This study examined changes in ant assemblages in three types of habitats following a wildfire in Greece. Pitfall traps were used to collect over 7,000 ant specimens from an unburned Abis forest, a burned Abis forest, and an unburned patch surrounded by burned forest over one year. The most abundant genus in the unburned forest was Formica, while three species dominated the burned forest. The results indicate the burned habitat supports less ant species than the unburned areas. Certain ant species and genera were found to characterize each habitat and could potentially serve as bioindicators for future similar studies.