Pharmaceutical analysis focuses on the identification, quantification, purification, and separation of substances and their components. It includes both qualitative and quantitative methods, such as instrumental techniques like spectroscopy and chromatography, as well as various chemical reactions. Applications of pharmaceutical analysis extend across the pharmaceutical industry, geographical surveys, and pollution control.

1. Introduction
of
Pharmaceutical Analysis

2. Introduction of
Pharmaceutical Analysis
• Introduction
– Definition
– Types of analysis
– Applications

5. Definition
• Pharmaceutical analysis
involves
• Identification
• Determination
• Quantification
• Purification of a substance
• Separation of components of
a solution or mixture
• Determination of structure
of chemical compounds

6. Types
1.Qualitative –
Identification
• Detection of evolved gas
• Formation of precipitates
• Limit tests
• Colour change reactions
• Melting point
• Boiling point test etc.,
1.Qualitative
2.Quantitative
analysis
This test is performed to indicate whether the compound or
substance is present in the sample or not.

7. Types
2. Quantitative –
Estimation
• Non-instrumental methods
Volumetric method
• Instrumental methods
– Electrochemical measurements
– Spectroscopy methods
• Absorption
• Emission
– Chromatography
• Bio assays and microbiological
assays
1.Qualitative
2.Quantitative
analysis
This test is used to quantify the compound or substance in the sample.

8. Chemical reactions
• Titrations
– Acid base titrations
– Complexometric titrations
– Redox titrations
– Non aqueous titrations
– Precipitation titrations
– Nitrimetic titrations
• Gravimetric methods
• Gasometric analysis

9. Electrochemical measurements
It uses measurement of potential, charge or
current to determine an analyte’s concentration.
• Potentiometry
• Conductometry
• Polarography
• Voltametry
• Amperometry

10. Spectroscopy methods
• Absorption
– UV-Visible
– Infra red
– Atomic absorption spectroscopy
– NMR
• Emission spectroscopy
– Flame emission spectroscopy
– Fluorimetry

11. • Chromatography methods
– Gas chromatography
– HPLC
– TLC
– HPTLC
– Paper chromatography
• Others
– Mass
– Refractive index
– Specific rotation etc.,

12. Bio assays and microbiological assays
• Potency of a substance is estimated using
micro organisms, animal tissues or intact
animals

13. Applications
• In pharma industry
– Quality control
– Quality assurance
– Analytical Research and development etc.,
• In geographical surveys
– To analyse soil samples
• In pollution control
– To analyse air samples, water, soil etc.,

14. Thank
you