This document provides information on culture media and methods used for growing and identifying bacteria. It discusses the various types of culture media including solid, liquid, and semi-solid media as well as specialized media like selective, differential and indicator media. Specific media like blood agar, MacConkey agar and triple sugar iron agar are described. Culture methods like streak plating, pour plating, and stab and stroke cultures are outlined. The document also covers biochemical tests performed using culture media, such as oxidase, indole, citrate, and urease tests to identify bacterial properties. Finally, techniques for culturing anaerobic bacteria in an oxygen-free environment are summarized.
This document provides information on various culture media and methods used to culture and identify bacteria. It discusses:
1) The need to culture bacteria separately on solid or liquid media to obtain pure cultures that can be studied. Common media include nutrient agar, blood agar, and broths.
2) Types of media such as solid, liquid, enriched, selective, differential and special media formulated for specific purposes.
3) Culture methods like streaking, lawning, stabbing and pour plating used to isolate bacteria and study their properties.
4) Requirements for culturing anaerobic bacteria in specialized conditions with low oxygen levels.
5) Biochemical tests that provide additional information for bacterial identification
This document discusses culture media and culture methods used to grow bacteria. It describes the various types of culture media including solid, liquid, and semi-solid media made with ingredients like agar. Different media are used for specific purposes such as enrichment, selection, and indication of bacterial properties. Culture methods like streaking, lawning, stabbing, and pouring are explained for isolating pure cultures of bacteria and studying their characteristics. Liquid cultures are also used for certain applications.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on nutritional needs. Streaking is used to isolate pure cultures from clinical specimens. Pour plating quantifies viable bacteria while lawns are used for antibiotic testing. Transport media maintain delicate bacteria during specimen travel.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on ingredients. Streaking is used to isolate bacteria from clinical specimens onto solid media. Pour plating and liquid cultures are used for quantitative analysis and transporting samples respectively. Proper culture technique allows growth and study of bacteria.
This document summarizes culture methods used to grow bacteria. Culture media is used to isolate bacteria from infected material. Originally, Louis Pasteur used urine or meat broth as liquid media while Robert Koch used cooked cut potato as a solid medium. Agar was later introduced as a gelling agent for solid media. Various culture methods are described including streak, pour plate, and stab cultures. Biochemical tests like oxidase and indole are used to further identify bacteria grown on culture media.
Culture Techniques and Maintenance of selected culturesMiracleLivinus1
This document provides information about microbial culture techniques and maintenance of selected cultures. It discusses what a microbial culture is and the history of culture media. It describes different types of culture media such as solid, liquid, and semi-solid media as well as specialized media like enriched, selective, and transport media. Specific media examples are given. Common culture methods like streak, pour plate, stab and liquid cultures are explained along with their purposes. Maintaining pure cultures and growing aerobic versus anaerobic organisms is also covered.
This document discusses various types of culture media used to grow bacteria. It describes simple and special media, including enrichment, selective, indicator, differential, and transport media. Examples are given for each type of medium and their uses are explained, such as isolating pathogens or distinguishing bacterial properties. Common media and tests are also summarized, like MacConkey agar, triple sugar iron agar, and the indole test.
- Definition
- Uses of culture media
- Basic composition of culture media
- Types of culture media
--Based on physical state
----solid medium
----semi solid medium
----liquid medium
--Based on ingredients
----Simple or basal medium.
----Complex medium.
----Synthetic or defined medium.
----Semisynthetic medium.
--Special medium
----Enriched media
----Enrichment media
----Selective media
----Differential media
----Indicator media
----Transport media
----Anaerobic media
-Media preparation
-Culture method
--Streak culture
--Lawn culture
-references
This document provides information on various culture media and methods used to culture and identify bacteria. It discusses:
1) The need to culture bacteria separately on solid or liquid media to obtain pure cultures that can be studied. Common media include nutrient agar, blood agar, and broths.
2) Types of media such as solid, liquid, enriched, selective, differential and special media formulated for specific purposes.
3) Culture methods like streaking, lawning, stabbing and pour plating used to isolate bacteria and study their properties.
4) Requirements for culturing anaerobic bacteria in specialized conditions with low oxygen levels.
5) Biochemical tests that provide additional information for bacterial identification
This document discusses culture media and culture methods used to grow bacteria. It describes the various types of culture media including solid, liquid, and semi-solid media made with ingredients like agar. Different media are used for specific purposes such as enrichment, selection, and indication of bacterial properties. Culture methods like streaking, lawning, stabbing, and pouring are explained for isolating pure cultures of bacteria and studying their characteristics. Liquid cultures are also used for certain applications.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on nutritional needs. Streaking is used to isolate pure cultures from clinical specimens. Pour plating quantifies viable bacteria while lawns are used for antibiotic testing. Transport media maintain delicate bacteria during specimen travel.
Bacteria must be cultured to study their properties and identify them. Different culture media and methods are used depending on the purpose. Agar is commonly used to make solid media for growing isolated colonies. Media can be simple, complex, or selective depending on ingredients. Streaking is used to isolate bacteria from clinical specimens onto solid media. Pour plating and liquid cultures are used for quantitative analysis and transporting samples respectively. Proper culture technique allows growth and study of bacteria.
This document summarizes culture methods used to grow bacteria. Culture media is used to isolate bacteria from infected material. Originally, Louis Pasteur used urine or meat broth as liquid media while Robert Koch used cooked cut potato as a solid medium. Agar was later introduced as a gelling agent for solid media. Various culture methods are described including streak, pour plate, and stab cultures. Biochemical tests like oxidase and indole are used to further identify bacteria grown on culture media.
Culture Techniques and Maintenance of selected culturesMiracleLivinus1
This document provides information about microbial culture techniques and maintenance of selected cultures. It discusses what a microbial culture is and the history of culture media. It describes different types of culture media such as solid, liquid, and semi-solid media as well as specialized media like enriched, selective, and transport media. Specific media examples are given. Common culture methods like streak, pour plate, stab and liquid cultures are explained along with their purposes. Maintaining pure cultures and growing aerobic versus anaerobic organisms is also covered.
This document discusses various types of culture media used to grow bacteria. It describes simple and special media, including enrichment, selective, indicator, differential, and transport media. Examples are given for each type of medium and their uses are explained, such as isolating pathogens or distinguishing bacterial properties. Common media and tests are also summarized, like MacConkey agar, triple sugar iron agar, and the indole test.
- Definition
- Uses of culture media
- Basic composition of culture media
- Types of culture media
--Based on physical state
----solid medium
----semi solid medium
----liquid medium
--Based on ingredients
----Simple or basal medium.
----Complex medium.
----Synthetic or defined medium.
----Semisynthetic medium.
--Special medium
----Enriched media
----Enrichment media
----Selective media
----Differential media
----Indicator media
----Transport media
----Anaerobic media
-Media preparation
-Culture method
--Streak culture
--Lawn culture
-references
This document provides an overview of culture media and culture methods used in microbiology. It discusses the different types of culture media based on consistency (solid, liquid, semi-solid), ingredients (simple, complex, synthetic), and oxygen requirements (aerobic, anaerobic). Common media like nutrient agar, blood agar and selective media are described. The preparation of culture media and methods like streak plating, pour plating, and stab culturing are explained. The aim is to learn about culture media, demonstrate preparation techniques, and different culturing methods for isolating and growing bacteria.
A culture media is a special medium used in microbiological laboratories to grow different kinds of microorganisms. A growth or a culture medium is composed of different nutrients that are essential for microbial growth.
Since there are many types of microorganisms, each having unique properties and requiring specific nutrients for growth, there are many types based on what nutrients they contain and what function they play in the growth of microorganisms.
A culture may be solid or liquid. The solid culture media is composed of a brown jelly like substance known as agar. Different nutrients and chemicals are added to it to allow the growth of different microorganisms.
Culture media are used to grow microorganisms outside the body. They consist of water, energy sources, carbon sources, nitrogen sources, minerals, and sometimes special growth factors. Culture methods include solid, liquid, and semi-solid media. Common techniques are streak plating to isolate colonies and pour plating for viable counts. Special media like enriched, selective, differential and indicator media are used to isolate specific microbes or detect certain properties. Proper culture methods and conditions are needed to grow different microorganisms.
This document discusses various conventional methods used for microbiological examination of foods, including culture media and methods, microbial staining techniques, dye reduction tests, and biochemical tests. It describes different types of culture media such as simple/basal media, complex media, synthetic/defined media, and special media. It also explains various culture methods, staining techniques like Gram staining and acid-fast staining, motility testing, and biochemical tests including catalase, coagulase, oxidase, and urease tests.
B.sc. (micro) i em unit 4.4 culturing & isolatingRai University
The document discusses culturing and isolating bacteria. Bacteria must be grown (cultured) separately (isolated) on culture media to obtain pure cultures for study. Various culture media are used, including solid, liquid, and semi-solid media made with ingredients like agar, nutrients, and dyes. Differential and selective media contain substances that allow bacteria to be distinguished based on properties like sugar fermentation and toxin production. Streak plating is used to isolate single colonies from a mixed culture to obtain a pure isolate.
CULTURE METHODS
Indications for culture -
Isolate bacteria in pure cultures.
Demonstrate their properties.
Obtain sufficient growth for preparation of antigens & for other tests.
Typing bacterial isolates.
Antibiotic sensitivity.
Estimate viable counts.
Maintain stock cultures.
Streak culture or surface plating
Lawn or carpet culture
Stroke culture
Stab culture
Pour plate method
Anaerobic methods of culturing bacteria
Streak Culture
Routinely employed for isolation
Platinum / Nichrome loops
LAWN OR CARPET CULTURE
Uniform surface growth
Bacteriophage typing
Antibiotic sensitivity testing
Preparation of bacterial antigens & vaccines
STOKE CULTURE
Tubes containing agar slopes
For slide agglutination & other diagnostic tests.
STAB CULTURE
By puncturing a suitable medium with a long, straight charged wire.
For gelatin liquefaction, stock cultures & motility
POUR PLATE METHOD
1 ml of appropriately diluted inoculum is added to 15 ml of molten agar and poured on petridish.
Colonies appear through out the depth of medium.
Used to estimate viable count, recommended method for quantitative urine cultures.
BROTH CULTURE
Inoculated by a charged loop, pipette or syringes.
For blood cultures & sterility testing.
This document discusses culture media and culture methods. It provides information on the major contributors to culture media, including Louis Pasteur, Robert Koch, and Frau Hesse. It describes the basic requirements of culture media such as nutrients, mineral salts, and pH. Different types of culture media are outlined, including solid, liquid, and semi-solid media as well as enriched, selective, indicator, and differential media. Common media like blood agar and MacConkey agar are also mentioned. The document concludes by noting that culture methods depend on the intended purpose, such as isolating bacteria in pure culture or demonstrating their properties.
Culture media are substances used to grow microorganisms outside the body. They provide nutrients and an environment for microbes to grow. Culture media can be solid, semi-solid, or liquid, and are composed of ingredients like water, peptones, electrolytes, and agar or gelatin. Media are also classified based on their ingredients as simple, complex, synthetic, or special types. Special media include enriched, selective, differential, and transport media used for isolating specific microbes or preserving samples. Proper culture media allow microbes to be studied and identified to aid in diagnosis and treatment of infections.
Culture media and Cultivation of Bacteria DR.C.P.PRINCEDR.PRINCE C P
Purpose of culturing are
Isolation of bacteria ( pure culture)
Diagnosis of infectious diseases
Properties of bacteria i.e. culturing bacteria is the initial step in studying its morphology and its identification.
Maintenance of stock cultures.
Estimate viable counts. Water , air, milk testing
To test for antibiotic sensitivity.
To create antigens for laboratory use.
Vaccine preparation
Sterility testing
Preparation of pharmaceutical products like antibiotics, enzymes, toxins etc
Certain genetic studies and manipulations of the cells also need that bacteria to be cultured in vitro.
Culturing on solid media is another convenient way of separating bacteria in mixture.
An artificial culture media must provide similar environmental and nutritional conditions that exist in the natural habitat of a bacterium.
A culture medium contains water, a source of carbon & energy, source of nitrogen, trace elements and some growth factors.
PPT prepared by:
DR.C. P. PRINCE
HOD & Associate Professor
Department of Microbiology
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
This document discusses various culture methods used for bacteria, including their purposes and procedures. Streak culture is used to isolate bacteria in pure culture and obtain separated colonies. Lawn culture provides uniform bacterial growth for tests like phage typing. Stroke and stab cultures provide pure bacterial growth for diagnostic tests. Pour plate culture allows estimating bacterial counts and is used for urine cultures. Liquid cultures are used for tests like blood cultures and continuous culture. Anaerobic culture methods aim to reduce oxygen levels using techniques like vacuum production, gas displacement, chemical or biological oxygen absorption, or reducing agents in media.
Culture refers to microorganisms grown in the lab, and medium refers to the nutrients that support microbial growth. The food materials required to grow microbes outside the body is called culture medium. Different types of media include solid, semisolid, liquid, aerobic, anaerobic, simple, complex, enriched, selective, differential, and indicator media. Specialized media are used for microbial enrichment, selection, differentiation, and transport. Culture media allow isolation and study of microbes through supporting their growth.
This document discusses culture media and culture methods. It begins by defining culture and medium, and then provides a brief history of the development of culture media. The document goes on to describe the various types of culture media, including classifications based on consistency, constituents, oxygen requirements, and purpose. It also discusses methods for identifying bacteria, such as staining, colony morphology, biochemical tests like catalase, and assessing motility. The overall purpose is to provide an overview of the different types of culture media and methods used to culture and identify microorganisms in a laboratory setting.
This document discusses culture media used to grow microorganisms in microbiology laboratories. It defines culture media as a special medium that provides nutrients for microbial growth. It then classifies culture media into three types based on physical state: solid, semisolid, and liquid broth media. It further classifies media based on ingredients into simple/basal media, undefined/complex media, and defined/synthetic media. The document also discusses several special types of media and their uses, as well as common culture methods like streak plating and lawn culture. It concludes by describing the McIntosh and Fildes jar method for obtaining anaerobic conditions for culturing anaerobic bacteria.
Culture media are used to grow bacteria in pure cultures for study. Solid media, like agar, allow bacteria to form distinct colonies that can be easily identified and isolated. Liquid media are used for inoculum preparation and some diagnostic tests. Different types of media, including enriched, selective, and indicator media, are used to isolate or identify specific bacteria based on their nutritional and biochemical properties. A variety of culture methods, such as streak plating, pour plating and liquid cultures, are employed for different purposes like isolating pure cultures, antibiotic testing, and maintaining stock cultures.
This document provides information on various types of culture media used for growing microorganisms in the laboratory. It discusses liquid, solid and semisolid media, and differentiates between simple, complex, enriched, selective, differential, indicator and transport media. Specific examples of commonly used media are provided for each type, along with their compositions and uses. The document aims to outline the basic requirements, classifications and characteristics of different culture media.
1. Culture methods are used to isolate bacteria in pure culture, demonstrate their properties, obtain sufficient growth for tests, and maintain stock cultures.
2. Common culture methods include streak culture, lawn culture, stroke culture, stab culture, pour plate culture, and liquid culture.
3. Special methods like anaerobic culture techniques are needed to isolate and grow anaerobic bacteria in the absence of oxygen using methods that generate hydrogen and carbon dioxide gases.
Assessing Reflexes of children in paediatrics pptxchriskimeu103
This document discusses several important newborn reflexes that help ensure infant survival. Newborn reflexes originate in the central nervous system and are present at birth but typically disappear during development as voluntary movements emerge. Common reflexes examined include sucking, rooting, palmar grasp, plantar grasp, Moro, tonic neck, and Babinski reflexes. Clinicians check for normal reflexes in newborns to assess neurological functioning, as abnormal or missing reflexes could indicate issues.
community health volunteer notes (1).pptxchriskimeu103
The document defines a community health volunteer in Kenya and outlines their selection, training, duties, remuneration, and the community health services they support. A community health volunteer must be a Kenyan citizen over 18, literate, a resident of the area for at least 5 years, and willing to serve without pay. They are selected at community meetings and undergo basic and technical training. Their duties include providing health education, treating minor ailments, record keeping, and referring more serious cases. Counties are responsible for remunerating and motivating volunteers.
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This document provides an overview of culture media and culture methods used in microbiology. It discusses the different types of culture media based on consistency (solid, liquid, semi-solid), ingredients (simple, complex, synthetic), and oxygen requirements (aerobic, anaerobic). Common media like nutrient agar, blood agar and selective media are described. The preparation of culture media and methods like streak plating, pour plating, and stab culturing are explained. The aim is to learn about culture media, demonstrate preparation techniques, and different culturing methods for isolating and growing bacteria.
A culture media is a special medium used in microbiological laboratories to grow different kinds of microorganisms. A growth or a culture medium is composed of different nutrients that are essential for microbial growth.
Since there are many types of microorganisms, each having unique properties and requiring specific nutrients for growth, there are many types based on what nutrients they contain and what function they play in the growth of microorganisms.
A culture may be solid or liquid. The solid culture media is composed of a brown jelly like substance known as agar. Different nutrients and chemicals are added to it to allow the growth of different microorganisms.
Culture media are used to grow microorganisms outside the body. They consist of water, energy sources, carbon sources, nitrogen sources, minerals, and sometimes special growth factors. Culture methods include solid, liquid, and semi-solid media. Common techniques are streak plating to isolate colonies and pour plating for viable counts. Special media like enriched, selective, differential and indicator media are used to isolate specific microbes or detect certain properties. Proper culture methods and conditions are needed to grow different microorganisms.
This document discusses various conventional methods used for microbiological examination of foods, including culture media and methods, microbial staining techniques, dye reduction tests, and biochemical tests. It describes different types of culture media such as simple/basal media, complex media, synthetic/defined media, and special media. It also explains various culture methods, staining techniques like Gram staining and acid-fast staining, motility testing, and biochemical tests including catalase, coagulase, oxidase, and urease tests.
B.sc. (micro) i em unit 4.4 culturing & isolatingRai University
The document discusses culturing and isolating bacteria. Bacteria must be grown (cultured) separately (isolated) on culture media to obtain pure cultures for study. Various culture media are used, including solid, liquid, and semi-solid media made with ingredients like agar, nutrients, and dyes. Differential and selective media contain substances that allow bacteria to be distinguished based on properties like sugar fermentation and toxin production. Streak plating is used to isolate single colonies from a mixed culture to obtain a pure isolate.
CULTURE METHODS
Indications for culture -
Isolate bacteria in pure cultures.
Demonstrate their properties.
Obtain sufficient growth for preparation of antigens & for other tests.
Typing bacterial isolates.
Antibiotic sensitivity.
Estimate viable counts.
Maintain stock cultures.
Streak culture or surface plating
Lawn or carpet culture
Stroke culture
Stab culture
Pour plate method
Anaerobic methods of culturing bacteria
Streak Culture
Routinely employed for isolation
Platinum / Nichrome loops
LAWN OR CARPET CULTURE
Uniform surface growth
Bacteriophage typing
Antibiotic sensitivity testing
Preparation of bacterial antigens & vaccines
STOKE CULTURE
Tubes containing agar slopes
For slide agglutination & other diagnostic tests.
STAB CULTURE
By puncturing a suitable medium with a long, straight charged wire.
For gelatin liquefaction, stock cultures & motility
POUR PLATE METHOD
1 ml of appropriately diluted inoculum is added to 15 ml of molten agar and poured on petridish.
Colonies appear through out the depth of medium.
Used to estimate viable count, recommended method for quantitative urine cultures.
BROTH CULTURE
Inoculated by a charged loop, pipette or syringes.
For blood cultures & sterility testing.
This document discusses culture media and culture methods. It provides information on the major contributors to culture media, including Louis Pasteur, Robert Koch, and Frau Hesse. It describes the basic requirements of culture media such as nutrients, mineral salts, and pH. Different types of culture media are outlined, including solid, liquid, and semi-solid media as well as enriched, selective, indicator, and differential media. Common media like blood agar and MacConkey agar are also mentioned. The document concludes by noting that culture methods depend on the intended purpose, such as isolating bacteria in pure culture or demonstrating their properties.
Culture media are substances used to grow microorganisms outside the body. They provide nutrients and an environment for microbes to grow. Culture media can be solid, semi-solid, or liquid, and are composed of ingredients like water, peptones, electrolytes, and agar or gelatin. Media are also classified based on their ingredients as simple, complex, synthetic, or special types. Special media include enriched, selective, differential, and transport media used for isolating specific microbes or preserving samples. Proper culture media allow microbes to be studied and identified to aid in diagnosis and treatment of infections.
Culture media and Cultivation of Bacteria DR.C.P.PRINCEDR.PRINCE C P
Purpose of culturing are
Isolation of bacteria ( pure culture)
Diagnosis of infectious diseases
Properties of bacteria i.e. culturing bacteria is the initial step in studying its morphology and its identification.
Maintenance of stock cultures.
Estimate viable counts. Water , air, milk testing
To test for antibiotic sensitivity.
To create antigens for laboratory use.
Vaccine preparation
Sterility testing
Preparation of pharmaceutical products like antibiotics, enzymes, toxins etc
Certain genetic studies and manipulations of the cells also need that bacteria to be cultured in vitro.
Culturing on solid media is another convenient way of separating bacteria in mixture.
An artificial culture media must provide similar environmental and nutritional conditions that exist in the natural habitat of a bacterium.
A culture medium contains water, a source of carbon & energy, source of nitrogen, trace elements and some growth factors.
PPT prepared by:
DR.C. P. PRINCE
HOD & Associate Professor
Department of Microbiology
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
This document discusses various culture methods used for bacteria, including their purposes and procedures. Streak culture is used to isolate bacteria in pure culture and obtain separated colonies. Lawn culture provides uniform bacterial growth for tests like phage typing. Stroke and stab cultures provide pure bacterial growth for diagnostic tests. Pour plate culture allows estimating bacterial counts and is used for urine cultures. Liquid cultures are used for tests like blood cultures and continuous culture. Anaerobic culture methods aim to reduce oxygen levels using techniques like vacuum production, gas displacement, chemical or biological oxygen absorption, or reducing agents in media.
Culture refers to microorganisms grown in the lab, and medium refers to the nutrients that support microbial growth. The food materials required to grow microbes outside the body is called culture medium. Different types of media include solid, semisolid, liquid, aerobic, anaerobic, simple, complex, enriched, selective, differential, and indicator media. Specialized media are used for microbial enrichment, selection, differentiation, and transport. Culture media allow isolation and study of microbes through supporting their growth.
This document discusses culture media and culture methods. It begins by defining culture and medium, and then provides a brief history of the development of culture media. The document goes on to describe the various types of culture media, including classifications based on consistency, constituents, oxygen requirements, and purpose. It also discusses methods for identifying bacteria, such as staining, colony morphology, biochemical tests like catalase, and assessing motility. The overall purpose is to provide an overview of the different types of culture media and methods used to culture and identify microorganisms in a laboratory setting.
This document discusses culture media used to grow microorganisms in microbiology laboratories. It defines culture media as a special medium that provides nutrients for microbial growth. It then classifies culture media into three types based on physical state: solid, semisolid, and liquid broth media. It further classifies media based on ingredients into simple/basal media, undefined/complex media, and defined/synthetic media. The document also discusses several special types of media and their uses, as well as common culture methods like streak plating and lawn culture. It concludes by describing the McIntosh and Fildes jar method for obtaining anaerobic conditions for culturing anaerobic bacteria.
Culture media are used to grow bacteria in pure cultures for study. Solid media, like agar, allow bacteria to form distinct colonies that can be easily identified and isolated. Liquid media are used for inoculum preparation and some diagnostic tests. Different types of media, including enriched, selective, and indicator media, are used to isolate or identify specific bacteria based on their nutritional and biochemical properties. A variety of culture methods, such as streak plating, pour plating and liquid cultures, are employed for different purposes like isolating pure cultures, antibiotic testing, and maintaining stock cultures.
This document provides information on various types of culture media used for growing microorganisms in the laboratory. It discusses liquid, solid and semisolid media, and differentiates between simple, complex, enriched, selective, differential, indicator and transport media. Specific examples of commonly used media are provided for each type, along with their compositions and uses. The document aims to outline the basic requirements, classifications and characteristics of different culture media.
1. Culture methods are used to isolate bacteria in pure culture, demonstrate their properties, obtain sufficient growth for tests, and maintain stock cultures.
2. Common culture methods include streak culture, lawn culture, stroke culture, stab culture, pour plate culture, and liquid culture.
3. Special methods like anaerobic culture techniques are needed to isolate and grow anaerobic bacteria in the absence of oxygen using methods that generate hydrogen and carbon dioxide gases.
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This document discusses several important newborn reflexes that help ensure infant survival. Newborn reflexes originate in the central nervous system and are present at birth but typically disappear during development as voluntary movements emerge. Common reflexes examined include sucking, rooting, palmar grasp, plantar grasp, Moro, tonic neck, and Babinski reflexes. Clinicians check for normal reflexes in newborns to assess neurological functioning, as abnormal or missing reflexes could indicate issues.
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A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
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2. Bacteria have to be grown (cultured) for
them to be identified.
By appropriate procedures they have to be
grown separately (isolated) on culture
media and obtained as pure for study.
History
The original media used by Louis Pasteur
– urine or meat broth
Liquid medium – diffuse growth
Solid medium – discrete colonies.
3. Colony – macroscopically visible collection of
millions of bacteria originating from a
single bacterial cell.
Cooked cut potato by Robert Koch –
earliest solid medium
Gelatin – not satisfactory
- liquefy at 24oC
4. Agar
Used for preparing solid medium
Obtained from seaweeds.
No nutritive value
Not affected by the growth of the bacteria.
Melts at 98oC & sets at 42oC
2% agar is employed in solid medium
5. Types of culture media
I. Based on their consistency
a) solid medium
b) liquid medium
c) semi solid medium
II. Based on the constituents/ ingredients
a) simple medium
b) complex medium
c) synthetic or defined medium
d) Special media
6. Special media
– Enriched media
– Enrichment media
– Selective media
– Indicator media
– Differential media
– Sugar media
– Transport media
– Media for biochemical reactions
III.Based on Oxygen requirement
- Aerobic media
- Anaerobic media
7. Solid media – contains 2% agar
Colony morphology, pigmentation, hemolysis can
be appreciated.
Eg: Nutrient agar, Blood agar
Liquid media – no agar.
For inoculum preparation, Blood culture, for the
isolation of pathogens from a mixture.
Eg: Nutrient broth
Semi solid medium – 0.5% agar.
Eg: Motility medium
8.
9. Simple media / basal media
- Eg: NB, NA
- NB consists of peptone, meat extract,
NaCl,
- NB + 2% agar = Nutrient agar
10. Complex media
Media other than basal media.
They have added ingredients.
Provide special nutrients
Synthetic or defined media
Media prepared from pure chemical
substances and its exact composition is
known
Eg: peptone water – 1% peptone + 0.5%
NaCl in water
11. Enriched media
Substances like blood, serum, egg are
added to the basal medium.
Used to grow bacteria that are exacting in
their nutritional needs.
Eg: Blood agar, Chocolate agar
13. Enrichment media
Liquid media used to isolate
pathogens from a mixed
culture.
Media is incorporated with
inhibitory substances to
suppress the unwanted
organism.
Eg:
– Selenite F Broth – for the
isolation of Salmonella, Shigella
– Alkaline Peptone Water – for
Vibrio cholerae
14. Selective media
The inhibitory substance is added to a solid
media.
Eg:
Mac Conkey’s medium for gram negative
bacteria
TCBS – for V.cholerae
LJ medium – M.tuberculosis
Wilson and Blair medium – S.typhi
Potassium tellurite medium – Diphtheria
bacilli
17. Indicator media
These media contain an indicator which
changes its colour when a bacterium
grows in them.
Eg:
– Blood agar
– Mac Conkey’s medium
– Christensen’s urease medium
20. Differential media
A media which has substances
incorporated in it enabling it to distinguish
between bacteria.
Eg: Mac Conkey’s medium
– Peptone
– Lactose
– Agar
– Neutral red
– Taurocholate
Distinguish between lactose fermenters &
non lactose fermenters.
22. Sugar media
Media containing any fermentable
substance.
Eg: glucose, arabinose, lactose, starch
etc.
Media consists of 1% of the sugar in
peptone water.
Contain a small tube (Durham’s tube) for
the detection of gas by the bacteria.
23.
24. Transport media
Media used for transporting the
samples.
Delicate organisms may not
survive the time taken for
transporting the specimen
without a transport media.
Eg:
– Stuart’s medium – non nutrient
soft agar gel containing a
reducing agent
– Buffered glycerol saline – enteric
bacilli
25. Anaerobic media
These media are used to grow anaerobic
organisms.
Eg: Robertson’s cooked meat medium,
Thioglycolate medium.
26. BIOCHEMICAL TEST & REACTIONS
They provide additional information for the
identification of the bacterium.
The tests include:
– Oxidase test
– Triple sugar iron agar (TSI)
– Indole test
– Citrate utilization
– Urease test
27. OXIDASE TEST
Detects the presence of an enzyme “oxidase”
produced by certain bacteria which will
reduce the dye – tetramethyl-p-phenylene
diamine dihydrochloride.
Positive test is indicated by the development
of a purple colour.
Oxidase positive – Pseudomonas, Vibrio,
Neisseriae
Oxidase negative – Salmonella, Shigella
28.
29. TRIPLE SUGAR IRON AGAR (TSI)
It is a composite media used to study different
properties of a bacterium – sugar fermentation, gas
production and H2S production.
In addition to peptone, yeast extract & agar, it
contains 3 sugars – Glucose, Lactose, Sucrose.
The Iron salt – Ferric citrate indicates H2S
production.
Phenol red is the indicator.
It is an orange red medium with a slant and a butt.
pH of the medium – 7.4
30. TSI REACTIONS:
Yellow – Acid
Pink - Alkaline
Yellow slant / Yellow butt (A/A) – Lactose fermenters.
Pink slant / Yellow butt (K/A) – Non lactose
fermenters.
Pink slant / no colour change (K/K) – Non fermenters
Black colour – H2S production.
Gas bubbles or crack in the medium – gas
production.
LF – E.coli, Klebsiella
NLF – Salmonella, Shigella
H2S - Proteus
31.
32. INDOLE TEST
Used to detect indole production by the organism.
They produce indole from tryptophan present in
peptone water.
After overnight incubation, a few drops of indole
reagent (Kovac’s reagent) is added.
Positive test is indicated by a pink ring.
– Positive indole test – pink ring
– Negative indole test - yellow ring
Indole positive – E.coli
Indole negative – Klebsiella, Salmonella.
33.
34. CITRATE UTILIZATION
Done in Simmon’s Citrate medium.
To detect the ability of certain bacteria to utilize
citrate as the sole source of carbon.
Contains Sodium citrate and bromothymol blue as
the indicator.
If citrate is utilized, alkali is produced which turns
the medium to blue.
– Citrate positive – blue colour
– Citrate negative – green colour
Positive – Klebsiella
Negative – E.coli
35.
36. UREASE TEST
Done in Christensen’s urease medium.
This test is used to detect organisms that
produce urease.
Urease produced by the organisms split
urea into ammonia and CO2.
– Urease positive – pink colour
– Urease negative – yellow colour
Positive – Proteus, Klebsiella
Negative – E.coli, Salmonella
37.
38. CULTURE METHODS
Culture methods employed depend on the purpose
for which they are intended.
The indications for culture are:
– To isolate bacteria in pure cultures.
– To demonstrate their properties.
– To obtain sufficient growth for the preparation of
antigens and for other tests.
– For bacteriophage & bacteriocin susceptibility.
– To determine sensitivity to antibiotics.
– To estimate viable counts.
– Maintain stock cultures.
40. STREAK CULTURE
Used for the isolation of bacteria in pure culture
from clinical specimens.
Platinum wire or Nichrome wire is used.
One loopful of the specimen is transferred onto
the surface of a well dried plate.
Spread over a small area at the periphery.
The inoculum is then distributed thinly over the
plate by streaking it with a loop in a series of
parallel lines in different segments of the plate.
On incubation, separated colonies are obtained
over the last series of streaks.
41.
42.
43. LAWN CULTURE
Provides a uniform surface growth of the
bacterium.
Uses
– For bacteriophage typing.
– Antibiotic sensitivity testing.
– In the preparation of bacterial antigens and
vaccines.
Lawn cultures are prepared by flooding the
surface of the plate with a liquid suspension of
the bacterium.
45. STROKE CULTURE
Stroke culture is made in
tubes containing agar slope /
slant.
Uses
–Provide a pure growth of
bacterium for slide
agglutination and other
diagnostic tests.
46. STAB CULTURE
Prepared by puncturing a suitable medium
– gelatin or glucose agar with a long,
straight, charged wire.
Uses
–Demonstration of gelatin liquefaction.
–Oxygen requirements of the bacterium
under study.
–Maintenance of stoke cultures.
48. POUR PLATE CULTURE
Agar medium is melted (15 ml) and cooled to
45oC.
1 ml of the inoculum is added to the molten agar.
Mix well and pour to a sterile petri dish.
Allow it to set.
Incubate at 37oC, colonies will be distributed
throughout the depth of the medium.
Uses
– Gives an estimate of the viable bacterial count in a
suspension.
– For the quantitative urine cultures.
49. LIQUID CULTURES
Liquid cultures are inoculated by touching with a
charged loop or by adding the inoculum with
pipettes or syringes.
Uses
– Blood culture
– Sterility tests
– Continuous culture methods
Disadvantage
– It does not provide a pure culture from mixed
inocula.
51. ANAEROBIC CULTURE METHODS
Anaerobic bacteria differ in their requirement
and sensitivity to oxygen.
Cl.tetani is a strict anaerobe – grows at an
oxygen tension < 2 mm Hg.
Methods:
– Production of vacuum
– Displacement of oxygen with other gases
– Chemical method
– Biological method
– Reduction of medium
52. Production of vacuum:
Incubate the cultures in a vacuum
desiccator.
Displacement of oxygen with other gases
Displacement of oxygen with hydrogen,
nitrogen, helium or CO2.
Eg: Candle jar
53.
54. Chemical method
Alkaline pyrogallol absorbs oxygen.
McIntosh – Fildes’ anaerobic jar
Consists of a metal jar or glass jar with a metal
lid which can be clamped air tight.
The lid has 2 tubes – gas inlet and gas outlet
The lid has two terminals – connected to
electrical supply.
Under the lid – small grooved porcelain spool,
wrapped with a layer of palladinised asbestos.
55.
56. Working:
Inoculated plates are placed inside the jar and
the lid clamped air tight.
The outlet tube is connected to a vacuum pump
and the air inside is evacuated.
The outlet tap is then closed and the inlet tube is
connected to a hydrogen supply.
After the jar is filled with hydrogen, the electric
terminals are connected to a current supply, so
that the palladinised asbestos is heated.
Act as a catalyst for the combination of hydrogen
with residual oxygen.
57. Gaspak
Commercially available disposable
envelope.
Contains chemicals which generate H2 and
CO2 on addition of water.
Cold catalyst – in the envelope
Indicator is used – reduced methylene blue.
– Colourless – anaerobically
– Blue colour – on exposure to oxygen
58.
59. Biological method
Absorption of oxygen by incubation with
aerobic bacteria, germinating seeds or
chopped vegetables.
Reduction of oxygen
By using reducing agents – 1% glucose,
0.1% Thioglycolate