Cell biologists employ various fractionation procedures to isolate organelles from cells by first homogenizing, or breaking open, cells in a gentle manner. This allows each organelle to then be isolated using physical chemistry techniques such as sieving, sedimentation, precipitation, or ultracentrifugation of fluorescently labeled organelles in computer-generated density gradients in order to study their unique characteristics and differences from other organelles within the same cell.

3. :-

4. A host of fractionation procedures are employed by cell biologists.
Each organelle has characteristics
which make it different from other organelles within the
same cell. If the cell is broken open in a gentle manner, each of its
organelles can be subsequently isolated. The process of breaking
open cells is homogenization and the subsequent isolation of
organelles is fractionation. Isolating the organelles requires the
use of physical chemistry techniques, and those techniques can
range from the use of simple sieves, gravity sedimentation or
differential precipitation, to ultracentrifugation of fluorescent
labeled organelles in computer generated density gradient

13. •The terminal velocity is referred to
as the sedimentation velocity of the
particle and can be used to measure
the size, weight or density of the
particle.