This portfolio documents Brooke Dale's coursework in crime scene investigation from Spring 2016. It includes documentation and analysis of various forensic science techniques practiced in the class like: protecting crime scenes, entomology, hair follicle analysis, fingerprinting, shoe impressions, blood droplet testing, bloodstain measurements, evidence collection kits, sexual assault evidence collection and testing. Photos show the students practicing these techniques on samples like dismembered pigs, their own fingerprints, blood droplets and stains, and simulated sexual assault evidence.

1. CRIME SCENE
INVESTIGATION PORTFOLIO
BROOKE DALE
SPRING 2016

2. PROTECTING THE CRIME SCENE INTEGRITY
 Considerations of a crime scene:
Indoors, Outdoors, Ingress, Egress,
transient items
 Protective Equipment: Disposable
gloves, Masks, Hair Nets, Shoe
Boots
 Photography, Videography, Notes
and Sketches
 Chain of Custody
 Admitting to Mistakes

3. ENTOMOLOGY
• What is Entomology?
• Entomology in the forensic disciple is a broad study of how insects
interrelate specifically with the decomposition and disintegration
of a human body.

4. ENTOMOLOGY CONTINUED
This is a pig that was dismembered and put into
a bin to start decomposing without being
directly exposed to the elements.
This is a photo of a pig that was just laying on
the ground; directly exposed to the elements;
decomposition has started as you can see the
first set of larva has taken place.

5. HAIR FOLLICLES
Below is where we looked at the different stages of
hair growth; Anagen, Catagen, and Telogen.
We applied what we learned about hair samples and looked
at them under a microscope. We didn’t look at just human
hair, either.

6. FINGERPRINTING
Here we took our own fingerprints and put
them on a fingerprinting sheet, we also put our
fingerprints on a balloon and blew it up to see
our prints.
This is a photo of a print I lifted off of a pair of
sunglasses using back fingerprinting powder on
a semi-dark surface.

7. FINGERPRTING
CONTINUED
This is myself, in action, dusting for
prints.

8. SHOE IMPRESSIONS/CASTING
This is where we made our boot impression in
some soil. And we mixed our casting agent with
water to put on top of our impression.
After some time had passed and the casting dried, we
dug out the impression, cleaned it up and came out
with a really good sample. You will notice the
emblems/ logos are visible from boot-to-casting.

9. BLOOD DROPLET TESTING/ANALYSIS
In this class we were doing testing for blood stains on
a tile using Leucomalachite Green (LMG), Filter
papers, and Hydrogen Peroxide reagents.
This is an in-action picture of testing the blood droplets.
Here is where we used the Leucomalachite Green on our
swabs to test for the presence of Blood.

10. BLOODSTAIN MEASUREMENTS
Here is an exercise where I learned how to
measure bloodstains. Which is Width (W)
divided by Length (L) equals the Ratio| then you
take Sin-1 and that equals your Angle of Impact.
This was an exercise where we learned how to
determine an Area of Convergence; did this by taking
the tail of the blood droplets and tracing them down
and finding where the majority connect.

11. COLLECTION KITS
We have gone in and observed what was in a
blood/urine collection kit, and the proper
procedures to handling the items and going over
the chain of command.
This is what was inside of our kit; we had blood
vials/syringes and a cup for the urine, safety
seals and instructions.

12. SEXUAL ASSAULT
Items in a sexual assault kit:
• known blood/ saliva samples
• blood/urine collection
• vaginal/anal/& oral swabs & smear
slides
• additional swabs for dried body fluids/
bitemarks/ licks/kisses
• materials for collecting fingernail
samples
• pubic hair combing
• debris collection
• proper packaging for clothes
• documentation.

13. SEXUAL ASSAULT PRESUMPTIVE TESTS
• We had two assignments:
• The first one we took an alternate light source to locate spots on our cloth to which we don’t know if it
is seminal fluid or another liquid. We took a sharpie and circled some viable spots to test further. After
leaving the dark room, we then prepared to test pieces of the cloth for seminal fluid using the Acid
Phosphatase (AP) Test which is a presumptive test.
• The second one we took already collected swabs (oral and vaginal) and then did a P30 confirmatory test
for sperm.

14. SEXUAL ASSAULT ANALYSIS (CLOTH)

15. SEXUAL ASSAULT ANALYSIS (SWABS)

16. FIN