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Bovine
          Ovary – primary
           reproductive
           organ, produces
           gametes and sex
           hormones
          Oviduct –
           transport sperm
           to ovum and
           embryo to uterine
           horns
 Uterus –attachment site of placenta
 Cervix – elastic muscle protects uterus
  from contaminants
 Vagina – female organ of copulation
  connecting the vulva to cervix; elastic
  tissue for birth canal
 Vulva – external
  opening of
  reproductive canal
 Cow: almond shaped
  ovaries; one larger than the
  other
 Ewe/Doe: almond shaped
  ovaries; half the size of the
  cow
 Mare: kidney shaped
  ovaries; three times larger
  than cow
 Sow: cluster of grapes
  shaped ovaries; half the size
  of the cow
 Bicornuate uterus: 80 – 90%
  is uterine horn; sow, cow,
  doe, ewe
 Bipariite uterues: 80 – 90% is
  uterine body; mare
   Functions:
    › Hormonal Control
    › Transport Sperm
    › Produce Gametes
    › Facilitate Fertilization
    › Nourish Embryo and Fetus
    › Participate in Parturition
    › Recycle
   Polyestrous – cycle year round: cow, sow
   Seasonally polyestrous – cycle in one
    season, anestrus in another: Horse
    (spring/summer), ewe and doe (fall/winter)
   Monestrous – cycle once then anestrous:
    dog
   Monotocous - one offspring each gestation
    period: cow, mare, doe, and ewe
   Polytocous – multiple offspring each
    gestation period: sow
   Testes – sperm and testosterone production
   Epididymis – transport, maturation and storage
   Ductus deferens – transport and ejaculation
   Penis - copulation
   Accessory Sex Glands
    › Prostate – secrete neutralizing liquid
    › Bulbourethral gland – secrete liquid
       lubricant or gelatinous plug
    › Seminal vesicles – secrete liquid
      lubricant
    › Ampulla – glandular enlargement of
      ductus deferens
   Thermoregulation controlled by
    Scrotum and blood supply
 Musculocavernous penis – muscular tissue
  fills with blood during erection
 Fibroelastic penis – fibrous tissue elongates
  during erection by straightening of
  sigmoid flexure
 Bull, Stallion, Ram,
  Buck have all
  accessory sex glands
 Boar has corkscrew
  shaped penis and no
  ampulla
1. Ovulation
2. Estrus
3. Fertilization
4. Gestation
5. Parturition
6. Lactation
 Estrus (Follicular) –
  period when female is
  sexually receptive to
  male, the “heat” stage;
  growth, maturation,
  and ovulation of
  follicles
 Diestrus (Luteal) –
  period when female is
  not sexually receptive;
  growth and function of
  corpus luteum
 Helps the female
  physically prepare for
  conception
 Anestrus – no ovarian
  activity
1. Growing follicle
2. Maturing follicle
3. Ovulation
4. Formation of
   Corpus Luteum
5. Regressing
   Corpus Luteum
   Estrogen – induces changes           Progesterone – induces
    associated with estrus                changes associated with
    › Produced by follicles of            pregnancy
      ovary                               › Produced by corpus luteum
    › Makes female more                     and placenta
      receptive toward male               › Decreases the receptiveness of
    › Prepares female for                   the female to the male
      conception physically               › Causes cervix to close
    › Increases cervical relaxation       › At its lowest level during the
      and uterine resistance to             estrus phase
      infection                           › Suppresses hormonal activity to
    › Peaks 1-2 days before                 maintain pregnancy (if female
      ovulation                             is pregnant)
                                          › If female is not pregnant, the
                                            estrous cycle will continue
   Luteinizing hormone (LH)
    › Secreted by the
      pituitary
    › Assists the maturation of
      the follicle, ovulation
      and formation of corpus
      luteum
   Follicle stimulating
    hormone (FSH)
    › Secreted by pituitary,
      transported through
      bloodstream to ovaries
    › Stimulates the
      production of follicles
    › Can be given to cause
      superovulaiton
    Ovulation – follicle releases female gamete,
         the oocyte
     Corpus Luteum – formed from the follicle after oocyte is
      released
     Spontaneous Ovulator – ovulation controlled by estrus
      cycle
        Induced ovulation - after copulation
         › Seasonal: cat
         › Non-seasonal: llama, rabbit
         Length of Estrus   Timing of Ovulation               Timing of Insemination
Cattle   12-19 hours        10-11 hours (after end of         7-18 hours (after onset of estrus)
                            estrus)
Horse    2-11 days          1-2 days (before end of estrus)   2nd day of estrus and every 2 days of
                                                              rest of estrus.
Sheep    24-36 hours        24-36 hours (after onset of       12-18 hours (after onset of estrus)
                            estrus)
Swine    48-72 hours        35-45 hours (after onset of       16-24 hours (after onset of estrus) then
                            estrus)                           8-24 hours later
 Fertilized oocyte travels through oviduct, going through
  mitotic divisions
 Morula – solid ball of cells; stage where embryo enters the
  uterus
 Blastocyst – hollow ball of cells; stage where embryo
  attaches to wall of uterus
 Membranes of embryo interdigitate with endometrium to
  form placenta
   Four Fetal Membranes – blood,
    nutrient and antibody transfer
    › Yolk sac – nutrient supply
    › Amnion –parturition
      lubrication
    › Allantois and Chorion
      fusion – uterus
      attachment
   Epitheliochorial – maternal epithelium adjacent
    to embryonic chorion
   Diffuse – entire surface forms attachments:
    horse, pig
   Cotyledonary – placental attachements in
    specifc areas: ruminants
   Placentome – attachment of cotyledon and
    caruncle
    › Cotyledons – fetal side of placenta
    › Caruncles – maternal side of placenta
 After Fertilization, embryo at blastocyst
         stage implants in uterine wall and
         develops placenta
        Gestation is maintained by the hormone
         progesterone
            › Progesterone – produced by Corpus Luteum
                and Placenta
           Source of Progesterone                                                            Gestation
Goat       All from CL                                                                       150 days

Sheep      CL to day 50 then placenta                                                        150 days
Cow        CL throughout gestation. However, after ~ day 200 placenta                        280 days
           and adrenal are producing enough progesterone to maintain pregnancy.
Sow        All from CL                                                                       114 days
Mare       1st CL produces progesterone up to ~ 180 days, FSH stimulates follicle            335 days
           growth and eCG causes CL's to form from follicles. They secrete
           progesterone and are active from day 40-180. Progesterone is low until 270
           days- rises in last 5 months due probably to placental progesterone production.
   Parturition – Birth process
    › Stage 1 – Dilation of cervix
    › Stage 2 – Expulsion of fetus
    › Stage 3 – Expulsion of placenta
   Lactation – Mammary development and Milk
    secretion and ejection
    › Colostrum – first milk produced, containing high protein, fat,
        vitamin A, minerals, and antibodies
   Hormones
    › Prolactin – produced by pituitary; stimulates mammary
      gland formation and lactation
    › Oxytocin – produced by pituitary; stimulates milk let down,
      uterine contraction and prostaglandin release
    › Relaxin – produced by Corpus Luteum to relax cervix at
      parturition
   Straight Breeding – produces purebred
    animals
    › Purebred
    › Inbreeding (closely related animals)
    › Line breeding (distant relatives)
    › Outcrossing (unrelated animals)
    › Grading up (purebred mated to crossbred)
   Cross Breeding – produces hybrid animals
    › Two Breed Cross
    › Three Breed Rotation (sires rotated)
   Live Cover – methods in which male and
    female are physically brought together
    to breed
    › Natural Mating – random mating of males
     and females turned out together
       Family Selection
       Pedigree Selection
       Progeny Selection
   Artificial Insemination
    (AI) – female is
    inseminated by a
    veterinarian
   Embryo Transfer –
    removing the embryo
    from a female and
    transferring it to a
    surrogate female

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Comparative Reproductive Anatomy

  • 1.
  • 2. Bovine  Ovary – primary reproductive organ, produces gametes and sex hormones  Oviduct – transport sperm to ovum and embryo to uterine horns
  • 3.  Uterus –attachment site of placenta  Cervix – elastic muscle protects uterus from contaminants  Vagina – female organ of copulation connecting the vulva to cervix; elastic tissue for birth canal  Vulva – external opening of reproductive canal
  • 4.  Cow: almond shaped ovaries; one larger than the other  Ewe/Doe: almond shaped ovaries; half the size of the cow  Mare: kidney shaped ovaries; three times larger than cow  Sow: cluster of grapes shaped ovaries; half the size of the cow  Bicornuate uterus: 80 – 90% is uterine horn; sow, cow, doe, ewe  Bipariite uterues: 80 – 90% is uterine body; mare
  • 5. Functions: › Hormonal Control › Transport Sperm › Produce Gametes › Facilitate Fertilization › Nourish Embryo and Fetus › Participate in Parturition › Recycle
  • 6. Polyestrous – cycle year round: cow, sow  Seasonally polyestrous – cycle in one season, anestrus in another: Horse (spring/summer), ewe and doe (fall/winter)  Monestrous – cycle once then anestrous: dog  Monotocous - one offspring each gestation period: cow, mare, doe, and ewe  Polytocous – multiple offspring each gestation period: sow
  • 7. Testes – sperm and testosterone production  Epididymis – transport, maturation and storage  Ductus deferens – transport and ejaculation  Penis - copulation  Accessory Sex Glands › Prostate – secrete neutralizing liquid › Bulbourethral gland – secrete liquid lubricant or gelatinous plug › Seminal vesicles – secrete liquid lubricant › Ampulla – glandular enlargement of ductus deferens  Thermoregulation controlled by Scrotum and blood supply
  • 8.  Musculocavernous penis – muscular tissue fills with blood during erection  Fibroelastic penis – fibrous tissue elongates during erection by straightening of sigmoid flexure  Bull, Stallion, Ram, Buck have all accessory sex glands  Boar has corkscrew shaped penis and no ampulla
  • 9. 1. Ovulation 2. Estrus 3. Fertilization 4. Gestation 5. Parturition 6. Lactation
  • 10.  Estrus (Follicular) – period when female is sexually receptive to male, the “heat” stage; growth, maturation, and ovulation of follicles  Diestrus (Luteal) – period when female is not sexually receptive; growth and function of corpus luteum  Helps the female physically prepare for conception  Anestrus – no ovarian activity
  • 11. 1. Growing follicle 2. Maturing follicle 3. Ovulation 4. Formation of Corpus Luteum 5. Regressing Corpus Luteum
  • 12. Estrogen – induces changes  Progesterone – induces associated with estrus changes associated with › Produced by follicles of pregnancy ovary › Produced by corpus luteum › Makes female more and placenta receptive toward male › Decreases the receptiveness of › Prepares female for the female to the male conception physically › Causes cervix to close › Increases cervical relaxation › At its lowest level during the and uterine resistance to estrus phase infection › Suppresses hormonal activity to › Peaks 1-2 days before maintain pregnancy (if female ovulation is pregnant) › If female is not pregnant, the estrous cycle will continue
  • 13. Luteinizing hormone (LH) › Secreted by the pituitary › Assists the maturation of the follicle, ovulation and formation of corpus luteum  Follicle stimulating hormone (FSH) › Secreted by pituitary, transported through bloodstream to ovaries › Stimulates the production of follicles › Can be given to cause superovulaiton
  • 14. Ovulation – follicle releases female gamete, the oocyte  Corpus Luteum – formed from the follicle after oocyte is released  Spontaneous Ovulator – ovulation controlled by estrus cycle  Induced ovulation - after copulation › Seasonal: cat › Non-seasonal: llama, rabbit Length of Estrus Timing of Ovulation Timing of Insemination Cattle 12-19 hours 10-11 hours (after end of 7-18 hours (after onset of estrus) estrus) Horse 2-11 days 1-2 days (before end of estrus) 2nd day of estrus and every 2 days of rest of estrus. Sheep 24-36 hours 24-36 hours (after onset of 12-18 hours (after onset of estrus) estrus) Swine 48-72 hours 35-45 hours (after onset of 16-24 hours (after onset of estrus) then estrus) 8-24 hours later
  • 15.  Fertilized oocyte travels through oviduct, going through mitotic divisions  Morula – solid ball of cells; stage where embryo enters the uterus  Blastocyst – hollow ball of cells; stage where embryo attaches to wall of uterus  Membranes of embryo interdigitate with endometrium to form placenta
  • 16. Four Fetal Membranes – blood, nutrient and antibody transfer › Yolk sac – nutrient supply › Amnion –parturition lubrication › Allantois and Chorion fusion – uterus attachment
  • 17. Epitheliochorial – maternal epithelium adjacent to embryonic chorion  Diffuse – entire surface forms attachments: horse, pig  Cotyledonary – placental attachements in specifc areas: ruminants  Placentome – attachment of cotyledon and caruncle › Cotyledons – fetal side of placenta › Caruncles – maternal side of placenta
  • 18.  After Fertilization, embryo at blastocyst stage implants in uterine wall and develops placenta  Gestation is maintained by the hormone progesterone › Progesterone – produced by Corpus Luteum and Placenta Source of Progesterone Gestation Goat All from CL 150 days Sheep CL to day 50 then placenta 150 days Cow CL throughout gestation. However, after ~ day 200 placenta 280 days and adrenal are producing enough progesterone to maintain pregnancy. Sow All from CL 114 days Mare 1st CL produces progesterone up to ~ 180 days, FSH stimulates follicle 335 days growth and eCG causes CL's to form from follicles. They secrete progesterone and are active from day 40-180. Progesterone is low until 270 days- rises in last 5 months due probably to placental progesterone production.
  • 19. Parturition – Birth process › Stage 1 – Dilation of cervix › Stage 2 – Expulsion of fetus › Stage 3 – Expulsion of placenta  Lactation – Mammary development and Milk secretion and ejection › Colostrum – first milk produced, containing high protein, fat, vitamin A, minerals, and antibodies  Hormones › Prolactin – produced by pituitary; stimulates mammary gland formation and lactation › Oxytocin – produced by pituitary; stimulates milk let down, uterine contraction and prostaglandin release › Relaxin – produced by Corpus Luteum to relax cervix at parturition
  • 20. Straight Breeding – produces purebred animals › Purebred › Inbreeding (closely related animals) › Line breeding (distant relatives) › Outcrossing (unrelated animals) › Grading up (purebred mated to crossbred)  Cross Breeding – produces hybrid animals › Two Breed Cross › Three Breed Rotation (sires rotated)
  • 21. Live Cover – methods in which male and female are physically brought together to breed › Natural Mating – random mating of males and females turned out together  Family Selection  Pedigree Selection  Progeny Selection
  • 22. Artificial Insemination (AI) – female is inseminated by a veterinarian  Embryo Transfer – removing the embryo from a female and transferring it to a surrogate female

Editor's Notes

  1. http://www.drostproject.org/en_bovrep/guide.html
  2. http://animalsciences.missouri.edu/reprod/Notes/index.htm
  3. http://nongae.gsnu.ac.kr/~cspark/teaching/chap2.html
  4. For more information on hormones: http://www.equine-reproduction.com/articles/estrous.htm
  5. http://www.vivo.colostate.edu/hbooks/pathphys/reprod/index.html
  6. http://nongae.gsnu.ac.kr/~cspark/teaching/chap9.html
  7. http://www.dpi.nsw.gov.au/__data/assets/pdf_file/0006/148038/Beef-cattle-breeding-systems.pdf
  8. The AI procedure: http://www.smartlilplayer.com/html/ai.htmlhttp://www.ansci.wisc.edu/jjp1/ansci_repro/misc/websites09/thur/Ovsynch/Ovsynch.htm