their natural habitats.
Necessary Requirements for Growth of Bacteria
Distilled Water
Nitrogen containing compounds
Peptone- Golden granular powder
Complex mixture of partially digested protiens by proteolytic
enzymes pepsin, trysin or papain
Peptones, Proteoses, polypeptides, aminoacids, inorganic salts like phosphates
potassium & magnesium
Accessory growth factors like nicotinic acid & riboflavin
Energy sources
Suitable Ph- 7.2 – 7.4
Solidifying agents:
Gelatin– Protien
Agar— Chief component is Long chain Polysaccharide
Melts at 95°c & solidify only when cooled to about 42°c
1- 2% yields a suitable gel eg. Non-nutritive agar
According to Physical State:
Liquid – Peptone Water, Nutrient Broth
Semisolid – Nutrient Agar Stabs
Solid – Blood Agar
According to Oxygen requirement:
Aerobic Medium
Anaerobic Media
Culture Techniques and Maintenance of selected culturesMiracleLivinus1
This document provides information about microbial culture techniques and maintenance of selected cultures. It discusses what a microbial culture is and the history of culture media. It describes different types of culture media such as solid, liquid, and semi-solid media as well as specialized media like enriched, selective, and transport media. Specific media examples are given. Common culture methods like streak, pour plate, stab and liquid cultures are explained along with their purposes. Maintaining pure cultures and growing aerobic versus anaerobic organisms is also covered.
Culture is the term for microorganisms grown in the lab, and medium provides nutrients for their growth. Culture media are used to isolate, study, and identify bacteria. Media are classified by consistency (liquid, solid, semisolid) and oxygen requirements. Different media, like nutrient agar or blood agar, support growth of various microbes. Selective and differential media help isolate specific bacteria. Culture media must provide appropriate nutrients, pH, and be sterilized before use to isolate bacteria and study their properties.
Culture media are used to support the growth of microorganisms. They can be liquid, semisolid, or solid and contain various nutrients like water, electrolytes, peptone, agar, meat extract, and blood or serum. Culture media are classified based on consistency and purpose of use. Common types include basal media, enriched media, selective media, differential media, transport media, and anaerobic and blood culture media. Automated systems now allow continuous monitoring of blood cultures for faster detection of microbial growth compared to conventional methods.
Culture media are used to grow microorganisms outside their natural environment in the laboratory. They provide nutrients to support microbial growth. Common media include nutrient broths and agar plates. Specialized media are sometimes needed to cultivate specific microbes. Media can be classified based on physical state (liquid, semisolid, solid), presence of oxygen, nutritional components (simple, complex, synthetic), and purpose (enrichment, selective, differential). Selective media inhibit all bacteria except the desired type, while differential media help distinguish between microbial species.
Culture media are the basic requirement to grow the microbes in the laboratory for various purpose like isolation, identification and research purposes.
Culture media in microbiology refers to the solid or liquid substances used to cultivate and grow microorganisms such as bacteria, fungi, and viruses in a laboratory setting. These media provide the necessary nutrients, environment, and conditions for microorganisms to thrive and reproduce. The choice of culture media depends on the specific requirements of the microorganisms being studied, as different organisms have varied nutritional needs and environmental preferences.
culture media
CULTURE – Is term given to microorganisms that are cultivated in the lab for the purpose of studying them.
MEDIUM – Is the term given to the combination of ingredients that will support the growth & cultivation of microorganisms outside their natural habitats.
Necessary Requirements for Growth of Bacteria
Distilled Water
Nitrogen containing compounds
Peptone- Golden granular powder
Complex mixture of partially digested protiens by proteolytic
enzymes pepsin, trysin or papain
Peptones, Proteoses, polypeptides, aminoacids, inorganic salts like phosphates
potassium & magnesium
Accessory growth factors like nicotinic acid & riboflavin
Energy sources
Suitable Ph- 7.2 – 7.4
Solidifying agents:
Gelatin– Protien
Agar— Chief component is Long chain Polysaccharide
Melts at 95°c & solidify only when cooled to about 42°c
1- 2% yields a suitable gel eg. Non-nutritive agar
According to Physical State:
Liquid – Peptone Water, Nutrient Broth
Semisolid – Nutrient Agar Stabs
Solid – Blood Agar
According to Oxygen requirement:
Aerobic Medium
Anaerobic Media
Culture Techniques and Maintenance of selected culturesMiracleLivinus1
This document provides information about microbial culture techniques and maintenance of selected cultures. It discusses what a microbial culture is and the history of culture media. It describes different types of culture media such as solid, liquid, and semi-solid media as well as specialized media like enriched, selective, and transport media. Specific media examples are given. Common culture methods like streak, pour plate, stab and liquid cultures are explained along with their purposes. Maintaining pure cultures and growing aerobic versus anaerobic organisms is also covered.
- Definition
- Uses of culture media
- Basic composition of culture media
- Types of culture media
--Based on physical state
----solid medium
----semi solid medium
----liquid medium
--Based on ingredients
----Simple or basal medium.
----Complex medium.
----Synthetic or defined medium.
----Semisynthetic medium.
--Special medium
----Enriched media
----Enrichment media
----Selective media
----Differential media
----Indicator media
----Transport media
----Anaerobic media
-Media preparation
-Culture method
--Streak culture
--Lawn culture
-references
Here are some commonly used media for isolating the microorganisms:
Pseudomonas - King's B medium
Azospirillum - Ashby's mannitol agar
Azatobacter - Jensen's nitrogen-free medium
Rhizobium - Yeast extract mannitol agar
Escherichia coli - Eosin methylene blue agar, MacConkey agar
Fungi - Potato dextrose agar, Sabouraud dextrose agar
Yeast - Potato dextrose agar
Actinomycetes - Starch casein agar
This document provides information on bacterial culture medium and culture techniques. It discusses the history of bacterial culture, the need for culture methods, types of media including solid, liquid and selective media. Common media such as nutrient agar, blood agar and MacConkey agar are described. The document also covers culture methods like streak plate, pour plate and anaerobic culture techniques. Culture media provide a controlled environment for bacterial growth, aiding identification and characterization of bacteria.
This document discusses different types of culture media used to grow bacteria. It describes media as being solid, semisolid, or liquid based on consistency, and as simple, enriched, selective, indicator, differential, or composite based on function or properties. Key constituents of media like agar, peptone, yeast or meat extract, and NaCl are identified. Specific examples of enriched media (blood agar), enrichment broth (tetrathionate broth), selective media (MacConkey agar), indicator media (Wilson Blair media), and transport media (Stuart's transport media) are provided. The document conveys that different media types are used for isolating bacteria, demonstrating their properties, obtaining growth for tests, bacterial identification and typing
Culture media are used to grow bacteria for identification and study. They contain nutrients like carbon, nitrogen, water and growth factors. Common ingredients include meat extract and peptone. Media can be solid, liquid, or semi-solid depending on whether agar is added. Special media like selective, differential and enrichment media allow isolation of specific bacteria. Proper preparation, sterilization and storage of media is required for pure cultures.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on nutritional needs. Streaking is used to isolate pure cultures from clinical specimens. Pour plating quantifies viable bacteria while lawns are used for antibiotic testing. Transport media maintain delicate bacteria during specimen travel.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on ingredients. Streaking is used to isolate bacteria from clinical specimens onto solid media. Pour plating and liquid cultures are used for quantitative analysis and transporting samples respectively. Proper culture technique allows growth and study of bacteria.
This document discusses culture media and culture methods used to grow bacteria. It describes the various types of culture media including solid, liquid, and semi-solid media made with ingredients like agar. Different media are used for specific purposes such as enrichment, selection, and indication of bacterial properties. Culture methods like streaking, lawning, stabbing, and pouring are explained for isolating pure cultures of bacteria and studying their characteristics. Liquid cultures are also used for certain applications.
Culture media are substances used to grow microorganisms outside the body. They provide nutrients and an environment for microbes to grow. Culture media can be solid, semi-solid, or liquid, and are composed of ingredients like water, peptones, electrolytes, and agar or gelatin. Media are also classified based on their ingredients as simple, complex, synthetic, or special types. Special media include enriched, selective, differential, and transport media used for isolating specific microbes or preserving samples. Proper culture media allow microbes to be studied and identified to aid in diagnosis and treatment of infections.
This document provides an overview of different types of culture media used for growing microorganisms. It discusses the history and definitions of culture media. Media are classified based on consistency (liquid, semi-solid, solid) and method of growth detection (conventional vs automated). The document describes various types of media including simple/basal media, enriched media, enrichment media, selective media, differential media, transport media, and media for specific microorganisms. It provides examples of commonly used media and their purposes. The preparation, sterilization, testing and storage of media are also outlined.
Culture media are used to isolate bacteria from clinical specimens and perform biochemical tests to identify the causative agent. Culture media contain water, electrolytes, peptone as a nitrogen source, buffers to maintain pH, and indicators to detect acid or alkali production. Selective agents inhibit some bacteria. Agar is used to solidify media. Media can be liquid, semisolid, or solid. Routine media include basal, enriched, selective, differential, transport, and anaerobic media. Synthetic media use defined chemical components. Colony characteristics like size, shape, color, and hemolytic properties on blood agar help identify bacteria.
Culture media are used to isolate bacteria from clinical specimens and perform biochemical tests to identify the causative agent. Culture media contain water, electrolytes, peptone as a nitrogen source, buffers to maintain pH, and indicators to detect acid or alkali production. Selective agents inhibit some bacteria. Agar is used to solidify media. Media can be liquid, semisolid, or solid. Routine media include basal, enriched, selective, differential, transport, and anaerobic media. Synthetic media use defined chemical components. Colony characteristics like size, shape, color, and hemolytic properties on blood agar help identify bacteria.
This document summarizes culture methods used to grow bacteria. Culture media is used to isolate bacteria from infected material. Originally, Louis Pasteur used urine or meat broth as liquid media while Robert Koch used cooked cut potato as a solid medium. Agar was later introduced as a gelling agent for solid media. Various culture methods are described including streak, pour plate, and stab cultures. Biochemical tests like oxidase and indole are used to further identify bacteria grown on culture media.
Culture media are used to support the growth of microorganisms. They can be liquid, solid, or semisolid depending on their consistency. Culture media contain various nutrients like peptone, agar, and electrolytes. They are classified based on their oxygen requirement, nutrition type, and special characteristics. Examples of types of culture media include enriched, selective, differential, anaerobic, and blood culture media. Automated blood culture systems allow for continuous monitoring and faster detection of microbial growth compared to conventional methods.
Culture media are used to support the growth of microorganisms. There are different types of culture media based on consistency (liquid, solid, semisolid) and special characteristics (enriched, selective, differential). Common constituents include water, electrolytes, peptone, agar. Culture media can be classified based on consistency, oxygen requirement, nutrition level and special characteristics. Examples of specialized media include blood agar, chocolate agar, Lowenstein-Jensen medium and Thiosulfate Citrate Bile salts Sucrose agar. Automated systems like BacT/ALERT can rapidly detect microbial growth in blood cultures.
Culture is the term for microorganisms grown in the lab. A culture medium provides nutrients to support microbial growth. Specialized media are used for different purposes like enrichment, selection, or differentiation. Diagnostic cultures identify pathogens from samples and common tests include urine, stool, genital and skin samples. Culture techniques allow isolation and study of microbes and are essential tools in medical microbiology.
Culture media, or growth media, are mixtures of nutrients that support the growth of microorganisms. The basic requirements of culture media include an energy source, carbon source, nitrogen source, salts, satisfactory pH, oxidation reduction potential, and growth factors. Culture media can be classified based on consistency (solid, liquid, semisolid), constituents (simple, synthetic, complex, special), or whether they support aerobic or anaerobic growth. Solid media use agar to solidify and are used to isolate bacteria and study colony characteristics, while liquid media like broths allow for rapid growth but not isolation. Special media include enriched, enrichment, selective, indicator, differential, sugar, and transport media formulated for specific purposes.
This document discusses various tools and methods used for bacterial culture, including petri dishes, inoculation loops, autoclaves, incubators, agar, broth, laminar flow hoods, glass flasks, antibiotic discs, and anaerobic stations. It also describes different types of culture media such as solid media, semi-solid media, liquid media, basic media, enriched media, selective media, indicator media, and transport media. Key aspects of cultivating microorganisms like nutrients, pH, temperature, aeration, and salt concentration are also summarized.
Culture Media and culture technique.pptssuser957fe2
This document provides information on various culture media used to grow microorganisms. It discusses the early history of culture media beginning with Pasteur's use of liquid broths made from urine or meat extract. The importance of solid media for developing pure cultures is highlighted. Agar was later introduced as a solidifying agent since it does not melt at temperatures bacteria can grow at. Different types of media are described including solid, liquid, selective, differential, and enriched media. Composition and uses of several common media like nutrient agar, blood agar, MacConkey agar, Sabouraud dextrose agar, and Mueller Hinton agar are outlined.
Biological source method of preparation ,identification test uses of enzymesSUSHILBHOYAR2
This document provides information about 5 enzymes - diastase, papain, pepsin, trypsin, and pancreatin. It discusses the biological sources, methods of preparation, descriptions, identification tests, and uses of each enzyme. The key points covered are:
- Diastase is obtained from rat pancreas or fungi and is used as a digestant and to convert starch to sugars.
- Papain comes from papaya latex and is used as a meat tenderizer and anti-inflammatory.
- Pepsin is derived from pig stomach and used to treat gastric issues and in protein analysis.
- Trypsin is from ox pancreas and given to aid digestion or to remove dead tissue
- Definition
- Uses of culture media
- Basic composition of culture media
- Types of culture media
--Based on physical state
----solid medium
----semi solid medium
----liquid medium
--Based on ingredients
----Simple or basal medium.
----Complex medium.
----Synthetic or defined medium.
----Semisynthetic medium.
--Special medium
----Enriched media
----Enrichment media
----Selective media
----Differential media
----Indicator media
----Transport media
----Anaerobic media
-Media preparation
-Culture method
--Streak culture
--Lawn culture
-references
Here are some commonly used media for isolating the microorganisms:
Pseudomonas - King's B medium
Azospirillum - Ashby's mannitol agar
Azatobacter - Jensen's nitrogen-free medium
Rhizobium - Yeast extract mannitol agar
Escherichia coli - Eosin methylene blue agar, MacConkey agar
Fungi - Potato dextrose agar, Sabouraud dextrose agar
Yeast - Potato dextrose agar
Actinomycetes - Starch casein agar
This document provides information on bacterial culture medium and culture techniques. It discusses the history of bacterial culture, the need for culture methods, types of media including solid, liquid and selective media. Common media such as nutrient agar, blood agar and MacConkey agar are described. The document also covers culture methods like streak plate, pour plate and anaerobic culture techniques. Culture media provide a controlled environment for bacterial growth, aiding identification and characterization of bacteria.
This document discusses different types of culture media used to grow bacteria. It describes media as being solid, semisolid, or liquid based on consistency, and as simple, enriched, selective, indicator, differential, or composite based on function or properties. Key constituents of media like agar, peptone, yeast or meat extract, and NaCl are identified. Specific examples of enriched media (blood agar), enrichment broth (tetrathionate broth), selective media (MacConkey agar), indicator media (Wilson Blair media), and transport media (Stuart's transport media) are provided. The document conveys that different media types are used for isolating bacteria, demonstrating their properties, obtaining growth for tests, bacterial identification and typing
Culture media are used to grow bacteria for identification and study. They contain nutrients like carbon, nitrogen, water and growth factors. Common ingredients include meat extract and peptone. Media can be solid, liquid, or semi-solid depending on whether agar is added. Special media like selective, differential and enrichment media allow isolation of specific bacteria. Proper preparation, sterilization and storage of media is required for pure cultures.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on nutritional needs. Streaking is used to isolate pure cultures from clinical specimens. Pour plating quantifies viable bacteria while lawns are used for antibiotic testing. Transport media maintain delicate bacteria during specimen travel.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on ingredients. Streaking is used to isolate bacteria from clinical specimens onto solid media. Pour plating and liquid cultures are used for quantitative analysis and transporting samples respectively. Proper culture technique allows growth and study of bacteria.
This document discusses culture media and culture methods used to grow bacteria. It describes the various types of culture media including solid, liquid, and semi-solid media made with ingredients like agar. Different media are used for specific purposes such as enrichment, selection, and indication of bacterial properties. Culture methods like streaking, lawning, stabbing, and pouring are explained for isolating pure cultures of bacteria and studying their characteristics. Liquid cultures are also used for certain applications.
Culture media are substances used to grow microorganisms outside the body. They provide nutrients and an environment for microbes to grow. Culture media can be solid, semi-solid, or liquid, and are composed of ingredients like water, peptones, electrolytes, and agar or gelatin. Media are also classified based on their ingredients as simple, complex, synthetic, or special types. Special media include enriched, selective, differential, and transport media used for isolating specific microbes or preserving samples. Proper culture media allow microbes to be studied and identified to aid in diagnosis and treatment of infections.
This document provides an overview of different types of culture media used for growing microorganisms. It discusses the history and definitions of culture media. Media are classified based on consistency (liquid, semi-solid, solid) and method of growth detection (conventional vs automated). The document describes various types of media including simple/basal media, enriched media, enrichment media, selective media, differential media, transport media, and media for specific microorganisms. It provides examples of commonly used media and their purposes. The preparation, sterilization, testing and storage of media are also outlined.
Culture media are used to isolate bacteria from clinical specimens and perform biochemical tests to identify the causative agent. Culture media contain water, electrolytes, peptone as a nitrogen source, buffers to maintain pH, and indicators to detect acid or alkali production. Selective agents inhibit some bacteria. Agar is used to solidify media. Media can be liquid, semisolid, or solid. Routine media include basal, enriched, selective, differential, transport, and anaerobic media. Synthetic media use defined chemical components. Colony characteristics like size, shape, color, and hemolytic properties on blood agar help identify bacteria.
Culture media are used to isolate bacteria from clinical specimens and perform biochemical tests to identify the causative agent. Culture media contain water, electrolytes, peptone as a nitrogen source, buffers to maintain pH, and indicators to detect acid or alkali production. Selective agents inhibit some bacteria. Agar is used to solidify media. Media can be liquid, semisolid, or solid. Routine media include basal, enriched, selective, differential, transport, and anaerobic media. Synthetic media use defined chemical components. Colony characteristics like size, shape, color, and hemolytic properties on blood agar help identify bacteria.
This document summarizes culture methods used to grow bacteria. Culture media is used to isolate bacteria from infected material. Originally, Louis Pasteur used urine or meat broth as liquid media while Robert Koch used cooked cut potato as a solid medium. Agar was later introduced as a gelling agent for solid media. Various culture methods are described including streak, pour plate, and stab cultures. Biochemical tests like oxidase and indole are used to further identify bacteria grown on culture media.
Culture media are used to support the growth of microorganisms. They can be liquid, solid, or semisolid depending on their consistency. Culture media contain various nutrients like peptone, agar, and electrolytes. They are classified based on their oxygen requirement, nutrition type, and special characteristics. Examples of types of culture media include enriched, selective, differential, anaerobic, and blood culture media. Automated blood culture systems allow for continuous monitoring and faster detection of microbial growth compared to conventional methods.
Culture media are used to support the growth of microorganisms. There are different types of culture media based on consistency (liquid, solid, semisolid) and special characteristics (enriched, selective, differential). Common constituents include water, electrolytes, peptone, agar. Culture media can be classified based on consistency, oxygen requirement, nutrition level and special characteristics. Examples of specialized media include blood agar, chocolate agar, Lowenstein-Jensen medium and Thiosulfate Citrate Bile salts Sucrose agar. Automated systems like BacT/ALERT can rapidly detect microbial growth in blood cultures.
Culture is the term for microorganisms grown in the lab. A culture medium provides nutrients to support microbial growth. Specialized media are used for different purposes like enrichment, selection, or differentiation. Diagnostic cultures identify pathogens from samples and common tests include urine, stool, genital and skin samples. Culture techniques allow isolation and study of microbes and are essential tools in medical microbiology.
Culture media, or growth media, are mixtures of nutrients that support the growth of microorganisms. The basic requirements of culture media include an energy source, carbon source, nitrogen source, salts, satisfactory pH, oxidation reduction potential, and growth factors. Culture media can be classified based on consistency (solid, liquid, semisolid), constituents (simple, synthetic, complex, special), or whether they support aerobic or anaerobic growth. Solid media use agar to solidify and are used to isolate bacteria and study colony characteristics, while liquid media like broths allow for rapid growth but not isolation. Special media include enriched, enrichment, selective, indicator, differential, sugar, and transport media formulated for specific purposes.
This document discusses various tools and methods used for bacterial culture, including petri dishes, inoculation loops, autoclaves, incubators, agar, broth, laminar flow hoods, glass flasks, antibiotic discs, and anaerobic stations. It also describes different types of culture media such as solid media, semi-solid media, liquid media, basic media, enriched media, selective media, indicator media, and transport media. Key aspects of cultivating microorganisms like nutrients, pH, temperature, aeration, and salt concentration are also summarized.
Culture Media and culture technique.pptssuser957fe2
This document provides information on various culture media used to grow microorganisms. It discusses the early history of culture media beginning with Pasteur's use of liquid broths made from urine or meat extract. The importance of solid media for developing pure cultures is highlighted. Agar was later introduced as a solidifying agent since it does not melt at temperatures bacteria can grow at. Different types of media are described including solid, liquid, selective, differential, and enriched media. Composition and uses of several common media like nutrient agar, blood agar, MacConkey agar, Sabouraud dextrose agar, and Mueller Hinton agar are outlined.
Biological source method of preparation ,identification test uses of enzymesSUSHILBHOYAR2
This document provides information about 5 enzymes - diastase, papain, pepsin, trypsin, and pancreatin. It discusses the biological sources, methods of preparation, descriptions, identification tests, and uses of each enzyme. The key points covered are:
- Diastase is obtained from rat pancreas or fungi and is used as a digestant and to convert starch to sugars.
- Papain comes from papaya latex and is used as a meat tenderizer and anti-inflammatory.
- Pepsin is derived from pig stomach and used to treat gastric issues and in protein analysis.
- Trypsin is from ox pancreas and given to aid digestion or to remove dead tissue
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
TEST BANK For Community Health Nursing A Canadian Perspective, 5th Edition by...Donc Test
TEST BANK For Community Health Nursing A Canadian Perspective, 5th Edition by Stamler, Verified Chapters 1 - 33, Complete Newest Version Community Health Nursing A Canadian Perspective, 5th Edition by Stamler, Verified Chapters 1 - 33, Complete Newest Version Community Health Nursing A Canadian Perspective, 5th Edition by Stamler Community Health Nursing A Canadian Perspective, 5th Edition TEST BANK by Stamler Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Pdf Chapters Download Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Pdf Download Stuvia Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Study Guide Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Ebook Download Stuvia Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Questions and Answers Quizlet Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Studocu Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Quizlet Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Pdf Chapters Download Community Health Nursing A Canadian Perspective, 5th Edition Pdf Download Course Hero Community Health Nursing A Canadian Perspective, 5th Edition Answers Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Ebook Download Course hero Community Health Nursing A Canadian Perspective, 5th Edition Questions and Answers Community Health Nursing A Canadian Perspective, 5th Edition Studocu Community Health Nursing A Canadian Perspective, 5th Edition Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Pdf Chapters Download Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Pdf Download Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Study Guide Questions and Answers Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Ebook Download Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Questions Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Studocu Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Stuvia
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Identification and nursing management of congenital malformations .pptx
culture media.ppt
1. Dr. Atit Shah, M.D.
Associate Professor,
Department of Microbiology
N.H.L. Medical College
2. Able to classify
Know basic constituents & their role
Should give examples
Various sterilization techniques used for
culture media preparation
3. Are substances which are used to grow
bacteria in laboratory
Difficult to identify by morphology alone
4. Original culture media
used by Louis Pasteur was
liquid medium – Meat
broth
Disadvantages :
◦ Bacteria doesn't exhibit specific characteristics
◦ Difficult to isolate different bacteria in mixed
population
5. Cooked cut potato - The first solid medium
used by Robert Koch
Gelatin
◦ Unsatisfactory – melts at 24 c
◦ Also liquefied by many proteolytic bacteria
Agar – Frau Hesse
Petridish – Richard Petri
6. Universally used solidifying agent
Obtained from sea-weeds
Made up of polysaccharide, also contains
inorganic salts & protein like material
No nutritive value, not affected by growth
Melts at 98 c and usually sets at 42 c
Available as powder or in long shreds
7. Universal ingredient of all culture media
Complex mixture of partially digested protein
◦ Constituents : proteoses, polypeptides & amino-acids
Also contains
◦ inorganic salts – PO4, K, Mg
◦ Certain accessory growth factors –riboflavin
Special brands
◦ neopeptone, vegpeptone, meat extract – lab lemco
8. Nutrients
- Energy source
- Carbon source
- Nitrogen source
Mineral salts
◦ Sulphates, phosphates, chlorides & carbonates of
K, Mg & Ca.
A suitable pH – 7.2 – 7.4
Accessory growth factors
- Tryptophan for Salmonella typhi
- X & V factors for H. influenzae
9. Based on the consistency:
Liquid Peptone water, Nutrient broth
Semisolid Nutrient agar stabs
Solid Blood agar, Serum agar
Based on Oxygen requirement:
- Aerobic media
- Anaerobic media
10. Simple media
Complex media
- Enriched media
- Differential media
- Enrichment media
- Selective media
- Transport media
11. Liquid media
- 1 %Peptone water
1g peptone + 0.5gNacl + 100 ml water
- Nutrient broth
peptone water + 1% meat extract
Solid media
- Nutrient agar (nutrient broth + 2% Agar)
Use: To grow non-fastidious microorganisms
Consists of only basic necessities
14. Basal media + Blood/serum/egg
Used to grow bacteria which are more
exacting in their nutritional needs
Examples
◦ Blood agar
◦ Chocolate agar
◦ Loeffler’s serum slope
◦ Dorset’s egg medium
15. Nutrient agar + 5 to 10% sheep blood
Cool the sterile nutrient agar to 450 c
Add the blood aseptically with constant
shaking
Mix the blood with molten nutrient agar
Pour in to the Petri dishes & allow to set
Use: To cultivate the fastidious organisms
Pneumococci, streptococci
19. Also called as - Heated blood agar
Heat nutrient agar to about 750 c
Add blood to the molten nutrient agar and
allow to remain at 750c
Gently mix till it is chocolate brown in color
Pour in Petri dishes/ test tubes for slopes
Use: To culture H. influenzae & N.meningitidis
20.
21.
22. N. broth 100ml+Serum300ml+1gmGlucose
Method of preparation :
◦ Glucose broth – sterilize by steaming
◦ Add serum aseptically
◦ Distribute in small screw-capped bottles
◦ Sterilize by inspissations, at 82oc for 1hr x 3 days
Use: To cultivate C. diphtheriae
23. Glucose broth + coagulated hens egg
Sterilize by inspissation
Use : to grow C.diphtheriae, M.tuberculosis
24. When certain substances are added to a liquid
medium which enhance the growth of the
pathogenic organisms and suppress the
unwanted bacteria
- Selenite-F broth – Salmonella typhi
- Alkaline peptone water – Vibrio cholerae
Use
To prevent the non-pathogenic or commensal bacteria
from overgrowing the pathogenic bacteria
25. 1 % Alkaline peptone water
Selenite F/Tetrathoionate
broth
26. Serve the same purpose as Enrichment media but
are solid in consistency
- Wilson & Blair’s medium
- S.S. agar
- Lowenstein Jensen’s medium
- T.C.B.S. agar
Use: To cultivate
Salmonella & Shigella
Mycobacteria
Vibrio
28. Mineral salt solution - 600ml
Malachite green solution - 20ml
Beaten egg - 1000ml
Mix the above
Distribute in Mc Cartney bottles
Sterilize by Inspissation
Use: To cultivate Mycobacteria
32. Contains :
◦ N.agar
◦ Bile salts
◦ Lactose
◦ Neutral red
Bacteria fermenting lactose produce acid and
this changes the color of the indicator and the
colonies turn pink
Use: To differentiate
Lactose fermenters (E. coli, Klebsiella)
Non-lactose fermenters (Salmonella,Shigella)
33.
34.
35. Are used in case of delicate organisms
whenever there is a delay in the
transportation of the specimen to the lab
To maintain viability of them & to prevent
the multiplication of non-pathogenic
bacteria
- Stuart’s medium - Gonococci
- Cary-Blair’s medium - V. cholerae
- V-R medium - V. cholerae
40. Contains :
◦ Peptone infusion broth
◦ Meat particles of fresh bullock heart
Use :
◦ To culture anaerobic organism
41.
42. Dextrose - 4 gm%
Neopeptone - 1 gm%
Agar - 1.5 gm%
Distilled water - 100 ml
pH - 5.4
Use: For the cultivation of Fungi
43.
44.
45. Peptone water – 100 ml, Desired sugar 1
gm% and Andrade's indicator – 0.005%
soln(1ml)
Dissolve the desired carbohydrate in
peptone water and steam for 30 min or
sterilize by filtration.
Distribute into sterile test tube containing
inverted Durham’s tubes to detect gas
production and steam for 30 min
Use: To test the fermenting ability of an
organism
46.
47. Autoclave - all media
Tyndallisation
◦ Heat-labile substances like sugar solutions
Inspissation
◦ Protein containing media
◦ Egg or serum containing media
48. Mackie & Mc Cartney
Practical Medical Microbiology, 13th ed
Text Book of Microbiology
Ananthanarayan & Paniker, 7th edition
Standard Operative procedure manual for
Microbiology laboratories.
Ministry of Health & Family welfare, Govt. of
India