‘Omic’ technologies are primarily aimed at the universal detection of genes (genomics), mRNA (transcriptomics), proteins (proteomics) and metabolites (metabolomics) in a specific biological sample.

1. Transcriptomics
Biotech Era Ahead
Under the supervision of:
Prof. Dr. Fotoh El-Domiaty
Taha A. Taha | 4th Level
Biotechnology Program
Faculty of Agriculture
Ain Shams University

2. (-ome) Omics Family
• Introduction
‘Omic’ technologies are primarily aimed at the universal detection of genes
(genomics), mRNA (transcriptomics),

3. • 1983
• 1990
• 1995
• 2001
• 2003
• TimeLine

4. Human
Genome
Project

5. ACT AGTCCG GGTTTCCGT GCT ACG CAG GACACTAGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG
GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG
CAG GAC ACTAGTCCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT
ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT
GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACTAGTCCG GGTTTCCGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTC
CGT GCT ACG CAG GACACTAGTCCG GGTTTCCGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGT
TTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG
GGTTTC CGT GCTACG CAG GAC ACTAGTCCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGT
CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG CAG GAC ACT
AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG
GACACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG
CAG GAC ACTAGTCCG GGTTTCCGT GCTACG CAGGACACT AGTCCG GGTTTCCGT GCT ACG CAGGAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GACACT AGTCCG GGTTTCCGT GCT
ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT
GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACTAGTCCG GGTTTCCGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTC
CGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG CAG GACACTAGT CCG GGT
TTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG
GGTTTC CGT GCTACG CAG GAC ACTAGTCCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGT
CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG CAG GAC ACT
AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC
ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GACACT AGTCCG GGTTTCCGT GCT ACG CAG
GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG
CAG GAC ACTAGTCCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT
ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT
GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACTAGTCCG GGTTTCCGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTC
CGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GACACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGT
TTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG
GGTTTC CGT GCTACG CAG GAC ACTAGTCCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGT
CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG CAG GAC ACT
AGTCCG GGTTTCCGT GCT ACG CAGGACACT AGTCCG GGTTTCCGT GCT ACGCAG GACACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC
ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GACACTAGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG
GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCTACG
CAG GAC ACTAGTCCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT
ACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTCCGT GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT
GCT ACG CAG GAC ACTAGT CCG GGTTTC CGT GCTACG CAG GAC ACTAGTCCG GGTTTCCGT GCTACG CAG GAC ACT AGTCCG GGTTTCCGT GCT ACG CAG GAC ACT AGTCCG GGTTTC
CGT GCT ACG CAG GAC
Human
Genome
Project
The Human Genome Project was an international
research effort to determine the sequence of the
human genome and identify the genes that it contains.

6. • 1995
• 2002
• 2008
• TimeLine Of Transcriptomics:

7. • 1 May 2008
• 2 May 2008
• 18 May 2008
 Nagalakshmi andWang et al., Science | Online: 5/1/2008 In
print:6/6/2008: TheTranscriptional Landscape of theYeast
Genome Defined
 Lister, O’Malley,Tonti-Filippini et al., Cell | Online: 5/2/2008 In
print: 5/2/2008: Highly Integrated Single-Base Resolution Maps
of the Epigenome in Arabidopsis
 Wilhelm and Marguerat et al., Nature | Online: 5/18/2008 In
print:6/26/2008: Dynamic repertoire of a eukaryotic
transcriptome surveyed at single-nucleotide resolution
• First Published Papers on Transcriptomics:

8. •
Dictiona
ry:
•
Glossar
y:
 Transcriptomics: The study of the RNA transcripts of a
cell, tissue, or organism (i.e. the transcriptome).
Transcriptomics is concerned with determining how
the transcriptome, and hence pattern of gene
expression, changes with respect to various factors,
such as type of tissue, stage of development,
hormones, drugs, or disease. It complements and
overlaps with proteomics.
 Transcriptome: The complete complement of mRNA
molecules generated by an organism or cell type.
• Definition

9. • References:
 http://rnaseq.uoregon.edu/index.html
 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949280/
 https://en.wikipedia.org/wiki/RNA-Seq
 http://www.cpgr.org.za/services/genomics-transcriptomics/
 http://onlinelibrary.wiley.com/doi/10.1576/toag.13.3.189.27672/full
 http://metabolomics.se/sites/default/files/courses_files/History%20of%20Omics%
20cascade_Wheelock.pdf
 https://unlockinglifescode.org/timeline
 http://www.yourgenome.org/facts/timeline-history-of-genomics
 https://books.google.com.eg/books?id=Nx0oCAAAQBAJ&pg=PA16-
IA24&lpg=PA16-
IA24&dq=timeline+of+transcriptomics&source=bl&ots=T9DtVyRgJc&sig=vQJdtUs
vWpC7uf7QJQ5mDJT2-
28&hl=ar&sa=X&ved=0ahUKEwiqwIX87uDSAhVFthQKHREHBJ4Q6AEIJDAA#v=o
nepage&q&f=false
 https://en.wikipedia.org/wiki/Omics
 http://www.sciencedirect.com/science/article/pii/S0092867408004480
 http://www.encyclopedia.com/science/dictionaries-thesauruses-pictures-and-
press-releases/transcriptomics