This document outlines the facilities and procedures for collecting semen from stallions for artificial insemination. It describes the collection area as a clean, safe space with good footing and restraint equipment located near the laboratory. The collection supplies include an artificial vagina, collection bottle, and thermal covering. Semen is checked for volume, motility, morphology, and concentration in the laboratory using an incubator, microscope, and densimeter. Mares are typically inseminated with 500 million sperm and checked via ultrasound for pregnancy 14 days later.

1. Artificial Insemination in the
Equine
Collection Facility and Laboratory

2. Collection of Stallion

3. Collection Area
• Clean and safe, good footing
• Phantom or dummy “mare”
• In close proximity to laboratory
• Restraint equipment (hobbles, twitch, etc.)
• Cleaning and collection supplies for
stallion and mare
• 1. Each stallion should have separate bucket
• 2. Artificial Vagina and parts. Water holding cylinder with
handle, inner removable liner, collection bottle with filter to
trap gel fraction, thermal covering for collection bottle.

4. Phantom

5. Collection Supplies

6. Restraint Equipment
• “Using Mare as Jump Mare”

7. Stallion Restraint

8. Cleaning Stallion Penis
“Clean warm water”

9. Laboratory Supplies
• Check Semen Volume (average 30-120ml)

10. Incubator
• 100 Fahrenheit or 38 Celsius

11. Morphology and Motility
>65% progressive motility (average)

12. Semen Concentration
average - 150 to 250 million sperm/ml.
Densimeter

13. Inseminate Mare
500million sperm per inseminate

14. 14 Day Ultrasound Preg-Check