BLIRT is a biotechnology company in Gdansk, Poland that offers outsourced R&D services including biology and chemistry services. It has modern laboratories and a team of over 30 employees, including 22 researchers of which 9 have PhDs. BLIRT has the equipment and expertise to handle projects of all sizes, from gene synthesis to protein production and purification to analytical testing. It works with clients through different collaboration models including fee-for-service, co-development, FTE, and consulting. Key benefits include competitive pricing, short deadlines, scientific expertise, and state-of-the-art facilities.

1. A flexible, affordable and fast R&D outsourcing service
partner

2. Company overview
BLIRT (BioLab Innovative Research Technologies) is a private biotechnology
company founded in 2008 with head office based in Gdansk, Poland.
BLIRT has a unique hybrid model, an innovative biopharmaceutical service
provider with Contract Research Organization. BLIRT offers a suite of biology
and chemistry services, attractive especially for discovery and lead optimization
development phase.
 BLIRT has modern laboratories and competent staff with a high percentage
of internationally educated PhDs.
We are able to accommodate small and bigger projects, and can engage
project resources on long term contract or as-needed basis.
www.blirt.eu
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3. Research Team
 891 publications in the field of life science
( Medicine, Microbiology, Molecular
Biology, Biotechnology, Biochemistry, Organic Chemistry, Analytical
Chemistry, Molecular Modeling)
BLIRT employes 34 people after 2 years activity.
The Research Team consists of 22 researchers where 9 are PhDs at
molecular biology, biotechnology, organic chemistry and computer
chemistry.
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4. Our machinery
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5. Protein Department
We offer a broad spectrum of custom services from gene to protein helping
you to speed up your development and to increase the efficiency.
Gene Production Purification Protein
In silico gene synthesis Proof of concept
Downstream Processing Formulation Adjustment
Metagenomics Difficult to express Optimization
proteins solutions Analytical testing
PCR Chromatography methods
E.coli, P.pastoris, development and validation
DNA and cDNA library B. megaterium, S.cerevisiae
construction Unique resin synthesis
Flask and Bioreactor (10L) for special proteins
Gene engineering expression optimizations
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6. Protein Department
Choosing the right expression strain of E.coli
Expression T7 system: OD600=0,3, IPTG to
1 2 3 4 5 6 M 7 8 9 10 11 12 1mM, 30oC, 6h, TB broth.
1. E.coli strain 1 protein I (whole fraction)
2. E.coli strain 1 protein I (soluble fraction)
3. E.coli strain 2 protein I (whole fraction)
4. E.coli strain 2 protein I (soluble fraction)
5. E.coli strain 3 protein I (whole fraction)
6. E.coli strain 3 protein I (soluble fraction)
M. Protein weight marker
(170, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa)
7. E.coli strain 4 protein I (whole fraction)
8. E.coli strain 4 protein I (soluble fraction)
9. E.coli strain 5 protein I (whole fraction)
10. E.coli strain 5 protein I (soluble fraction)
11. E.coli strain 6 protein I (whole fraction)
12. E.coli strain 6 protein I (soluble fraction)
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7. Protein Department
Screening for expression temperature Purification
M 1 2 3 4
M 1 2 3 4 5
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Expression T7 system: OD600=0,3, IPTG to 1mM, various oC, 6 h, TB broth. Expression T7 system: OD600=0,3, IPTG to 1mM, 37 oC, 6 h, TB broth.
M. Protein weight marker (170, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa) M. Protein weight marker (130, 90, 66, 40, 25, 10 kDa)
1. 18oC E.coli strain 1 protein II (whole fraction) 1. E.coli strain 2 protein III (soluble fraction)
2. 18oC E.coli strain 1 protein II (soluble fraction) 2. E.coli strain 2 protein III DEAE Sepharose week anion exchanger
3. 30oC E.coli strain 1 protein II (whole fraction) 3. E.coli strain 2 protein III Mono Q strong anion exchanger
4. 30oC E.coli strain 1 protein II (soluble fraction) 4. E.coli strain 2 protein III Octyl Sepharose HIC
5. 37oC E.coli strain 1 protein II (whole fraction) 5. Formulated protein III
6. 37oC E.coli strain 1 protein II (soluble fraction)
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8. Protein Department
1 2 M 4 5 6 7 8 9 10 11 12 13 14 15 Intracellular production of a therapeutic protein by E.coli.
Soluble protein
Loading buffer at various pH.
Elution stepwise with 50mM Hepes
1M NaCl pH=7,0 DEAE Sepharose
1. pH=4,5
2. pH=5,5
M. Protein weight marker
(170, 130, 100, 70, 55, 40, 35, 25, 15, 10
kDa)
4. pH=6,5
Loading buffer containing Triton X- 5. pH=7,5
100 (R) as additive at various pH. 6. pH=8,5
Elution stepwise with 50mM Hepes 7. pH=9,5
1M NaCl pH=7,0 8. pH=10,5
CM Sepharose
9. pH=4,5
10. pH=5,5
11. pH=6,5
Loading buffer containing L- 12. pH=7,5
Arginine as additive at various pH. 13. pH=8,5
Elution stepwise with 50mM Hepes 14. pH=9,5
1M NaCl pH=7,0 15. pH=10,5
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9. Analysis department
BLIRT specializes in development of new methods and analyses that are not
possible in diagnostics laboratories. We are also able to conduct all routine
tests, particularly those that require our unique equipment.
Example analyses that we are able to perform:
• Analysis of drugs and its metabolites concentration in blood, urine, other biological fluids and
tissues,
• Pharmacokinetic analysis after drug administration,
• ADME studies – complex analysis of absorption, distribution, metabolism and excretion,
• Analysis of purity, stability and protein binding of pharmaceuticals,
• Analysis of biochemical markers in blood, urine, other biological fluids and tissues,
• Determination of molecular weight and purity of proteins and peptides,
• Protein and peptide sequencing following partial proteolysis.
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10. Analysis department
BLIRT can provide you access to most advanced analytical equipment and high
profile scientists with many years of experience in biomedical analytics.
Overview of Analytical Methods:
Ultra-High Performance Lipid Chromatography with mass detection (UHPLC-MS/MS) connected to newest
generation of quadrupole time of flight mass detector (Q-TOF) with source of ions type electro-spray (ESI) or
chemical ionization under atmospheric pressure (APCI).
High Performance Lipid Chromatography (HPLC) with detection: RID, FL and UV-DAD.
Infrared spectroscopy with Fourier Transformation (IR).
Spectroscopy UV-VIS.
Detectors:
Overview of Analytical Equipment: micrOTOF-Q-II - Bruker
Spectrometer UV-VIS – Jasco RID – Shodex
Spectrometer IR- Jasco FL-Merck
HPLC-Merck UV-DAD - Dionex
UHPLC-Dionex FID- Perkin Elmer
PLC- Dionex ECD-Perkin Elmer
GC-Perkin- Elmer NPD- Perkin Elmer MS/MS - Bruker
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11. Synthesis department
BLIRT – Synthesis services
We offer all-embracing organic synthesis services directed to industrial and
scientific customers:
 Pharmaceutical Industry;
 Chemical Industry;
 Academic Scientists;
In particular we specialize in synthesis:
 chiral compounds with natural origin – with synthetic and bio-catalytic
methods;
 chiral unnatural/non-peptidic amino acids;
 peptides (‘on solid state’ and ‘in solution’);
 heterocyclic compounds (with N, O, S in ring structure);
 macrocyclic compounds;
 phosphoroorganic compounds;
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12. Synthesis department
We offer comprehensive organic synthesis and interdisciplinary chemistry
services:
 Custom synthesis - comprehensive equiped laboratory: mg – 100s g scale;
 Design of alternative synthetic pathways;
 Optimalization of synthetic routes;
 Reaction yields optimalization;
 Identification, synthesis and standarization of API impurities;
 Identification, synthesis and standarization of drug metabolites;
COOMe O OH
CHO A B
CH3 CH3
Br
COOMe COOMe
F CH3 F F
C
OH
CH3 E D CH3
CH3
O CN COOH
O COOH
F
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13. Collaboration models
 Fee for Service
 Co-development
 FTE
 Consulting
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14. Summary
Key benefits of collaboration
• Competitive pricing
• Short project deadlines
• Strong scientific team
• State of the art facilities
 Service Quality
• ISO 9001:2009 - certificate
• GLP - pending.- 03.2011
• GMP – planned for 2013
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