This study examined the distribution of ant species across the Aegean Islands of Greece. The researcher conducted surveys at 57 stations across 3 islands, sampling different habitats. The results showed that the Mid-Aegean sea formed a barrier dividing the western and eastern islands into distinct ant communities. However, the expected gradient of European vs. Asian ant genera was not observed, indicating human transport has altered distributions. Across all islands, ant diversity increased with mild disturbance but declined with intense human pressure. Protected areas showed low diversity, suggesting habitat fragmentation is degrading conservation efforts. Further species-level identification and resampling is needed to fully evaluate patterns of composition and conservation impacts.