This study identified regulatory miRNAs associated with cervical cancer metastasis. Several miRNAs were found to be upregulated in metastatic cervical cancer tissue compared to normal tissue, including miR-183-5p, miR-200b-5p, miR-15b-5p, miR-425-5p, miR-96-5p, miR-18a-5p, miR-93-5p, miR-16-5p, and miR-92a-3p. miR-133b was commonly downregulated. miRNA enrichment analysis revealed an enrichment of the miR-17 family. Pathway analysis found the cell cycle pathway to be most significant by KEGG, and gene expression to be most significant by Reactome

1. Identification of Regulatory
miRNAs in Cervical Cancer
Metastasis
Satarupa Banerjee and Karunagaran Devarajan
Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, IIT
Madras, Chennai-600036
ICBHE-2018

2. Cervical Cancer Definition and Statistics
• Cervical cancer is cancer that begins in the uterine cervix, the lower end of the
uterus that contacts the upper vagina
• Cervical cancer is the fourth most common cancer in women, and the seventh
overall

3. Cervical Cancer Metastasis

4. RNAs in Cervical Cancer
Wang et al. Front. Microbiol., 19 September 2017
There are different types of RNAs, of
which a few are being studied
nowadays extensively
• mRNA
• miRNA
• lncRNA
• circRNA

5. miRNAs in Cervical Cancer
 MicroRNAs are small (~18-24 nt), non-coding RNAs that regulate gene
expression & associated with cancer

6. Global Identification of Precise Metastasis Related miRNAs
miRNA
Identification
TCGA-CESC
DE miRNA
GSE102969
DE miRNA
3 non metastatic
and 3 lymph node
metastatic CC
Tissue
Normal
and CC
Target
Transcription
Factor Mining
Pathway
analysis
Methodology
KEGG
Reactome
WikiPathway
Find Common miRNAs
miRNA Family
Enrichment
Analysis

7. Result

8. miRNA Family Enrichment Analysis

9. KEGG Pathway Analysis

10. Reactome Pathway Analysis

11. WikiPathway Analysis

12. Target Transcription Factor Mining
Reactome 2016 of modulating TFs

13. Role of selected miRNAs in TGF-β pathway

14. Take Home Message
 Results suggest that hsa-miR-183-5p, hsa-miR-200b-5p, hsa-miR-15b-5p, hsa-miR-
425-5p, hsa-miR-96-5p, hsa-miR-18a-5p, hsa-miR-93-5p, hsa-miR-16-5p, hsa-miR-
106b-5p and hsa-miR-92a-3p were upregulated, while hsa-miR-133b was commonly
downregulated.
 miRNA enrichment analysis showed miR-17 family enrichment
 During KEGG analysis, Cell Cycle was found to be most significant pathway, while
Reactome analysis showed Gene Expression to be the most significant pathway.
 Target mRNA pathway analysis showed “Downregulation of TGF-β receptor
signalling” as one of the significant pathways. In Reactome 2016 analysis of target
TFs, Loss of Function of TGFBR1 in Cancer_Homo sapiens_R-HSA-1912422 was
also important, besides Pre-NOTCH Transcription and Translation_Homo
sapiens_R_HSA-1912408 being the most significant pathway
 Differentially upregulated metastasis associated miRNAs, like hsa-miR- 18a-5p,
hsa-miR-92a-3p, hsa-miR-93a-5p and hsa-miR-15b-5p may act via TGF-β
pathway

15. Thank You