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Challenges in vascular tissue engineering
- 1. Challenges in Vasculartissue Engineering Presented by: Yogesh Joshi
- 2. Introduction • Growing bloodvessel in vivo or in vitro and transferring to the required site • Example: to treat atherosclerosis leading to Myocardial infarction,coronary artery bypass graft surgery
- 3. Steps Involved • FindingSubstitute Material eg. Dacron or expanded polytetrafluoroethylene (ePTFE). • Endothelial Cell seeded synthetic graft • Cell technology :Cell sourcing and the control and manipulation of cell function. • Construct Technology: Mimicking the natural vessel in architecture and function • Integrating living substitute in living system
- 4. Five approaches used: •EC-seeded synthetic grafts- Adherence of EC • Collagen-based blood vessel analogs- Physical weakness • Biodegradable synthetic polymer-based constructs-expensive and vasoelasticity • Cell self-assembly blood vessels: long culture time, elasticity and integrity • Decellularized tissue approaches: Lack of non-thromogenics components
- 5. Methods and respectivechallenges Cellular Mimicking biochemical and mechanical conditions Lack of vasoactive responses Physical barrier in cellular environment Acellular Risk of Contamination Lack EC lining leading to thrombus formation Recruiting cells
- 6. Major Challenges: • Providingnon-thrombogenic surface • Immunogenicity • Choosing a right chemical agonist, mechanical environment and genetic engineering approach • Reproducibility and preservation of the construct • Physiological stress
- 7. Possible remedies: • Arg-Gly-Asp(RGD) peptide sequence as an adhesive coating • Choosing allogenic cells over xenogenic • Glycation of collageneous matrix
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