The document provides guidance on the regional application process for Global Fund funding during the 2014-2016 allocation period. It outlines that regional applications will follow a two-step process, beginning with an Expression of Interest. Only eligible and strategically focused Expressions of Interest will be invited to submit a full concept note. There will be two calls for Expressions of Interest in 2014 and 2015. Successful applicants will then submit concept notes according to established deadlines, which will be reviewed by a Technical Review Panel. Eligibility requirements for regional applicants and the countries included are also described. The overall process aims to support strategic regional investments that address issues like cross-border interventions.