The document outlines a project investigating the cultivation and biochemical properties of non-native fungi species in Mexico, focusing on their biotechnological potential for industrial and food applications. Selected species include shiitake, oyster mushrooms, and reishi, with objectives to optimize growth conditions and evaluate their nutritional and health benefits. The project aims to enhance knowledge and open new cultivation opportunities while considering ethical and legal aspects of introducing non-native species.

1. CULTIVATION AND
CHARACTERIZATION OF
NON-NATIVE FUNGI IN
MEXICO
Frida Martinez Maximiano

2. General
Objective
To investigate the growth, optimal
cultivation conditions, and biochemical
properties of non-native fungal
species in Mexico, in order to evaluate
their biotechnological potential for
industrial and food applications.
Selected Species
1. Lentinula edodes (Shiitake)
2. Pleurotus ostreatus (Oyster
Mushroom)
3. Ganoderma lucidum (Reishi)

3. L I T E R AT U R E
R E V I E W
Research existing
literature on the selected
species, focusing on their
cultivation requirements,
nutritional properties,
applications, and health
benefits.

4. A C Q U I S I T I O N
O F C U L T U R E S
Obtain strains of the
selected species
through specialized
mushroom culture
suppliers or microbial
banks. Ensure that the
strains are viable and
of high quality.

5. O P T I M I Z AT I O N
O F
C U L T I VAT I O N
C O N D I T I O N S
Establish experiments
to determine the
optimal cultivation
conditions
(temperature,
humidity, substrate,
pH, etc.) for each
species.

6. B I O C H E M I C A L
C H A R A C T E R I Z
AT I O N
Analyze the biochemical
properties of the cultivated
fungi. This
includes:Nutritional
composition (proteins,
carbohydrates, lipids,
vitamins, minerals).
•Antioxidant activity.
•Antimicrobial potential.

7. E V A L U AT I O N
O F
B I O T E C H N O L
O G I C A L
A P P L I C AT I O N S
Investigar posibles
aplicaciones de los
hongos cultivados en
áreas como la
alimentación, la medicina,
y la biorremediación.

8. Expected Impact
This project will not only
contribute to the knowledge about
non-native fungal species in
Mexico but could also open new
opportunities for their cultivation
and use in various industries,
thereby improving sustainability
and biodiversity in the country.

9. Final
Considerations
It is important to consider the
ethical and legal aspects
related to the introduction of
non-native species, ensuring
that the project complies with
biosafety and environmental
conservation regulations.