Tosoh Bioscience offers the G11 beta-thalassemia solution for laboratory diagnosis of haemoglobinopathies like beta-thalassemia. The solution allows for quantitative determination of HbF and HbA2 in 5 minutes with high resolution chromatographic separation of HbA2 and HbE. It provides a reliable, easy to use system with full reagent traceability and the ability to connect to automation lines. Haemoglobinopathies are among the most common inherited diseases worldwide due to global migration, and diagnosing variants like beta-thalassemia requires a number of laboratory tests on properties such as MCV, MCH, HbF, and HbA2 levels.

1. TOSOH BIOSCIENCE
G11
bThal
EN
Rev
10082017
-
10-2017
HLC-723G11
ß-THALASSAEMIA ANALYSIS MODE
THE ULTIMATE HAEMOGLOBINOPATHY
SOLUTION FOR YOUR LAB
CHROMATOGRAM INTERPRETATION AIDS
• Software to assist in the interpretation of chromatograms
• Tosoh Bioscience offers an on-line clinical interpretation platform under the supervision of
highly knowledgeable experts in the field
Example chromatograms: Normal, ß-Thalassaemia trait, HbS trait, HbE trait
References
1. Angastiniotis M, Vives Corrons J-L, Soteriades ES, Eleftheriou A. The Impact of Migrations on the Health Services
for Rare Diseases in Europe: The example of Haemoglobin Disorders, in: The Scientific World Journal Volume 2013
2. Haemoglobinopathies on the move: Is Europe ready? Report by group of experts from the European Network for
Rare and Congenital Anaemias (ENERCA) and the Thalassemia International Federation (TIF) in collaboration with
the International Organization for Migration (IOM), Migration Health Division, Regional Office Brussels
3. Weatherall DJ, Clegg JB. 2001. The thalassaemia syndromes. 4th Edition. Oxford: Blackwell Science Ltd 2001
4. Thein SL. The molecular basis of ß-thalassemia. Cold Spring Harb Perspect Med. 2013 May 1;3(5)
5. Cao A, Kan YW. The prevention of thalassemia. Cold Spring Harb Perspect Med. 2013 Feb 1;3(2)
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2. LABORATORY DIAGNOSIS TOSOH G11 BETA-THALASSAEMIA SOLUTION
Haemoglobinopathies are among the most common hereditary
diseases of the world’s population. About 4.5% of all human beings
carry a gene for a thalassaemia or haemoglobin anomaly(1)
. The areas
in which such abnormalities were originally most common extend
from Africa over the Mediterranean basin and the Near- and Middle
East to Southeast Asia and the Indian subcontinent. Global migration
in the modern period has led to a continual spread of these anomalies
to all regions of the world, with the result that they are rapidly
becoming more common in the industrialised regions of Northern and
Central Europe as well(2)
.
Variants of thalassaemias and main abnormal haemoglobins interact to produce a wide range
of clinical disorders of varying severity(3-4)
. Homozygotes for ß-thalassaemia may develop either
thalassaemia major or thalassaemia intermedia. Individuals with thalassaemia major are usually
diagnosed within the first 2 years of life and require regular blood transfusions to survive(5)
.
Thalassaemia
HbS
HbC
HbE
HbD
Diagnosis of Beta-Thalassaemia and other types of haemoglobinopathies should be done based on clinical
symptoms (if available) and a number of laboratory tests, such as MCV, MCH, total red cell count, HbF, HbA2
and iron markers.
As a guideline, the below scheme can be used (Adapted from Mosca et at. J.Clin.Pathol. 2009 – with permission)
• Quantitative determination of HbF and HbA2 in 5 minutes
• Chromatographic separation between HbA2 and HbE
• High resolution chromatogram thanks to Tosoh’s over
40 years’ experience in HPLC
• Full reagent traceability
• Easy to use and intuitive instrument
• Highly reliable system
• Instrument connectable to open laboratory automation lines
normal
or
reduced
normal
normal
or
reduced
<1%
<1% or
increased
<1%
altered normal
altered
iron
deficiency
iron
deficiency
normal
or
increased
normal
increased
or reduced
normal
or
increased
<1%
<1% or
increased
<1%
normal normal
normal normal
α-thal traits
Norm. HbA2
β thal carriers
α-gene triplications
Hb variants with
β or α-
thalassaemia
phenotype
Some unstable
Hb variants
α-thal carrier
Large β thal
deletions
δ β thalassaemia
γ δ β thalassaemia
α-Gap-PCR
α or δ sequence
α-MLPA
β-Gap-PCR
γ-sequence
β-MLPA
β-sequence
β or α-sequence
α-Gap-PCR
α-MLPA
Gap-PCR
α or δ sequence
α and β-MLPA
β-Gap-PCR
γ-sequence
β-MLPA
normal
or
reduced
normal
normal
or
reduced
reduced reduced reduced reduced
MCV
MCH
RBC
HbF
IRON
MARKERS
POSSIBLE
DIAGNOSIS
MOLECULAR
CONFIRMATION
normal
or
increased
normal
normal
or
increased
<1% <1-8%
<1% 3 - 16% 3 - 16%
normal normal normal
normal
δ or α-gene
defects
HPFH
High HbA2
β thal carrier
normal
reduced
< 2.3%
HbA2
normal
2.3 - 3.5%
borderline
3.6 - 4.0%
increased
4.1 - 8.0%
MLPA: Multiplex ligation-dependent probe amplification