5. ā¢ a localised area of death of tissue followed
later by degradation of tissue by hydrolytic
enzymes liberated from dead cells.
ā¢ Necrosis can be caused by various agents such
as hypoxia, chemical and physical agents,
microbial agents, immunological injury, etc.
6. ā¢ 5 types of necrosis:
ā¢ coagulative,
ā¢ liquefaction (colliquative),
ā¢ caseous,
ā¢ fat, and
ā¢ fibrinoid necrosis
9. ā¢ Apoptosis is a form of ācoordinated and internally
programmed cell deathā
ā¢ significance in a variety of physiologic and pathologic
conditions (apoptosis=falling off or dropping off , as
that of leaves or petals)
10. APOPTOSIS IN BIOLOGIC PROCESSES
ā¢ Apoptosis is responsible for mediating cell death in a wide
variety of physiologic and pathologic processes:
ā¢ Physiologic Processes:
ā¢ 1. Organised cell destruction in sculpting of tissues during
development of embryo.
ā¢ 2. Physiologic involution of cells in hormone-dependent
tissues
ā¢ 3. Normal cell destruction followed by replacement
proliferation such as in intestinal epithelium.
ā¢ 4. Involution of the thymus in early age.
11. Pathologic Processes:
ā¢ 1. Cell death in tumours exposed to chemotherapeutic agents.
ā¢ 2. Cell death by cytotoxic T cells in immune mechanisms such as in graft-versus-
host disease and rejection reactions.
ā¢ 3. Progressive depletion of CD4+T cells in the pathogenesis of AIDS.
ā¢ 4. Cell death in viral infections e.g. formation of Councilman bodies in viral
hepatitis.
ā¢ 5. Pathologic atrophy of organs and tissues on withdrawal of stimuli
ā¢ 6. Cell death in response to low dose of injurious agents involved in causation of
necrosis e.g. radiation, hypoxia and mild thermal injury.
ā¢ 7. In degenerative diseases of CNS e.g. in Alzheimerās disease, Parkinsonās disease,
and chronic infective dementias.
ā¢ 8. Heart diseases e.g. in acute myocardial infarction (20% necrosis and 80%
apoptosis).
12.
13.
14.
15. Necrosis and apoptosis.
A, Cell necrosis is identified
by homogeneous,
eosinophilic cytoplasm &
nuclear changes of pyknosis,
karyolysis, & karyorrhexis.
B, Apoptosis consists of
condensation of nuclear
chromatin and
fragmentation of the cell
into membrane-bound
apoptotic bodies which are
engulfed by macrophages.
18. ā¢ Two types of pathologic changes may superimpose
following cell injury:
ā¢ gangrene (after necrosis) &
ā¢ pathologic calcifi-cation (after degenerations as well as
necrosis)
20. ā¢ Gangrene is necrosis of tissue associated with
superadded putrefaction,
ā¢ most often following coagulative necrosis due to
ischaemia (e.g. in gangrene of the bowel, gangrene of
limb).
ā¢ 2 main types of gangreneādry and wet, and a variant of
wet gangrene called gas gangrene.
ā¢ In all types of gangrene, necrosis undergoes liquefaction
by the action of putrefactive bacteria.
21. Dry Gangrene
ā¢ This form of gangrene begins in the distal part of a limb
due to ischaemia.
ā¢ Th e typical example is the dry gangrene in the toes and
feet of an old patient due to severe atherossclerosis.
ā¢ Other causes of dry gangrene foot include
thromboangiitis obliterans (Buergerās disease),
Raynaudās disease, trauma, ergot poisoning
22. Dry gangrene of the foot. The gangrenous area is
dry, shrunken and dark and is separated from the
viable tissue by clear line of separation
23.
24. Wet Gangrene
ā¢ Wet gangrene occurs in naturally moist tissues and
organs such as the bowel, lung, mouth, cervix, vulva etc.
To other examples of wet gangrene having clinical
significance are as follows:
ā¢ Diabetic foot which is due to high glucose content in
the necrosed tissue which favours growth of bacteria.
ā¢ Bed sores occurring in a bed-ridden patient due to
pressure on sites like the sacrum, buttocks and heel.
25. ā¢ Wet gangrene usually develops due to blockage of
both venous as well as arterial blood fl ow and is more
rapid.
ā¢ The affected part is stuffed with blood which favours
the rapid growth of putrefactive bacteria.
ā¢ lacks clear-cut line of demarcation
ā¢ may spread to peritoneal cavity causing peritonitis.
28. GAS GANGRENE
ā¢ It is a special form of wet gangrene
ā¢ caused by gas-forming clostridia (gram-positive
anaerobic bacteria) which gain entry into the tissues
through open contaminated wounds, especially in the
muscles, or as a complication of operation on colon
which normally contains clostridia.
ā¢ Clostridia produce various toxins which produce
necrosis and oedema locally and are also absorbed
producing profound systemic manifes tations.
31. ā¢ Deposition of calcium salts in tissues other than osteoid
or enamel is called pathologic or heterotopic
calcification.
ā¢ Two distinct types:
ā¢ Dystrophic calcification is characterised by deposition
of calcium salts in dead or degenerated tissues with
normal calcium metabolism and normal serum calcium
level.
ā¢ Metastatic calcification, on the other hand, occurs in
apparently normal tissues and is associated with
deranged calcium metabolism and hypercalcaemia.
32. DYSTROPHIC CALCIFICATION
ā¢ As apparent from definition, dystrophic
calcification may occur due to 2 types of cause
ā¢ Calcification in dead tissue.
ā¢ Calcification of degenerated tissue.
33. Dystrophic calcification
in caseous necrosis in
tuberculous lymph
node.
In H & E, the deposits
are basophilic granular
while the periphery
shows healed
granulomas.
35. METASTATIC CALCIFICATION
ā¢ Since metastatic calcifi-cation occurs in normal tissues
due to hyper calcaemia, its causes would include either
of the following two groups of causes:
ā¢ Excessive mobilisation of calcium from the bone.
ā¢ Excessive absorption of calcium from the gut.
36. Excessive mobilisation of calcium
from the bone
ā¢ 1. Hyperparathyroidism
ā¢ 2. Bony destructive lesions
ā¢ 3. Hypercalcaemia
ā¢ 4. Prolonged immobilisation
37. Excessive absorption of calcium
from the gut
ā¢ 1. Hypervitaminosis D
ā¢ 2. Milk-alkali syndrome
ā¢ 3. Idiopathic hypercalcaemia of infancy (Williams
syndrome).
ā¢ 4. Renal causes such as in renal tubular acidosis.
38.
39.
40.
41. ADAPTATION:
ā¢ Cells may show adaptation to injury by various
processes like
ā¢ atrophy,
ā¢ hypertrophy,
ā¢ hyperplasia,
ā¢ metaplasia,
ā¢ dysplasia etc.
54. ā¢ 25. True about apoptosis
ā¢ (a) Migration of Leukocytes
ā¢ (b) End products are phagocytosed by
macrophage
ā¢ (c) Intranuclear fragmentation of DNA
ā¢ (d) Activation of caspases
ā¢ (e) Annexin V is a marker of apoptotic cell
55. ā¢ 26. Which of the following is the
hallmark of programmed cell death?
ā¢ (a) Apoptosis
ā¢ (b) Coagulation necrosis
ā¢ (c) Fibrinoid necrosis
ā¢ (d) Liquefaction necrosis