This study examined the roles of acceptance and suppression in relaxation. 95 participants underwent a stressor task designed to induce anxiety before being given different interventions promoting acceptance, suppression, mindfulness, or endurance. Physiological arousal and self-reported anxiety were measured. Results showed that acceptance and mindfulness led to lower physiological arousal compared to suppression and a placebo. Acceptance also led to lower self-reported anxiety over time compared to other conditions. The findings provide evidence for the role of acceptance in relaxation and reinforce that suppression can paradoxically increase anxiety. Further research is needed to deepen understanding of the underlying processes.