The document summarizes a stem cell technology certification program at City College of San Francisco (CCSF). The 18-month program trains students in specialized skills for stem cell research careers through courses covering topics like mammalian cell culture, fluorescent cell technology, and stem cell technology. It has trained over 150 students, many of whom have been hired at top research institutions. The program has evolved since 2002 and now includes an NSF-funded initiative to develop a cross-disciplinary curriculum on stem cell research for implementation at multiple colleges.

1. Biotechnology Program Stem Cell Technology Certification Paul A. Raju, D.Phil

2. Objective Educate and train students with the specialized skills required for jobs in the lucrative and rapidly growing field of stem cell research Launched in 2006

3. Course Structure BTEC21A: Mammalian Cell Culture (6 weeks/9 hours per week) BTEC21B: Fluorescent Cell Technology (6 weeks/9 hours per week) BTEC21C: Stem Cell Technology (8 weeks/9 hours per week) Pre-requisite - 1 semester of Biology with lab and 2 semesters of Chemistry including Organic with lab BTEC24 & 25: Introductory and Advanced PCR BTEC22: ELISA BTEC23: Western Blotting Text - Culture of Animal Cells: A manual of basic technique (Ian Freshney) Fall, Spring, and Summer. Day and Night Classes ~1 calendar year to complete

4. CCSF Cell Culture Facility

5. Instrumentation 2 - Flow Cytometers (BD FACScans) 6 - Epifluorscence Inverted Microscopes 10 - Inverted Microscopes 6 - CO2 Incubators & 6 Laminar Flow Hoods

8. Understand (principles) and operate a flow cytometry

10. Evolution of the Program 2002 NSF-ATE funded “Fix a Gene” 2004 Prop 71 - $3 billion for Stem Cell Research ( California Initiative for Regenerative Medicine, CIRM) 2005 WIA CA Governor’s grant 2005 Creation of Cell Culture Laboratory 2006 Launching of Stem Cell Certificate Program 2007 First human ES cell training at UCSF 2009 CIRM Grant

11. A Novel Curriculum for Training in Stem Cell Research NSF CCLI Grant 0920799: Workforce Development in STEM Cell Research Christopher Thomas Scott, Stanford University, PI Aims Develop a multidisciplinary undergraduate curriculum for community and four year colleges for workforce training in stem cell research. Cross-disciplinary design: basic and applied science, lab skills, and ethics, law, and policy Pilot first module in four colleges (SPRING, 2011) Deploy suite of measurements (summative and formative assessements)

12. NSF CCLI Grant: Workforce Development in STEM Cell Research Content Developers* and Instructors DritanAgilou* Stanford University Paul AnandaRaju City College of San Francisco Mariluci Blandon Middlesex Community College Jessica Byrne* Art and Illustration KatayounChamany Eugene Lang College Lily Cheng San Francisco State College Eric Chiao* Roche Biosciences Tyler Cutforth* University of California, Santa Cruz Nicole Holthius* Assessment Development Thorsten Schlager* Harvard University Carin Zimmerman* City College of San Francisco

13. NSF CCLI Grant: Workforce Development in STEM Cell Research Year 1 Progress: > 80 hours of Instruction and Materials Primers: Short topical introductions for students Slide Presentations: Fully-illustrated slide sets with teacher’s notes and image library Activities: Cross-topic, multi-week activities test essential concepts and reflective learning Integrated Laboratories: Fully immersive labs teaching essential skills in stem cell biology Assessment Suite: Embedded, formative, and summative measurements

14. Acknowledgements CCSF Phil Jardim Kristin Hershbell Dr. Carin Zimmerman Dr. GolnarAfshar Dr. Alex Zambon Dr. Jennifer Ng Dr. Edith Kaeuper Dr. Wing Tsao Dr. Carole Toebe Dr. Elaine Johnson UCSF/Gladstone Dr. Mort Cowan Ms. Elizabeth Dunn Dr. Bruce Conklin Dr. Jennifer Ng Dr. Susan Fisher MiltenyiBiotech Mr. Thad Baker Ms. Julie Rae Dr. Shane Oram