Centrifuguation_applied_biology_bio.pptx
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There are three main types of centrifugation used to separate particles: differential centrifugation separates based on size and density, density gradient centrifugation uses density gradients for finer separation such as of biomolecules, and ultracentrifugation is a high-speed technique used to separate small particles at a molecular level. Differential centrifugation is used to isolate organelles from cells through multiple rounds of centrifugation, density gradient centrifugation separates particles by density as they move through gradients, and ultracentrifugation achieves extremely high speeds for separating macromolecules and proteins. Centrifugation techniques are useful tools in applied biology for studying subcellular structures and isolating components.
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This document summarizes differential and density gradient centrifugation. It defines centrifugation as a process used to separate materials suspended in a liquid medium using centrifugal force. It then discusses the principle behind centrifugation, where denser components migrate away from the axis. It describes the instrumentation, including low-speed centrifuges used for routine sedimentation, high-speed centrifuges for more sophisticated applications, and ultracentrifuges generating intense heat. It also outlines types of centrifugation techniques like density gradient and differential centrifugation and their uses. Applications include separating blood components, precipitated proteins, and subcellular organelles.
Density gradient centrifugation
Density gradient centrifugation is currently considered the gold-standard method for achieving the highest-purity exosome samples, as they remove non-specifically bound proteins from vesicles
Density gradient centrifugation
Density gradient centrifugation is a technique used to separate particles based on density. It involves placing a sample on a preformed density gradient, such as sucrose or cesium chloride, and centrifuging. Under centrifugation, particles band within the gradient according to their density. There are two types of density gradient centrifugation - rate zonal centrifugation, which separates particles of differing sizes, and isopycnic centrifugation, which separates particles solely based on density. Density gradient centrifugation has many applications, including purification of viruses, bacteria, proteins, and separation of biomolecules.
Priti Khadse Seminar M.Sc-BT 1st year.pptx
Dear students I hope this PPT finds you well. This presentation is all about the laboratory instrument centrifuge and its working called centrifugation. A centrifuge is a laboratory device used to separate fluids, gases, or liquids of different densities by spinning them at high speeds. It operates on the principle of centrifugal force, causing heavier particles or substances to move outward and settle at the bottom while lighter ones move toward the top. This separation process is employed in various fields such as medicine, biology, chemistry, and industry for tasks like separating blood components, purifying samples, and separating mixtures based on density variances. Centrifuges come in various types, including microcentrifuges, ultracentrifuges, and refrigerated centrifuges, each designed for specific applications requiring different speeds and capacities.Types of centrifuges and their functions include:
Microcentrifuge:
Function: Used for small-volume samples (typically ranging from 0.2 mL to 2.0 mL) in molecular biology, biochemistry, and clinical applications. It separates cellular components, proteins, DNA, and RNA.
Clinical Centrifuge:
Function: Primarily used in medical laboratories for processing blood, urine, and other bodily fluids. It separates components like red blood cells, plasma, and serum for diagnostic purposes.
Refrigerated Centrifuge:
Function: Similar to standard centrifuges but equipped with cooling systems to maintain low temperatures during separation. Ideal for samples sensitive to heat, like enzymes or biological materials.
Ultracentrifuge:
Function: Operates at extremely high speeds, separating particles at molecular levels. Used for studying macromolecules, lipoproteins, and subcellular particles.
High-Speed Centrifuge:
Function: Used in research labs for general separation tasks, capable of higher speeds than standard centrifuges. It's versatile and employed across various scientific disciplines.
Differential Centrifuge:
Function: Separates particles based on their sedimentation rates and size differences. It's used to isolate specific components from complex mixtures.
Preparative Centrifuge:
Function: Focuses on large-scale sample separation for purification purposes. It's employed in industrial settings for isolating biomolecules or other substances at higher capacities.
Centrifuges find applications in various fields such as molecular biology, biochemistry, medicine, pharmaceuticals, and industrial processes. Their versatility in separating substances based on density variances makes them invaluable tools in scientific research, diagnostics, and manufacturing processes.
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Centrifugation is a analytical technique widely used in biochemistry and molecular biology for separation purposes.
Centrifugation principle and types by Dr. Anurag Yadav
concept of cnetrifugation,
basic Principle
centrifugal force
types of centrifugation based on use and rotor type
application of the each type of centrifuge
Ultracentrifuge in detail
application in general
Centrifugation by Dr. Anurag Yadav
Centrifuagtion technique, principle, types of centrifugation, application of each type of centrifugation.
Centrifugation
Centrifugation is a process that uses centrifugal force to separate mixtures of particles based on density. It works by spinning a sample rapidly, causing denser particles to migrate outward while less dense particles move inward. There are various types of centrifuges and rotors that can be used for different applications like separating blood components, purifying proteins, or clarifying wine. Common techniques include differential centrifugation to separate cell components, density gradient centrifugation to separate mixtures based on buoyant density, and ultracentrifugation for high-speed separations. Centrifugation is widely used in industries like water treatment and laboratories for analytical purposes.
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This document summarizes differential and density gradient centrifugation. It defines centrifugation as a process used to separate materials suspended in a liquid medium using centrifugal force. It then discusses the principle behind centrifugation, where denser components migrate away from the axis. It describes the instrumentation, including low-speed centrifuges used for routine sedimentation, high-speed centrifuges for more sophisticated applications, and ultracentrifuges generating intense heat. It also outlines types of centrifugation techniques like density gradient and differential centrifugation and their uses. Applications include separating blood components, precipitated proteins, and subcellular organelles.
Density gradient centrifugation
Density gradient centrifugation is currently considered the gold-standard method for achieving the highest-purity exosome samples, as they remove non-specifically bound proteins from vesicles
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Density gradient centrifugation is a technique used to separate particles based on density. It involves placing a sample on a preformed density gradient, such as sucrose or cesium chloride, and centrifuging. Under centrifugation, particles band within the gradient according to their density. There are two types of density gradient centrifugation - rate zonal centrifugation, which separates particles of differing sizes, and isopycnic centrifugation, which separates particles solely based on density. Density gradient centrifugation has many applications, including purification of viruses, bacteria, proteins, and separation of biomolecules.
Priti Khadse Seminar M.Sc-BT 1st year.pptx
Dear students I hope this PPT finds you well. This presentation is all about the laboratory instrument centrifuge and its working called centrifugation. A centrifuge is a laboratory device used to separate fluids, gases, or liquids of different densities by spinning them at high speeds. It operates on the principle of centrifugal force, causing heavier particles or substances to move outward and settle at the bottom while lighter ones move toward the top. This separation process is employed in various fields such as medicine, biology, chemistry, and industry for tasks like separating blood components, purifying samples, and separating mixtures based on density variances. Centrifuges come in various types, including microcentrifuges, ultracentrifuges, and refrigerated centrifuges, each designed for specific applications requiring different speeds and capacities.Types of centrifuges and their functions include:
Microcentrifuge:
Function: Used for small-volume samples (typically ranging from 0.2 mL to 2.0 mL) in molecular biology, biochemistry, and clinical applications. It separates cellular components, proteins, DNA, and RNA.
Clinical Centrifuge:
Function: Primarily used in medical laboratories for processing blood, urine, and other bodily fluids. It separates components like red blood cells, plasma, and serum for diagnostic purposes.
Refrigerated Centrifuge:
Function: Similar to standard centrifuges but equipped with cooling systems to maintain low temperatures during separation. Ideal for samples sensitive to heat, like enzymes or biological materials.
Ultracentrifuge:
Function: Operates at extremely high speeds, separating particles at molecular levels. Used for studying macromolecules, lipoproteins, and subcellular particles.
High-Speed Centrifuge:
Function: Used in research labs for general separation tasks, capable of higher speeds than standard centrifuges. It's versatile and employed across various scientific disciplines.
Differential Centrifuge:
Function: Separates particles based on their sedimentation rates and size differences. It's used to isolate specific components from complex mixtures.
Preparative Centrifuge:
Function: Focuses on large-scale sample separation for purification purposes. It's employed in industrial settings for isolating biomolecules or other substances at higher capacities.
Centrifuges find applications in various fields such as molecular biology, biochemistry, medicine, pharmaceuticals, and industrial processes. Their versatility in separating substances based on density variances makes them invaluable tools in scientific research, diagnostics, and manufacturing processes.
_centrifugation .pptx
Centrifugation is a analytical technique widely used in biochemistry and molecular biology for separation purposes.
Centrifugation principle and types by Dr. Anurag Yadav
concept of cnetrifugation,
basic Principle
centrifugal force
types of centrifugation based on use and rotor type
application of the each type of centrifuge
Ultracentrifuge in detail
application in general
Centrifugation by Dr. Anurag Yadav
Centrifuagtion technique, principle, types of centrifugation, application of each type of centrifugation.
Centrifugation
Centrifugation is a process that uses centrifugal force to separate mixtures of particles based on density. It works by spinning a sample rapidly, causing denser particles to migrate outward while less dense particles move inward. There are various types of centrifuges and rotors that can be used for different applications like separating blood components, purifying proteins, or clarifying wine. Common techniques include differential centrifugation to separate cell components, density gradient centrifugation to separate mixtures based on buoyant density, and ultracentrifugation for high-speed separations. Centrifugation is widely used in industries like water treatment and laboratories for analytical purposes.
Centrifugation-Basic study
This PPt is useful for the people who want to study the basics and the types of centrifugation and parts of the centrifuge
JAndrew/TYPES OF CENTRIFUGATION.pptx
This document describes different types of centrifugation techniques used to separate particles based on their properties. It discusses analytical centrifugation which separates particles based on density and centrifugal force. Density gradient centrifugation uses a gradient medium to separate particles based on differences in buoyant density. Differential centrifugation separates particles into pellets of increasing size through successive centrifugation steps with increasing force.
Centrifugation notes slideshare
Centrifugation is a technique that uses centrifugal force to separate components of a solution based on properties like density, size, and shape. It works by spinning the solution at high speeds, allowing heavier components to sediment to the bottom. There are two main types - density gradient centrifugation, where particles separate through a density gradient, and differential centrifugation, which separates organelles from cells. Centrifugation has many applications, including separating cream from milk, clarifying wine, and purifying proteins and cells.
Ultra centrifugation
Ultracentrifugation is a technique that uses very high rotational speeds, up to 80,000 rpm, to separate particles via centrifugal force up to 600,000g. There are two main types: analytical ultracentrifugation monitors particles in real-time to study molecular interactions and properties, while preparative ultracentrifugation isolates and purifies particles like organelles. Common techniques include differential centrifugation to separate organelles and density gradient centrifugation to separate mixtures based on density.
Applied Biochemistry
This document discusses centrifugation, which uses centrifugal force to separate biological particles in liquid. It describes the basic principles of how centrifugation works based on gravity and centrifugal force. There are different types of rotors and centrifuges that are used for various purposes. Differential centrifugation separates particles based on size, while density gradient centrifugation uses layers of different density solutions to separate particles based on density. Analytical centrifugation uses high speeds and optical systems to analyze particles. Centrifugation has many applications including separating blood cells, precipitates, subcellular components, and more.
North south university
Centrifugation is one of
the most important and
widely applied research cellular,
techniques in bio-chemistry and
molecular biology, pharmacy and
in medicine.
Centrifuge.pptx
Centrifugation is a process that uses centrifugal force to separate mixtures based on density. Particles of different masses will settle at different rates in response to gravity when placed in a centrifuge. There are several types of centrifuges including low-speed, high-speed, and ultracentrifuges that can generate different centrifugal forces. Centrifugation techniques like density gradient centrifugation and differential centrifugation are used to separate components of a mixture based on properties like size, shape, and density. Centrifugation has many applications in clinical and research laboratories, such as separating blood components and isolating organelles or other cellular components.
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Cell centrifugation is an indispensable step in common cell experiments. It not only allows adherent samples to settle completely at the bottom of the tube, but also helps confirm cell concentration. The centrifuged samples can be used for subsequent experiments such as primary cell culture, cell passage culture, single cell sequencing, etc.What are the common approaches for cell centrifugation? Check out the detail steps for centrifugal separation and learn more.
Extraction techniques(cetrifugation)
i am HAFIZ M WASEEM from mailsi vehari
bsc in science college multan pakistan
msc univesity of education lahore pakistan
i love pakistan and my teachers
Centrifugation Presentation.pptx
A centrifuge operates by using the sedimentation principle- Here the substances are separated based on their density under the influence of gravitational force. When spun rapidly, lighter particles stay at the top and heavier particles go to the bottom during centrifugation.The components of heterogeneous mixtures are detached by centrifugation. That comprises liquids in liquids, solids in fluids, and gases in solids and liquids. In order to transfer bulky sections to the outside of the pipe, centrifugation uses centrifugal energy. It allows the solid to settle more easily and completely.The components of heterogeneous mixtures are detached by centrifugation. That comprises liquids in liquids, solids in fluids, and gases in solids and liquids. In order to transfer bulky sections to the outside of the pipe, centrifugation uses centrifugal energy. It allows the solid to settle more easily and completely.
What is the centrifuge used for?
Centrifuges work by separating out two materials with different densities. Centrifuges are used in various laboratories to separate fluids, gases or liquids based on density like the separation of different constituents of blood, immiscible liquids, wastewater sludge etc.
Centrifugation-1 (1)
The document discusses centrifugation, including its definition, principles, classifications, construction, advantages/disadvantages, and applications. Centrifugation uses centrifugal force to separate mixtures based on density differences. It can separate components in industry, water treatment, and pharmaceutical/biological analysis. Centrifuges are classified by speed, temperature, and use. Common techniques include density gradient, differential, and ultra centrifugation. Industrial and laboratory centrifuges have various uses such as wastewater treatment and isolating cell components.
Centrifugation 1 (1)
Centrifugation is a process that uses centrifugal force to separate particles in suspension. It works by spinning the suspension at high speeds, causing heavier particles to settle out of the liquid based on density and size differences. There are various types of centrifugation including differential, density gradient, and ultra centrifugation. Centrifuges are widely used in industries like pharmaceuticals, biotechnology, and wastewater treatment to separate mixtures and purify products. They provide advantages of a clean separation but also have high energy costs.
Centrifugation
Centrifugation is a process that uses centrifugal force to separate mixtures based on density. It works by spinning a liquid sample at high speeds, causing denser components to migrate outward and separate from less dense components. There are several types of centrifugation including density gradient centrifugation, differential centrifugation, and ultracentrifugation. Centrifugation has many applications such as separating immiscible liquids, purifying cells, and separating blood components.
Preparative and analytical centrifugation
1. Ultracentrifugation is a technique that uses very high speeds to separate particles in solution based on properties like size, shape, density.
2. There are two main types - preparative ultracentrifugation which handles large volumes to separate molecules, and analytical ultracentrifugation which uses small volumes and optical detection to study purified molecules.
3. Preparative ultracentrifugation techniques include differential centrifugation, density gradient centrifugation, and zonal centrifugation to separate organelles, proteins, and other molecules. Analytical ultracentrifugation determines molecular weight and detects conformational changes.
Centrifugation amirtham
A centrifuge uses centrifugal force to separate fluids or particles of different densities. It works by applying centrifugal acceleration, which causes denser particles to move outward in the radial direction while less dense particles move to the center. There are different types of rotors and centrifuges that separate particles based on properties like size, shape, density or sedimentation rate. Ultracentrifuges spin at very high speeds of over 100,000g to separate small particles like organelles, viruses and macromolecules.
Centrifugation
This document discusses centrifugation, including its principle, types of centrifuges, and applications. Centrifugation uses centrifugal force to separate mixtures based on density. There are three main types of centrifuges - low-speed, high-speed, and ultracentrifuges - which can achieve different revolutions per minute and relative centrifugal forces. Centrifugation techniques include preparative, which separates particles into pellets and supernatants, and analytical like differential and density gradient centrifugation for measuring properties of particles. Centrifugation is used widely in industry and research labs to separate components of mixtures.
Centrifugation techniques by kk sahu
Introduction
Definition
Components of centrifuge
Types of centrifugation
Application
Conclusion
References
A centrifugation is a technique for separating particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed.
Extraction techniques
I AM HAFIZ MUHAMMAD WASEEM from mailsi vehari
BSc from science college Multan
MSC university of education Lahore
i love Pakistan and my teachers
Ppt of centrifugation
Centrifugation is a process that uses centrifugal force to separate mixtures based on density differences. It works by spinning samples at high speeds, causing denser particles to sediment out of solution. There are two main types - preparative centrifugation which separates large amounts of samples, and analytical centrifugation which analyzes small amounts. Centrifugation has many applications including isolating suspensions, isotope separation, and separating cellular components.
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This document describes different types of centrifugation techniques used to separate particles based on their properties. It discusses analytical centrifugation which separates particles based on density and centrifugal force. Density gradient centrifugation uses a gradient medium to separate particles based on differences in buoyant density. Differential centrifugation separates particles into pellets of increasing size through successive centrifugation steps with increasing force.
Centrifugation notes slideshare
Centrifugation is a technique that uses centrifugal force to separate components of a solution based on properties like density, size, and shape. It works by spinning the solution at high speeds, allowing heavier components to sediment to the bottom. There are two main types - density gradient centrifugation, where particles separate through a density gradient, and differential centrifugation, which separates organelles from cells. Centrifugation has many applications, including separating cream from milk, clarifying wine, and purifying proteins and cells.
Ultra centrifugation
Ultracentrifugation is a technique that uses very high rotational speeds, up to 80,000 rpm, to separate particles via centrifugal force up to 600,000g. There are two main types: analytical ultracentrifugation monitors particles in real-time to study molecular interactions and properties, while preparative ultracentrifugation isolates and purifies particles like organelles. Common techniques include differential centrifugation to separate organelles and density gradient centrifugation to separate mixtures based on density.
Applied Biochemistry
This document discusses centrifugation, which uses centrifugal force to separate biological particles in liquid. It describes the basic principles of how centrifugation works based on gravity and centrifugal force. There are different types of rotors and centrifuges that are used for various purposes. Differential centrifugation separates particles based on size, while density gradient centrifugation uses layers of different density solutions to separate particles based on density. Analytical centrifugation uses high speeds and optical systems to analyze particles. Centrifugation has many applications including separating blood cells, precipitates, subcellular components, and more.
North south university
Centrifugation is one of
the most important and
widely applied research cellular,
techniques in bio-chemistry and
molecular biology, pharmacy and
in medicine.
Centrifuge.pptx
Centrifugation is a process that uses centrifugal force to separate mixtures based on density. Particles of different masses will settle at different rates in response to gravity when placed in a centrifuge. There are several types of centrifuges including low-speed, high-speed, and ultracentrifuges that can generate different centrifugal forces. Centrifugation techniques like density gradient centrifugation and differential centrifugation are used to separate components of a mixture based on properties like size, shape, and density. Centrifugation has many applications in clinical and research laboratories, such as separating blood components and isolating organelles or other cellular components.
Common Approaches for Centrifugation in the Laboratory.pdf
Cell centrifugation is an indispensable step in common cell experiments. It not only allows adherent samples to settle completely at the bottom of the tube, but also helps confirm cell concentration. The centrifuged samples can be used for subsequent experiments such as primary cell culture, cell passage culture, single cell sequencing, etc.What are the common approaches for cell centrifugation? Check out the detail steps for centrifugal separation and learn more.
Extraction techniques(cetrifugation)
i am HAFIZ M WASEEM from mailsi vehari
bsc in science college multan pakistan
msc univesity of education lahore pakistan
i love pakistan and my teachers
Centrifugation Presentation.pptx
A centrifuge operates by using the sedimentation principle- Here the substances are separated based on their density under the influence of gravitational force. When spun rapidly, lighter particles stay at the top and heavier particles go to the bottom during centrifugation.The components of heterogeneous mixtures are detached by centrifugation. That comprises liquids in liquids, solids in fluids, and gases in solids and liquids. In order to transfer bulky sections to the outside of the pipe, centrifugation uses centrifugal energy. It allows the solid to settle more easily and completely.The components of heterogeneous mixtures are detached by centrifugation. That comprises liquids in liquids, solids in fluids, and gases in solids and liquids. In order to transfer bulky sections to the outside of the pipe, centrifugation uses centrifugal energy. It allows the solid to settle more easily and completely.
What is the centrifuge used for?
Centrifuges work by separating out two materials with different densities. Centrifuges are used in various laboratories to separate fluids, gases or liquids based on density like the separation of different constituents of blood, immiscible liquids, wastewater sludge etc.
Centrifugation-1 (1)
The document discusses centrifugation, including its definition, principles, classifications, construction, advantages/disadvantages, and applications. Centrifugation uses centrifugal force to separate mixtures based on density differences. It can separate components in industry, water treatment, and pharmaceutical/biological analysis. Centrifuges are classified by speed, temperature, and use. Common techniques include density gradient, differential, and ultra centrifugation. Industrial and laboratory centrifuges have various uses such as wastewater treatment and isolating cell components.
Centrifugation 1 (1)
Centrifugation is a process that uses centrifugal force to separate particles in suspension. It works by spinning the suspension at high speeds, causing heavier particles to settle out of the liquid based on density and size differences. There are various types of centrifugation including differential, density gradient, and ultra centrifugation. Centrifuges are widely used in industries like pharmaceuticals, biotechnology, and wastewater treatment to separate mixtures and purify products. They provide advantages of a clean separation but also have high energy costs.
Centrifugation
Centrifugation is a process that uses centrifugal force to separate mixtures based on density. It works by spinning a liquid sample at high speeds, causing denser components to migrate outward and separate from less dense components. There are several types of centrifugation including density gradient centrifugation, differential centrifugation, and ultracentrifugation. Centrifugation has many applications such as separating immiscible liquids, purifying cells, and separating blood components.
Preparative and analytical centrifugation
1. Ultracentrifugation is a technique that uses very high speeds to separate particles in solution based on properties like size, shape, density.
2. There are two main types - preparative ultracentrifugation which handles large volumes to separate molecules, and analytical ultracentrifugation which uses small volumes and optical detection to study purified molecules.
3. Preparative ultracentrifugation techniques include differential centrifugation, density gradient centrifugation, and zonal centrifugation to separate organelles, proteins, and other molecules. Analytical ultracentrifugation determines molecular weight and detects conformational changes.
Centrifugation amirtham
A centrifuge uses centrifugal force to separate fluids or particles of different densities. It works by applying centrifugal acceleration, which causes denser particles to move outward in the radial direction while less dense particles move to the center. There are different types of rotors and centrifuges that separate particles based on properties like size, shape, density or sedimentation rate. Ultracentrifuges spin at very high speeds of over 100,000g to separate small particles like organelles, viruses and macromolecules.
Centrifugation
This document discusses centrifugation, including its principle, types of centrifuges, and applications. Centrifugation uses centrifugal force to separate mixtures based on density. There are three main types of centrifuges - low-speed, high-speed, and ultracentrifuges - which can achieve different revolutions per minute and relative centrifugal forces. Centrifugation techniques include preparative, which separates particles into pellets and supernatants, and analytical like differential and density gradient centrifugation for measuring properties of particles. Centrifugation is used widely in industry and research labs to separate components of mixtures.
Centrifugation techniques by kk sahu
Introduction
Definition
Components of centrifuge
Types of centrifugation
Application
Conclusion
References
A centrifugation is a technique for separating particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed.
Extraction techniques
I AM HAFIZ MUHAMMAD WASEEM from mailsi vehari
BSc from science college Multan
MSC university of education Lahore
i love Pakistan and my teachers
Ppt of centrifugation
Centrifugation is a process that uses centrifugal force to separate mixtures based on density differences. It works by spinning samples at high speeds, causing denser particles to sediment out of solution. There are two main types - preparative centrifugation which separates large amounts of samples, and analytical centrifugation which analyzes small amounts. Centrifugation has many applications including isolating suspensions, isotope separation, and separating cellular components.
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Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
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Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
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- 4. Types of Centrifugation ● Differential Centrifugation: Separates particles based on size and density. ● Density Gradient Centrifugation: Utilizes density gradients for finer separation. ● Ultracentrifugation: Highspeed, ultra-high-resolution technique for small particles.
- 5. 01 02 Differential Centrifugation Application: Isolation of organelles from cells, such as mitochondria and nuclei. Principle: Particles settle at different rates based on mass and size.
- 6. Density Gradient Centrifugation ● Principle: Particles move through a density gradient, separating according to buoyant density. ● Application: Isolation of biomolecules like RNA, DNA, and viral particles.
- 7. Utilizing Gradients Finer Separation of Particles
- 8. Ultracentrifugation ● Principle: Extremely high speeds for separating particles at a molecular level. ● Application: Study of macromolecules, proteins, and viruses.
- 9. High-Speed Precision Applications in Applied Biology
- 10. Separates components based on size and density Useful for studying subcellular structures Differential Centrifugation Requires multiple rounds of centrifugation at increasing speeds
- 11. Density Gradient Centrifugation ● Separates components based on density differences ● Creates density gradient using a medium ● Used for isolating organelles and viruses
- 12. 01 02 03 Equilibrium centrifugation technique Isopycnic Centrifugation Separates components based on density Commonly used for purifying nucleic acids
- 13. Ultracentrifugation ● Uses high centrifugal forces ● Can achieve separation of small particles ● Commonly used in biochemistry and molecular biology
Editor's Notes
- Entered text Title: Methods of Centrifugation Subtitle: Understanding the Basics Points: Definition of Centrifugation: A technique that uses centrifugal force to separate particles from a suspension. Importance in Applied Biology: Essential for isolating cellular components, proteins, and other biomolecules. Slide 2: Types of Centrifugation Title: Classification Subtitle: Overview of Methods Points: Differential Centrifugation: Separates particles based on size and density. Density Gradient Centrifugation: Utilizes density gradients for finer separation. Ultracentrifugation: High-speed, ultra-high-resolution technique for small particles. Slide 3: Differential Centrifugation Title: Technique and Application Subtitle: Separation Based on Size and Density Points: Principle: Particles settle at different rates based on mass and size. Application: Isolation of organelles from cells, such as mitochondria and nuclei. Slide 4: Density Gradient Centrifugation Title: Utilizing Gradients Subtitle: Finer Separation of Particles Points: Principle: Particles move through a density gradient, separating according to buoyant density. Application: Isolation of biomolecules like RNA, DNA, and viral particles. Slide 5: Ultracentrifugation Title: High-Speed Precision Subtitle: Applications in Applied Biology Points: Principle: Extremely high speeds for separating particles at a molecular level. Application: Study of macromolecules, proteins, and viruses. Entered text Methods of Centrifugation