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This document summarizes the effects of various enzymes on blood antigens. It notes that the M and N antigens are easily destroyed by common blood bank enzymes like ficin, papain and bromelin. The S and s antigens are also degraded by these enzymes but to a lesser extent and depending on enzyme strength. Fy a and Fy b antigens are destroyed by proteolytic enzymes like ficin, papain, bromelin and chymotrypsin. In contrast, treatment with ficin and papain enhances the reactivity of I and i antigens with antibodies. While the P1 antigen is resistant to enzyme treatment, the reactivity of antibodies can be enhanced against enzyme-treated cells. Similarly, papain and fic
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This document discusses a study aiming to elucidate the mechanism by which the HDAC inhibitor Apicidin induces expression of the drug efflux pump P-glycoprotein (P-gp), which may lead to multidrug resistance. The study found that Apicidin treatment activated the PI3K pathway, leading to phosphorylation of the transcription factor SP1. Phosphorylated SP1 then signals chromatin remodeling and activation of the P-gp promoter. This occurred independently of changes in DNA methylation and required the Y-box and GC box regions of the promoter. Blocking the PI3K pathway prevented Apicidin-induced P-gp expression.
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みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
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Chapter 8, objective
- 1. Chapter 8, objective # 17 List the antigens that are denatured by routine blood bank enzymes and antigens whose reactivity with antibody is enhanced with enzymes?
- 2. Antigens denatured activity by enzymes M and N antigens Easily destroyed by the routine blood bank enzymes ficin, papain, and bromelin. Less common enzymes are trypsin and pronase. S and s antigen Less easily degraded by Ficin, papain, bromelin, pronase, and chymotrypsin can destroy S and s activity, but the amount of degradation may depend on the strength of the enzyme solution.
- 3. Fya and Fyb antigen Antigens are destroyed by proteolytic enzymes such as ficin, papain, bromelin, and chymotrypsin, and by ZZAP. Neuramidase may reduce the molecular weight of Fya and Fyb antigen, but it does not destroy antigenic activity and neither does purified trypsin.
- 4. Antigens enhanced activity by enzymes I and i antigen Treatment of RBCs with ficin and papain enhances reactivity of I and I antigens with their respective antibodies. The I and i antigens are resistant to treatment with DTT and glycine- acid EDTA. P1 It is resistant to different enzymes treatment. Reactivity of the antibodies can be greatly enhanced by testing with enzyme-treated RBCs.
- 5. Jka and Jk b antigen Kidd antigens are not denatured by papain or ficin; treatment of RBCs with enzymes generally enhances reactivity with Kidd antibodies.