This lab document outlines a procedure to demonstrate blood typing and compatibility using colored water and slides. Students will place drops of different colored water representing blood types (A, B, AB, and O) onto slides and observe if mixing causes color changes, indicating incompatible blood types. The purpose is to understand blood donor and receiver compatibility and identify which types can be safely transfused. Key conclusions are identifying which blood types are compatible for each receiver type and that type O blood is the "universal donor" while type AB is the "universal receiver".

1. Blood Tranfusion Lab
Name Period
PURPOSE: To demonstrate the concepts involved in blood transfusions and to form conclusions about
blood donors and receivers.
MATERIALS:
* 1 glass slide * 4 test tubes * red food coloring – type A
* 1 medicine dropper * blue food coloring – type B * red and blue (mixed) – type AB
PROCEDURE AND OBSERVATIONS:
1. Label the four test tubes A, B, AB, and O.
2. Place 4 mL of red food coloring from the type A beaker into test tube A. *4mL is about the width
of your finger.
3. Place 4 mL of blue food coloring from the type B beaker into test tube B.
4. Place 4 mL of the mixed food coloring from the type AB beaker into test tube AB.
5. Place 4 mL of water into test tube O.
6. Place 4 evenly spaced drops from test tube A onto 1 clean glass slide as shown in the picture
below.
7. Notice the data table on the other side of this paper. The drops on the slides represent blood
receiver types. The colored water in each test tube represents the blood donor types. You
will be working by columns (up and down) – filling up column A first.
8. Cleaning the dropper each time it is used, combine one drop from each donor tube with one drop
on the type A receiver slide.
9. If a combination produces a color change, the transfusion would not be safe. If there isn’t a color
change, that indicates a safe transfusion. Record your observations in the data table by writing
safe or unsafe in each box.
10. After recording your observations for the type A receiver slide, rinse the slide off and dry it.
11. Now place 4 evenly spaced drops from test tube B onto the glass slide.
12. Cleaning the dropper each time it is used, combine one drop from each donor tube with one drop
on the type B receiver slide.
13. Record your observations as you did for the type A receiver slide.
14. Repeat the same procedure for type AB and O receiver slides.

2. DATA TABLE
RECEIVER
A B AB O
A
D
O B
N
O A
R B
O
ANALYSIS AND CONDLUSIONS
1. What types of blood can a type A receiver be safely given in a transfusion?
2. What types of blood can a type B receiver be safely given in a transfusion?
3. What types of blood can a type AB receiver be safely given in a transfusion?
4. What types of blood can a type O receiver be safely given in a transfusion?
5. Type A blood has type antigens and antibodies.
6. Type B blood has type antigens and antibodies.
7. Type AB blood has type antigens and antibodies.
8. Type O blood has antigens and antibodies.
9. Which blood type might be considered a “universal donor”? This is because type
blood has no (antigens antibodies) and it will not react with (antigens antibodies).
Therefore moderate amounts of it can be transfused into people of all blood types.
10. Which blood type might be considered a “universal receiver”? EXPLAIN